##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937123_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 753847 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.950633218676998 31.0 31.0 34.0 30.0 34.0 2 31.943748532527156 31.0 31.0 34.0 30.0 34.0 3 32.116264971539316 33.0 31.0 34.0 30.0 34.0 4 35.902106130289035 37.0 35.0 37.0 35.0 37.0 5 35.81682357295313 37.0 35.0 37.0 35.0 37.0 6 35.861954746785486 37.0 35.0 37.0 35.0 37.0 7 35.79697737073968 37.0 35.0 37.0 33.0 37.0 8 35.78410605865646 37.0 35.0 37.0 33.0 37.0 9 37.41834881613909 39.0 37.0 39.0 34.0 39.0 10-11 37.341770942910166 39.0 37.0 39.0 34.0 39.0 12-13 37.23189519889314 39.0 37.0 39.0 33.0 39.0 14-15 38.34168869810452 40.0 38.0 41.0 33.0 41.0 16-17 38.286636412959126 40.0 38.0 41.0 33.0 41.0 18-19 38.39553450501229 40.0 38.0 41.0 33.5 41.0 20-21 38.47085283883865 40.0 38.0 41.0 34.0 41.0 22-23 38.38228048927701 40.0 38.0 41.0 34.0 41.0 24-25 38.25182231938311 40.0 38.0 41.0 33.5 41.0 26-27 38.05943580063328 40.0 37.0 41.0 33.0 41.0 28-29 37.876697791461666 40.0 37.0 41.0 33.0 41.0 30-31 37.6845314765463 39.5 37.0 41.0 33.0 41.0 32-33 37.60126723327147 40.0 36.0 41.0 33.0 41.0 34-35 37.46165932874973 40.0 36.0 41.0 33.0 41.0 36-37 37.24234028920988 40.0 36.0 41.0 32.0 41.0 38-39 37.06132676789853 39.0 35.5 41.0 31.0 41.0 40-41 36.86240112383547 39.0 35.0 41.0 31.0 41.0 42-43 36.56242447074804 39.0 35.0 41.0 30.5 41.0 44-45 36.06384783649733 39.0 35.0 41.0 30.0 41.0 46-47 35.698154930642424 39.0 35.0 41.0 28.5 41.0 48-49 35.24875472078552 39.0 35.0 40.0 26.5 41.0 50-51 34.93083278171831 39.0 35.0 40.0 25.0 41.0 52-53 34.67614980228083 38.0 34.0 40.0 24.0 41.0 54-55 34.419501569947215 38.0 34.0 40.0 23.5 41.0 56-57 34.125106951410565 38.0 34.0 40.0 23.0 41.0 58-59 33.78295662117114 38.0 33.0 40.0 22.0 41.0 60-61 33.42940477311709 37.0 33.0 40.0 21.5 41.0 62-63 33.05987090218572 36.5 32.5 39.5 19.5 41.0 64-65 32.676856179038985 36.0 32.0 39.0 18.0 40.5 66-67 32.28241208096603 35.5 31.5 39.0 17.0 40.0 68-69 31.8191861213217 35.0 31.0 38.5 13.0 40.0 70-71 31.32789146869325 35.0 31.0 38.0 9.0 40.0 72-73 30.97706165840018 35.0 30.5 37.0 7.5 39.0 74-75 30.536915978971862 34.5 30.0 36.5 7.0 39.0 76-77 29.136079337053808 33.0 28.5 35.0 2.0 37.5 78-79 29.861988573278133 34.0 29.5 36.0 2.0 38.0 80-81 29.691765039855568 34.0 30.0 35.5 2.0 37.0 82-83 29.347934660481503 34.0 29.0 35.0 2.0 37.0 84-85 28.967229424538402 34.0 29.0 35.0 2.0 36.0 86-87 28.606160799207267 34.0 29.0 35.0 2.0 36.0 88-89 28.222944443633786 34.0 29.0 35.0 2.0 35.5 90-91 27.78782498305359 33.5 27.0 35.0 2.0 35.0 92-93 27.328526212878742 33.0 26.5 35.0 2.0 35.0 94-95 26.824588411176272 33.0 25.5 35.0 2.0 35.0 96-97 26.133663727520307 33.0 24.5 34.5 2.0 35.0 98-99 25.432020025283645 32.0 22.0 34.0 2.0 35.0 100-101 22.68198984674609 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5348592324923658 1101 2 0.4467810161745227 1101 3 0.44797496518432567 1101 4 0.33212144891453477 1101 5 0.46386609094957265 1101 6 0.4623064618960555 1101 7 0.516004120558982 1101 8 0.49162378175786614 1101 9 0.5733521494697627 1101 10-11 0.5409220240087365 1101 12-13 0.6071312473201118 1101 14-15 0.8939805955873439 1101 16-17 0.7668927251603463 1101 18-19 0.8006576569978279 1101 20-21 0.8268846225711002 1101 22-23 0.6867696413960687 1101 24-25 0.7024279226120882 1101 26-27 0.793469286036931 1101 28-29 0.8174288097387148 1101 30-31 0.7843732971906405 1101 32-33 0.9845732871471284 1101 34-35 0.9248005340877583 1101 36-37 1.0373180617460136 1101 38-39 0.9865244683351975 1101 40-41 0.9609472323102821 1101 42-43 1.0858076083764985 1101 44-45 1.362712976766133 1101 46-47 1.459248215203658 1101 48-49 1.3115081614870974 1101 50-51 1.398742984944498 1101 52-53 1.3830542357883715 1101 54-55 1.3768035184029301 1101 56-57 1.5662596166036096 1101 58-59 1.414811249659131 1101 60-61 1.529938472521117 1101 62-63 1.6902995463201265 1101 64-65 1.542465122310844 1101 66-67 1.550591835574611 1101 68-69 1.5084859358245204 1101 70-71 1.4162858801508769 1101 72-73 1.5754992206409462 1101 74-75 1.5903480651569701 1101 76-77 1.39216483087964 1101 78-79 1.5272459315604188 1101 80-81 1.692668351930113 1101 82-83 1.7169945455586841 1101 84-85 1.6838350905155473 1101 86-87 1.500247085224153 1101 88-89 1.5373318311205217 1101 90-91 1.6919357055622228 1101 92-93 1.8783454120193852 1101 94-95 1.8634630641565444 1101 96-97 1.775845704451715 1101 98-99 1.7207685761509026 1101 100-101 1.606161876351731 1102 1 0.49726174080520025 1102 2 0.38282328490791784 1102 3 0.47019172512987595 1102 4 0.3510004703222336 1102 5 0.39249812899355163 1102 6 0.3985683274205485 1102 7 0.438445983801266 1102 8 0.4242045814351698 1102 9 0.5166576981698299 1102 10-11 0.5601491826252882 1102 12-13 0.6044179664246343 1102 14-15 0.846125744422217 1102 16-17 0.8223891797668443 1102 18-19 0.7192116287142909 1102 20-21 0.6308294699413821 1102 22-23 0.6885783294677879 1102 24-25 0.7752036923950456 1102 26-27 0.7526997485568643 1102 28-29 0.7692148746968996 1102 30-31 0.7595902070723639 1102 32-33 0.8376519213745013 1102 34-35 0.8978116904008644 1102 36-37 0.9495956121869327 1102 38-39 0.9789309638888639 1102 40-41 0.9565314128493654 1102 42-43 1.0984609612413365 1102 44-45 1.2278559999571428 1102 46-47 1.3177544063736093 1102 48-49 1.3110462452762164 1102 50-51 1.3829923885169606 1102 52-53 1.5838060064948252 1102 54-55 1.5355300361418358 1102 56-57 1.5887862004205928 1102 58-59 1.7130887085460955 1102 60-61 1.7159183784195733 1102 62-63 1.7087652480877793 1102 64-65 1.635582454667606 1102 66-67 1.586283611906989 1102 68-69 1.531122152178444 1102 70-71 1.392528791354163 1102 72-73 1.6215377542385916 1102 74-75 1.4956658568757142 1102 76-77 1.4792283242068187 1102 78-79 1.6744618622129472 1102 80-81 1.7354913847807332 1102 82-83 1.7053675257326084 1102 84-85 1.6880109125771305 1102 86-87 1.682179211902973 1102 88-89 1.7125659239535729 1102 90-91 2.1059968994257225 1102 92-93 2.043900991900866 1102 94-95 1.991786372995879 1102 96-97 2.1276699500604437 1102 98-99 2.27701852639332 1102 100-101 2.27852562019174 1103 1 0.2233070460461377 1103 2 0.06092274255057717 1103 3 0.17432485614979853 1103 4 0.19405451161361498 1103 5 0.18562196146693566 1103 6 0.29431037087858414 1103 7 0.33216199966226156 1103 8 0.382625877120617 1103 9 0.43933184626602184 1103 10-11 0.47721269260091503 1103 12-13 0.43915045092127514 1103 14-15 0.5305810607047405 1103 16-17 0.5700302551760359 1103 18-19 0.6631004732361987 1103 20-21 0.4658054423304563 1103 22-23 0.4768944696334785 1103 24-25 0.5147428275399335 1103 26-27 0.5273831708942041 1103 28-29 0.5903111691670802 1103 30-31 0.5861057347423113 1103 32-33 0.7357902155530098 1103 34-35 0.7092166459220124 1103 36-37 0.7876805039585193 1103 38-39 0.8597811836790044 1103 40-41 0.6600942353297654 1103 42-43 0.8081287760481857 1103 44-45 0.9903020940814855 1103 46-47 0.8008955824765494 1103 48-49 0.9800828318276942 1103 50-51 0.9437419666448932 1103 52-53 1.0841267507952068 1103 54-55 1.0664165404022228 1103 56-57 1.1452068155552055 1103 58-59 1.353290859920051 1103 60-61 1.2280866966951365 1103 62-63 1.3161525231761502 1103 64-65 1.3165994267719725 1103 66-67 1.4291223769155614 1103 68-69 1.2599638789720053 1103 70-71 1.2818572508787511 1103 72-73 1.3461286424963141 1103 74-75 1.1430897462931462 1103 76-77 1.2386805155110459 1103 78-79 1.4329874106466391 1103 80-81 1.4857335705988852 1103 82-83 1.39176293270895 1103 84-85 1.4429939508893632 1103 86-87 1.3638893215549892 1103 88-89 1.70703427834 1103 90-91 1.9626033639641598 1103 92-93 1.7659953697176896 1103 94-95 1.7320221178865403 1103 96-97 1.9394377502840534 1103 98-99 2.0460531164308904 1103 100-101 2.0573643956169114 1104 1 0.28824028687712655 1104 2 0.13890704268947118 1104 3 0.25281635856095974 1104 4 0.22912595264975266 1104 5 0.28222519592775086 1104 6 0.26916306940748314 1104 7 0.2930017280004691 1104 8 0.24617427840486528 1104 9 0.3078806235183933 1104 10-11 0.33389577417747773 1104 12-13 0.35337935449892655 1104 14-15 0.4268738022500784 1104 16-17 0.4442990681240957 1104 18-19 0.4532723119473019 1104 20-21 0.5253669500051714 1104 22-23 0.4212306163433226 1104 24-25 0.342543045175816 1104 26-27 0.349676519663511 1104 28-29 0.5056293162259493 1104 30-31 0.472589255279388 1104 32-33 0.5822916548724706 1104 34-35 0.2824839649288293 1104 36-37 0.687302691468723 1104 38-39 0.6284685725733326 1104 40-41 0.5069507162398068 1104 42-43 0.6129243557981141 1104 44-45 0.5588612991288215 1104 46-47 0.7382057363113574 1104 48-49 0.6202214009072264 1104 50-51 0.6800418303032458 1104 52-53 0.9196956756824548 1104 54-55 0.7355319654519121 1104 56-57 0.7759614332240048 1104 58-59 0.6561611991567915 1104 60-61 0.7466715810743025 1104 62-63 0.7713456360077302 1104 64-65 0.8623214061518922 1104 66-67 0.8329844916723808 1104 68-69 0.672061468945433 1104 70-71 0.773564316383748 1104 72-73 0.9106802402189409 1104 74-75 0.7160541626842232 1104 76-77 0.7798341275715366 1104 78-79 0.7403381548507006 1104 80-81 1.101513661881107 1104 82-83 1.2252587126441696 1104 84-85 1.1870880592561441 1104 86-87 1.1717834460954926 1104 88-89 1.3452220694492745 1104 90-91 1.3885475759704562 1104 92-93 1.489561973533835 1104 94-95 1.2993023219591961 1104 96-97 1.5749033194075643 1104 98-99 1.622956685574266 1104 100-101 1.3209350712387113 1105 1 0.12174889913990583 1105 2 -0.1716231760721172 1105 3 0.02586048166070043 1105 4 0.07578048462130482 1105 5 0.039716684787428846 1105 6 0.050944172990590175 1105 7 0.10003648041898572 1105 8 0.1880760547894056 1105 9 0.19668299652543908 1105 10-11 0.11352802550050001 1105 12-13 0.24628055785619551 1105 14-15 0.2017903762211546 1105 16-17 0.3550618685420659 1105 18-19 0.17277883434591956 1105 20-21 0.33727917571050625 1105 22-23 0.35663021859498656 1105 24-25 0.28338303573772095 1105 26-27 0.24346928603691964 1105 28-29 0.15207317640729912 1105 30-31 0.27861278310491855 1105 32-33 0.28428545269497363 1105 34-35 0.2472411024890775 1105 36-37 0.21611585087142515 1105 38-39 0.17054057176435578 1105 40-41 0.07687184072155873 1105 42-43 0.07119785834302661 1105 44-45 0.4483614171050192 1105 46-47 0.584786111812754 1105 48-49 0.7228876630840517 1105 50-51 0.7882967937306233 1105 52-53 0.929206242979447 1105 54-55 1.0613350463161737 1105 56-57 1.125511438280121 1105 58-59 1.0318479891928618 1105 60-61 1.3768296691856747 1105 62-63 1.1449240227674267 1105 64-65 1.0001496328010262 1105 66-67 1.0948781782601777 1105 68-69 0.8584065661915297 1105 70-71 1.0064105765381264 1105 72-73 1.1508831592872646 1105 74-75 1.0847635030287144 1105 76-77 1.06502137265867 1105 78-79 1.2448937216420823 1105 80-81 1.2265448413060547 1105 82-83 1.2531937920253036 1105 84-85 1.5909745324418374 1105 86-87 1.4877152895579258 1105 88-89 1.560754479197481 1105 90-91 1.5763572635020573 1105 92-93 1.441034990690948 1105 94-95 1.3824266688766471 1105 96-97 1.8144556839877382 1105 98-99 1.7146140826821608 1105 100-101 1.3885590305200708 1106 1 -0.011209111650615 1106 2 0.23513262969846593 1106 3 0.3025201110296898 1106 4 0.04495125637828323 1106 5 0.010101390393806753 1106 6 0.0283896339335854 1106 7 -0.02937942387377035 1106 8 -0.11220543711770148 1106 9 -0.005263989165392502 1106 10-11 0.042841302535450154 1106 12-13 0.16135210371591313 1106 14-15 0.23837259531568833 1106 16-17 0.06261230250876082 1106 18-19 -0.03097145887481645 1106 20-21 -0.1016429448618581 1106 22-23 0.017916284745588484 1106 24-25 0.04234679365467997 1106 26-27 -0.03136064593586241 1106 28-29 0.11030095027545883 1106 30-31 0.25384735969417704 1106 32-33 0.1573472636262352 1106 34-35 0.1771789396929293 1106 36-37 0.07925213986977298 1106 38-39 0.21681666840991198 1106 40-41 0.46245124489701794 1106 42-43 0.2011914074868244 1106 44-45 0.6367821302178527 1106 46-47 0.7139498462720582 1106 48-49 0.6118713600111008 1106 50-51 0.8597341617812901 1106 52-53 0.7387652389916468 1106 54-55 1.4609157406628839 1106 56-57 1.2295256301637778 1106 58-59 1.3403895092491638 1106 60-61 1.2439185737130032 1106 62-63 1.4490555029457042 1106 64-65 1.6659688915390092 1106 66-67 1.6107502167307395 1106 68-69 1.0291020857786712 1106 70-71 1.066016487868179 1106 72-73 0.8762614140374403 1106 74-75 0.9598661302865139 1106 76-77 1.046744924148232 1106 78-79 1.1502332712550363 1106 80-81 0.8963460376466621 1106 82-83 1.1880108222856798 1106 84-85 1.0396550391272719 1106 86-87 0.6655243442142726 1106 88-89 1.3231020044144621 1106 90-91 1.4235012935278597 1106 92-93 1.2865164591403975 1106 94-95 1.49928686122945 1106 96-97 1.8968331177198507 1106 98-99 1.8693495975777736 1106 100-101 1.6799207476207023 1107 1 -0.09919430572824695 1107 2 -0.16722831467928145 1107 3 -0.24014695579704082 1107 4 0.036547895033741895 1107 5 0.1546592135230611 1107 6 -0.057794839855930036 1107 7 0.11492829921546388 1107 8 -0.08114301214771302 1107 9 6.383717789830712E-4 1107 10-11 0.16131369149103136 1107 12-13 0.10943133540858696 1107 14-15 0.07808347966942364 1107 16-17 0.1780334709761462 1107 18-19 0.0984803218374708 1107 20-21 0.07405233324938365 1107 22-23 -0.04680960561055514 1107 24-25 0.002881007340157282 1107 26-27 0.04346928603693101 1107 28-29 -0.13509720898824185 1107 30-31 -0.030616425820028326 1107 32-33 -0.01932340464107085 1107 34-35 -0.11188668655757539 1107 36-37 -0.2869943587296646 1107 38-39 -0.33502906988540815 1107 40-41 -0.20681646218582017 1107 42-43 -0.3712125540978093 1107 44-45 -0.4937700906705018 1107 46-47 -0.6851597975854844 1107 48-49 -0.887178259836837 1107 50-51 -0.6798066545452386 1107 52-53 -0.4027663736534066 1107 54-55 -0.2735280368481199 1107 56-57 -0.18471506611472677 1107 58-59 -0.143904208237835 1107 60-61 -0.32841035785962447 1107 62-63 -0.5939366939330455 1107 64-65 -0.6632377911340654 1107 66-67 -0.3678162098398907 1107 68-69 -0.10160019513368823 1107 70-71 -0.04515297991893519 1107 72-73 -0.4094278616728424 1107 74-75 -0.3776688837190889 1107 76-77 -0.12204759285857136 1107 78-79 -0.2010072249434458 1107 80-81 -0.5804446786398536 1107 82-83 -0.3281956603073546 1107 84-85 -0.3709592065710048 1107 86-87 -0.44944664418108715 1107 88-89 -0.4207955546091462 1107 90-91 -0.054389862934723254 1107 92-93 -0.15320943121574615 1107 94-95 -0.40825115397663225 1107 96-97 -0.0033519563097605953 1107 98-99 -0.0011736117059371054 1107 100-101 0.3600177864362273 1108 1 -0.2774254573438242 1108 2 -0.42765361155859694 1108 3 -0.2617224874181403 1108 4 -0.2426912654191682 1108 5 -0.11294642027975499 1108 6 -0.20129801577418505 1108 7 -0.16437990293863436 1108 8 -0.038716886767986125 1108 9 -0.08421387898047072 1108 10-11 -0.0762242814446239 1108 12-13 -0.016500727557442474 1108 14-15 -0.18746816352305729 1108 16-17 -0.1251631597888334 1108 18-19 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-0.3361143447074717 2316 96-97 -0.0673559670049464 2316 98-99 0.02138654872187118 2316 100-101 0.06690281317419533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 9.0 5 18.0 6 59.0 7 137.0 8 327.0 9 630.0 10 991.0 11 1687.0 12 2610.0 13 3572.0 14 4430.0 15 6142.0 16 8309.0 17 11261.0 18 9893.0 19 5700.0 20 4770.0 21 4959.0 22 5009.0 23 5475.0 24 6275.0 25 7555.0 26 9058.0 27 10920.0 28 13224.0 29 16379.0 30 20179.0 31 24751.0 32 30648.0 33 39015.0 34 51733.0 35 71712.0 36 104901.0 37 151693.0 38 111405.0 39 8407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32082641207033 20.134904924401233 15.573372463898869 20.970896199629568 2 15.356268289641594 22.28185747145954 39.45481944492347 22.907054793975394 3 19.01187794660363 26.847953262919955 31.06081710296962 23.0793516875068 4 12.675781690449123 18.198918348152873 38.08345725326227 31.041842708135736 5 12.875911672861665 38.77511494573076 33.67399188680797 14.674981494599606 6 29.44419461346726 38.00128143393253 17.564134707094183 14.990389245506028 7 26.581388531094507 34.96611381354572 21.21849659148342 17.234001063876356 8 26.67570475175997 33.586390872418406 20.078079504196474 19.659824871625144 9 26.851469860595056 16.758042414442187 21.131741586820667 35.25874613814209 10-11 24.431316210472694 27.445923968555913 27.18386779262608 20.938892028345315 12-13 26.35139302971111 25.44967947548725 27.931762934565246 20.267164560236388 14-15 23.01077008995194 25.819695508505042 27.84523915330299 23.324295248240027 16-17 20.753415480860173 29.502471987021238 27.988504298617624 21.75560823350096 18-19 20.72177776126986 27.32563769571279 31.70643379890084 20.246150744116512 20-21 21.990045527385412 25.871215337104285 31.783142663086732 20.355596472423564 22-23 21.5427666356701 26.208434868083312 31.84671425368808 20.4020842425585 24-25 21.635579386815262 26.290214421641284 31.901910075170615 20.17229611637284 26-27 21.24326934429252 26.365266831779703 32.139867635412365 20.251596188515403 28-29 20.677461998226352 26.764909357553297 32.06489403546103 20.492734608759328 30-31 20.958466750381927 26.246922867201196 32.02984296423661 20.764767418180273 32-33 20.859930489228486 26.62076833280272 31.85019367505041 20.66910750291839 34-35 21.415206284204654 26.363360799865752 31.58080805065887 20.64062486527072 36-37 21.590443278523246 25.96446077618514 31.748041628250963 20.697054317040653 38-39 21.19020677158022 26.587731667876053 31.637895697553947 20.584165862989785 40-41 21.074254907202096 26.664150577959727 31.59022333714284 20.671371177695338 42-43 21.06578194198571 26.857527179482414 30.869697007885716 21.20699387064616 44-45 21.354740117955753 26.828897832702342 30.544530551494876 21.271831497847028 46-47 21.72405124916014 27.188426291028744 30.092333120867444 20.99518933894367 48-49 21.893876132443584 27.326023701143004 29.503724569557026 21.276375596856383 50-51 21.37327874212952 27.524406526270965 29.633260396195244 21.46905433540427 52-53 21.777285778622932 28.0030059409119 28.93244658518612 21.287261695279042 54-55 21.495919105088433 27.642300689490497 29.118055762833844 21.74372444258723 56-57 21.810399890163023 27.886255056579973 28.80673666784065 21.496608385416355 58-59 22.210497062040393 27.400262257416863 28.850769958326673 21.53847072221607 60-61 21.90602654009701 27.423705677792583 29.499078385945037 21.171189396165367 62-63 21.506154431867476 27.52428543192452 29.58385454873469 21.385705587473318 64-65 21.406015680911167 27.688793408207108 29.28918553047603 21.616005380405692 66-67 21.60823084790415 27.717958683923925 28.804187056524732 21.86962341164719 68-69 21.490766694037383 27.557780292287426 29.050722494086994 21.900730519588194 70-71 21.710041958116168 27.638565915895402 28.864676784546468 21.786715341441965 72-73 21.705531759097006 27.422010036519346 29.033742921308964 21.838715283074684 74-75 21.42183451991993 28.185842410157026 28.434965901387955 21.957357168535093 76-77 21.49681232937406 27.897623109351827 28.510417302299423 22.09514725897469 78-79 21.492003334900854 27.885422145519208 28.385391966921603 22.237182552658336 80-81 21.48757808882857 28.11051899138884 28.229451477800815 22.172451441981774 82-83 21.512073305409356 27.810886370646 28.4960518417553 22.180988482189345 84-85 21.16964649931847 27.88446805758688 28.16590222434475 22.779983218749898 86-87 21.236415251398686 27.857713749407196 28.150484358262613 22.75538664093151 88-89 21.384160480528923 27.587764447439493 27.932401742554084 23.0956733294775 90-91 21.23458984632793 27.57236064949648 28.287427596238224 22.905621907937366 92-93 21.27739448455721 27.75211680884848 28.17766735159787 22.792821354996438 94-95 20.982269588218283 27.5617301146388 28.077153689215038 23.378846607927876 96-97 21.005287636270054 27.707890596603495 28.120116630451797 23.166705136674654 98-99 20.975131215620777 27.944864565845933 27.4772441303686 23.602760088164693 100-101 20.532579151306443 27.759633662801576 27.75419485010765 23.95359233578434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 938.0 1 666.0 2 411.5 3 450.5 4 414.0 5 382.5 6 605.0 7 1227.0 8 1995.5 9 2339.5 10 2057.0 11 1706.0 12 1658.0 13 1691.5 14 1798.5 15 1936.5 16 1994.5 17 2092.0 18 2189.5 19 2230.5 20 2393.5 21 2684.5 22 3009.5 23 3387.0 24 3706.5 25 4372.0 26 5438.5 27 6346.5 28 6944.0 29 7700.5 30 8888.0 31 10376.0 32 12062.0 33 13798.5 34 15952.5 35 17388.0 36 19063.5 37 21069.0 38 21721.5 39 21620.5 40 21717.0 41 22645.5 42 23687.0 43 25075.5 44 26509.0 45 27403.5 46 27157.0 47 28512.0 48 33462.5 49 32649.5 50 27378.5 51 24807.5 52 22937.5 53 22151.5 54 21478.0 55 19900.0 56 18150.0 57 16244.5 58 14649.5 59 13149.0 60 11417.0 61 9435.5 62 7451.5 63 5695.5 64 4079.0 65 3040.5 66 2199.0 67 1609.0 68 1126.0 69 616.5 70 398.5 71 282.0 72 189.5 73 133.0 74 90.5 75 70.5 76 54.0 77 23.5 78 9.5 79 6.5 80 6.0 81 3.5 82 3.0 83 3.0 84 1.0 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017377531515015646 2 6.632645616418186E-4 3 0.0017244878602687285 4 0.0 5 0.0017244878602687285 6 5.306116493134548E-4 7 0.0 8 0.0 9 0.0 10-11 1.326529123283637E-4 12-13 5.969381054776367E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.001989793684925456 22-23 0.0 24-25 0.003780608001358366 26-27 0.002653058246567274 28-29 0.004443872563000184 30-31 0.013464270601328918 32-33 9.285703862985461E-4 34-35 0.0027193847027314564 36-37 0.004244893194507639 38-39 0.002653058246567274 40-41 0.0041785667383434566 42-43 0.0020561201410896376 44-45 6.632645616418186E-4 46-47 6.632645616418186E-5 48-49 1.989793684925456E-4 50-51 1.989793684925456E-4 52-53 8.622439301343642E-4 54-55 0.00391326091368673 56-57 3.316322808209093E-4 58-59 9.94896842462728E-4 60-61 8.622439301343642E-4 62-63 0.0 64-65 6.632645616418186E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.306116493134548E-4 76-77 6.632645616418186E-4 78-79 1.989793684925456E-4 80-81 0.007693868915045096 82-83 0.001923467228761274 84-85 0.00391326091368673 86-87 0.001923467228761274 88-89 0.0014591820356120008 90-91 1.989793684925456E-4 92-93 0.0 94-95 1.326529123283637E-4 96-97 0.0017244878602687285 98-99 0.004045913826015093 100-101 7.959174739701824E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 753847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.15752150833914 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14245994726731 44.44444396711532 2 11.7851419300568 13.236487228319882 3 3.75569369490368 6.327313483508612 4 1.646870697544262 3.699367064751814 5 0.888325683218814 2.49430843308853 6 0.5561764437434367 1.874009436117217 7 0.37377635158539546 1.4693247452425764 8 0.27760246976993974 1.2471573333498764 9 0.20120975417987963 1.01694969882398 >10 1.2368526428616011 12.861633611383258 >50 0.0945326870519874 3.5994398440187196 >100 0.03734009288663006 3.7927836573501663 >500 0.0018906376142623083 0.7239707064610307 >1k 0.0016543079124795197 1.610121468521581 >5k 4.7265940356557706E-4 1.6026893219474623 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6796 0.9015091921835598 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5280 0.7004073770937604 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3669 0.48670353533276645 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2648 0.35126491184550707 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1416 0.187836523856963 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1201 0.15931614770636482 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1124 0.1491018734570808 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1039 0.1378263759091699 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1035 0.13729576425985643 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 962 0.12761210165988587 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.632645616418186E-5 0.0 18-19 0.0 0.0 0.0 1.326529123283637E-4 0.0 20-21 0.0 0.0 0.0 2.653058246567274E-4 0.0 22-23 0.0 0.0 0.0 7.295910178060004E-4 0.0 24-25 0.0 0.0 0.0 0.0014591820356120008 0.0 26-27 0.0 0.0 0.0 0.001857140772597092 0.0 28-29 0.0 0.0 0.0 0.0041785667383434566 0.0 30-31 0.0 0.0 0.0 0.009086724494492915 0.0 32-33 0.0 0.0 0.0 0.014193861619134917 0.0 34-35 0.0 0.0 0.0 0.01896936646295601 0.0 36-37 0.0 0.0 0.0 0.024872421061568194 0.0 38-39 0.0 0.0 0.0 0.03342853390674766 0.0 40-41 0.0 0.0 0.0 0.05113769770258421 0.0 42-43 0.0 0.0 0.0 0.08231113209974969 0.0 44-45 0.0 0.0 0.0 0.11275497547910915 0.0 46-47 0.0 0.0 0.0 0.18524979206655992 0.0 48-49 0.0 0.0 0.0 0.2504486984759507 0.0 50-51 0.0 0.0 0.0 0.296877217790878 0.0 52-53 0.0 0.0 0.0 0.3620097977441046 0.0 54-55 0.0 0.0 0.0 0.4240913607137788 0.0 56-57 0.0 0.0 0.0 0.5558157026558439 0.0 58-59 0.0 0.0 0.0 0.7302542823676422 0.0 60-61 0.0 0.0 0.0 0.9122540780821573 0.0 62-63 0.0 0.0 0.0 1.0959120352007767 0.0 64-65 0.0 0.0 0.0 1.3097485298740992 0.0 66-67 0.0 0.0 0.0 1.5720696640034384 0.0 68-69 0.0 0.0 0.0 1.8672887203902118 0.0 70-71 0.0 0.0 0.0 2.118997621533282 0.0 72-73 0.0 0.0 0.0 2.3340943188737238 0.0 74-75 0.0 0.0 0.0 2.5677624239401364 0.0 76-77 0.0 0.0 0.0 2.8478590483214763 0.0 78-79 0.0 0.0 0.0 3.1230475149466668 0.0 80-81 0.0 0.0 0.0 3.4620420324018006 0.0 82-83 0.0 0.0 0.0 3.7871079940624557 0.0 84-85 0.0 0.0 0.0 4.249602372895296 0.0 86-87 0.0 0.0 0.0 4.751892625426645 0.0 88-89 0.0 0.0 0.0 5.402621486853433 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGGA 25 0.00502058 57.00008 2 CGCAGTA 50 0.0016159787 37.99753 8 GTATCAA 8995 0.0 25.248535 1 GAGTACT 5020 0.0 23.606533 12-13 CCCTATA 125 2.2548811E-4 22.800034 2 AGTACTT 5115 0.0 22.610947 12-13 GTACATG 4345 0.0 22.307446 1 GGTATCA 6635 0.0 22.270447 1 GTACTTT 5435 0.0 21.892855 14-15 GGTATGC 175 4.0484665E-6 21.712875 8 ACCGTGC 110 0.0028775411 21.589508 8 ATCAACG 10565 0.0 21.354506 3 TCAACGC 10600 0.0 21.328802 4 TACATGG 4590 0.0 21.214626 2 CAACGCA 10650 0.0 21.18407 5 TATCAAC 10655 0.0 21.130955 2 GTATGGT 205 6.8053487E-7 20.859222 1 AACGCAG 10870 0.0 20.842712 6 AGAGTAC 8100 0.0 20.78723 10-11 ACATGGG 4460 0.0 20.660093 3 >>END_MODULE