FastQCFastQC Report
Thu 26 May 2016
SRR937122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474739
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41920.8830115073756316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.6112832524818901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.4118052235017557No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12960.2729921072420846No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9390.19779289251567705No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7660.16135181647178765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7080.14913457710447214No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.13691733773715664No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.12154046749898366No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5700.1200659730925835No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC1004.9242484E-528.5193274
GTATCAA54200.028.1480271
GTCTAGA1658.342067E-825.9239271
GTACATG29150.025.2717531
CAACGCA62700.024.2614215
TCAACGC62950.024.1625234
ATCAACG63400.023.9910223
TATCAAC63650.023.968942
AACGCAG63800.023.8431226
CTAATAC1401.7673257E-523.7661063
GGTATCA38400.023.76361
GAGTACT28800.023.75984212-13
TACATGG30050.023.407742
GACTCAC1050.002184365422.6367747
ACATGGG30350.022.3957393
AGTACTT29400.022.14352812-13
GTACTTT31250.021.74500714-15
AGAGTAC47500.021.71471610-11
GTACACC1100.002863614121.605554
ACGCAGA70200.021.4662387
Produced by FastQC (version 0.11.5)