##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937122_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 474739 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74375814921462 33.0 31.0 34.0 30.0 34.0 2 31.82859213167656 33.0 31.0 34.0 30.0 34.0 3 31.820931080025023 33.0 31.0 34.0 30.0 34.0 4 35.41899022410209 37.0 35.0 37.0 33.0 37.0 5 35.429254811591214 37.0 35.0 37.0 33.0 37.0 6 35.50545457609339 37.0 35.0 37.0 33.0 37.0 7 35.447174131470135 37.0 35.0 37.0 33.0 37.0 8 35.402732870061236 37.0 35.0 37.0 33.0 37.0 9 37.01809204636653 39.0 37.0 39.0 33.0 39.0 10-11 36.850840145848565 39.0 37.0 39.0 33.0 39.0 12-13 36.799821586176826 39.0 37.0 39.0 32.5 39.0 14-15 37.8985948068307 40.0 38.0 41.0 33.0 41.0 16-17 37.853235146048675 40.0 38.0 41.0 33.0 41.0 18-19 37.8712840107933 40.0 38.0 41.0 33.0 41.0 20-21 37.83552225538664 40.0 38.0 41.0 32.5 41.0 22-23 37.736004415057536 40.0 37.0 41.0 32.0 41.0 24-25 37.56923488485252 40.0 37.0 41.0 32.0 41.0 26-27 37.608003555637936 40.0 37.0 41.0 32.0 41.0 28-29 37.533813316369624 40.0 37.0 41.0 32.0 41.0 30-31 37.33251534000787 40.0 37.0 41.0 32.0 41.0 32-33 37.07813451180543 39.0 36.0 41.0 31.0 41.0 34-35 36.87381803475173 39.0 35.5 41.0 31.0 41.0 36-37 36.65597412472959 39.0 35.0 41.0 30.0 41.0 38-39 36.4851023404439 39.0 35.0 41.0 30.0 41.0 40-41 36.27618965368339 39.0 35.0 41.0 30.0 41.0 42-43 35.99786724916217 39.0 35.0 40.5 30.0 41.0 44-45 35.64629617537216 39.0 35.0 40.0 28.0 41.0 46-47 35.14752105893975 38.0 35.0 40.0 25.5 41.0 48-49 34.70127796536623 38.0 34.0 40.0 24.0 41.0 50-51 33.99945654349021 38.0 33.5 39.5 23.0 40.5 52-53 34.19669966023436 38.0 34.0 40.0 23.0 40.5 54-55 34.583103557955 39.0 35.0 40.0 23.0 41.0 56-57 34.4637337568643 38.5 34.0 41.0 22.5 41.0 58-59 34.21961330330982 38.0 34.0 40.0 21.5 41.0 60-61 33.92868502482417 38.0 34.0 40.0 20.5 41.0 62-63 33.624170333593824 37.5 34.0 40.0 19.0 41.0 64-65 33.30839471793975 37.0 33.0 40.0 18.0 41.0 66-67 32.912210709463515 36.5 33.0 39.5 13.5 41.0 68-69 32.520010995515435 36.0 32.5 39.0 10.0 41.0 70-71 32.095417692669024 35.5 32.0 39.0 7.5 40.5 72-73 31.697560133041524 35.0 31.5 38.5 6.5 40.0 74-75 31.27097731595677 35.0 31.0 37.0 2.0 39.5 76-77 30.852396790657604 35.0 31.0 37.0 2.0 39.0 78-79 30.40883306406257 35.0 31.0 36.0 2.0 39.0 80-81 29.968074036470565 35.0 30.0 36.0 2.0 37.5 82-83 29.558404723437512 34.0 30.0 35.5 2.0 37.0 84-85 29.221650212011234 34.0 29.0 35.0 2.0 37.0 86-87 28.90154906169495 34.0 29.0 35.0 2.0 36.0 88-89 28.534673789176793 34.0 29.0 35.0 2.0 36.0 90-91 28.175538559081936 34.0 29.0 35.0 2.0 35.5 92-93 27.80974598674219 34.0 27.5 35.0 2.0 35.0 94-95 27.38348439879597 34.0 27.0 35.0 2.0 35.0 96-97 26.89785650641721 34.0 25.5 35.0 2.0 35.0 98-99 26.31462656322737 33.5 25.0 35.0 2.0 35.0 100-101 24.357628086169452 31.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4542217478905215 1101 2 0.4866205394668164 1101 3 0.4180779784637032 1101 4 0.40087729998910504 1101 5 0.2993593980857554 1101 6 0.26488541591980663 1101 7 0.43975819494096413 1101 8 0.375162255668009 1101 9 0.5403302721723762 1101 10-11 0.5483395357555665 1101 12-13 0.535512402068349 1101 14-15 1.0211711016544314 1101 16-17 0.9208633727517181 1101 18-19 0.8709143035487514 1101 20-21 0.7881716165978077 1101 22-23 0.8772983545679551 1101 24-25 0.7859542775263648 1101 26-27 0.9142425704854773 1101 28-29 1.0032225000716437 1101 30-31 0.9408240058236075 1101 32-33 0.99390238043366 1101 34-35 1.1243225430898107 1101 36-37 1.2073839976850067 1101 38-39 1.1763677169272952 1101 40-41 1.354473775769911 1101 42-43 1.2559815640589704 1101 44-45 1.395945619332167 1101 46-47 1.3578016552996104 1101 48-49 1.4918123160082075 1101 50-51 1.46537892343094 1101 52-53 1.5055091557691966 1101 54-55 1.6204428346520388 1101 56-57 1.7133336896878433 1101 58-59 1.4592187912671406 1101 60-61 1.6710441463457357 1101 62-63 1.8762173547193584 1101 64-65 1.9073754989796328 1101 66-67 1.8904384174061448 1101 68-69 1.7668828862379193 1101 70-71 1.8346500483180108 1101 72-73 1.9231424491240645 1101 74-75 1.8420582477924725 1101 76-77 1.8546912107150035 1101 78-79 1.8017621756992526 1101 80-81 1.7523015812362601 1101 82-83 1.8510928186777384 1101 84-85 1.7795085974829767 1101 86-87 1.9234797673085957 1101 88-89 2.087117871171948 1101 90-91 2.1655475003474685 1101 92-93 2.384512477414358 1101 94-95 2.43192142406971 1101 96-97 2.5438027097959868 1101 98-99 2.464022686070198 1101 100-101 2.31880234709946 1102 1 0.3555267474062127 1102 2 0.3465876942154509 1102 3 0.34706339291746957 1102 4 0.47042856993800086 1102 5 0.24441939457248907 1102 6 0.3226776261994573 1102 7 0.4356555483316811 1102 8 0.40919993960169165 1102 9 0.46838209784191065 1102 10-11 0.5999520559144642 1102 12-13 0.6415945742985159 1102 14-15 0.651174707955775 1102 16-17 0.752860076187325 1102 18-19 0.6563560645182775 1102 20-21 0.6219714707040396 1102 22-23 0.6114841212243931 1102 24-25 0.6463518353106394 1102 26-27 0.7690432470910835 1102 28-29 0.784960824876265 1102 30-31 0.8118730126679239 1102 32-33 0.962345601982598 1102 34-35 0.623218489530494 1102 36-37 0.8211671829082547 1102 38-39 1.0667791143718048 1102 40-41 0.9333644906110763 1102 42-43 0.8211084111939257 1102 44-45 0.9502562986356651 1102 46-47 0.9264300076139023 1102 48-49 1.2503313136643825 1102 50-51 1.1975182919287164 1102 52-53 1.2593013813715288 1102 54-55 1.3398742320199233 1102 56-57 1.4194890824217197 1102 58-59 1.3434570654522133 1102 60-61 1.2323772629726477 1102 62-63 1.4370828560964881 1102 64-65 1.337648018252004 1102 66-67 1.1882505033860582 1102 68-69 1.26716307232088 1102 70-71 1.4063352745764774 1102 72-73 1.3515414975858562 1102 74-75 1.400657908395189 1102 76-77 1.4081276766053605 1102 78-79 1.4623547692647136 1102 80-81 1.6267180198413058 1102 82-83 1.4317946191469417 1102 84-85 1.4220557297620005 1102 86-87 1.4855582226952535 1102 88-89 1.6270112800679684 1102 90-91 1.7019752481821833 1102 92-93 1.7176706944458395 1102 94-95 1.5961441238486813 1102 96-97 1.729579216521273 1102 98-99 1.7670269703999715 1102 100-101 1.3685479633091013 1103 1 0.5573728783475183 1103 2 0.5775729531718277 1103 3 0.5381147269136797 1103 4 0.4480803892170968 1103 5 0.4250951040195545 1103 6 0.44747313564900537 1103 7 0.44890600428135485 1103 8 0.6863056827066103 1103 9 0.48665198814812527 1103 10-11 0.56534551966093 1103 12-13 0.5955627101068259 1103 14-15 0.8733077194214189 1103 16-17 0.6495389546840542 1103 18-19 0.685074247738271 1103 20-21 0.7685249519618083 1103 22-23 0.8912976471111591 1103 24-25 1.0540386417487042 1103 26-27 1.1249148509527913 1103 28-29 0.9501386762676844 1103 30-31 1.0469449809348816 1103 32-33 1.1695997657912685 1103 34-35 1.0883748948923468 1103 36-37 0.9933832312734836 1103 38-39 1.0872011403303645 1103 40-41 1.0937440144383217 1103 42-43 1.185483986115898 1103 44-45 1.2732804723874978 1103 46-47 1.500624407896069 1103 48-49 1.5392487677193643 1103 50-51 1.6366350522666764 1103 52-53 1.676696504083587 1103 54-55 1.9426525562874417 1103 56-57 1.977740752464804 1103 58-59 2.018033506368397 1103 60-61 1.988842088825571 1103 62-63 2.0477731701124355 1103 64-65 1.890349863496752 1103 66-67 1.900406680108432 1103 68-69 2.0435820107601437 1103 70-71 1.8800279972828662 1103 72-73 1.9619690534297263 1103 74-75 2.162300355227643 1103 76-77 2.3243025025114576 1103 78-79 2.28037634645181 1103 80-81 2.0845876492503734 1103 82-83 1.9994253823242865 1103 84-85 2.017521135189444 1103 86-87 2.1715661949465748 1103 88-89 2.0412510892160256 1103 90-91 2.1595807901198043 1103 92-93 1.9851629446306127 1103 94-95 1.8274094218687331 1103 96-97 2.122448323072593 1103 98-99 1.9541502248083695 1103 100-101 2.1870999309380004 1104 1 0.44418678289286007 1104 2 0.15488839034115642 1104 3 0.25813525405339277 1104 4 0.3410558914235864 1104 5 0.22914558557795317 1104 6 0.23362284553900992 1104 7 0.5305722274604392 1104 8 0.3308302236969638 1104 9 0.6320491053636275 1104 10-11 0.5826216649820211 1104 12-13 0.6425349569992491 1104 14-15 0.8954694276664839 1104 16-17 0.7684754138561232 1104 18-19 0.8174120954296598 1104 20-21 0.7233583330996467 1104 22-23 0.6184015925379995 1104 24-25 0.8578187110709905 1104 26-27 0.7537546183118735 1104 28-29 0.5508547071344196 1104 30-31 0.6101791112787609 1104 32-33 0.9303678747717257 1104 34-35 0.9109533662770346 1104 36-37 0.7549591212148385 1104 38-39 0.8926641586492821 1104 40-41 0.7011788822387004 1104 42-43 0.9130786476772741 1104 44-45 1.0697697785770899 1104 46-47 0.9949189688951989 1104 48-49 1.063512434336502 1104 50-51 1.2467671309333141 1104 52-53 1.1123012426044525 1104 54-55 1.415886928533638 1104 56-57 1.5241652633901523 1104 58-59 1.4022478249122798 1104 60-61 1.5674802133223267 1104 62-63 1.5458802844596704 1104 64-65 1.6587361676785548 1104 66-67 1.391565150318165 1104 68-69 1.3651946007391693 1104 70-71 1.2575675396727917 1104 72-73 1.2019741679359157 1104 74-75 1.4531913505865788 1104 76-77 1.4502394420324194 1104 78-79 1.269983186067119 1104 80-81 1.4923079617881712 1104 82-83 1.6503871168330342 1104 84-85 1.5316871078268335 1104 86-87 1.5085827790784734 1104 88-89 1.6522635195395203 1104 90-91 1.9721298185364162 1104 92-93 1.82306765585108 1104 94-95 1.8380824977949608 1104 96-97 1.959324949433725 1104 98-99 1.763284974767764 1104 100-101 1.9137045208617316 1105 1 0.2776224860547032 1105 2 0.07528957439682316 1105 3 0.09928634003216175 1105 4 0.06312775108050062 1105 5 -0.012523821958311032 1105 6 -0.04276776602495147 1105 7 0.31979605281618717 1105 8 0.23602651619199833 1105 9 0.3588088427156677 1105 10-11 0.39565737289910174 1105 12-13 0.18399671889627456 1105 14-15 0.2511834473135721 1105 16-17 0.37145417773053424 1105 18-19 0.1836015205603303 1105 20-21 0.33417730493671627 1105 22-23 0.3642476890773736 1105 24-25 0.2304752675613031 1105 26-27 0.20415148302122788 1105 28-29 0.19297705162804846 1105 30-31 0.4236229776088649 1105 32-33 0.3316688301794244 1105 34-35 0.32706096158995024 1105 36-37 0.2332970050521581 1105 38-39 0.3427975036065405 1105 40-41 0.2783862068990075 1105 42-43 0.08161240792581737 1105 44-45 0.7141706773181369 1105 46-47 0.8574805393936629 1105 48-49 1.2104930527559432 1105 50-51 1.075756681285398 1105 52-53 0.8223700747562432 1105 54-55 1.2202720557165918 1105 56-57 1.4986117728431836 1105 58-59 1.2994117183472724 1105 60-61 1.4570376982739646 1105 62-63 1.4965287808119996 1105 64-65 1.5640156769374016 1105 66-67 1.6730775555600275 1105 68-69 1.6033173890555297 1105 70-71 1.4598120146740783 1105 72-73 1.473946365611397 1105 74-75 1.7208679781677816 1105 76-77 1.6874131629709481 1105 78-79 1.6980522005032448 1105 80-81 1.2860710634750916 1105 82-83 1.4260327402256614 1105 84-85 1.5707752106034967 1105 86-87 1.3325991371166168 1105 88-89 1.3920129974533957 1105 90-91 1.536396936422868 1105 92-93 1.3597543605861127 1105 94-95 1.4780550758899302 1105 96-97 1.7499317581500868 1105 98-99 1.7508500126313926 1105 100-101 1.9727190758699926 1106 1 -0.02708108943732057 1106 2 -0.06890821946569403 1106 3 0.14357047504713094 1106 4 0.10220720914855264 1106 5 0.24144827451783613 1106 6 -0.06822630209860847 1106 7 0.15837844406264878 1106 8 0.1291410110155624 1106 9 0.07313259782956294 1106 10-11 0.21829892800815998 1106 12-13 0.8345692333604333 1106 14-15 0.8998475802305279 1106 16-17 0.6982029895310333 1106 18-19 0.25905723239067413 1106 20-21 0.2601861649914241 1106 22-23 -0.11634975942053671 1106 24-25 0.16209630252544116 1106 26-27 0.059743888227522746 1106 28-29 0.44058249676913874 1106 30-31 0.5514926105379274 1106 32-33 0.6356623102120196 1106 34-35 0.39449985706873747 1106 36-37 0.15244295387687856 1106 38-39 0.04187437950928796 1106 40-41 0.5643489107488548 1106 42-43 0.43186699328654754 1106 44-45 0.614083529239025 1106 46-47 0.9364544514548001 1106 48-49 1.4229649111887284 1106 50-51 1.0392876309573822 1106 52-53 1.1233335451864193 1106 54-55 1.2373829355087338 1106 56-57 1.114595376093483 1106 58-59 1.5807063514780708 1106 60-61 1.873670070557651 1106 62-63 1.5286484964510478 1106 64-65 1.4424602451106026 1106 66-67 1.8413701794434445 1106 68-69 1.7315178619145506 1106 70-71 1.1584854272476122 1106 72-73 1.6653897153253396 1106 74-75 1.685589144660966 1106 76-77 1.727852292458465 1106 78-79 1.708961848925295 1106 80-81 1.711761187499718 1106 82-83 1.829875324973134 1106 84-85 1.6499266465294902 1106 86-87 2.059914411431336 1106 88-89 2.013507909780813 1106 90-91 1.9088137624575516 1106 92-93 1.9070281588705846 1106 94-95 1.9495181146143423 1106 96-97 1.9507959766012704 1106 98-99 2.1303677932603122 1106 100-101 2.2310122646020716 1107 1 0.2920964729182174 1107 2 0.16665714891783523 1107 3 0.22403183128728443 1107 4 0.2843693277994461 1107 5 0.1687375191217413 1107 6 0.01544338891230268 1107 7 0.26408100038099747 1107 8 0.4259177270146921 1107 9 0.42263409850227873 1107 10-11 0.5168120545821395 1107 12-13 0.6065877244081435 1107 14-15 0.30346529391827204 1107 16-17 0.29616644441311024 1107 18-19 0.4083827921243568 1107 20-21 0.35935130798656445 1107 22-23 0.18014522108796882 1107 24-25 0.5817441906592009 1107 26-27 0.49028746523330113 1107 28-29 0.8357021481070959 1107 30-31 0.5407694127826161 1107 32-33 0.14553455179157737 1107 34-35 0.53769623245541 1107 36-37 0.8394750717228092 1107 38-39 0.30605027445070476 1107 40-41 0.6713221516269456 1107 42-43 0.5718665333102422 1107 44-45 0.5423272246887123 1107 46-47 0.3228460522873604 1107 48-49 0.6709898879024294 1107 50-51 0.34614932758000805 1107 52-53 0.5829188765418465 1107 54-55 0.9430555933668501 1107 56-57 0.8761494060609394 1107 58-59 0.816449960682192 1107 60-61 0.7714230862773377 1107 62-63 0.6494555248890777 1107 64-65 1.1399338252212274 1107 66-67 0.7607478314943705 1107 68-69 0.8748004828595803 1107 70-71 0.4829584668860676 1107 72-73 0.5604921175516253 1107 74-75 0.8312118950893144 1107 76-77 0.9066910822607923 1107 78-79 1.0091193908118932 1107 80-81 0.9301464406591826 1107 82-83 0.7099537509341971 1107 84-85 0.9414251343573632 1107 86-87 0.7148861803852355 1107 88-89 0.8296956261134234 1107 90-91 0.8533636181399835 1107 92-93 0.7756876729056152 1107 94-95 0.5642132078170405 1107 96-97 0.5863049978866748 1107 98-99 0.7025380764907219 1107 100-101 1.057100971033691 1108 1 -0.6413420671045955 1108 2 -0.7772656022891766 1108 3 -0.4507701249321485 1108 4 -0.8287226551383213 1108 5 0.011224355264928931 1108 6 -0.44012761779071496 1108 7 -0.20627102536860065 1108 8 -0.4768025429810905 1108 9 -0.32357299201193257 1108 10-11 -0.5491166027825827 1108 12-13 -0.7513207457720128 1108 14-15 -0.7024330514200798 1108 16-17 -0.49651648241616186 1108 18-19 -0.5894531791885953 1108 20-21 -0.5546031614748514 1108 22-23 -0.46850089381648274 1108 24-25 -0.48052827383354213 1108 26-27 -0.34236358709400605 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-1.826694519732051 2316 88-89 -1.5106891045152082 2316 90-91 -0.9267089790057845 2316 92-93 -1.0480930289238692 2316 94-95 -1.5688202057041778 2316 96-97 -1.5204376332461997 2316 98-99 -1.5124667604653332 2316 100-101 -1.729229705540888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1110.0 3 368.0 4 377.0 5 416.0 6 404.0 7 486.0 8 574.0 9 840.0 10 1142.0 11 1379.0 12 1957.0 13 2541.0 14 2810.0 15 3698.0 16 4964.0 17 6686.0 18 5751.0 19 3342.0 20 3115.0 21 3201.0 22 3418.0 23 3753.0 24 4037.0 25 4622.0 26 5254.0 27 6066.0 28 7292.0 29 8762.0 30 10567.0 31 13131.0 32 16495.0 33 21412.0 34 29066.0 35 41365.0 36 61233.0 37 90143.0 38 86219.0 39 16737.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12150814739269 19.37652901896247 15.240144215066723 21.261818618578122 2 14.841223185144447 21.404686502443127 40.93578341203168 22.818306900380744 3 18.536372619456458 26.993925782031862 31.04235097307581 23.427350625435867 4 12.034416560071985 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29.549315563363443 21.561465538033445 50-51 20.93616761320548 27.648763882101147 29.712436171826283 21.702632332867086 52-53 21.28007600365694 28.15905998137826 28.93057714750355 21.63028686746125 54-55 20.900730666616106 27.768491279293283 29.169365719865137 22.16141233422547 56-57 21.23339980259367 28.036544399043095 28.81763721267319 21.912418585690045 58-59 21.62182089357301 27.39155805443848 29.044809258880854 21.941811793107664 60-61 21.444387560544484 27.31406865254682 29.69360646032559 21.54793732658311 62-63 21.13204804504934 27.470640716525573 29.723634578699944 21.673676659725142 64-65 20.931849278635237 27.613571369134206 29.49068919643431 21.96389015579625 66-67 21.053923324218303 27.67973388051589 29.15885428736354 22.107488507902264 68-69 20.865616266489972 27.430002875836546 29.519145381438104 22.18523547623537 70-71 21.160954593097056 27.507703003776445 29.2643565555854 22.066985847541094 72-73 21.0120645902748 27.43348569008761 29.37616598213223 22.178283737505357 74-75 20.80034971848715 28.18141119836517 28.92751241658635 22.09072666656133 76-77 20.833390395626044 27.816866911420007 28.900242822002518 22.44949987095143 78-79 20.949446974214606 27.77339955454505 28.781467894161256 22.49568557707909 80-81 21.006365373715493 27.8330759230424 28.626061158999494 22.534497544242612 82-83 20.955274638561388 27.520430513077955 29.055418655636945 22.468876192723712 84-85 20.968671292429008 27.606573540456807 28.457602855093274 22.967152312020907 86-87 21.1746673829915 27.56428488659946 28.531007384466285 22.730040345942758 88-89 21.23150011060432 27.360243540180967 28.31786628464285 23.090390064571856 90-91 21.187009323447032 27.34337061939669 28.600787715929282 22.868832341226998 92-93 21.289170525118124 27.6211395732263 28.41042334982064 22.679266551834935 94-95 21.153894771437358 27.465122550362153 28.363656665163845 23.017326013036644 96-97 21.147593490487118 27.671962020115178 28.236160542136307 22.944283947261397 98-99 21.433306179399334 28.002099248506973 27.51891378643519 23.045680785658508 100-101 21.693464074330134 27.61759523126992 27.581867696406594 23.107072997993356 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 433.0 1 291.0 2 194.0 3 244.5 4 232.5 5 232.0 6 336.0 7 683.0 8 1195.5 9 1425.0 10 1206.0 11 1038.5 12 1087.0 13 1076.5 14 1122.0 15 1194.0 16 1237.0 17 1280.5 18 1305.0 19 1348.5 20 1469.0 21 1668.0 22 1865.5 23 2166.0 24 2501.5 25 2928.0 26 3596.0 27 4209.5 28 4623.5 29 5081.5 30 5759.5 31 6791.5 32 7952.5 33 9053.0 34 10336.5 35 11228.0 36 12148.5 37 13522.5 38 14095.0 39 13983.0 40 14061.5 41 14597.5 42 15198.0 43 15707.0 44 16518.0 45 17170.5 46 17125.0 47 17978.5 48 20743.5 49 20404.5 50 17253.0 51 15519.5 52 14487.0 53 14140.5 54 13674.5 55 12472.5 56 11130.0 57 9899.0 58 8865.5 59 7795.0 60 6689.0 61 5529.0 62 4357.5 63 3296.0 64 2419.0 65 1808.0 66 1259.5 67 922.5 68 648.0 69 360.0 70 236.5 71 159.5 72 102.0 73 81.0 74 64.0 75 43.5 76 27.0 77 15.5 78 12.0 79 8.5 80 7.5 81 6.0 82 4.0 83 3.5 84 2.5 85 3.0 86 2.0 87 0.5 88 0.5 89 1.0 90 2.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035809149869717886 2 0.029911172244117292 3 0.023170626386288043 4 0.042128411611432805 5 0.06487775388160652 6 0.06719481652023533 7 0.06508839593966369 8 0.07119701562332144 9 0.06824802681052115 10-11 0.07456728855223607 12-13 0.07182894179749294 14-15 0.06424582770743503 16-17 0.05508289818194839 18-19 0.044340153221033025 20-21 0.052555193485262434 22-23 0.02801539372160282 24-25 0.016640722586515957 26-27 0.011479992164115441 28-29 0.012743844512458425 30-31 0.008215040264229399 32-33 0.005371372480457683 34-35 0.009057608496458053 36-37 0.004950088364343355 38-39 0.005371372480457683 40-41 0.006003298654629175 42-43 0.0032649518998860427 44-45 0.0011585313193144021 46-47 0.0028436677837717144 48-49 0.0011585313193144021 50-51 0.005687335567543429 52-53 0.005266051451429101 54-55 0.007477793061029323 56-57 0.0175886118477732 58-59 0.008004398206172235 60-61 0.018536501109030434 62-63 0.01253320245440126 64-65 0.010216139815772456 66-67 0.013691733773715662 68-69 0.019905674486402 70-71 0.01822053802194469 72-73 0.018009895963887527 74-75 0.01506090715108723 76-77 0.02348658947337379 78-79 0.03538786575360356 80-81 0.029489888128002968 82-83 0.030016493273145876 84-85 0.016535401557487377 86-87 0.01980035345737342 88-89 0.01558751229623014 90-91 0.015903475383315886 92-93 0.02769943063451707 94-95 0.01621943847040163 96-97 0.036230433985832214 98-99 0.059717023459206 100-101 0.06635224828800668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 474739.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.26962741497856 #Duplication Level Percentage of deduplicated Percentage of total 1 83.3120048869357 52.71119508391294 2 9.777995412619036 12.373002532435518 3 2.766394402164794 5.250862293235467 4 1.254399831095743 3.1746163977135873 5 0.6693928759579254 2.1176118928049448 6 0.4345202441266929 1.6495160370096826 7 0.3212723157188016 1.4228745799993152 8 0.22970027960400682 1.1626440886129528 9 0.17386199576287245 0.9900165324187367 >10 0.9792252427957113 11.17651212850558 >50 0.05792810384606742 2.495631084058061 >100 0.019975208033735016 2.4096910358112984 >500 0.001997520803373501 0.8867831521648221 >1k 0.001331680535582334 2.1790431613171224 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4192 0.8830115073756316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 0.6112832524818901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1955 0.4118052235017557 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1296 0.2729921072420846 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 939 0.19779289251567705 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 766 0.16135181647178765 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 708 0.14913457710447214 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 650 0.13691733773715664 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 577 0.12154046749898366 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 570 0.1200659730925835 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0532102902858202E-4 0.0 12-13 0.0 0.0 0.0 2.1064205805716404E-4 0.0 14-15 0.0 0.0 0.0 4.212841161143281E-4 0.0 16-17 0.0 0.0 0.0 4.212841161143281E-4 0.0 18-19 0.0 0.0 0.0 6.319261741714921E-4 0.0 20-21 0.0 0.0 0.0 9.478892612572382E-4 0.0 22-23 0.0 0.0 0.0 0.0015798154354287304 0.0 24-25 0.0 0.0 0.0 0.0035809149869717887 0.0 26-27 0.0 0.0 0.0 0.005687335567543429 0.0 28-29 0.0 0.0 0.0 0.009689534670629545 0.0 30-31 0.0 0.0 0.0 0.015692833325258723 0.0 32-33 0.0 0.0 0.0 0.023275947415316627 0.0 34-35 0.0 0.0 0.0 0.028963282982860057 0.0 36-37 0.0 0.0 0.0 0.03443997649234633 0.0 38-39 0.0 0.0 0.0 0.04697317894674759 0.0 40-41 0.0 0.0 0.0 0.07298747311680734 0.0 42-43 0.0 0.0 0.0 0.11806487354104045 0.0 44-45 0.0 0.0 0.0 0.16093053235567334 0.0 46-47 0.0 0.0 0.0 0.26182807816505493 0.0 48-49 0.0 0.0 0.0 0.3571436094359216 0.0 50-51 0.0 0.0 0.0 0.4236011787529569 0.0 52-53 0.0 0.0 0.0 0.5122814851950229 0.0 54-55 0.0 0.0 0.0 0.5916935410825738 0.0 56-57 0.0 0.0 0.0 0.7845363452339075 0.0 58-59 0.0 0.0 0.0 1.0471016706021623 0.0 60-61 0.0 0.0 0.0 1.3242012979763618 0.0 62-63 0.0 0.0 0.0 1.589189007012274 0.0 64-65 0.0 0.0 0.0 1.9163161231750498 0.0 66-67 0.0 0.0 0.0 2.31516686010629 0.0 68-69 0.0 0.0 0.0 2.7595162815778775 0.0 70-71 0.0 0.0 0.0 3.130562266845572 0.0 72-73 0.0 0.0 0.0 3.4557936044858333 0.0 74-75 0.0 0.0 0.0 3.799561443235125 0.0 76-77 0.0 0.0 0.0 4.204626120879052 0.0 78-79 0.0 0.0 0.0 4.614851528945378 0.0 80-81 0.0 0.0 0.0 5.1101763284668 0.0 82-83 0.0 0.0 0.0 5.555473639199644 0.0 84-85 0.0 0.0 0.0 6.204145856986681 0.0 86-87 0.0 0.0 0.0 6.88831126155635 0.0 88-89 0.0 0.0 0.0 7.795757247666613 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACC 100 4.9242484E-5 28.519327 4 GTATCAA 5420 0.0 28.148027 1 GTCTAGA 165 8.342067E-8 25.923927 1 GTACATG 2915 0.0 25.271753 1 CAACGCA 6270 0.0 24.261421 5 TCAACGC 6295 0.0 24.162523 4 ATCAACG 6340 0.0 23.991022 3 TATCAAC 6365 0.0 23.96894 2 AACGCAG 6380 0.0 23.843122 6 CTAATAC 140 1.7673257E-5 23.766106 3 GGTATCA 3840 0.0 23.7636 1 GAGTACT 2880 0.0 23.759842 12-13 TACATGG 3005 0.0 23.40774 2 GACTCAC 105 0.0021843654 22.636774 7 ACATGGG 3035 0.0 22.395739 3 AGTACTT 2940 0.0 22.143528 12-13 GTACTTT 3125 0.0 21.745007 14-15 AGAGTAC 4750 0.0 21.714716 10-11 GTACACC 110 0.0028636141 21.60555 4 ACGCAGA 7020 0.0 21.466238 7 >>END_MODULE