FastQCFastQC Report
Thu 26 May 2016
SRR937122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474739
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47160.9933879457975856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36420.7671583754441914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.5164943263561662No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17750.3738896530514662No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9360.19716096634150557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8660.18241602227750406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.17567547641967482No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.1550325547300727No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.13101936011155604No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6090.1282810133568129No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5150.10848065989943949No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA300.003950823631.66631580-81
CGGTATC650.005828959429.2427652
TGCACCG856.4020103E-427.9408665
GTATCAA55800.027.3393631
GTACATG27100.026.129711
GATATAC1109.4320014E-525.9224591
CCTATAC1109.4455296E-525.9169943
TACATGG28300.024.8511852
ACATGGG27400.024.1040653
GAGTACT34500.024.09266512-13
GGTATCA40150.023.910211
AGTACTT34950.023.37476212-13
CAACGCA66450.022.945575
TCAACGC66700.022.8595664
CATGGGA17900.022.8209764
ATCAACG66950.022.7814043
GTACTTT36500.022.70865214-15
AACGCAG67350.022.707086
TATCAAC67600.022.6350272
GTCTAGA1050.002187767522.6307161
Produced by FastQC (version 0.11.5)