##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937122_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 474739 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03059997177397 31.0 31.0 34.0 30.0 34.0 2 32.085029879575934 33.0 31.0 34.0 30.0 34.0 3 32.20638287564325 33.0 31.0 34.0 30.0 34.0 4 35.938088086295835 37.0 35.0 37.0 35.0 37.0 5 35.841165356122 37.0 35.0 37.0 35.0 37.0 6 35.87747372766931 37.0 35.0 37.0 35.0 37.0 7 35.82514392118617 37.0 35.0 37.0 35.0 37.0 8 35.800593589319604 37.0 35.0 37.0 33.0 37.0 9 37.446561163081185 39.0 37.0 39.0 34.0 39.0 10-11 37.3545653506453 39.0 37.0 39.0 34.0 39.0 12-13 37.26385550797386 39.0 37.0 39.0 33.5 39.0 14-15 38.38538123052878 40.0 38.0 41.0 33.5 41.0 16-17 38.34708123832253 40.0 38.0 41.0 33.0 41.0 18-19 38.42084387421299 40.0 38.0 41.0 33.5 41.0 20-21 38.496374850180835 40.0 38.0 41.0 34.0 41.0 22-23 38.427584420070815 40.0 38.0 41.0 34.0 41.0 24-25 38.31460865865244 40.0 38.0 41.0 34.0 41.0 26-27 38.09796962120238 40.0 37.0 41.0 33.0 41.0 28-29 37.923725457567215 40.0 37.0 41.0 33.0 41.0 30-31 37.81320578254578 40.0 37.0 41.0 33.0 41.0 32-33 37.647627433179075 40.0 36.0 41.0 33.0 41.0 34-35 37.47449019355899 40.0 36.0 41.0 33.0 41.0 36-37 37.249478134301164 40.0 36.0 41.0 32.0 41.0 38-39 37.082339980494545 39.0 35.0 41.0 31.5 41.0 40-41 36.835925634927825 39.0 35.0 41.0 31.0 41.0 42-43 36.53153206288087 39.0 35.0 41.0 30.5 41.0 44-45 36.14225395427803 39.0 35.0 41.0 30.0 41.0 46-47 35.622072338695574 39.0 35.0 41.0 28.0 41.0 48-49 35.11382570212264 39.0 35.0 40.0 25.5 41.0 50-51 34.80923623296169 39.0 35.0 40.0 24.0 41.0 52-53 34.56796260682185 38.0 34.0 40.0 23.5 41.0 54-55 34.27261505795816 38.0 34.0 40.0 23.0 41.0 56-57 34.000319122717954 38.0 34.0 40.0 22.0 41.0 58-59 33.64615399198296 38.0 33.0 40.0 20.5 41.0 60-61 33.28621937527778 37.0 33.0 40.0 19.0 41.0 62-63 32.93773842047946 37.0 32.5 39.5 18.0 41.0 64-65 32.53037037193069 36.0 32.0 39.0 14.0 40.5 66-67 32.08778402448503 36.0 31.0 39.0 10.0 40.0 68-69 31.61736027585684 35.0 31.0 38.5 7.5 40.0 70-71 31.20814805608977 35.0 31.0 38.0 7.0 40.0 72-73 30.841592959499852 35.0 30.5 37.0 2.0 39.0 74-75 30.366543511276724 34.5 30.0 37.0 2.0 39.0 76-77 28.89097904322164 33.0 28.0 35.0 2.0 37.5 78-79 29.558306774880514 34.0 29.0 36.0 2.0 38.0 80-81 29.078578966547937 34.0 29.0 35.0 2.0 37.0 82-83 29.011557929725598 34.0 29.0 35.0 2.0 37.0 84-85 28.724473868799485 34.0 29.0 35.0 2.0 36.0 86-87 28.371044932057405 34.0 29.0 35.0 2.0 36.0 88-89 27.998209542506515 34.0 28.0 35.0 2.0 35.5 90-91 27.54185984298741 33.0 27.0 35.0 2.0 35.0 92-93 27.14178738211944 33.0 26.0 35.0 2.0 35.0 94-95 26.638480301807938 33.0 25.0 35.0 2.0 35.0 96-97 26.001614571375008 32.5 24.5 34.5 2.0 35.0 98-99 25.279139695706483 32.0 21.0 34.0 2.0 35.0 100-101 22.445673306806476 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47772301788761595 1101 2 0.39522729174844073 1101 3 0.4044345564873808 1101 4 0.3292571015554202 1101 5 0.4181324525928858 1101 6 0.43198527151095334 1101 7 0.4480453035961389 1101 8 0.41181718264254386 1101 9 0.44171058658990603 1101 10-11 0.5132118086296558 1101 12-13 0.5023270087987797 1101 14-15 0.7752743555623809 1101 16-17 0.7170151612728333 1101 18-19 0.6583170971273802 1101 20-21 0.4740587182406486 1101 22-23 0.5002733772217951 1101 24-25 0.5748858191032298 1101 26-27 0.5445276066077511 1101 28-29 0.5934933207238231 1101 30-31 0.7055193862928419 1101 32-33 0.9055393745239755 1101 34-35 1.0763580922271458 1101 36-37 1.1313687859250976 1101 38-39 1.12359079013018 1101 40-41 1.2029235768554685 1101 42-43 1.2767983558497562 1101 44-45 1.4411660154231427 1101 46-47 1.5560410982176691 1101 48-49 1.8672961126289351 1101 50-51 1.7802784798942852 1101 52-53 1.830828879291964 1101 54-55 1.838637258396389 1101 56-57 1.7882972159293402 1101 58-59 1.880370574760704 1101 60-61 1.6741261646580199 1101 62-63 1.7642682702667258 1101 64-65 1.9972185224951886 1101 66-67 1.8384561629587601 1101 68-69 1.9976581992387068 1101 70-71 1.9270024377684898 1101 72-73 1.9658877343245962 1101 74-75 2.147631458965506 1101 76-77 2.0572939317956624 1101 78-79 2.2721495628573827 1101 80-81 2.542888783310108 1101 82-83 2.185363406510657 1101 84-85 2.0143279226669257 1101 86-87 2.0302228832751155 1101 88-89 2.1327119353984223 1101 90-91 2.1136453185095583 1101 92-93 2.0706712010509243 1101 94-95 2.292728133673851 1101 96-97 2.360067984489266 1101 98-99 2.6087708239264344 1101 100-101 2.3457203186273823 1102 1 0.3592852960850301 1102 2 0.28881841816763654 1102 3 0.2880692266695064 1102 4 0.3076236714156053 1102 5 0.3946756239600546 1102 6 0.32347664402327325 1102 7 0.3460652307368193 1102 8 0.31768833972898136 1102 9 0.4741831685757987 1102 10-11 0.38584370267116697 1102 12-13 0.46194765216512934 1102 14-15 0.38553223073400744 1102 16-17 0.38850491395936615 1102 18-19 0.4102566469799811 1102 20-21 0.3329948757621395 1102 22-23 0.4673408413243152 1102 24-25 0.3411457781588467 1102 26-27 0.33943029940396485 1102 28-29 0.3957919410809865 1102 30-31 0.5940846748489363 1102 32-33 0.5689269884384984 1102 34-35 0.601598097327404 1102 36-37 0.7617633940941673 1102 38-39 0.8462465164058912 1102 40-41 0.8340268588085848 1102 42-43 0.747031594346673 1102 44-45 0.6874539602527463 1102 46-47 0.8594779745368513 1102 48-49 0.9748927672508856 1102 50-51 0.8668286176068349 1102 52-53 0.8869842993877626 1102 54-55 0.9102565424224451 1102 56-57 0.8948221040601254 1102 58-59 0.8881161876869186 1102 60-61 1.0129023315482328 1102 62-63 1.0937889933657203 1102 64-65 1.0785923317727466 1102 66-67 0.9858417039340601 1102 68-69 1.0991506826450674 1102 70-71 1.0218465213274222 1102 72-73 1.0369384376080824 1102 74-75 1.323278308486426 1102 76-77 1.3488885682022307 1102 78-79 1.2485843810127868 1102 80-81 1.4735558615000848 1102 82-83 1.5005421044881153 1102 84-85 1.4393004376161151 1102 86-87 1.3262299032498674 1102 88-89 1.3104826173177173 1102 90-91 1.495260131597533 1102 92-93 1.5668126227873422 1102 94-95 1.63696136232047 1102 96-97 1.6146755953720877 1102 98-99 1.8732032802007161 1102 100-101 1.5931741549604048 1103 1 0.22895369793045361 1103 2 -0.00352286792062273 1103 3 0.1360283976829848 1103 4 0.170973470336925 1103 5 0.13432436989904772 1103 6 0.29797625143686446 1103 7 0.15411174557977603 1103 8 0.17017155954014385 1103 9 0.25789464326505396 1103 10-11 -0.005046963343261268 1103 12-13 0.1894124833352535 1103 14-15 0.3150319533535466 1103 16-17 0.3252732454405489 1103 18-19 0.4285017236982753 1103 20-21 0.3628013120523619 1103 22-23 0.41921798925051945 1103 24-25 0.3973485547993292 1103 26-27 0.27837646632496416 1103 28-29 0.37415715101280256 1103 30-31 0.33008582336358216 1103 32-33 0.2966426140865366 1103 34-35 0.32584469476415734 1103 36-37 0.5196643063480053 1103 38-39 0.33754979693944875 1103 40-41 0.3405656823254901 1103 42-43 0.41126918799580636 1103 44-45 0.39414715973450143 1103 46-47 0.4884470408547159 1103 48-49 0.30805151926920615 1103 50-51 0.10270436888252021 1103 52-53 0.14261343904749424 1103 54-55 0.12322403484986921 1103 56-57 0.03831686750687169 1103 58-59 0.24315343465478634 1103 60-61 0.435462668445858 1103 62-63 0.49194162174502054 1103 64-65 0.15745257278358338 1103 66-67 0.1633532378214433 1103 68-69 0.23941436246470715 1103 70-71 0.4591696137385384 1103 72-73 0.4406501958812008 1103 74-75 0.2273210622984152 1103 76-77 0.4407772721708092 1103 78-79 0.5216410782887877 1103 80-81 0.6320774679317651 1103 82-83 0.4799774003990116 1103 84-85 0.510785725817076 1103 86-87 0.35748306033583077 1103 88-89 0.5636317274847293 1103 90-91 0.6356403466604412 1103 92-93 0.7419961104080208 1103 94-95 0.6841089517690229 1103 96-97 0.9105160404569581 1103 98-99 0.9314939430248224 1103 100-101 0.6812464404868201 1104 1 0.17104573830740932 1104 2 0.10419623510557585 1104 3 0.0582170607579755 1104 4 0.09138415136106914 1104 5 -0.12783899710478863 1104 6 -0.007869490909513388 1104 7 0.08861323673478694 1104 8 0.0996812235378215 1104 9 0.04305946513855474 1104 10-11 0.07723179106713474 1104 12-13 0.11624653488809855 1104 14-15 0.15621357707103556 1104 16-17 0.19566111853167456 1104 18-19 0.13337052624724066 1104 20-21 -4.772246459410212E-4 1104 22-23 0.06009585276922991 1104 24-25 0.23206879476643394 1104 26-27 0.26526536058945993 1104 28-29 0.18799430928257266 1104 30-31 0.4334368616428961 1104 32-33 0.3644867114971433 1104 34-35 0.6098863987241003 1104 36-37 0.6479710246665746 1104 38-39 0.7486757485658941 1104 40-41 0.8462875361266384 1104 42-43 0.9087706826065656 1104 44-45 1.201790754679429 1104 46-47 0.9514731352035284 1104 48-49 1.1889975646846338 1104 50-51 1.0731516808293833 1104 52-53 1.1603561102711382 1104 54-55 0.9586673899659317 1104 56-57 1.1771160989486518 1104 58-59 1.2543258660841161 1104 60-61 1.3735909665248087 1104 62-63 1.2146248022767239 1104 64-65 1.4091813104336026 1104 66-67 1.2236136975049696 1104 68-69 1.1640108175589994 1104 70-71 1.3374607811936912 1104 72-73 1.3818938502649871 1104 74-75 1.5080547520344965 1104 76-77 1.6327103481661531 1104 78-79 1.7651192189584428 1104 80-81 1.8759029112177679 1104 82-83 1.6913144981932113 1104 84-85 1.3998562334318159 1104 86-87 1.5467482993180433 1104 88-89 1.5293075161726897 1104 90-91 1.6694047268894217 1104 92-93 1.8530042210472324 1104 94-95 1.9385210772955936 1104 96-97 2.3170579431223786 1104 98-99 2.745078831732478 1104 100-101 2.0808137809090397 1105 1 0.10266929736951269 1105 2 0.025160122992780032 1105 3 0.07986927016305856 1105 4 0.17643168398402054 1105 5 0.038396395381134596 1105 6 0.0036263106066130035 1105 7 -0.023566923596177958 1105 8 -0.08837677458385684 1105 9 -0.14279923279648443 1105 10-11 -0.02738158781086497 1105 12-13 0.15522566333118704 1105 14-15 0.31726971668498294 1105 16-17 0.1613639237291551 1105 18-19 0.23488110864570189 1105 20-21 0.3006287657722382 1105 22-23 -0.057344795845999386 1105 24-25 0.05324389472561819 1105 26-27 0.1120078205562578 1105 28-29 0.11960994293860949 1105 30-31 0.01352507141253767 1105 32-33 0.03463923593711371 1105 34-35 0.038868816210715806 1105 36-37 -0.16446829644313965 1105 38-39 -0.12788803312086117 1105 40-41 0.2514917540277537 1105 42-43 0.15894801356756005 1105 44-45 0.3829635562708589 1105 46-47 0.38795100548841077 1105 48-49 0.4496522824807414 1105 50-51 0.5278025072511028 1105 52-53 0.7004864039470675 1105 54-55 0.8795163594650077 1105 56-57 0.8844090576216175 1105 58-59 1.1226947778862382 1105 60-61 1.0683261192671978 1105 62-63 0.875676979187638 1105 64-65 0.9055693066135575 1105 66-67 1.1468521769365516 1105 68-69 1.2163759568678287 1105 70-71 0.7256015998088863 1105 72-73 1.2071069446209073 1105 74-75 0.865417256145804 1105 76-77 0.7725462130062759 1105 78-79 0.7290427704914144 1105 80-81 1.112444581021574 1105 82-83 0.9968329584453173 1105 84-85 0.9073794251513512 1105 86-87 0.6360696071685545 1105 88-89 0.6883704161580262 1105 90-91 0.5319603533333321 1105 92-93 0.9808529091943932 1105 94-95 1.2518361557769246 1105 96-97 1.1084909754223702 1105 98-99 1.2839866315208681 1105 100-101 0.7444199199285855 1106 1 -0.16697331481346467 1106 2 -0.16637198865359437 1106 3 -0.2736452988977618 1106 4 -0.09676140549607481 1106 5 0.14978455131164736 1106 6 0.13078181344295814 1106 7 -0.01631265293683981 1106 8 0.052246334181788257 1106 9 0.2923440370317252 1106 10-11 0.02125188189307181 1106 12-13 0.05203184761709423 1106 14-15 -0.10150101124354904 1106 16-17 0.022415187779763812 1106 18-19 0.10899248066309752 1106 20-21 -0.050682893511044824 1106 22-23 0.041096221849905135 1106 24-25 -0.08018949751335924 1106 26-27 -0.09529970726254078 1106 28-29 0.1493371210353871 1106 30-31 0.21289244091232007 1106 32-33 0.3409243876796708 1106 34-35 0.3064860424810192 1106 36-37 0.34504940349311397 1106 38-39 0.1717117312565506 1106 40-41 0.4640483397596853 1106 42-43 0.4555984610083996 1106 44-45 0.5657203405067364 1106 46-47 0.4061270284696761 1106 48-49 0.4546875048293799 1106 50-51 0.18345865966340824 1106 52-53 0.5428734543880509 1106 54-55 0.4776910913636243 1106 56-57 0.27891550093098516 1106 58-59 0.42591360832673786 1106 60-61 0.7416580001325457 1106 62-63 0.4861291001943542 1106 64-65 0.47701459295148396 1106 66-67 0.5972463606092013 1106 68-69 1.061764774478192 1106 70-71 0.8443529614976235 1106 72-73 0.8863495632593015 1106 74-75 1.194009895360825 1106 76-77 0.5764549657397389 1106 78-79 1.0081829094441837 1106 80-81 1.4962713500065412 1106 82-83 1.3245258744361834 1106 84-85 1.0738403255201163 1106 86-87 1.0564299806142117 1106 88-89 0.849880821477285 1106 90-91 0.8470127632197979 1106 92-93 1.6739787009911744 1106 94-95 1.4177322074847858 1106 96-97 1.6479093426869689 1106 98-99 1.040137429974795 1106 100-101 0.6618350110709521 1107 1 0.03799212946615427 1107 2 -0.02099647549252026 1107 3 0.036413233087841945 1107 4 0.016519260549621606 1107 5 0.1376526761032153 1107 6 0.07464170465856057 1107 7 -0.132824652568857 1107 8 0.028718545862886913 1107 9 -0.21131852434636755 1107 10-11 -0.32421332664868885 1107 12-13 0.07644509033509195 1107 14-15 -0.17915651198526206 1107 16-17 -0.30646787474251624 1107 18-19 -0.0781028839256308 1107 20-21 -0.10409764265146748 1107 22-23 -0.1085915692288566 1107 24-25 0.02699648240866992 1107 26-27 0.32076036564370725 1107 28-29 0.12950064261403327 1107 30-31 0.04494359027813033 1107 32-33 0.2325597184601378 1107 34-35 0.1279404875770993 1107 36-37 0.041298296675115864 1107 38-39 0.43262754409219184 1107 40-41 0.3894631808953619 1107 42-43 0.3053874468756206 1107 44-45 0.10332340394896988 1107 46-47 0.16265006753317124 1107 48-49 0.41806764075238334 1107 50-51 0.5466042076676914 1107 52-53 0.2567509396975538 1107 54-55 0.24908861439118368 1107 56-57 0.37123849629671923 1107 58-59 0.30821141998943347 1107 60-61 0.05637379226924821 1107 62-63 -0.03816914844583863 1107 64-65 0.23395161069506898 1107 66-67 0.4244562592994221 1107 68-69 0.593163907422845 1107 70-71 0.38031735933113353 1107 72-73 0.3697156699048101 1107 74-75 0.2525303815557862 1107 76-77 0.5256620815732767 1107 78-79 0.7124986371842041 1107 80-81 0.8560360721233486 1107 82-83 0.8752267633404038 1107 84-85 0.523656909966018 1107 86-87 0.6339014908314375 1107 88-89 0.909177517697433 1107 90-91 0.8224155299773095 1107 92-93 1.2722238913638684 1107 94-95 0.7139857004298982 1107 96-97 0.510014194287109 1107 98-99 0.7090694799742181 1107 100-101 0.5475941444755854 1108 1 -0.20228536619797666 1108 2 -0.37886588378301767 1108 3 -0.17080227916978075 1108 4 0.02233166973073253 1108 5 0.03135746196593914 1108 6 -0.06455924532659196 1108 7 -0.3310871797168673 1108 8 -0.013952662399482563 1108 9 -0.352300696499924 1108 10-11 -0.349791051996732 1108 12-13 -0.3715869716639375 1108 14-15 -0.43977743978326345 1108 16-17 -0.31591303966180817 1108 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-1.3058968766235353 2316 48-49 -1.4272182533140025 2316 50-51 -1.0246445830932984 2316 52-53 -1.4312996385969257 2316 54-55 -1.2466137715551042 2316 56-57 -1.1915477616114885 2316 58-59 -1.0573212016407467 2316 60-61 -0.9305835483466254 2316 62-63 -0.6948822441833258 2316 64-65 -0.5501746124555424 2316 66-67 -0.7962673150826909 2316 68-69 -0.666306681316037 2316 70-71 -0.3628745180070858 2316 72-73 -0.2910938567583763 2316 74-75 -0.7154986230585862 2316 76-77 -0.852896077472689 2316 78-79 -0.44980478121677336 2316 80-81 -0.6484242006296057 2316 82-83 -0.8980355708975409 2316 84-85 -0.9516703336539116 2316 86-87 -0.611931450123457 2316 88-89 -0.7075343795022739 2316 90-91 -0.8903677315864407 2316 92-93 -0.5865284308030887 2316 94-95 -1.0181667878201495 2316 96-97 -1.2550552780994337 2316 98-99 -1.1598447304034032 2316 100-101 -0.9883705500256958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 16.0 6 44.0 7 80.0 8 233.0 9 373.0 10 672.0 11 1175.0 12 1586.0 13 2307.0 14 2887.0 15 4123.0 16 5439.0 17 7654.0 18 6868.0 19 3913.0 20 3346.0 21 3253.0 22 3400.0 23 3593.0 24 4147.0 25 4700.0 26 5720.0 27 6761.0 28 8282.0 29 9973.0 30 12374.0 31 15207.0 32 18703.0 33 24323.0 34 31975.0 35 44496.0 36 64919.0 37 95625.0 38 71162.0 39 5407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52631822912936 20.185455970536058 15.608473412078025 20.67975238825656 2 15.374406464741794 22.260468761191326 39.52786824465239 22.83725652941449 3 18.79060459237413 27.366968611754793 31.04613440067411 22.796292395196964 4 12.561639132238977 18.408430737731678 38.266921403128876 30.763008726900466 5 12.743856840420042 38.85868363123137 33.60711163540229 14.790347892946293 6 29.14949056218259 38.17044734053869 17.632004111732975 15.048057985545743 7 26.27970316321179 35.35395238225635 21.36858357960901 16.99776087492285 8 26.406930966278313 33.786986112369114 20.05354521115813 19.752537710194442 9 26.922161440286136 16.846941161353925 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34-35 21.252221025036626 26.52706991107474 32.00761704946764 20.213092014420997 36-37 21.324478198355727 26.111096481295192 32.20997414033739 20.354451180011694 38-39 20.873963316628434 26.816420938212932 32.052659407513204 20.25695633764543 40-41 20.82281814160224 26.84259863916918 32.02197130879906 20.312611910429528 42-43 20.73589621781955 26.97706802278978 31.314773013346702 20.972262746043967 44-45 21.105121691249266 27.014635810973065 30.89013451970189 20.990107978075777 46-47 21.513725184552467 27.33156460679187 30.304836754404352 20.849873454251316 48-49 21.710587984997836 27.57421826864238 29.708582640924398 21.00661110543539 50-51 21.177676266471742 27.78227755840616 29.82276715794441 21.21727901717769 52-53 21.564635451335935 28.35468609128673 29.032974949654957 21.04770350772238 54-55 21.175216623361305 27.893358217679754 29.32922853962098 21.60219661933796 56-57 21.567972801611006 28.08741316189698 29.031942098101254 21.31267193839076 58-59 21.85554827661022 27.551802802838647 29.204837245356863 21.387811675194268 60-61 21.61345497210046 27.537130928784027 29.804166078624256 21.045248020491258 62-63 21.156068024817873 27.662808051169225 29.910677141205106 21.2704467828078 64-65 21.01210677216643 27.890025764020116 29.580065431763263 21.517802032050188 66-67 21.184389573950888 27.802306133540043 29.125678801181294 21.887625491327775 68-69 21.10381813815737 27.635897155038403 29.334111312861516 21.92617339394271 70-71 21.31982208291071 27.75725671481129 29.147668445626906 21.77525275665109 72-73 21.287691896071173 27.42352071224069 29.454717056656083 21.834070335032056 74-75 21.037325867738478 28.2529791810175 28.825854045935316 21.883840905308706 76-77 21.071695648968326 27.95445688465711 28.785797794466156 22.18804967190841 78-79 21.142065036950896 27.86095592014898 28.635681843078764 22.361297199821355 80-81 21.396034650728033 28.12617499144264 28.174412175149428 22.303378182679904 82-83 21.028677719812137 27.79275194251606 28.666555018732627 22.512015318939177 84-85 20.652760048700546 27.95952714971205 28.292763587494573 23.094949214092825 86-87 20.859777688371928 27.840560813415372 28.19433414992238 23.105327348290324 88-89 20.8774148973481 27.534279614648575 28.028554847910048 23.559750640093274 90-91 20.800363150350353 27.55771910404658 28.343316106160394 23.29860163944268 92-93 20.707189326334394 27.80183195590836 28.17603796616453 23.31494075159272 94-95 20.45545745331835 27.65110383448328 28.14917484936222 23.744263862836153 96-97 20.471229333294012 27.941997358537463 28.039630363759304 23.54714294440922 98-99 20.480729836446113 28.259044754300543 27.176654839052823 24.08357057020052 100-101 20.09032483659167 27.828917850319446 27.395830480840726 24.68492683224816 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 616.0 1 434.0 2 266.5 3 294.0 4 272.5 5 256.0 6 405.0 7 805.5 8 1296.0 9 1539.5 10 1376.0 11 1170.5 12 1125.0 13 1169.0 14 1241.5 15 1281.5 16 1353.5 17 1410.5 18 1499.5 19 1597.5 20 1630.5 21 1794.5 22 2050.0 23 2233.5 24 2478.5 25 2909.5 26 3600.0 27 4230.5 28 4651.5 29 5181.5 30 5880.5 31 6703.0 32 7699.0 33 8808.0 34 10193.5 35 11215.0 36 12289.5 37 13363.0 38 13800.5 39 13828.5 40 13841.5 41 14440.5 42 15183.5 43 15855.5 44 16651.5 45 17243.0 46 16969.0 47 17889.0 48 20808.0 49 20264.5 50 17276.0 51 15636.5 52 14385.5 53 13708.0 54 13098.5 55 12107.0 56 11025.5 57 9914.5 58 8846.0 59 7873.5 60 6802.0 61 5551.0 62 4418.5 63 3377.5 64 2344.5 65 1707.0 66 1222.0 67 939.5 68 671.5 69 359.0 70 221.5 71 145.5 72 97.5 73 78.0 74 53.0 75 35.5 76 27.0 77 12.5 78 5.0 79 3.5 80 1.5 81 0.0 82 1.5 83 1.5 84 0.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02633025725714551 2 0.008636324380343726 3 0.008215040264229399 4 0.0 5 0.005055409393371937 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.1596308708574605E-4 14-15 0.0 16-17 1.0532102902858202E-4 18-19 0.0040021991030861175 20-21 0.0011585313193144021 22-23 0.0 24-25 0.005266051451429101 26-27 0.005582014538514847 28-29 0.0058979776256005935 30-31 0.025277046966859687 32-33 3.1596308708574605E-4 34-35 0.0030543098418288786 36-37 0.012954486570515587 38-39 0.0058979776256005935 40-41 0.006108619683657757 42-43 0.011058708048001112 44-45 0.0027383467547431326 46-47 0.0020010995515430587 48-49 0.0015798154354287304 50-51 0.005160730422400519 52-53 0.003159630870857461 54-55 0.0020010995515430587 56-57 0.0010532102902858203 58-59 0.0012638523483429842 60-61 0.0 62-63 1.0532102902858202E-4 64-65 0.009689534670629545 66-67 0.0010532102902858203 68-69 0.0010532102902858203 70-71 0.004950088364343355 72-73 0.014323659947887155 74-75 0.0058979776256005935 76-77 0.005055409393371937 78-79 0.011585313193144021 80-81 3.1596308708574605E-4 82-83 0.006845866886857831 84-85 2.1064205805716404E-4 86-87 0.0 88-89 7.372472032000742E-4 90-91 9.478892612572382E-4 92-93 1.0532102902858202E-4 94-95 0.0015798154354287304 96-97 4.212841161143281E-4 98-99 5.266051451429101E-4 100-101 0.0016851364644573123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 474739.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.69247014322636 #Duplication Level Percentage of deduplicated Percentage of total 1 82.06197393096932 49.80543902699574 2 10.470459025458505 12.709560435770307 3 3.038227087496725 5.531925203887098 4 1.3180637422250083 3.199861772874421 5 0.74651628183941 2.2653958573485378 6 0.46692095897967256 1.700315181727224 7 0.32092394964395754 1.3634367062408523 8 0.24204146579658178 1.175207554902543 9 0.17722086273335758 0.9680374728181009 >10 1.0590816878418328 11.748184096047767 >50 0.0652512300898012 2.627041576713678 >100 0.02950188581977361 3.1764220832824472 >500 0.0024295670675107683 1.078117131258012 >1k 0.0013883240385775817 2.651055900133271 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4716 0.9933879457975856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3642 0.7671583754441914 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2452 0.5164943263561662 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1775 0.3738896530514662 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 936 0.19716096634150557 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 866 0.18241602227750406 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.17567547641967482 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 736 0.1550325547300727 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 622 0.13101936011155604 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 609 0.1282810133568129 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 515 0.10848065989943949 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.1596308708574605E-4 0.0 12-13 0.0 0.0 0.0 4.212841161143281E-4 0.0 14-15 0.0 0.0 0.0 6.319261741714921E-4 0.0 16-17 0.0 0.0 0.0 6.319261741714921E-4 0.0 18-19 0.0 0.0 0.0 8.425682322286561E-4 0.0 20-21 0.0 0.0 0.0 0.0011585313193144021 0.0 22-23 0.0 0.0 0.0 0.0017904574934858946 0.0 24-25 0.0 0.0 0.0 0.003686236016000371 0.0 26-27 0.0 0.0 0.0 0.005897977625600593 0.0 28-29 0.0 0.0 0.0 0.010110818786743875 0.0 30-31 0.0 0.0 0.0 0.016535401557487377 0.0 32-33 0.0 0.0 0.0 0.02432915770560245 0.0 34-35 0.0 0.0 0.0 0.029911172244117296 0.0 36-37 0.0 0.0 0.0 0.035493186782632136 0.0 38-39 0.0 0.0 0.0 0.048131710266061986 0.0 40-41 0.0 0.0 0.0 0.0743566464941789 0.0 42-43 0.0 0.0 0.0 0.12017129412161208 0.0 44-45 0.0 0.0 0.0 0.1635635580813879 0.0 46-47 0.0 0.0 0.0 0.2666728455003697 0.0 48-49 0.0 0.0 0.0 0.36346287117763654 0.0 50-51 0.0 0.0 0.0 0.43097365078495764 0.0 52-53 0.0 0.0 0.0 0.5202858834011952 0.0 54-55 0.0 0.0 0.0 0.6005405075209747 0.0 56-57 0.0 0.0 0.0 0.7951737691657943 0.0 58-59 0.0 0.0 0.0 1.0581603786501637 0.0 60-61 0.0 0.0 0.0 1.3373664266049343 0.0 62-63 0.0 0.0 0.0 1.6019328515247326 0.0 64-65 0.0 0.0 0.0 1.928638683571394 0.0 66-67 0.0 0.0 0.0 2.3273840994736057 0.0 68-69 0.0 0.0 0.0 2.7718388419742217 0.0 70-71 0.0 0.0 0.0 3.1417262959226018 0.0 72-73 0.0 0.0 0.0 3.463903323721034 0.0 74-75 0.0 0.0 0.0 3.8070392362961547 0.0 76-77 0.0 0.0 0.0 4.2071538255757375 0.0 78-79 0.0 0.0 0.0 4.612850429393836 0.0 80-81 0.0 0.0 0.0 5.104278350841199 0.0 82-83 0.0 0.0 0.0 5.548627772312786 0.0 84-85 0.0 1.0532102902858202E-4 0.0 6.200354299941652 0.0 86-87 0.0 2.1064205805716404E-4 0.0 6.888837866701493 0.0 88-89 0.0 2.1064205805716404E-4 0.0 7.801865867350271 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCGTA 30 0.0039508236 31.666315 80-81 CGGTATC 65 0.0058289594 29.242765 2 TGCACCG 85 6.4020103E-4 27.940866 5 GTATCAA 5580 0.0 27.339363 1 GTACATG 2710 0.0 26.12971 1 GATATAC 110 9.4320014E-5 25.922459 1 CCTATAC 110 9.4455296E-5 25.916994 3 TACATGG 2830 0.0 24.851185 2 ACATGGG 2740 0.0 24.104065 3 GAGTACT 3450 0.0 24.092665 12-13 GGTATCA 4015 0.0 23.91021 1 AGTACTT 3495 0.0 23.374762 12-13 CAACGCA 6645 0.0 22.94557 5 TCAACGC 6670 0.0 22.859566 4 CATGGGA 1790 0.0 22.820976 4 ATCAACG 6695 0.0 22.781404 3 GTACTTT 3650 0.0 22.708652 14-15 AACGCAG 6735 0.0 22.70708 6 TATCAAC 6760 0.0 22.635027 2 GTCTAGA 105 0.0021877675 22.630716 1 >>END_MODULE