FastQCFastQC Report
Thu 26 May 2016
SRR937121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517894
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43770.8451536414787582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30930.5972264594685399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.41379123913387683No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13750.26549834522122284No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11020.21278485558820914No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8610.16625023653488938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7370.14230711303857546No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6310.12183960424333937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.11971561748156957No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.1175916307197998No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGT550.002573157734.5595786
TACGCGA600.00394479931.6734948
TTACGCT650.005823983329.2483734
GTATCAA54100.028.5495431
CTTTGCG700.00836278927.15922
TTTGCGT908.9095044E-426.4047783
GGTATCA37800.026.0250551
GAGTACT29200.025.3797312-13
TATCAAC63600.024.2126852
ATCAACG63750.024.0066053
TCAACGC63800.023.9877914
CAACGCA64300.023.8751775
AACGCAG65000.023.6134976
GTACTTT31700.023.22831514-15
GTACATG31050.023.1115171
AGTACTT31150.023.10467512-13
GGCGTGC1252.250291E-422.8049148
AGACCCG1050.002186936322.632675
ACGCAGA70650.021.5863957
AGAGTAC49450.021.47224610-11
Produced by FastQC (version 0.11.5)