##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937121_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 517894 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.708042572418293 33.0 31.0 34.0 30.0 34.0 2 31.808617207382206 33.0 31.0 34.0 30.0 34.0 3 31.78001676018645 33.0 31.0 34.0 30.0 34.0 4 35.387633376714156 37.0 35.0 37.0 33.0 37.0 5 35.3857816464373 37.0 35.0 37.0 33.0 37.0 6 35.4881230522076 37.0 35.0 37.0 33.0 37.0 7 35.4148513016177 37.0 35.0 37.0 33.0 37.0 8 35.36769686460936 37.0 35.0 37.0 33.0 37.0 9 36.97104812954002 39.0 37.0 39.0 33.0 39.0 10-11 36.84962752995787 39.0 37.0 39.0 33.0 39.0 12-13 36.76265509930604 39.0 37.0 39.0 32.5 39.0 14-15 37.85860813216604 40.0 37.5 41.0 32.5 41.0 16-17 37.818406855456914 40.0 37.5 41.0 33.0 41.0 18-19 37.81556264409319 40.0 37.5 41.0 32.5 41.0 20-21 37.75338679343649 40.0 37.5 41.0 32.0 41.0 22-23 37.60966143651018 40.0 37.0 41.0 32.0 41.0 24-25 37.680169107964176 40.0 37.0 41.0 32.0 41.0 26-27 37.617741275241656 40.0 37.0 41.0 32.0 41.0 28-29 37.512276643483034 40.0 37.0 41.0 32.0 41.0 30-31 37.27787249900559 39.5 36.5 41.0 32.0 41.0 32-33 37.01499727743516 39.0 36.0 41.0 31.0 41.0 34-35 36.76871715061384 39.0 35.0 41.0 30.5 41.0 36-37 36.55300022784586 39.0 35.0 41.0 30.0 41.0 38-39 36.38049581574608 39.0 35.0 41.0 30.0 41.0 40-41 36.18125523755826 39.0 35.0 40.5 30.0 41.0 42-43 35.88094571476016 39.0 35.0 40.0 29.5 41.0 44-45 35.540139488003334 38.5 35.0 40.0 27.5 41.0 46-47 35.0662471470996 38.0 35.0 40.0 25.5 41.0 48-49 34.71425909549058 38.5 34.0 40.0 24.0 41.0 50-51 33.96040019772386 38.0 33.5 39.5 22.5 40.5 52-53 34.126215982421115 38.0 33.5 39.5 23.0 40.5 54-55 34.51415347542161 38.5 34.0 40.0 23.0 41.0 56-57 34.40796861906104 38.0 34.0 40.0 22.5 41.0 58-59 34.1520803484883 38.0 34.0 40.0 21.5 41.0 60-61 33.88580385175345 38.0 34.0 40.0 20.5 41.0 62-63 33.55020332346001 37.5 33.5 40.0 18.5 41.0 64-65 33.20011527455425 37.0 33.0 40.0 17.5 41.0 66-67 32.812819804824926 36.5 32.5 39.5 13.5 41.0 68-69 32.39908745805126 36.0 32.0 39.0 9.5 41.0 70-71 32.00010619933809 35.5 32.0 39.0 7.5 40.5 72-73 31.593552927819204 35.0 31.0 38.0 7.0 40.0 74-75 31.171214572866262 35.0 31.0 37.0 2.0 39.5 76-77 30.741922092165577 35.0 31.0 37.0 2.0 39.0 78-79 30.312057100487745 35.0 30.5 36.0 2.0 39.0 80-81 29.857965143446343 34.5 30.0 36.0 2.0 37.5 82-83 29.43959188559821 34.0 29.5 35.0 2.0 37.0 84-85 29.057171930935674 34.0 29.0 35.0 2.0 37.0 86-87 28.720775873055103 34.0 29.0 35.0 2.0 36.0 88-89 28.3458255936543 34.0 29.0 35.0 2.0 36.0 90-91 27.920273260551387 34.0 27.5 35.0 2.0 35.5 92-93 27.570632214314127 34.0 27.0 35.0 2.0 35.0 94-95 27.177327792946045 34.0 26.0 35.0 2.0 35.0 96-97 26.66602914882196 33.5 25.0 35.0 2.0 35.0 98-99 26.026834641837905 33.0 24.5 35.0 2.0 35.0 100-101 23.78868262617447 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5194012650335473 1101 2 0.5523382476192253 1101 3 0.355903552558523 1101 4 0.4889849755293483 1101 5 0.4277639608543282 1101 6 0.3999528796978282 1101 7 0.37108077636437287 1101 8 0.3767585919620089 1101 9 0.5644906857465841 1101 10-11 0.5753927284606632 1101 12-13 0.6534128423328056 1101 14-15 0.728936369159932 1101 16-17 0.6967060620269478 1101 18-19 0.7430975691758164 1101 20-21 0.8091690701776884 1101 22-23 0.8710031682133419 1101 24-25 0.8661535732989591 1101 26-27 0.816155366586834 1101 28-29 0.5929937318496599 1101 30-31 0.690165917013033 1101 32-33 0.6520371596212087 1101 34-35 0.7134299893318712 1101 36-37 0.7530347246084332 1101 38-39 0.7959618906022286 1101 40-41 0.9688393251833389 1101 42-43 0.9776214423468517 1101 44-45 1.034857601325207 1101 46-47 1.0875428328072445 1101 48-49 1.206926705688204 1101 50-51 1.1575452028411277 1101 52-53 1.28262313033958 1101 54-55 1.3035217529752856 1101 56-57 1.1905712702638098 1101 58-59 1.2842373718649966 1101 60-61 1.458803754890802 1101 62-63 1.4191873906103254 1101 64-65 1.3697763892597195 1101 66-67 1.3035169168696896 1101 68-69 1.3692009034724606 1101 70-71 1.2052176753431816 1101 72-73 1.2114780710994815 1101 74-75 1.2482576195632191 1101 76-77 1.41189633287674 1101 78-79 1.4141491087971012 1101 80-81 1.2413746847492533 1101 82-83 1.3870031246738037 1101 84-85 1.3593840231350498 1101 86-87 1.3370946144465634 1101 88-89 1.34299123233858 1101 90-91 1.474899657646926 1101 92-93 1.6381932554747678 1101 94-95 1.7080236813557619 1101 96-97 1.5209455002040428 1101 98-99 1.368645131301907 1101 100-101 1.6478524694284573 1102 1 0.4914819241925663 1102 2 0.40167966520142784 1102 3 0.38730763637568444 1102 4 0.431658957642874 1102 5 0.5419251461104793 1102 6 0.39333674697893883 1102 7 0.4428380951377733 1102 8 0.4244494507582175 1102 9 0.5886813368183965 1102 10-11 0.5904531163804165 1102 12-13 0.6987174579539683 1102 14-15 0.802677049283588 1102 16-17 0.8313957395292917 1102 18-19 0.7117991956356207 1102 20-21 0.6584646553321178 1102 22-23 0.6762752280261424 1102 24-25 0.3565309811646955 1102 26-27 0.5887666469822435 1102 28-29 0.5832735609981938 1102 30-31 0.5986725634542935 1102 32-33 0.9184004451853269 1102 34-35 0.7287631541201449 1102 36-37 0.7235415573363255 1102 38-39 0.7178553021168099 1102 40-41 0.9311946388619532 1102 42-43 0.9016771095265455 1102 44-45 0.7905648474320444 1102 46-47 1.07105202960134 1102 48-49 1.160341739020204 1102 50-51 1.2882208467497733 1102 52-53 1.4958451943660052 1102 54-55 1.5384048137535231 1102 56-57 1.53601914918341 1102 58-59 1.6285091315493077 1102 60-61 1.8077607714658868 1102 62-63 1.9666860489029574 1102 64-65 1.7362421324768889 1102 66-67 1.7589057015267713 1102 68-69 2.0125196443521816 1102 70-71 1.8299277113142445 1102 72-73 1.7029037109423477 1102 74-75 1.7428449576897371 1102 76-77 1.977807799410936 1102 78-79 1.6985518900862324 1102 80-81 1.8294180392689974 1102 82-83 2.010070585572091 1102 84-85 2.1198896287366615 1102 86-87 1.9617898657635067 1102 88-89 1.885430581991212 1102 90-91 1.7962943827780329 1102 92-93 1.6372669968941231 1102 94-95 1.793161112108141 1102 96-97 1.8883360235364428 1102 98-99 1.785481451874606 1102 100-101 1.89402213516221 1103 1 0.28397141109446267 1103 2 0.45663941715859835 1103 3 0.4338944697789806 1103 4 0.4918424857723167 1103 5 0.3569299699838666 1103 6 0.38149800427919445 1103 7 0.3479874879189069 1103 8 0.5160220334821233 1103 9 0.33929444744343584 1103 10-11 0.5477817215813587 1103 12-13 0.6095273531543981 1103 14-15 0.8141748067348189 1103 16-17 0.7639513613670772 1103 18-19 0.6861954749931272 1103 20-21 0.6309645264500077 1103 22-23 0.5618625254050471 1103 24-25 0.47727179555235466 1103 26-27 0.37368928089075126 1103 28-29 0.5583812696215134 1103 30-31 0.6220900752193899 1103 32-33 0.7788962362637406 1103 34-35 0.6387065932841125 1103 36-37 0.8433963992532156 1103 38-39 1.0385007437405775 1103 40-41 1.0488452284055612 1103 42-43 1.17437730027207 1103 44-45 1.1491539197017673 1103 46-47 1.4990883080750805 1103 48-49 1.390513409995215 1103 50-51 1.418759279395978 1103 52-53 1.5488669275726679 1103 54-55 1.3820936407790043 1103 56-57 1.5364945400644459 1103 58-59 1.510568373133303 1103 60-61 1.7141724724888334 1103 62-63 1.6997166933871668 1103 64-65 1.572160472769859 1103 66-67 1.4362681106648765 1103 68-69 1.6462683463251793 1103 70-71 1.417749456304417 1103 72-73 1.2913775994261805 1103 74-75 1.5078851320856472 1103 76-77 1.5800595540488516 1103 78-79 1.5353028088315845 1103 80-81 1.431496723640386 1103 82-83 1.5728295465613193 1103 84-85 1.823849255494057 1103 86-87 1.8428438268632021 1103 88-89 1.7742701274424135 1103 90-91 1.5433805423119935 1103 92-93 1.647291815906815 1103 94-95 1.6181705020906847 1103 96-97 1.7361851651815705 1103 98-99 1.8051705882171518 1103 100-101 1.719003539314425 1104 1 0.1919844926213301 1104 2 0.3062897577694983 1104 3 0.2706869104144438 1104 4 0.2516400581925282 1104 5 0.14218869207897455 1104 6 0.33706347008237714 1104 7 0.3385085515039137 1104 8 0.30538618337676837 1104 9 0.3421317944270612 1104 10-11 0.37496014481786943 1104 12-13 0.5119006420208834 1104 14-15 0.5374798312303639 1104 16-17 0.4227212841964132 1104 18-19 0.2975480945301001 1104 20-21 0.4705393768268138 1104 22-23 0.37781759320406394 1104 24-25 0.47980084765870856 1104 26-27 0.4552672077531241 1104 28-29 0.5850245394861773 1104 30-31 0.35574269087844357 1104 32-33 0.5470752928943412 1104 34-35 0.5086352395011957 1104 36-37 0.6189389853107841 1104 38-39 0.5986874011002286 1104 40-41 0.5535097234353543 1104 42-43 0.5678009892109372 1104 44-45 0.48706190951575223 1104 46-47 0.5398924600379544 1104 48-49 0.7610450732363816 1104 50-51 0.605270570615069 1104 52-53 0.4660131640484124 1104 54-55 0.6537977294024699 1104 56-57 0.8618025117814625 1104 58-59 1.0827940423981843 1104 60-61 0.890267662571695 1104 62-63 0.9351880549259306 1104 64-65 1.2229405816102314 1104 66-67 1.1802980482303198 1104 68-69 1.347466624527371 1104 70-71 1.1228217795680742 1104 72-73 1.1632671820324063 1104 74-75 1.0877942594237133 1104 76-77 1.0596313020872685 1104 78-79 1.2060626232083393 1104 80-81 1.3163437395786488 1104 82-83 1.4081574249496533 1104 84-85 1.3059429461133654 1104 86-87 1.154227469784054 1104 88-89 1.3594732659747493 1104 90-91 1.4698848532697113 1104 92-93 1.2660415735366293 1104 94-95 1.307325890464675 1104 96-97 1.5629777350811302 1104 98-99 1.4094330718975563 1104 100-101 1.2061786016843534 1105 1 0.26047821057960263 1105 2 0.3431206602798298 1105 3 0.2968172224045702 1105 4 0.23789990364684854 1105 5 0.461707331149384 1105 6 0.37794436389640396 1105 7 0.1881370952463044 1105 8 0.1816254061759821 1105 9 0.25713167019419814 1105 10-11 0.3967837175893436 1105 12-13 0.5043638483882305 1105 14-15 0.31015854020245825 1105 16-17 0.6009913001810361 1105 18-19 0.3443921657238178 1105 20-21 0.10876500026872549 1105 22-23 0.1918492808660872 1105 24-25 -0.06765610565457791 1105 26-27 0.0011381215272407985 1105 28-29 -0.05880398603540016 1105 30-31 -0.1343932198726705 1105 32-33 0.2490158101999782 1105 34-35 -0.1885016071374821 1105 36-37 0.09668887971285045 1105 38-39 0.4580392368478243 1105 40-41 0.3537018702620358 1105 42-43 0.6874082477229635 1105 44-45 0.4259890345188566 1105 46-47 -0.27538983843563614 1105 48-49 0.03393497807401502 1105 50-51 -0.10291885965302328 1105 52-53 0.031032502963931563 1105 54-55 -0.039276702646304784 1105 56-57 0.2472515307559675 1105 58-59 0.3180496722173771 1105 60-61 0.22602353671552322 1105 62-63 0.30209324868778964 1105 64-65 0.37827721125550084 1105 66-67 0.08691634690269012 1105 68-69 0.23873388254246564 1105 70-71 0.3523792621133559 1105 72-73 0.06688732763332084 1105 74-75 0.2028587362791292 1105 76-77 0.38632645949030575 1105 78-79 0.6209175606363857 1105 80-81 0.6386907468090008 1105 82-83 0.43090032090049135 1105 84-85 0.5047817829624854 1105 86-87 0.4531027482727197 1105 88-89 0.6362433772045719 1105 90-91 0.5220792197235262 1105 92-93 0.5026968936018896 1105 94-95 0.5512902439154495 1105 96-97 0.43587923498588665 1105 98-99 0.7272787084253345 1105 100-101 0.80676166791703 1106 1 0.39905819347096383 1106 2 0.27300691950740585 1106 3 0.4367278772852856 1106 4 0.3737393358934682 1106 5 0.22305764639191494 1106 6 -0.003657511717442219 1106 7 0.3113509849272589 1106 8 0.13502020388216351 1106 9 0.386666251824586 1106 10-11 0.3938134676131071 1106 12-13 0.39817147123777374 1106 14-15 0.48789037811393854 1106 16-17 0.22847442916117444 1106 18-19 0.2750942536115275 1106 20-21 0.6110144695840631 1106 22-23 0.7142410053536139 1106 24-25 0.3740081848121122 1106 26-27 0.17803828944536804 1106 28-29 0.6999008265616311 1106 30-31 0.7166507239856443 1106 32-33 0.5167343413915617 1106 34-35 0.5628845095180992 1106 36-37 0.291846184151602 1106 38-39 0.29812097812716587 1106 40-41 0.23351367522354138 1106 42-43 0.2890462415765285 1106 44-45 0.24801165594267616 1106 46-47 0.6647140807101977 1106 48-49 0.6854177268621484 1106 50-51 0.7174958664503208 1106 52-53 0.8169923293429306 1106 54-55 0.6125070314727665 1106 56-57 0.827559169446836 1106 58-59 0.4144204227797488 1106 60-61 0.586073278501793 1106 62-63 0.5348499574924048 1106 64-65 0.43212190282474694 1106 66-67 0.22980517242624643 1106 68-69 0.4820963639289033 1106 70-71 0.5455643205678768 1106 72-73 0.433146872036577 1106 74-75 0.7693844150802569 1106 76-77 0.6541558800143363 1106 78-79 0.7043776246353737 1106 80-81 0.23724760135387868 1106 82-83 0.5214969055090535 1106 84-85 0.6005268008631965 1106 86-87 0.7023898882784216 1106 88-89 0.5143284768465577 1106 90-91 0.6043828663984279 1106 92-93 0.3987777477665766 1106 94-95 0.7417426723918297 1106 96-97 0.826391860528453 1106 98-99 0.891188983114052 1106 100-101 0.5623774205387733 1107 1 -0.1453911652198805 1107 2 0.22070295698672737 1107 3 0.016461525130388566 1107 4 0.30109858368614795 1107 5 0.18452176420277766 1107 6 0.16219011388250237 1107 7 -0.05895386215777165 1107 8 0.3846311593754663 1107 9 0.16686020074918417 1107 10-11 0.27084673706124107 1107 12-13 0.1862323096082079 1107 14-15 0.2533866350962626 1107 16-17 0.07852848587157268 1107 18-19 -0.24433749569638508 1107 20-21 0.042059402176981564 1107 22-23 0.18516914269042672 1107 24-25 -0.11108936297893734 1107 26-27 -0.5163037748657402 1107 28-29 0.040171437730080584 1107 30-31 0.11274717426166347 1107 32-33 -0.04241680444688711 1107 34-35 0.09837355893904487 1107 36-37 0.09983429167530744 1107 38-39 0.16452718973462055 1107 40-41 -0.024189406008957803 1107 42-43 -0.433659214875064 1107 44-45 0.16046435632107858 1107 46-47 0.29244353506503984 1107 48-49 -0.2491613679336595 1107 50-51 -0.04121873049956548 1107 52-53 -0.19259454634302386 1107 54-55 0.19792072911556602 1107 56-57 0.3841016694877837 1107 58-59 0.36867288399815124 1107 60-61 0.13938875673986928 1107 62-63 0.21825782356280143 1107 64-65 0.09859199963539567 1107 66-67 0.2736975831917121 1107 68-69 0.44630017410793954 1107 70-71 0.1418344075500393 1107 72-73 0.060995087346089605 1107 74-75 0.25137779722843945 1107 76-77 0.20296253718012736 1107 78-79 0.009425374729563174 1107 80-81 0.3037414503554672 1107 82-83 0.3685327958885942 1107 84-85 -0.003622461180807335 1107 86-87 -0.2048414252996409 1107 88-89 -0.21353693832535825 1107 90-91 0.0793980186581642 1107 92-93 -0.29558888777652825 1107 94-95 -0.318542845324032 1107 96-97 -0.42772791603714566 1107 98-99 -0.38956191373591764 1107 100-101 0.08491743069488322 1108 1 -0.40134707501941236 1108 2 -0.1286591842863558 1108 3 0.12095617853486473 1108 4 0.11684414282806443 1108 5 0.36619622570070476 1108 6 0.004053847057747362 1108 7 -0.1982178544288118 1108 8 -0.1752353404159379 1108 9 -0.30138958516742065 1108 10-11 -0.0791686330285657 1108 12-13 0.2136898771817144 1108 14-15 0.29972196654127004 1108 16-17 0.20787328090045065 1108 18-19 0.3418228362975597 1108 20-21 0.16516284288137228 1108 22-23 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-0.25246226141043593 2316 78-79 -0.24790832350693037 2316 80-81 -0.8063666522051243 2316 82-83 -0.252034913602877 2316 84-85 -0.011037407386098153 2316 86-87 -0.08470561290547707 2316 88-89 -0.35436104930153434 2316 90-91 -0.4707362543007996 2316 92-93 -1.3505937626546576 2316 94-95 -0.9283002987561133 2316 96-97 -1.582462534513791 2316 98-99 -1.4199572064851793 2316 100-101 -1.10123449445436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1223.0 3 425.0 4 380.0 5 373.0 6 502.0 7 576.0 8 724.0 9 879.0 10 1211.0 11 1616.0 12 2134.0 13 2705.0 14 3227.0 15 4036.0 16 5302.0 17 7217.0 18 6422.0 19 3533.0 20 3450.0 21 3521.0 22 3767.0 23 4200.0 24 4598.0 25 5188.0 26 6038.0 27 6942.0 28 8219.0 29 9827.0 30 11990.0 31 14912.0 32 18689.0 33 24380.0 34 32532.0 35 46400.0 36 67502.0 37 97926.0 38 89186.0 39 16135.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.102417515349686 19.38975940729639 15.22800972645786 21.279813350896067 2 14.82140926014615 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26.946923501720427 30.5069184041522 21.639273673763356 46-47 21.3018699790594 27.288332446728912 30.031946722636988 21.377850851574696 48-49 21.358651524411144 27.494914445870105 29.478159874181415 21.66827415553734 50-51 20.995705684947115 27.65189401692238 29.584432335574462 21.767967962556046 52-53 21.32365435264495 28.045229507310403 28.960285155978465 21.670830984066175 54-55 20.96513984322729 27.642606879732607 29.190928075522777 22.20132520151733 56-57 21.26923778730569 28.008268109176885 28.830831049240818 21.891663054276602 58-59 21.73960057077868 27.47992330402847 28.95147415758975 21.829001967603094 60-61 21.540552909077217 27.324432927382652 29.590213157899818 21.544801005640313 62-63 21.266324827253953 27.44279853905649 29.63682056520324 21.654056068486312 64-65 21.042910905274702 27.6653980501538 29.43576494486249 21.85592609970901 66-67 21.120930623263845 27.679630933104693 29.029451777698608 22.169986665932857 68-69 20.86622688439494 27.396337035949887 29.42382857760818 22.313607502046995 70-71 21.158354996079463 27.489735141544962 29.142251818318766 22.20965804405681 72-73 21.128287822023097 27.314703951179947 29.25939515661813 22.297613070178826 74-75 20.76074550858308 28.125066383943487 28.91974253651358 22.19444557095985 76-77 21.008290560735478 27.74030313418927 28.844391438076705 22.407014866998544 78-79 21.10739240095635 27.761769942951165 28.67910900305522 22.45172865303726 80-81 21.016944568668343 28.031028177843115 28.51078461958994 22.441242633898604 82-83 21.01711153304579 27.552388255450328 28.965697740674994 22.464802470828882 84-85 21.01915563786787 27.65883691153722 28.456328660457192 22.865678790137714 86-87 21.165347396960822 27.508269976845995 28.481162991443256 22.84521963474993 88-89 21.2919646641813 27.35619598267004 28.33894809411942 23.01289125902924 90-91 21.321991475259132 27.16271362548693 28.710261169168017 22.805033730085924 92-93 21.307074158379795 27.475945775505494 28.467666467401852 22.74931359871286 94-95 21.257297124020695 27.34941806461829 28.3887332212011 23.004551590159913 96-97 21.23623856090182 27.646865129447573 28.23474086827547 22.882155441375136 98-99 21.54402759910218 27.936461025539987 27.494601099482708 23.024910275875126 100-101 21.62690785317861 27.53065218105215 27.611786046134263 23.230653919634975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 455.0 1 324.5 2 216.5 3 242.0 4 241.5 5 249.0 6 368.5 7 729.5 8 1262.0 9 1515.0 10 1266.5 11 1052.5 12 1104.0 13 1160.0 14 1234.0 15 1277.0 16 1294.0 17 1381.5 18 1416.0 19 1443.0 20 1553.0 21 1775.5 22 2018.5 23 2314.5 24 2654.0 25 3138.5 26 3822.5 27 4506.5 28 4988.0 29 5422.0 30 6282.5 31 7330.0 32 8468.5 33 9766.0 34 11277.0 35 12247.5 36 13400.0 37 14947.5 38 15384.5 39 15228.0 40 15381.0 41 16020.0 42 16508.0 43 17212.0 44 18044.0 45 18575.5 46 18625.5 47 19572.0 48 22626.0 49 22234.5 50 18932.5 51 17125.0 52 15748.5 53 15234.5 54 14808.0 55 13599.0 56 12346.0 57 10962.5 58 9718.0 59 8655.5 60 7478.5 61 6171.5 62 4862.5 63 3704.0 64 2721.0 65 2017.0 66 1460.0 67 1039.0 68 721.0 69 439.5 70 255.0 71 174.5 72 114.0 73 82.0 74 67.0 75 48.0 76 30.0 77 15.5 78 8.0 79 5.0 80 4.5 81 2.0 82 2.0 83 3.0 84 2.5 85 1.0 86 0.5 87 2.0 88 2.5 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025294751435621963 2 0.011971561748156959 3 0.014288638215542175 4 0.029928904370392393 5 0.06371960285309348 6 0.050010233754397615 7 0.02046750879523609 8 0.05580292492286067 9 0.03707322347816348 10-11 0.02857727643108435 12-13 0.04344518376347283 14-15 0.024425847760352504 16-17 0.022977674968236744 18-19 0.0194055154143512 20-21 0.01612299041888881 22-23 0.007627043371809676 24-25 0.0025101661730006527 26-27 0.0 28-29 0.0030894352898469574 30-31 0.0 32-33 3.861794112308697E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 9.654485280771742E-5 48-49 0.0010619933808848916 50-51 0.0 52-53 3.861794112308697E-4 54-55 0.0020274419089620654 56-57 9.654485280771742E-5 58-59 9.654485280771742E-4 60-61 0.0028963455842315224 62-63 0.0018343522033466308 64-65 0.0013516279393080437 66-67 0.009171761016733154 68-69 0.011971561748156959 70-71 0.02085368820646696 72-73 0.014288638215542175 74-75 0.014095548509926741 76-77 0.02635674481650685 78-79 0.017764252916620003 80-81 0.0454726256724349 82-83 0.03340451907147022 84-85 0.01602644556608109 86-87 0.011006113220079786 88-89 0.012068106600964676 90-91 0.020660598500851526 92-93 0.030411628634430986 94-95 0.010233754397618046 96-97 0.018826246297504894 98-99 0.03784558230062523 100-101 0.044603721997165446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 517894.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.58102192994216 #Duplication Level Percentage of deduplicated Percentage of total 1 83.02602261560352 51.958533420629585 2 9.865378539298113 12.347709414299922 3 2.863904748173791 5.376782575521886 4 1.2853580860391023 3.2175609028096614 5 0.7062289092923191 2.2098263429990874 6 0.45070314201560147 1.6923277928623317 7 0.30399835942271247 1.3317169598389444 8 0.22164615334331314 1.1096674226452161 9 0.1771053864322242 0.9975092465003315 >10 1.0108258955315081 11.468377964886715 >50 0.064462106584749 2.736242339666946 >100 0.020973315973219504 2.4532758765642706 >500 0.0018505867035193604 0.7668658581896005 >1k 0.0015421555862661336 2.33360388258552 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4377 0.8451536414787582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3093 0.5972264594685399 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2143 0.41379123913387683 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1375 0.26549834522122284 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1102 0.21278485558820914 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 861 0.16625023653488938 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 737 0.14230711303857546 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 631 0.12183960424333937 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.11971561748156957 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 609 0.1175916307197998 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.65448528077174E-5 0.0 14-15 0.0 0.0 0.0 2.896345584231522E-4 0.0 16-17 0.0 0.0 0.0 4.82724264038587E-4 0.0 18-19 0.0 0.0 0.0 5.792691168463044E-4 0.0 20-21 0.0 0.0 0.0 7.723588224617392E-4 0.0 22-23 0.0 0.0 0.0 0.0013516279393080437 0.0 24-25 0.0 0.0 0.0 0.0016412624977311958 0.0 26-27 0.0 0.0 0.0 0.0027032558786160873 0.0 28-29 0.0 0.0 0.0 0.0054065117572321755 0.0 30-31 0.0 0.0 0.0 0.011971561748156959 0.0 32-33 0.0 0.0 0.0 0.017764252916620003 0.0 34-35 0.0 0.0 0.0 0.02172259188173642 0.0 36-37 0.0 0.0 0.0 0.027901462461430333 0.0 38-39 0.0 0.0 0.0 0.038424851417471526 0.0 40-41 0.0 0.0 0.0 0.06410578226432435 0.0 42-43 0.0 0.0 0.0 0.10957840793675926 0.0 44-45 0.0 0.0 0.0 0.14993415641038516 0.0 46-47 0.0 0.0 0.0 0.2389485106991006 0.0 48-49 0.0 0.0 0.0 0.3215909047025067 0.0 50-51 0.0 0.0 0.0 0.379228181828714 0.0 52-53 0.0 0.0 0.0 0.46476692141635156 0.0 54-55 0.0 0.0 0.0 0.5403615411647944 0.0 56-57 0.0 0.0 0.0 0.7067083225524915 0.0 58-59 0.0 0.0 0.0 0.9293407531270879 0.0 60-61 0.0 0.0 0.0 1.172247602791305 0.0 62-63 0.0 0.0 0.0 1.390825149547977 0.0 64-65 0.0 0.0 0.0 1.6727361197465118 0.0 66-67 0.0 0.0 0.0 2.037482573654068 0.0 68-69 0.0 0.0 0.0 2.467010623795603 0.0 70-71 0.0 0.0 0.0 2.8107102997910767 0.0 72-73 0.0 0.0 0.0 3.1047859214433844 0.0 74-75 0.0 0.0 0.0 3.422515032033582 0.0 76-77 0.0 0.0 0.0 3.790640555789409 0.0 78-79 0.0 0.0 0.0 4.163400232480005 0.0 80-81 0.0 0.0 0.0 4.607603100248314 0.0 82-83 0.0 0.0 0.0 5.0254492232001144 0.0 84-85 0.0 0.0 0.0 5.615434818708076 0.0 86-87 0.0 0.0 0.0 6.27039510015563 0.0 88-89 0.0 0.0 0.0 7.158704290839438 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCCGT 55 0.0025731577 34.559578 6 TACGCGA 60 0.003944799 31.673494 8 TTACGCT 65 0.0058239833 29.248373 4 GTATCAA 5410 0.0 28.549543 1 CTTTGCG 70 0.008362789 27.1592 2 TTTGCGT 90 8.9095044E-4 26.404778 3 GGTATCA 3780 0.0 26.025055 1 GAGTACT 2920 0.0 25.37973 12-13 TATCAAC 6360 0.0 24.212685 2 ATCAACG 6375 0.0 24.006605 3 TCAACGC 6380 0.0 23.987791 4 CAACGCA 6430 0.0 23.875177 5 AACGCAG 6500 0.0 23.613497 6 GTACTTT 3170 0.0 23.228315 14-15 GTACATG 3105 0.0 23.111517 1 AGTACTT 3115 0.0 23.104675 12-13 GGCGTGC 125 2.250291E-4 22.804914 8 AGACCCG 105 0.0021869363 22.63267 5 ACGCAGA 7065 0.0 21.586395 7 AGAGTAC 4945 0.0 21.472246 10-11 >>END_MODULE