FastQCFastQC Report
Thu 26 May 2016
SRR937121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517894
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47800.9229687928417784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38180.7372164960397302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.49971615813274534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19270.3720838627209429No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9670.18671774533012547No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8750.16895349241350546No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.16084372477765718No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.15601648213727132No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7040.13593515275326612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.12048797630403132No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5790.11179893955133675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG1652.6448106E-928.7841913
CGCGCGT350.00839248327.14200232-33
GGTCGAT350.00839643627.13938142-43
ATAAGGT700.00839262827.1393813
TATAGGG908.9374866E-426.3906042
CGGTTAC906.706614E-926.38550828-29
GTCGGGA1151.2798584E-424.7842222
GTATCAA63500.024.403911
GAGTACT35600.024.14718812-13
AGTACTT35600.024.08048412-13
TATTCAG1801.9455001E-723.7515455
GGTATCA47450.023.542211
GTGTAGC1852.5179725E-723.1252441
CGGGAGT1252.2555342E-422.797084
GTACTTT37850.022.52353514-15
GGTAGTT1703.141673E-622.3543977
GTACATG30050.021.8298191
GGTAAGG1553.8788465E-521.467521
GGACGCT1553.899133E-521.4530096
ACTTGAC1805.1567986E-621.1124848
Produced by FastQC (version 0.11.5)