##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937121_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 517894 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0287529880632 31.0 31.0 34.0 30.0 34.0 2 32.13722499198678 33.0 31.0 34.0 30.0 34.0 3 32.22957593638853 34.0 31.0 34.0 30.0 34.0 4 35.93150915052115 37.0 35.0 37.0 35.0 37.0 5 35.82973156669125 37.0 35.0 37.0 35.0 37.0 6 35.87500724086396 37.0 35.0 37.0 35.0 37.0 7 35.80627116745898 37.0 35.0 37.0 35.0 37.0 8 35.78673628194186 37.0 35.0 37.0 33.0 37.0 9 37.41256511950322 39.0 37.0 39.0 34.0 39.0 10-11 37.33303340065727 39.0 37.0 39.0 34.0 39.0 12-13 37.23255917234029 39.0 37.0 39.0 33.0 39.0 14-15 38.34372091586309 40.0 38.0 41.0 33.0 41.0 16-17 38.29325402495491 40.0 38.0 41.0 33.0 41.0 18-19 38.474989090431635 40.0 38.0 41.0 34.0 41.0 20-21 38.50743781546031 40.0 38.0 41.0 34.0 41.0 22-23 38.40815591607549 40.0 38.0 41.0 34.0 41.0 24-25 38.26799499511483 40.0 38.0 41.0 33.5 41.0 26-27 38.051306831127604 40.0 37.0 41.0 33.0 41.0 28-29 37.92817449130517 40.0 37.0 41.0 33.0 41.0 30-31 37.88171807358262 40.0 37.0 41.0 33.0 41.0 32-33 37.64171336219381 40.0 36.0 41.0 33.0 41.0 34-35 37.43736749218952 40.0 36.0 41.0 33.0 41.0 36-37 37.232634477325476 39.5 36.0 41.0 32.0 41.0 38-39 37.03956311523207 39.0 35.0 41.0 31.5 41.0 40-41 36.83196271823964 39.0 35.0 41.0 31.0 41.0 42-43 36.50841484937072 39.0 35.0 41.0 30.5 41.0 44-45 36.118667140380076 39.0 35.0 41.0 30.0 41.0 46-47 35.58690967649751 39.0 35.0 40.5 28.0 41.0 48-49 35.074147412404855 39.0 35.0 40.0 25.5 41.0 50-51 34.71861519924927 38.0 34.5 40.0 24.0 41.0 52-53 34.44915658416588 38.0 34.0 40.0 23.5 41.0 54-55 34.10591259987565 38.0 34.0 40.0 22.0 41.0 56-57 33.80974967850564 38.0 33.5 40.0 20.5 41.0 58-59 33.4510488632809 37.5 33.0 40.0 19.5 41.0 60-61 33.08567486783009 37.0 32.5 40.0 18.0 41.0 62-63 32.731510695238796 36.5 32.0 39.0 17.5 41.0 64-65 32.3528781951519 36.0 31.5 39.0 14.0 40.0 66-67 31.93687125164609 35.5 31.0 39.0 10.0 40.0 68-69 31.62023889058379 35.0 31.0 38.5 8.0 40.0 70-71 31.151901740510606 35.0 30.5 38.0 7.0 40.0 72-73 30.622219025514873 35.0 30.0 37.0 3.5 39.0 74-75 30.12547451795155 34.0 29.5 36.5 2.0 39.0 76-77 28.69245540593249 32.5 28.0 35.0 2.0 37.5 78-79 29.38356497661684 34.0 29.0 36.0 2.0 37.5 80-81 29.011858604270373 34.0 29.0 35.0 2.0 37.0 82-83 28.759386090589967 34.0 29.0 35.0 2.0 37.0 84-85 28.457742317926062 34.0 29.0 35.0 2.0 36.0 86-87 28.14471011442496 34.0 28.0 35.0 2.0 36.0 88-89 27.75161036814483 33.0 27.0 35.0 2.0 35.5 90-91 27.29718243501566 33.0 26.0 35.0 2.0 35.0 92-93 26.82321189278115 33.0 25.5 35.0 2.0 35.0 94-95 26.209990847547957 32.5 24.5 34.0 2.0 35.0 96-97 25.475773034636433 32.0 21.5 34.0 2.0 35.0 98-99 24.65646927749694 32.0 10.5 34.0 2.0 35.0 100-101 21.525061112891827 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3413268810721277 1101 2 0.3595832563604908 1101 3 0.4234002530947265 1101 4 0.2798729287433659 1101 5 0.3025651938674798 1101 6 0.35267358321808473 1101 7 0.3970437074407016 1101 8 0.31523399904095584 1101 9 0.4329557549740386 1101 10-11 0.5109214106047588 1101 12-13 0.5264687508526151 1101 14-15 0.6632839250846914 1101 16-17 0.7586512607810434 1101 18-19 0.5574794180344895 1101 20-21 0.6585335500150009 1101 22-23 0.6812824000220061 1101 24-25 0.6881517260917605 1101 26-27 0.5792549665376114 1101 28-29 0.7834869959819102 1101 30-31 0.8722505230069402 1101 32-33 0.9161933274003431 1101 34-35 1.0661801968546456 1101 36-37 1.0519392921445672 1101 38-39 1.2461564935983773 1101 40-41 1.231593582045896 1101 42-43 1.380956135981485 1101 44-45 1.497075554347262 1101 46-47 1.5518815927541496 1101 48-49 1.6798182224518783 1101 50-51 1.6864156141295226 1101 52-53 1.6700815080900568 1101 54-55 1.7677222078101735 1101 56-57 1.8024508395269407 1101 58-59 1.7262520646195583 1101 60-61 1.8314180002611593 1101 62-63 1.7806783929230896 1101 64-65 1.7988106632831027 1101 66-67 1.5606355839944719 1101 68-69 1.5126368201638165 1101 70-71 1.5103980959841579 1101 72-73 1.6099706992951894 1101 74-75 1.7747181689158964 1101 76-77 1.7899651036683935 1101 78-79 1.7972345031596255 1101 80-81 2.051140606719642 1101 82-83 1.9925985942529536 1101 84-85 1.9274441785987193 1101 86-87 1.6185793043203383 1101 88-89 1.4539951532936009 1101 90-91 1.7973212072612483 1101 92-93 2.051850625834941 1101 94-95 1.93334468368354 1101 96-97 1.888575306903956 1101 98-99 2.154357142946786 1101 100-101 2.233311345590348 1102 1 0.39216834966138947 1102 2 0.4273531539176183 1102 3 0.4172394877227532 1102 4 0.23537259830634838 1102 5 0.3033783498441025 1102 6 0.3146267543046264 1102 7 0.2952173591172098 1102 8 0.3994636520303416 1102 9 0.4556221082060361 1102 10-11 0.4849107686107246 1102 12-13 0.4734118018434046 1102 14-15 0.6819562040060632 1102 16-17 0.679102429286182 1102 18-19 0.5724695788471763 1102 20-21 0.5349811524615689 1102 22-23 0.5695407234126506 1102 24-25 0.4915742256740998 1102 26-27 0.4667075262787037 1102 28-29 0.6680284573266917 1102 30-31 0.7180524943927225 1102 32-33 0.6562857610579087 1102 34-35 0.613766823839299 1102 36-37 0.6077713207321764 1102 38-39 0.7225424440372592 1102 40-41 0.6488682676132669 1102 42-43 0.8772252285141988 1102 44-45 1.0484056557552037 1102 46-47 1.061793964109178 1102 48-49 1.1167912419365749 1102 50-51 1.2700398355326712 1102 52-53 1.3156231217167758 1102 54-55 1.4538698739785971 1102 56-57 1.3101691682105 1102 58-59 1.3180521997512926 1102 60-61 1.527538654866504 1102 62-63 1.5419136212055342 1102 64-65 1.5002042647804892 1102 66-67 1.4938266889551706 1102 68-69 1.3204917590514533 1102 70-71 1.396912569421307 1102 72-73 1.7352078081856455 1102 74-75 1.5515061141003095 1102 76-77 1.3843311155227305 1102 78-79 1.4672528509153864 1102 80-81 1.6198537652096547 1102 82-83 1.5891475602881684 1102 84-85 1.5955700906270707 1102 86-87 1.532273146659744 1102 88-89 1.6224736793227272 1102 90-91 1.836419964315379 1102 92-93 1.7462562605623226 1102 94-95 1.8978829515430355 1102 96-97 1.9951053929358622 1102 98-99 1.978138850485628 1102 100-101 1.610421300541507 1103 1 0.25519243956955506 1103 2 0.1944742220925768 1103 3 0.26300736735422703 1103 4 0.12338435235508882 1103 5 0.27607375353970554 1103 6 0.09532757841368067 1103 7 0.07082697151345485 1103 8 0.11410245070175051 1103 9 0.2745670424025164 1103 10-11 0.318364146426795 1103 12-13 0.37848607476417584 1103 14-15 0.4233553482288315 1103 16-17 0.34066425955948887 1103 18-19 0.41396740994532877 1103 20-21 0.3643198767495903 1103 22-23 0.34799494816832777 1103 24-25 0.36918777081598364 1103 26-27 0.39539852588837476 1103 28-29 0.3673671079677945 1103 30-31 0.5914652191078957 1103 32-33 0.5518072917355354 1103 34-35 0.7638025425964301 1103 36-37 0.46364871333654634 1103 38-39 0.6441283568540328 1103 40-41 0.8679059969313698 1103 42-43 0.9136973936600583 1103 44-45 0.8966860001282129 1103 46-47 1.031988318336154 1103 48-49 1.3172861492519203 1103 50-51 1.2655363262568429 1103 52-53 1.1861371035849047 1103 54-55 1.1260431209977355 1103 56-57 1.1364916828109983 1103 58-59 1.1780957987290748 1103 60-61 1.290286568817443 1103 62-63 1.442706062085474 1103 64-65 1.4376713998415802 1103 66-67 1.1092907013867652 1103 68-69 1.2197037369868333 1103 70-71 1.2100272450434382 1103 72-73 1.236193572235635 1103 74-75 1.2998074478460673 1103 76-77 1.1785575650782683 1103 78-79 1.3049206738387262 1103 80-81 1.3965787602384836 1103 82-83 1.2521933760382993 1103 84-85 1.3943759057880278 1103 86-87 1.252433991537231 1103 88-89 1.1847082742278303 1103 90-91 1.1504946275648287 1103 92-93 1.4376484083275045 1103 94-95 1.1975927826706432 1103 96-97 1.0771911474117495 1103 98-99 1.3272528780148463 1103 100-101 1.369997241565052 1104 1 0.11571647622885095 1104 2 0.19914065608386622 1104 3 0.0857307034021062 1104 4 0.17056187074290818 1104 5 0.14729439443111403 1104 6 -0.038904140411048616 1104 7 0.06560211661241055 1104 8 0.1328767685104637 1104 9 0.2161189871808915 1104 10-11 0.2584230908561551 1104 12-13 0.12782488889532573 1104 14-15 -0.05875837832589781 1104 16-17 0.10151985863395652 1104 18-19 0.04803108544072643 1104 20-21 0.038326158984823167 1104 22-23 0.07720023248472074 1104 24-25 0.037838641409692286 1104 26-27 -0.10182423493426285 1104 28-29 0.07999233256416716 1104 30-31 0.29122737316484404 1104 32-33 0.4439661314609751 1104 34-35 0.5574052623130825 1104 36-37 0.5728678289115763 1104 38-39 0.6386229988571088 1104 40-41 0.7238644255969717 1104 42-43 0.6987169771711237 1104 44-45 0.6758919307650899 1104 46-47 0.7885219722444461 1104 48-49 0.8598192085453604 1104 50-51 1.2357150985064251 1104 52-53 1.0984249488816218 1104 54-55 0.8855097773232714 1104 56-57 1.0925050487188912 1104 58-59 0.8029571629823806 1104 60-61 0.8574599507540697 1104 62-63 1.0138129505693279 1104 64-65 0.8302955903251608 1104 66-67 0.85890161644733 1104 68-69 1.0831892141652588 1104 70-71 1.1962922881530176 1104 72-73 1.1503499300903712 1104 74-75 1.0760034639265221 1104 76-77 1.2036974934659597 1104 78-79 1.4471648278698304 1104 80-81 1.5829213618077524 1104 82-83 1.6428818366321387 1104 84-85 1.4971376332734536 1104 86-87 1.365925285600131 1104 88-89 1.348178239714315 1104 90-91 1.31858319932579 1104 92-93 1.3831170553201737 1104 94-95 1.5436392401879964 1104 96-97 1.5125726704013722 1104 98-99 1.022742207263434 1104 100-101 0.5644233735954032 1105 1 0.19158134400367288 1105 2 0.1719585058614861 1105 3 0.1305787666117837 1105 4 0.10331048140539423 1105 5 0.12578554491023652 1105 6 0.15084085093405974 1105 7 0.0951446912142444 1105 8 0.1362973133129728 1105 9 0.21766775686144513 1105 10-11 0.23937819309980313 1105 12-13 0.21156197903040663 1105 14-15 0.3581381191356101 1105 16-17 0.12152963161910435 1105 18-19 0.3351829228447656 1105 20-21 0.20936996349199433 1105 22-23 0.4263401269696061 1105 24-25 0.47856714291290814 1105 26-27 0.37994252200828527 1105 28-29 0.22091795020854477 1105 30-31 0.4078903128456517 1105 32-33 0.37755952558558903 1105 34-35 0.5519141698092938 1105 36-37 0.69119548894421 1105 38-39 0.7774822098956093 1105 40-41 0.6537473982408954 1105 42-43 0.8797615270754662 1105 44-45 1.0014027348830012 1105 46-47 1.0557799873346028 1105 48-49 1.1231473240984897 1105 50-51 1.1366352499048702 1105 52-53 1.1675481133410912 1105 54-55 0.5277481222102622 1105 56-57 0.9859712283927351 1105 58-59 1.04105772534313 1105 60-61 1.105706196851095 1105 62-63 0.9264731074340311 1105 64-65 0.9754948184249734 1105 66-67 1.115550145116135 1105 68-69 1.1490450543521042 1105 70-71 0.7662353895021887 1105 72-73 0.6652578735395736 1105 74-75 0.9032659513997068 1105 76-77 0.7826102074570649 1105 78-79 0.8245925450495797 1105 80-81 0.6836410841887144 1105 82-83 0.7997216839085404 1105 84-85 1.0290364911593386 1105 86-87 1.2420734043004913 1105 88-89 0.7945055022017904 1105 90-91 0.8575384837776348 1105 92-93 1.123515925629352 1105 94-95 0.9959586677229701 1105 96-97 1.2886719241759828 1105 98-99 1.2385471119768283 1105 100-101 1.535311472915435 1106 1 0.11517859394822949 1106 2 0.09503299181022484 1106 3 0.19158374079041351 1106 4 0.1771152528149571 1106 5 0.14864458872736463 1106 6 0.1488606842832354 1106 7 0.09702980986583043 1106 8 0.1554019611601376 1106 9 0.071911650027495 1106 10-11 0.13016012130687926 1106 12-13 0.10115580724698958 1106 14-15 0.047204430956419685 1106 16-17 0.0509644128502984 1106 18-19 0.006641592806424512 1106 20-21 0.2526722478122423 1106 22-23 0.26300014027632557 1106 24-25 0.24756882209422315 1106 26-27 0.3414401616496363 1106 28-29 0.245226476014075 1106 30-31 0.28334575117533944 1106 32-33 0.05007164420549515 1106 34-35 -0.10158834896268587 1106 36-37 -0.11870059190994908 1106 38-39 0.2300849409414525 1106 40-41 -0.24002062127562596 1106 42-43 -0.28006960431254413 1106 44-45 -0.517844484963021 1106 46-47 -0.43755873205201823 1106 48-49 -0.14539495641984246 1106 50-51 -0.19905844207624313 1106 52-53 -0.17790383989529346 1106 54-55 -0.4872536203363893 1106 56-57 -0.40842252378544686 1106 58-59 -0.07353847907342725 1106 60-61 -0.2955785352719644 1106 62-63 -0.13485661407533556 1106 64-65 -0.3972121485933684 1106 66-67 -0.28060199504798433 1106 68-69 -0.06918515190205454 1106 70-71 -0.3328521497051149 1106 72-73 -0.3072890960133847 1106 74-75 -0.10649230985555747 1106 76-77 0.06318398054580854 1106 78-79 0.06690949844437455 1106 80-81 0.44063503279943106 1106 82-83 0.3197847324147034 1106 84-85 0.22123146628844736 1106 86-87 0.6469303570616347 1106 88-89 0.3099666434114319 1106 90-91 0.4378778051349208 1106 92-93 0.6046092944472683 1106 94-95 0.5641556071773941 1106 96-97 0.6587888332150449 1106 98-99 0.07261706730427164 1106 100-101 -0.3235818515467521 1107 1 0.003666152714426829 1107 2 -0.04186153208120302 1107 3 -0.07122196628774091 1107 4 -0.17137853066334685 1107 5 -0.19282182239848566 1107 6 -0.07484400226974941 1107 7 0.049959519761486604 1107 8 0.0486645293453023 1107 9 -0.572763763997763 1107 10-11 -0.04561837722703643 1107 12-13 -0.09604880559229656 1107 14-15 -0.21394681316696307 1107 16-17 -0.16909963616112123 1107 18-19 -0.24864941248458194 1107 20-21 -0.14811321257074184 1107 22-23 -0.19692418032910552 1107 24-25 0.09723383429793842 1107 26-27 -0.1914811940271406 1107 28-29 -0.19679770207821434 1107 30-31 -0.29096584480808474 1107 32-33 -0.23741177021290838 1107 34-35 -0.23547699213925455 1107 36-37 0.04953453839947741 1107 38-39 0.124166550193344 1107 40-41 0.049911725932261675 1107 42-43 -0.24878633076921375 1107 44-45 -0.2667019226749545 1107 46-47 -0.7566694172617829 1107 48-49 -0.852980914779792 1107 50-51 -0.8799600424824874 1107 52-53 -0.9617752832094624 1107 54-55 -0.762238468076653 1107 56-57 -1.1258083085086952 1107 58-59 -1.0270660889477874 1107 60-61 -1.0316630275484044 1107 62-63 -0.9357436355505939 1107 64-65 -0.9633210482935972 1107 66-67 -1.2163031761052032 1107 68-69 -1.1741043125487671 1107 70-71 -0.8825351808122939 1107 72-73 -1.0122786941918385 1107 74-75 -0.64031870591106 1107 76-77 -0.9332490956086303 1107 78-79 -1.1672256760932775 1107 80-81 -0.7942823259569352 1107 82-83 -0.6590635320372797 1107 84-85 -0.5631846393350912 1107 86-87 -0.6614631757901002 1107 88-89 -0.7466536655308573 1107 90-91 -0.9529890679300941 1107 92-93 -0.8491517421311023 1107 94-95 -0.9597522015601392 1107 96-97 -0.7267337968896292 1107 98-99 -1.9256260896076185 1107 100-101 -1.3120866101753492 1108 1 -0.14289863221410215 1108 2 -0.268076318107596 1108 3 -0.17850519312830926 1108 4 -0.11805529767474354 1108 5 -0.14737027111728196 1108 6 -0.31539688635731267 1108 7 -0.03969201834536307 1108 8 -0.25695872779629525 1108 9 -0.08850712741256217 1108 10-11 0.05504473236170071 1108 12-13 -0.10676944323144966 1108 14-15 -0.12399336258180682 1108 16-17 -0.15482192059561584 1108 18-19 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1.2304734400647348 2316 84-85 1.1109169291783303 2316 86-87 1.2456105289462265 2316 88-89 0.9570478208793496 2316 90-91 1.117435816184642 2316 92-93 1.5119655457261736 2316 94-95 1.2145546249797263 2316 96-97 1.3219038029598487 2316 98-99 1.0750067893072526 2316 100-101 0.7079303236118335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 12.0 5 19.0 6 41.0 7 94.0 8 212.0 9 444.0 10 701.0 11 1246.0 12 1769.0 13 2503.0 14 3210.0 15 4476.0 16 6073.0 17 8321.0 18 7351.0 19 4306.0 20 3680.0 21 3674.0 22 3698.0 23 4064.0 24 4679.0 25 5386.0 26 6273.0 27 7782.0 28 9434.0 29 11642.0 30 14250.0 31 17488.0 32 21447.0 33 27613.0 34 36799.0 35 50697.0 36 73467.0 37 102983.0 38 68043.0 39 4015.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36978691842947 20.218798770029515 15.603319066087737 20.80809524545328 2 15.40487569394159 22.22524740526189 39.562635771180304 22.80724112961622 3 18.884246136260067 27.113374989380024 31.20882346126219 22.79355541309771 4 12.520539957095384 18.448212275508645 38.162323105351874 30.86892466204409 5 12.854560971936344 38.876488238906035 33.58235469034204 14.686596098815588 6 29.213313921381594 38.27211746032972 17.493927328758392 15.020641289530289 7 26.268502821040602 35.18384070871646 21.28466442940061 17.262992040842335 8 26.479936048689503 33.80228386503802 20.072254167841297 19.645525918431183 9 26.835079833092934 16.84904024576505 21.20843494698712 35.107444974154895 10-11 24.38703398119655 27.61724154911694 27.03983253207372 20.95589193761279 12-13 26.247552587981325 25.6855649997876 27.959775552526196 20.10710685970488 14-15 22.96435177854928 26.035829725774 28.030060205370212 22.969758290306512 16-17 20.449551452613857 29.761978319887856 28.136259543458703 21.65221068403959 18-19 20.600280754058286 27.346487967545297 31.95846230191295 20.09476897648347 20-21 21.875021119451617 26.019067847746857 31.918322029398276 20.18758900340325 22-23 21.395986943034593 26.25614398577289 32.12444087436484 20.22342819682767 24-25 21.45427580220474 26.35899030296125 32.09465885599468 20.09207503883933 26-27 21.236946379744488 26.48745616609766 32.30693772882456 19.968659725333293 28-29 20.45018995254807 26.959842052163335 32.23091664775991 20.359051347528688 30-31 20.837352102069485 26.42134074814268 32.191568550781305 20.54973859900653 32-33 20.596952962076156 26.805920290414768 32.052888700084964 20.544238047424113 34-35 21.256666422086372 26.49171452073204 31.849857306707552 20.401761750474034 36-37 21.394301560220413 26.178468977596513 32.06258261424542 20.36464684793765 38-39 20.991033788885606 26.846299868876645 31.85708519928239 20.305581142955354 40-41 20.836780819074338 26.797994552892074 31.889717787288603 20.475506840744988 42-43 20.948205617365716 26.90415413192661 31.084449713647967 21.063190537059707 44-45 21.159349210456195 27.07194908610642 30.7083109671091 21.060390736328284 46-47 21.60639359867772 27.410488266881384 30.173993469693546 20.80912466474735 48-49 21.78613668290281 27.556034004180525 29.603139738644757 21.054689574271908 50-51 21.170562717061873 27.823119859187972 29.751850668190905 21.254466755559246 52-53 21.68194649870437 28.259547320494153 28.962683483492764 21.095822697308716 54-55 21.43448843163834 27.95030875997822 29.10231753424552 21.512885274137926 56-57 21.634535546982107 28.04656695206207 28.93036583905862 21.388531661897204 58-59 21.972362937024453 27.56475053172334 29.013509586467435 21.449376944784767 60-61 21.659948539953948 27.608073337806722 29.654792880555732 21.0771852416836 62-63 21.28274000324407 27.73467370063414 29.773417164219453 21.20916913190234 64-65 21.169913476335843 27.940906712390394 29.34602321328923 21.543156597984524 66-67 21.353780976315736 27.819234545754725 28.974724620106567 21.85225985782297 68-69 21.269112426492523 27.752541806698144 29.22302923693182 21.755316529877515 70-71 21.436253714599076 27.776125772124914 29.02040784801376 21.76721266526225 72-73 21.406407488791142 27.577554480260442 29.18724681112351 21.828791219824907 74-75 21.17877517288786 28.21892918138575 28.677421130019976 21.924874515706414 76-77 21.250197916948256 28.02812930831406 28.64466473834414 22.077008036393547 78-79 21.260342746801836 28.045184648805215 28.49374849143133 22.200724112961623 80-81 21.301507212626948 28.207980087916713 28.1843257996839 22.306186899772438 82-83 21.171296005716464 27.910931291370744 28.537714669203023 22.380058033709762 84-85 20.809138045902653 28.002645630364885 28.238531578591637 22.94968474514083 86-87 20.93989448804301 27.950627201384172 28.15106902304888 22.958409287523946 88-89 21.084125647818464 27.51616167077306 27.95158063843427 23.44813204297421 90-91 20.975984166445414 27.54497839781806 28.335014843957424 23.144022591779105 92-93 20.900110447738136 27.794501556309037 28.048125864079772 23.257262131873055 94-95 20.58461770169185 27.69514611098024 28.025426052435442 23.694810134892467 96-97 20.70897485780821 27.975793363063833 27.986799890707108 23.32843188842084 98-99 20.654904542970627 28.2299658026906 27.24971373511471 23.865415919224063 100-101 20.187915808620147 27.893390797107116 27.418098192381997 24.500595201890743 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 642.0 1 462.5 2 275.0 3 291.0 4 285.0 5 274.0 6 421.5 7 821.5 8 1361.0 9 1610.5 10 1383.0 11 1171.0 12 1212.5 13 1287.5 14 1330.0 15 1381.0 16 1463.0 17 1490.5 18 1573.5 19 1649.0 20 1735.5 21 1958.0 22 2174.0 23 2405.5 24 2693.5 25 3177.0 26 3868.5 27 4482.5 28 4951.0 29 5498.0 30 6330.5 31 7373.5 32 8516.0 33 9643.5 34 11092.5 35 12194.5 36 13375.0 37 14747.5 38 15164.0 39 15041.5 40 15130.0 41 15774.0 42 16474.5 43 17277.5 44 18232.5 45 18896.0 46 18651.5 47 19586.0 48 22781.0 49 22035.0 50 18656.5 51 16987.5 52 15668.0 53 15112.5 54 14574.0 55 13507.5 56 12087.5 57 10626.0 58 9594.5 59 8698.5 60 7537.0 61 6117.5 62 4805.0 63 3681.0 64 2617.0 65 1911.0 66 1391.5 67 1056.0 68 741.0 69 393.5 70 254.5 71 170.5 72 119.5 73 92.5 74 65.0 75 44.5 76 30.5 77 13.5 78 6.0 79 4.0 80 2.0 81 1.0 82 1.0 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030508173487238704 2 0.0036687044066932616 3 3.861794112308697E-4 4 1.9308970561543484E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 1.9308970561543484E-4 10-11 0.01226119630658011 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.792691168463044E-4 20-21 0.0012550830865003264 22-23 0.0018343522033466308 24-25 0.010716478661656632 26-27 0.005792691168463045 28-29 2.896345584231522E-4 30-31 0.0022205316145775004 32-33 0.0027032558786160873 34-35 0.0 36-37 0.009751030133579457 38-39 0.011778472042541524 40-41 8.689036752694568E-4 42-43 0.0 44-45 0.0 46-47 1.9308970561543484E-4 48-49 0.0031859801426546742 50-51 0.007820133077425111 52-53 0.0 54-55 0.0030894352898469574 56-57 0.0030894352898469574 58-59 4.827242640385871E-4 60-61 0.0031859801426546742 62-63 0.0050203323460013055 64-65 1.9308970561543484E-4 66-67 0.003572159553885544 68-69 8.689036752694568E-4 70-71 9.654485280771742E-4 72-73 0.0 74-75 9.654485280771742E-5 76-77 0.0 78-79 0.0036687044066932616 80-81 0.0033790698482701093 82-83 0.017667708063812286 84-85 0.011392292631310655 86-87 0.007337408813386523 88-89 3.861794112308697E-4 90-91 0.0012550830865003264 92-93 3.861794112308697E-4 94-95 0.0 96-97 0.003765249259500979 98-99 0.003282524995462392 100-101 4.827242640385871E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 517894.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.96948737865699 #Duplication Level Percentage of deduplicated Percentage of total 1 81.6164603653403 48.944972897699344 2 10.638576533974254 12.75979962362091 3 3.123581362852557 5.619587193473839 4 1.3923864774915586 3.3400281315257083 5 0.7768500405623747 2.329364935130725 6 0.49323854550713436 1.7747557637674338 7 0.33617345745401117 1.4112104939679464 8 0.24567392723443995 1.1786351582840675 9 0.18344245192151085 0.9900854824695243 >10 1.0886664348923938 11.923660015355686 >50 0.07060883435917446 2.847810045629322 >100 0.030811126798045253 3.3187880136865293 >500 0.0022466446623574626 1.037421991171512 >1k 0.00128379694991855 2.5238802542174463 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4780 0.9229687928417784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3818 0.7372164960397302 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2588 0.49971615813274534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1927 0.3720838627209429 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 967 0.18671774533012547 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 875 0.16895349241350546 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 833 0.16084372477765718 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 808 0.15601648213727132 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 704 0.13593515275326612 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 624 0.12048797630403132 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 579 0.11179893955133675 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.930897056154348E-4 0.0 14-15 0.0 0.0 0.0 4.82724264038587E-4 0.0 16-17 0.0 0.0 0.0 6.758139696540218E-4 0.0 18-19 0.0 0.0 0.0 9.65448528077174E-4 0.0 20-21 0.0 0.0 0.0 0.0011585382336926089 0.0 22-23 0.0 0.0 0.0 0.0018343522033466308 0.0 24-25 0.0 0.0 0.0 0.0022205316145775004 0.0 26-27 0.0 0.0 0.0 0.003282524995462392 0.0 28-29 0.0 0.0 0.0 0.005985780874078479 0.0 30-31 0.0 0.0 0.0 0.012937010276234133 0.0 32-33 0.0 0.0 0.0 0.01930897056154348 0.0 34-35 0.0 0.0 0.0 0.023363854379467613 0.0 36-37 0.0 0.0 0.0 0.029542724959161527 0.0 38-39 0.0 0.0 0.0 0.04035574847362588 0.0 40-41 0.0 0.0 0.0 0.06661594843732502 0.0 42-43 0.0 0.0 0.0 0.11324711234345253 0.0 44-45 0.0 0.0 0.0 0.1548579439035787 0.0 46-47 0.0 0.0 0.0 0.24425847760352504 0.0 48-49 0.0 0.0 0.0 0.32757668557658515 0.0 50-51 0.0 0.0 0.0 0.3858897766724465 0.0 52-53 0.0 0.0 0.0 0.47229741993535357 0.0 54-55 0.0 0.0 0.0 0.5483747639478349 0.0 56-57 0.0 0.0 0.0 0.7168455320973017 0.0 58-59 0.0 0.0 0.0 0.9401537766415521 0.0 60-61 0.0 0.0 0.0 1.183060626305769 0.0 62-63 0.0 0.0 0.0 1.3998038208590948 0.0 64-65 0.0 0.0 0.0 1.6826802395857068 0.0 66-67 0.0 0.0 0.0 2.0481990523157245 0.0 68-69 0.0 0.0 0.0 2.4744445774617976 0.0 70-71 0.0 0.0 0.0 2.816406446106732 0.0 72-73 0.0 0.0 0.0 3.1094200743781544 0.0 74-75 0.0 0.0 0.0 3.4237701151200826 0.0 76-77 0.0 0.0 0.0 3.7900612866725623 0.0 78-79 0.0 0.0 0.0 4.1611797008654285 0.0 80-81 0.0 0.0 0.0 4.607892734806736 0.0 82-83 0.0 0.0 0.0 5.026221582022576 0.0 84-85 0.0 0.0 0.0 5.619296612820384 0.0 86-87 0.0 0.0 0.0 6.276380881029709 0.0 88-89 0.0 0.0 0.0 7.167489872444941 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 165 2.6448106E-9 28.784191 3 CGCGCGT 35 0.008392483 27.142002 32-33 GGTCGAT 35 0.008396436 27.139381 42-43 ATAAGGT 70 0.008392628 27.139381 3 TATAGGG 90 8.9374866E-4 26.390604 2 CGGTTAC 90 6.706614E-9 26.385508 28-29 GTCGGGA 115 1.2798584E-4 24.784222 2 GTATCAA 6350 0.0 24.40391 1 GAGTACT 3560 0.0 24.147188 12-13 AGTACTT 3560 0.0 24.080484 12-13 TATTCAG 180 1.9455001E-7 23.751545 5 GGTATCA 4745 0.0 23.54221 1 GTGTAGC 185 2.5179725E-7 23.125244 1 CGGGAGT 125 2.2555342E-4 22.79708 4 GTACTTT 3785 0.0 22.523535 14-15 GGTAGTT 170 3.141673E-6 22.354397 7 GTACATG 3005 0.0 21.829819 1 GGTAAGG 155 3.8788465E-5 21.46752 1 GGACGCT 155 3.899133E-5 21.453009 6 ACTTGAC 180 5.1567986E-6 21.112484 8 >>END_MODULE