FastQCFastQC Report
Thu 26 May 2016
SRR937120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547774
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46750.8534541617528396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.5865557693501335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22070.40290338716331914No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15280.2789471570392169No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11300.2062894551402586No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9260.16904781899104374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7660.1398386925995027No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.1318061828418289No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6840.12486901532383793No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.10460518388970634No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5730.10460518388970634No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG352.7683133E-454.358324
TATACGT600.003923401731.7090195
GTATCAA51150.028.077341
GGTATCA36300.025.8079931
GAGTACT28650.024.54900712-13
CTAGACA1551.3920799E-624.548924
GTACATG33100.024.280261
TCAACGC61700.023.280694
TATCAAC61800.023.243022
ATCAACG61900.023.1286323
CAACGCA61850.023.0704255
TACATGG34600.022.8194962
AACGCAG63200.022.5734946
AGTACTT30650.022.48196812-13
TAGACAG1703.107718E-622.3828375
ACATGGG34400.022.1225723
GTACTTT31150.022.04180114-15
ATACAGT2405.83168E-921.7959616
AGAGTAC50100.021.48460210-11
ACGCAGA68850.020.5753927
Produced by FastQC (version 0.11.5)