##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937120_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547774 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65106960169705 33.0 31.0 34.0 30.0 34.0 2 31.716310011062955 33.0 31.0 34.0 30.0 34.0 3 31.71157448144673 33.0 31.0 34.0 30.0 34.0 4 35.34240946083604 37.0 35.0 37.0 33.0 37.0 5 35.346148229014155 37.0 35.0 37.0 33.0 37.0 6 35.410968757188186 37.0 35.0 37.0 33.0 37.0 7 35.35752700931406 37.0 35.0 37.0 33.0 37.0 8 35.31957887742025 37.0 35.0 37.0 33.0 37.0 9 36.90887665351039 39.0 37.0 39.0 33.0 39.0 10-11 36.793287925312264 39.0 37.0 39.0 33.0 39.0 12-13 36.705647767144846 39.0 37.0 39.0 32.0 39.0 14-15 37.79174896946551 40.0 37.5 41.0 32.5 41.0 16-17 37.756617692698086 40.0 37.0 41.0 32.0 41.0 18-19 37.739120513204355 40.0 37.0 41.0 32.0 41.0 20-21 37.71316090212387 40.0 37.0 41.0 32.0 41.0 22-23 37.60692639665264 40.0 37.0 41.0 32.0 41.0 24-25 37.55474885628014 40.0 37.0 41.0 32.0 41.0 26-27 37.5265702643791 40.0 37.0 41.0 32.0 41.0 28-29 37.42963065059678 40.0 37.0 41.0 32.0 41.0 30-31 37.22908900385926 39.5 36.5 41.0 31.5 41.0 32-33 36.95660254046376 39.0 36.0 41.0 31.0 41.0 34-35 36.763090252549404 39.0 35.0 41.0 30.5 41.0 36-37 36.56631567033119 39.0 35.0 41.0 30.0 41.0 38-39 36.374848934049446 39.0 35.0 41.0 30.0 41.0 40-41 36.159647044218964 39.0 35.0 40.5 30.0 41.0 42-43 35.87351718044303 39.0 35.0 40.0 29.0 41.0 44-45 35.54113557781129 38.0 35.0 40.0 27.5 41.0 46-47 35.07476988685114 38.0 35.0 40.0 25.5 41.0 48-49 34.723508235148074 38.5 34.0 40.0 24.0 41.0 50-51 33.978481088916226 38.0 33.5 39.5 23.0 40.5 52-53 34.143822999996345 38.0 33.5 39.5 23.0 40.5 54-55 34.52222084290237 38.0 34.0 40.0 23.0 41.0 56-57 34.392139824088034 38.0 34.0 40.0 22.5 41.0 58-59 34.17235118862889 38.0 34.0 40.0 22.0 41.0 60-61 33.861516793422105 38.0 34.0 40.0 20.5 41.0 62-63 33.56221270085838 37.5 33.0 40.0 19.5 41.0 64-65 33.221657106763004 37.0 33.0 40.0 18.0 41.0 66-67 32.83372704801616 36.5 32.5 39.5 14.5 41.0 68-69 32.43561213201065 36.0 32.0 39.0 12.0 41.0 70-71 31.972082464666087 35.5 31.5 39.0 8.0 40.5 72-73 31.589140777035784 35.0 31.0 38.0 7.0 40.0 74-75 31.213702183747312 35.0 31.0 37.0 4.0 39.5 76-77 30.771947007342444 35.0 31.0 37.0 2.0 39.0 78-79 30.308096039607577 35.0 30.5 36.0 2.0 39.0 80-81 29.866519769101856 34.5 30.0 36.0 2.0 37.5 82-83 29.446109161807605 34.0 29.5 35.0 2.0 37.0 84-85 29.093796894339636 34.0 29.0 35.0 2.0 37.0 86-87 28.753897592802872 34.0 29.0 35.0 2.0 36.0 88-89 28.365150591302253 34.0 29.0 35.0 2.0 36.0 90-91 27.97057271794572 34.0 27.5 35.0 2.0 35.5 92-93 27.575762084363262 34.0 27.0 35.0 2.0 35.0 94-95 27.200188398865222 34.0 26.0 35.0 2.0 35.0 96-97 26.70514664807019 33.5 25.0 35.0 2.0 35.0 98-99 26.136356417062512 33.0 24.5 35.0 2.0 35.0 100-101 24.16720581845798 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3166882186230531 1101 2 0.33935361605871606 1101 3 0.4857100147890847 1101 4 0.4871627494920858 1101 5 0.388917624612688 1101 6 0.34116685876169583 1101 7 0.39682318340053513 1101 8 0.57465970910647 1101 9 0.3709730673368412 1101 10-11 0.444631207405358 1101 12-13 0.6175123839210883 1101 14-15 0.7600568036690376 1101 16-17 0.7563438856968432 1101 18-19 0.7467106909911578 1101 20-21 0.8142760378700515 1101 22-23 0.7447850019254076 1101 24-25 0.691294831553229 1101 26-27 0.7868833814765637 1101 28-29 0.7849502014959953 1101 30-31 0.7656265876833501 1101 32-33 0.7299740190539481 1101 34-35 0.7983718651970051 1101 36-37 0.895862245758444 1101 38-39 0.9764555788282436 1101 40-41 1.0719283227258884 1101 42-43 1.093194024836528 1101 44-45 1.2799981606896225 1101 46-47 1.1765721634369868 1101 48-49 1.2289865948606504 1101 50-51 1.52425479873272 1101 52-53 1.5866133399074585 1101 54-55 1.5977291850958792 1101 56-57 1.64330623331643 1101 58-59 1.553926070164593 1101 60-61 1.6075796734192096 1101 62-63 1.7574474408240803 1101 64-65 1.697241312647634 1101 66-67 1.6454621643061564 1101 68-69 1.6242650053995504 1101 70-71 1.5949784582655262 1101 72-73 1.6192798424462858 1101 74-75 1.597592587522282 1101 76-77 1.6463185713268267 1101 78-79 1.6184265840026129 1101 80-81 1.6536416121677355 1101 82-83 1.7606522615119466 1101 84-85 1.8294325931154347 1101 86-87 1.8813815613710965 1101 88-89 1.9269483799343874 1101 90-91 2.030843672072667 1101 92-93 2.1265007376378016 1101 94-95 2.1895985006379632 1101 96-97 2.280944399171709 1101 98-99 2.2031765664008702 1101 100-101 2.506814274756856 1102 1 0.23563440087000842 1102 2 0.10798766286439587 1102 3 0.310540984980463 1102 4 0.35731398778962387 1102 5 0.2507124974796895 1102 6 0.3229843943760784 1102 7 0.21288070162062667 1102 8 0.00761644500183678 1102 9 0.20225685761862167 1102 10-11 0.31365610297692825 1102 12-13 0.28294678112127514 1102 14-15 0.3035027956659988 1102 16-17 0.5640654224150978 1102 18-19 0.23126333170298352 1102 20-21 0.20927319457333482 1102 22-23 0.33442724829706094 1102 24-25 0.27309606002466325 1102 26-27 0.34650062356237044 1102 28-29 0.22013714825526876 1102 30-31 0.3476593566780366 1102 32-33 0.32193219332295087 1102 34-35 0.2585186712967129 1102 36-37 0.5013156261455478 1102 38-39 0.5761784619311143 1102 40-41 0.3543784918602384 1102 42-43 0.6306340751796071 1102 44-45 0.6153137875320098 1102 46-47 0.7480115614943443 1102 48-49 0.9533504072197942 1102 50-51 0.8865571791574567 1102 52-53 1.2108507719087669 1102 54-55 0.9516020270127683 1102 56-57 1.2332202654038653 1102 58-59 0.9079740048022202 1102 60-61 0.84960851197431 1102 62-63 0.7514958563133618 1102 64-65 1.0485374169129997 1102 66-67 0.7085681941858617 1102 68-69 0.8987178798470836 1102 70-71 0.8845562077961766 1102 72-73 0.9061230095443271 1102 74-75 0.8215492439725942 1102 76-77 0.8620395979241913 1102 78-79 0.7518754611094742 1102 80-81 1.011808668393929 1102 82-83 1.0939305408156734 1102 84-85 1.0493851142415522 1102 86-87 1.1194632350693468 1102 88-89 1.2699189559946653 1102 90-91 0.9368350836440484 1102 92-93 1.062024280552766 1102 94-95 1.0644448371755821 1102 96-97 1.0317994788467395 1102 98-99 1.2470119437021658 1102 100-101 1.0545110998815659 1103 1 0.31417735247515566 1103 2 0.4144422762409654 1103 3 0.3990780985914597 1103 4 0.3489048563198054 1103 5 0.2637315269207292 1103 6 0.3045901755027245 1103 7 0.37643225897362953 1103 8 0.3868909607961797 1103 9 0.3134782083429215 1103 10-11 0.3737779900102325 1103 12-13 0.2750378871035579 1103 14-15 0.724970431096871 1103 16-17 0.7800451164800037 1103 18-19 0.6737723231214403 1103 20-21 0.5157092806368624 1103 22-23 0.3468405480342085 1103 24-25 0.5238341276788461 1103 26-27 0.674781924134372 1103 28-29 0.7935366908953156 1103 30-31 0.9215073359153436 1103 32-33 0.6418780935688702 1103 34-35 0.7674004383032766 1103 36-37 0.8079657215801248 1103 38-39 0.7917845848677771 1103 40-41 0.6357872028734732 1103 42-43 0.7394071738235581 1103 44-45 0.9202778974798989 1103 46-47 0.8815386627941351 1103 48-49 0.939608812570178 1103 50-51 0.9226986733814826 1103 52-53 0.9419272012342503 1103 54-55 1.1509109204255026 1103 56-57 1.2010644121341372 1103 58-59 1.2931681861672217 1103 60-61 1.4546315312154974 1103 62-63 1.472549765500787 1103 64-65 1.4319427672985157 1103 66-67 1.3691179487178609 1103 68-69 1.2122994590093796 1103 70-71 1.3146541681280688 1103 72-73 1.178938450282633 1103 74-75 1.2188512134637826 1103 76-77 1.131636579711465 1103 78-79 1.376648217943405 1103 80-81 1.2850528840911757 1103 82-83 1.2861948960802998 1103 84-85 1.227954760301813 1103 86-87 1.3071354634589376 1103 88-89 1.3870561912053319 1103 90-91 1.6827220201490576 1103 92-93 1.5262737504447514 1103 94-95 1.5801828443324197 1103 96-97 1.7754752372566251 1103 98-99 1.8273240646484652 1103 100-101 1.7800176139335875 1104 1 0.14774586925157251 1104 2 0.14320986955312165 1104 3 0.06299527766263324 1104 4 0.18050783031102213 1104 5 0.12210678425913102 1104 6 0.058938228820061056 1104 7 -0.04984511660637736 1104 8 0.06493505257907373 1104 9 0.10258228440147832 1104 10-11 0.27535826368649907 1104 12-13 0.11338505876701532 1104 14-15 -0.02386955781428668 1104 16-17 0.43933531694263905 1104 18-19 0.28858388096747234 1104 20-21 0.23961982458094866 1104 22-23 0.07087038792767686 1104 24-25 0.25774508063761203 1104 26-27 0.1512329037245408 1104 28-29 0.2249971914383977 1104 30-31 0.333648141492489 1104 32-33 0.15607715192617633 1104 34-35 0.022755122203093947 1104 36-37 0.08802943697861565 1104 38-39 0.5209782351420458 1104 40-41 0.22860570033531502 1104 42-43 0.06637976075054297 1104 44-45 -0.03271268154855278 1104 46-47 0.018294426492715843 1104 48-49 -0.03753919620999824 1104 50-51 -0.12665406088168396 1104 52-53 0.11443823326919045 1104 54-55 0.23533495958184858 1104 56-57 0.39056489159023755 1104 58-59 0.324306701262131 1104 60-61 0.3741902725409716 1104 62-63 0.28526762262740846 1104 64-65 0.24528664412147094 1104 66-67 0.21530002387658342 1104 68-69 0.2465692087378386 1104 70-71 0.38706680940855165 1104 72-73 0.2712087544532231 1104 74-75 0.4131405731740152 1104 76-77 0.45677257709376917 1104 78-79 0.5838476716815713 1104 80-81 0.44107494361644584 1104 82-83 0.3215797788975969 1104 84-85 0.4092613122625792 1104 86-87 0.44206486297993663 1104 88-89 0.31007110660746307 1104 90-91 0.5289739686495629 1104 92-93 0.6039433665617473 1104 94-95 0.5278090821699095 1104 96-97 0.5615247689348131 1104 98-99 0.3198504574570791 1104 100-101 0.1676959329741301 1105 1 0.12776870030180376 1105 2 0.40794251464609843 1105 3 0.3891649553808527 1105 4 0.0700336724127979 1105 5 0.4316412293653471 1105 6 0.3983929306928644 1105 7 0.24061382059169034 1105 8 0.3545419614534069 1105 9 0.38955038710811607 1105 10-11 0.48017594616946013 1105 12-13 0.3384818425668996 1105 14-15 0.4116658137693321 1105 16-17 0.5565636605334063 1105 18-19 0.4982159370855399 1105 20-21 0.2878173297096609 1105 22-23 0.26864849659024514 1105 24-25 0.3000611086966458 1105 26-27 0.39114505381387943 1105 28-29 0.5933174548226816 1105 30-31 0.6291034389579124 1105 32-33 0.525960845923926 1105 34-35 0.37401827487333605 1105 36-37 0.3806349892253209 1105 38-39 0.9103138184919217 1105 40-41 0.8847957274678677 1105 42-43 0.9597893630270278 1105 44-45 1.0962579358824556 1105 46-47 1.015366096137349 1105 48-49 1.044937972739767 1105 50-51 0.8946755996305242 1105 52-53 1.0282427465376713 1105 54-55 1.0325604906519317 1105 56-57 1.2585087735305436 1105 58-59 1.3088626538794301 1105 60-61 1.3634447891183044 1105 62-63 1.090872810900855 1105 64-65 1.2876440327787435 1105 66-67 1.2498485315861991 1105 68-69 1.1553029126621368 1105 70-71 1.0215342503456135 1105 72-73 1.0603069279692008 1105 74-75 1.2926488783821455 1105 76-77 1.155291040464828 1105 78-79 0.8933655725484932 1105 80-81 0.7970407963065362 1105 82-83 1.0773495823520314 1105 84-85 0.7974305267421009 1105 86-87 1.0929276450592162 1105 88-89 1.3268717816124251 1105 90-91 1.2228683171374222 1105 92-93 1.2297581703470684 1105 94-95 1.4702144491211406 1105 96-97 1.0955107725083693 1105 98-99 0.8738839429974128 1105 100-101 1.1340242699471936 1106 1 0.1262937240370512 1106 2 0.29687849728545856 1106 3 0.31612819965048544 1106 4 0.16878926715035192 1106 5 0.08515743715050661 1106 6 0.0704056799129944 1106 7 0.3507623354431786 1106 8 0.3459294563672799 1106 9 0.08779058784051585 1106 10-11 0.40973311421502956 1106 12-13 0.39389517499603244 1106 14-15 0.43094222771209445 1106 16-17 0.2541341881341097 1106 18-19 0.44955867409895944 1106 20-21 0.31698625687634774 1106 22-23 -0.04379216528415952 1106 24-25 0.05605425938157538 1106 26-27 -0.019720604669778652 1106 28-29 -0.020721945692706356 1106 30-31 0.3431784423486661 1106 32-33 0.6038039898273624 1106 34-35 0.3619895891280507 1106 36-37 0.1446133562401073 1106 38-39 0.02136088210239251 1106 40-41 0.3862622268575322 1106 42-43 0.41622670196640144 1106 44-45 -0.13722911145242023 1106 46-47 0.32933259552701344 1106 48-49 0.20420997870748892 1106 50-51 0.43850952069387006 1106 52-53 0.5694234056994816 1106 54-55 0.6890944659672726 1106 56-57 0.5036430472318827 1106 58-59 0.5175972259667816 1106 60-61 0.7984441109682905 1106 62-63 0.7334368960764976 1106 64-65 0.45142573629156146 1106 66-67 0.42414156020413074 1106 68-69 0.620666401065769 1106 70-71 0.5828983490842568 1106 72-73 0.717578391416918 1106 74-75 1.3901159881068175 1106 76-77 1.1751862662887795 1106 78-79 1.3372317057371532 1106 80-81 1.4857190819433193 1106 82-83 1.4353873553074088 1106 84-85 0.9635433709032277 1106 86-87 1.3222186392271276 1106 88-89 1.1398888709925963 1106 90-91 1.0255181882889168 1106 92-93 1.3161629580861174 1106 94-95 1.6147536461915308 1106 96-97 1.2889784925680452 1106 98-99 1.2556315355648877 1106 100-101 1.3558386602903383 1107 1 -0.10336155046049811 1107 2 -0.036597375218732964 1107 3 -0.11473756134422075 1107 4 0.030183629177919613 1107 5 -0.0221827819212308 1107 6 0.0016332711106556985 1107 7 -0.05465196013920348 1107 8 -0.5888999900325089 1107 9 -0.19468367287458932 1107 10-11 -0.08154184384556373 1107 12-13 -0.13517048073897797 1107 14-15 -0.3816543973989255 1107 16-17 -0.21566532503980085 1107 18-19 -0.3099804857970909 1107 20-21 0.06085981366724269 1107 22-23 -0.11221096729725843 1107 24-25 -0.054410856897497695 1107 26-27 0.21489749999783925 1107 28-29 0.20620315545527745 1107 30-31 -0.13202385362844637 1107 32-33 0.07081987746263252 1107 34-35 0.19093847238845996 1107 36-37 -0.10294428988995463 1107 38-39 -0.09879963893309451 1107 40-41 -0.05940567880346492 1107 42-43 0.14593542865302567 1107 44-45 -0.07320572406384684 1107 46-47 0.30309990581285007 1107 48-49 0.18823599758844267 1107 50-51 -0.14140561367671012 1107 52-53 0.15778809765039625 1107 54-55 0.11059375875369426 1107 56-57 0.15136022758779433 1107 58-59 0.13862531714799076 1107 60-61 0.03574716021009294 1107 62-63 0.23105621780933205 1107 64-65 0.1222507091542937 1107 66-67 0.1875703947819396 1107 68-69 0.08570044599850135 1107 70-71 0.17763207494853717 1107 72-73 0.5018289094919801 1107 74-75 0.6098620490916531 1107 76-77 0.6773778060407594 1107 78-79 0.5086775463344999 1107 80-81 0.4009069047123113 1107 82-83 0.6344679776553583 1107 84-85 0.42382428271534067 1107 86-87 0.6002974853177143 1107 88-89 0.5833570134879089 1107 90-91 0.47813899023622497 1107 92-93 0.8035276829306959 1107 94-95 0.7051988618094391 1107 96-97 0.5246368590691475 1107 98-99 0.8706071942603266 1107 100-101 0.6899025068743327 1108 1 -0.25667372606259065 1108 2 -0.23065764291002822 1108 3 -0.019485313777536817 1108 4 0.05409752641078569 1108 5 0.056553698182362666 1108 6 0.07041358805204823 1108 7 0.20427196755654364 1108 8 0.09486976573367656 1108 9 -0.11628684865712557 1108 10-11 -0.41496716731472105 1108 12-13 -0.02944727905768474 1108 14-15 0.13528063279860447 1108 16-17 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-2.4807036724000113 2316 84-85 -2.1459431591451263 2316 86-87 -2.5346231496029183 2316 88-89 -2.570574326489943 2316 90-91 -3.2688156055555844 2316 92-93 -3.3049994488522536 2316 94-95 -3.66634045159034 2316 96-97 -3.6759906787676933 2316 98-99 -4.168521635566179 2316 100-101 -4.471965341040029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1257.0 3 435.0 4 385.0 5 450.0 6 507.0 7 529.0 8 739.0 9 957.0 10 1271.0 11 1646.0 12 2247.0 13 2888.0 14 3313.0 15 4228.0 16 5660.0 17 7570.0 18 6631.0 19 3806.0 20 3798.0 21 3815.0 22 3981.0 23 4537.0 24 4969.0 25 5551.0 26 6367.0 27 7378.0 28 8847.0 29 10523.0 30 12970.0 31 16013.0 32 20067.0 33 25722.0 34 34604.0 35 48636.0 36 70874.0 37 102630.0 38 94532.0 39 17436.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21412512852731 19.397168107329207 15.200283444951959 21.188423319191525 2 14.810127738059368 21.351339012609454 40.79126377589686 23.04726947343432 3 18.648298732152348 26.86292161914901 31.048115393675552 23.44066425502309 4 12.01681224861085 17.42690763492359 38.986369730646004 31.56991038581955 5 12.689817260052157 38.019193268649104 34.39528045163212 14.895709019666612 6 28.935427255109875 37.39167794069253 18.56557826611576 15.10731653808183 7 26.211552339974602 34.7366786337435 21.85937314441795 17.192395881863938 8 25.993124836664617 33.28989906558453 20.872937405311166 19.844038692439696 9 26.622798762537712 16.78340207108647 21.465000392874174 35.12879877350164 10-11 23.91204039994262 27.22939320594596 27.86614732237082 20.9924190717406 12-13 25.926555329274898 25.308288358910964 28.21972552126018 20.545430790553958 14-15 22.806878111122984 25.71170435413338 28.01698425357257 23.464433281171065 16-17 20.61760093239327 29.40180870410306 28.067430447280227 21.913159916223442 18-19 20.504678839077897 27.488681996061075 31.62710693915134 20.37953222570968 20-21 21.900909862337762 26.209168774394147 31.276483607223724 20.61343775604436 22-23 21.401652989159963 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29.974030952913417 21.374031865709448 48-49 21.36834053522171 27.42431627699795 29.601678067374625 21.605665120405714 50-51 20.98146192387422 27.59096290467893 29.61774631251204 21.809828858934814 52-53 21.409369200617444 28.01084088023274 28.895564787404275 21.684225131745542 54-55 20.96865925209807 27.704487174220453 29.108526388725327 22.21832718495615 56-57 21.25084098703807 27.98248008332732 28.78215948126733 21.984519448367276 58-59 21.686368656948034 27.422614537786743 28.836867520470292 22.05414928479493 60-61 21.44270046507807 27.361377982044978 29.65701062970384 21.53891092317312 62-63 21.20521654351958 27.43660526656917 29.655443758569632 21.702734431341618 64-65 20.974024136549225 27.742426635095573 29.40045658807668 21.883092640278516 66-67 21.105450561490002 27.722724367753127 28.919748014242675 22.252077056514196 68-69 20.916135100704544 27.48103941883136 29.326277242279925 22.27654823818417 70-71 21.149553367553843 27.547448988911828 29.07842098533146 22.224576658202874 72-73 21.10211078615009 27.387915831443816 29.311575549786582 22.198397832619516 74-75 20.79183516975069 28.136348283322533 28.80229681494938 22.269519731977397 76-77 20.962408266638914 27.75565282015605 28.8558140554043 22.426124857800737 78-79 21.003538085781457 27.848341664954688 28.643155515989864 22.504964733273987 80-81 21.091944257161078 27.932172359167474 28.478400289421323 22.497483094250125 82-83 21.096824501140432 27.592818832759008 28.852773653713758 22.4575830123868 84-85 21.02745891387662 27.6651433016062 28.453817937486647 22.853579847030534 86-87 21.23911098124441 27.54515404423179 28.458096681702795 22.75763829282101 88-89 21.310187874205013 27.338107814880164 28.30067544119205 23.051028869722774 90-91 21.1980434738932 27.30926518855673 28.557938706961266 22.934752630588804 92-93 21.30313317095312 27.61166836079093 28.40685649991187 22.67834196834408 94-95 21.19883948525438 27.58431807890624 28.15699814492923 23.059844290910153 96-97 21.19187763684188 27.68509363213813 28.314877152041866 22.808151578978123 98-99 21.42754073208154 28.084861547453787 27.543289252575438 22.944308467889236 100-101 21.41448086757653 27.84217840725624 27.671590596563938 23.07175012860329 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 468.0 1 322.5 2 201.0 3 245.0 4 244.5 5 267.0 6 403.0 7 763.0 8 1289.0 9 1501.5 10 1302.0 11 1144.0 12 1190.5 13 1250.0 14 1290.0 15 1305.0 16 1345.0 17 1434.5 18 1480.0 19 1577.5 20 1709.5 21 1888.5 22 2159.5 23 2482.5 24 2836.0 25 3312.0 26 4025.0 27 4767.5 28 5320.5 29 5809.5 30 6604.5 31 7710.0 32 8948.5 33 10314.0 34 11902.5 35 13008.5 36 14211.0 37 15647.0 38 16175.5 39 16209.0 40 16247.5 41 16916.5 42 17597.5 43 18183.0 44 19076.5 45 19631.5 46 19616.5 47 20922.5 48 24181.0 49 23633.5 50 20021.5 51 18012.5 52 16710.5 53 16032.5 54 15660.5 55 14474.5 56 12971.0 57 11638.5 58 10367.5 59 9134.5 60 7866.0 61 6501.0 62 5140.5 63 3846.0 64 2752.0 65 2097.0 66 1507.5 67 1093.5 68 757.0 69 448.0 70 287.5 71 185.0 72 127.5 73 90.0 74 60.5 75 42.0 76 32.0 77 19.5 78 15.5 79 10.0 80 6.0 81 6.5 82 5.0 83 3.0 84 2.5 85 2.0 86 1.0 87 1.0 88 2.5 89 2.0 90 0.0 91 0.0 92 0.5 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04107533398810458 2 0.030852139751065218 3 0.027931227111911115 4 0.05768802462329355 5 0.11008189508812029 6 0.14495028971802237 7 0.08014254053679072 8 0.10716098244896617 9 0.09584244597224403 10-11 0.09958486529116023 12-13 0.09465582521258767 14-15 0.08096404721655281 16-17 0.06672459810067655 18-19 0.07594372861800669 20-21 0.07001062481972492 22-23 0.040253827308342496 24-25 0.00921913051733014 26-27 0.003286026719048367 28-29 0.007211003077911694 30-31 0.006754610478043864 32-33 0.007667395677779522 34-35 0.008397623837568048 36-37 0.007576117157805956 38-39 0.005294154158466813 40-41 0.006937167517990996 42-43 0.002190684479365578 44-45 0.001095342239682789 46-47 0.0011866207596563545 48-49 1.8255703994713148E-4 50-51 0.001734291879497749 52-53 0.005933103798281773 54-55 0.012505157236378508 56-57 0.009949358677118665 58-59 0.005294154158466813 60-61 0.0030121911591276694 62-63 0.010040637197092231 64-65 0.002829634119180538 66-67 0.022637072953444304 68-69 0.04682588074643923 70-71 0.06279962174181324 72-73 0.0029209126391541037 74-75 0.010770865356880757 76-77 0.023184744073285697 78-79 0.02948296195146173 80-81 0.08671459397488746 82-83 0.03194748199074801 84-85 0.015152234315611912 86-87 0.03724163614921482 88-89 0.05111597118519682 90-91 0.0498380719055669 92-93 0.053580491224483086 94-95 0.01569990543545331 96-97 0.022910908513365 98-99 0.05367176974445666 100-101 0.10013253641100162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 547774.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.85192688855589 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49888988850171 51.02715305770631 2 10.25706344395557 12.688382764536385 3 2.9277397847855506 5.4325904135181675 4 1.2804157950721025 3.1678473657500734 5 0.7297276951132595 2.2567532023349863 6 0.4527909973974827 1.6803597400095238 7 0.3089824754741423 1.3377813038010165 8 0.2485862245140296 1.2300429587315118 9 0.17810415077563801 0.9914476421090785 >10 1.0245062228583746 11.604294023524249 >50 0.06665092205283708 2.8255458797410014 >100 0.023298328449356424 2.657362724901893 >500 0.001769493299951061 0.7741520733445509 >1k 0.0014745777499592177 2.326286849991295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4675 0.8534541617528396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3213 0.5865557693501335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2207 0.40290338716331914 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1528 0.2789471570392169 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1130 0.2062894551402586 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 926 0.16904781899104374 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 766 0.1398386925995027 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 722 0.1318061828418289 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 684 0.12486901532383793 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 573 0.10460518388970634 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 573 0.10460518388970634 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.8255703994713148E-4 0.0 16-17 0.0 0.0 0.0 5.476711198413944E-4 0.0 18-19 0.0 0.0 0.0 7.302281597885259E-4 0.0 20-21 0.0 0.0 0.0 9.127851997356574E-4 0.0 22-23 0.0 0.0 0.0 0.0016430133595241835 0.0 24-25 0.0 0.0 0.0 0.003103469679101235 0.0 26-27 0.0 0.0 0.0 0.0040162548788368925 0.0 28-29 0.0 0.0 0.0 0.006663331958070299 0.0 30-31 0.0 0.0 0.0 0.012778992796299203 0.0 32-33 0.0 0.0 0.0 0.017434197314951057 0.0 34-35 0.0 0.0 0.0 0.023276022593259265 0.0 36-37 0.0 0.0 0.0 0.029209126391541038 0.0 38-39 0.0 0.0 0.0 0.042079397707813805 0.0 40-41 0.0 0.0 0.0 0.06572053438096734 0.0 42-43 0.0 0.0 0.0 0.10716098244896619 0.0 44-45 0.0 0.0 0.0 0.14686713863746728 0.0 46-47 0.0 0.0 0.0 0.2459043328087861 0.0 48-49 0.0 0.0 0.0 0.3372741313023254 0.0 50-51 0.0 0.0 0.0 0.3979743470847466 0.0 52-53 0.0 0.0 0.0 0.4750134179424361 0.0 54-55 0.0 0.0 0.0 0.5550646799592532 0.0 56-57 0.0 0.0 0.0 0.7235648278304556 0.0 58-59 0.0 0.0 0.0 0.9458280239660882 0.0 60-61 0.0 0.0 0.0 1.1817829980977557 0.0 62-63 0.0 0.0 0.0 1.4217542271082602 0.0 64-65 0.0 0.0 0.0 1.7007926626674505 0.0 66-67 0.0 0.0 0.0 2.0588782965237487 0.0 68-69 0.0 0.0 0.0 2.4699967504846887 0.0 70-71 0.0 0.0 0.0 2.7998773216691557 0.0 72-73 0.0 0.0 0.0 3.0722524252702756 0.0 74-75 0.0 0.0 0.0 3.36854250110447 0.0 76-77 0.0 0.0 0.0 3.720877588202434 0.0 78-79 0.0 0.0 0.0 4.070291762661244 0.0 80-81 0.0 0.0 0.0 4.493915373858562 0.0 82-83 0.0 0.0 0.0 4.881757805226243 0.0 84-85 0.0 0.0 0.0 5.449601477981796 0.0 86-87 0.0 0.0 0.0 6.0855389266376285 0.0 88-89 0.0 0.0 0.0 6.978425409019048 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACG 35 2.7683133E-4 54.35832 4 TATACGT 60 0.0039234017 31.709019 5 GTATCAA 5115 0.0 28.07734 1 GGTATCA 3630 0.0 25.807993 1 GAGTACT 2865 0.0 24.549007 12-13 CTAGACA 155 1.3920799E-6 24.54892 4 GTACATG 3310 0.0 24.28026 1 TCAACGC 6170 0.0 23.28069 4 TATCAAC 6180 0.0 23.24302 2 ATCAACG 6190 0.0 23.128632 3 CAACGCA 6185 0.0 23.070425 5 TACATGG 3460 0.0 22.819496 2 AACGCAG 6320 0.0 22.573494 6 AGTACTT 3065 0.0 22.481968 12-13 TAGACAG 170 3.107718E-6 22.382837 5 ACATGGG 3440 0.0 22.122572 3 GTACTTT 3115 0.0 22.041801 14-15 ATACAGT 240 5.83168E-9 21.795961 6 AGAGTAC 5010 0.0 21.484602 10-11 ACGCAGA 6885 0.0 20.575392 7 >>END_MODULE