FastQCFastQC Report
Thu 26 May 2016
SRR937120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547774
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51400.9383431853282559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39690.7245688915501649No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26350.48103780026069143No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20480.37387681781172527No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10150.18529539554633845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.16521412115215398No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.15699905435453307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8580.1566339402746388No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.13710033700029575No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7110.1297980554024105No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5880.10734353948891331No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG250.005014607757.015128
TACATCG700.00839189227.1401465
CTTAGAC1109.474187E-525.9065023
GTACATG30550.024.4195081
GTATCAA70300.024.3331
CGGTTAC701.780759E-523.7476328-29
AGTACTT39400.023.15229212-13
ACTTGAC1652.4204364E-623.0364158
GAGTACT38450.022.9829412-13
GGACGCT1903.280711E-722.4998056
TACATGG33100.022.3865132
ACATGGG32600.021.8536433
GTATAGG1553.890902E-521.4592651
CTGGACG2005.3806434E-721.3728644
GTACTTT41850.021.22539714-15
CATGGGA22150.021.0137024
GGTATCA53050.020.9594021
TGGACGC2056.822356E-720.8515745
AGAGTAC62400.020.59640910-11
CAACGCA83600.020.3956895
Produced by FastQC (version 0.11.5)