##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937120_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547774 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.947509374304 31.0 31.0 34.0 30.0 34.0 2 32.06714265372216 33.0 31.0 34.0 30.0 34.0 3 32.16855674055358 33.0 31.0 34.0 30.0 34.0 4 35.89935630387715 37.0 35.0 37.0 35.0 37.0 5 35.71003735117037 37.0 35.0 37.0 33.0 37.0 6 35.81201736482564 37.0 35.0 37.0 35.0 37.0 7 35.76110403195479 37.0 35.0 37.0 33.0 37.0 8 35.72904519016967 37.0 35.0 37.0 33.0 37.0 9 37.35239167978035 39.0 37.0 39.0 34.0 39.0 10-11 37.27236780131952 39.0 37.0 39.0 34.0 39.0 12-13 37.156781811476996 39.0 37.0 39.0 33.0 39.0 14-15 38.24876408883956 40.0 38.0 41.0 33.0 41.0 16-17 38.25719913687031 40.0 37.5 41.0 33.0 41.0 18-19 38.40698627536174 40.0 38.0 41.0 33.5 41.0 20-21 38.43812411688032 40.0 38.0 41.0 34.0 41.0 22-23 38.32515599499064 40.0 38.0 41.0 34.0 41.0 24-25 38.201071062153375 40.0 37.5 41.0 33.0 41.0 26-27 37.96869694436027 40.0 37.0 41.0 33.0 41.0 28-29 37.7848720457707 40.0 37.0 41.0 33.0 41.0 30-31 37.70043667643955 40.0 37.0 41.0 33.0 41.0 32-33 37.50971386009559 40.0 36.0 41.0 33.0 41.0 34-35 37.30993438899985 39.0 36.0 41.0 32.0 41.0 36-37 37.105433080065865 39.0 35.5 41.0 31.5 41.0 38-39 36.89729706046654 39.0 35.0 41.0 31.0 41.0 40-41 36.45348172786587 39.0 35.0 41.0 30.5 41.0 42-43 36.22157769445063 39.0 35.0 40.5 30.0 41.0 44-45 35.90223705396751 39.0 35.0 40.0 29.5 41.0 46-47 35.35929051031995 38.5 35.0 40.0 27.0 41.0 48-49 34.850503127202096 38.0 34.5 40.0 24.5 41.0 50-51 34.50841861789716 38.0 34.0 40.0 23.5 41.0 52-53 34.241883514003945 38.0 34.0 40.0 23.0 41.0 54-55 33.88039045299704 38.0 33.0 40.0 22.0 41.0 56-57 33.61940417033302 38.0 33.0 40.0 20.5 41.0 58-59 33.26931362204121 37.0 33.0 40.0 19.5 41.0 60-61 32.83832200871163 37.0 32.0 39.5 18.0 41.0 62-63 32.45904241530266 36.0 31.5 39.0 14.5 40.0 64-65 32.1277433759178 36.0 31.0 39.0 13.0 40.0 66-67 31.782382880531024 35.0 31.0 39.0 8.0 40.0 68-69 31.287236159438017 35.0 30.5 38.0 7.0 40.0 70-71 30.827896176160245 35.0 30.0 37.5 4.0 39.5 72-73 30.393554641147624 34.5 29.5 37.0 2.0 39.0 74-75 29.901583499764502 34.0 29.0 36.5 2.0 39.0 76-77 28.46606082070343 32.5 27.0 35.0 2.0 37.0 78-79 29.13141642356154 34.0 29.0 35.0 2.0 37.0 80-81 28.98145676866737 34.0 29.0 35.0 2.0 37.0 82-83 28.631656486069073 34.0 29.0 35.0 2.0 36.5 84-85 28.191774344894064 33.5 27.5 35.0 2.0 36.0 86-87 27.793051513945535 33.0 27.0 35.0 2.0 36.0 88-89 27.385236429622438 33.0 26.0 35.0 2.0 35.0 90-91 26.903056735076873 33.0 25.5 35.0 2.0 35.0 92-93 26.385518480249154 32.0 25.0 34.0 2.0 35.0 94-95 25.7990320825742 32.0 23.5 34.0 2.0 35.0 96-97 25.019327313819204 32.0 19.5 34.0 2.0 35.0 98-99 24.17958683690719 31.0 9.5 34.0 2.0 35.0 100-101 21.301926524442564 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32742757209105733 1101 2 0.49290221311560245 1101 3 0.36686258741647393 1101 4 0.27515431344831853 1101 5 0.09361186469309501 1101 6 0.26677931848455216 1101 7 0.2837222489760194 1101 8 0.3453810820203316 1101 9 0.25802819355238427 1101 10-11 0.3941266788391786 1101 12-13 0.4480129655502054 1101 14-15 0.635454769416981 1101 16-17 0.5942139379172389 1101 18-19 0.45409861791224415 1101 20-21 0.3560955817417977 1101 22-23 0.38777396539549613 1101 24-25 0.4952172586643968 1101 26-27 0.5352726971261603 1101 28-29 0.5488036387647668 1101 30-31 0.7059708614436815 1101 32-33 0.75005181075894 1101 34-35 0.6607492548905256 1101 36-37 0.7360716588070559 1101 38-39 0.693079548541057 1101 40-41 0.9803104250658734 1101 42-43 1.0362530319494425 1101 44-45 1.004019880555063 1101 46-47 1.1183638257576476 1101 48-49 1.1981584887926289 1101 50-51 1.1989832515459256 1101 52-53 1.2269831957541442 1101 54-55 1.1511447210925425 1101 56-57 1.2680822013927298 1101 58-59 1.314879829390776 1101 60-61 1.3756950859614037 1101 62-63 1.5138844959761038 1101 64-65 1.4780801296122057 1101 66-67 1.6389782588350101 1101 68-69 1.3260486889247716 1101 70-71 1.1582016743356078 1101 72-73 1.4320082383161186 1101 74-75 1.2271659477618861 1101 76-77 1.3489155468401108 1101 78-79 1.5272030292680405 1101 80-81 1.5189351417361934 1101 82-83 1.747029141506161 1101 84-85 1.677768532934909 1101 86-87 1.3755388056593674 1101 88-89 1.4489628715481366 1101 90-91 1.707132941526229 1101 92-93 1.5940745088081165 1101 94-95 1.4925102847427816 1101 96-97 1.3477401092493189 1101 98-99 1.6754733046641022 1101 100-101 1.8024353868419034 1102 1 0.07173888799386319 1102 2 0.11583611901084012 1102 3 0.22901600836237535 1102 4 0.1601189185854892 1102 5 0.07266032199556349 1102 6 0.19786177827072038 1102 7 0.1972034476764648 1102 8 0.36499564806216966 1102 9 0.32366339858334214 1102 10-11 0.3495015549676097 1102 12-13 0.4356205822403538 1102 14-15 0.42980026760019996 1102 16-17 0.38554575064434005 1102 18-19 0.48328317713853863 1102 20-21 0.3677734822180554 1102 22-23 0.17160135637806206 1102 24-25 0.17830257010991346 1102 26-27 0.40592882965723476 1102 28-29 0.21144125641662015 1102 30-31 0.3524367063418481 1102 32-33 0.3067447430759316 1102 34-35 0.33871872290134775 1102 36-37 0.36847363824333 1102 38-39 0.46920076915160536 1102 40-41 0.4146407241555963 1102 42-43 0.6652650072462123 1102 44-45 0.5367977605428536 1102 46-47 0.6594228074392916 1102 48-49 0.8162159317489923 1102 50-51 0.9098011592976718 1102 52-53 1.0295120573064978 1102 54-55 1.2781068048945414 1102 56-57 1.0791142971951047 1102 58-59 0.7789241471877233 1102 60-61 1.0133221457835262 1102 62-63 0.9330094295976714 1102 64-65 1.09071335687943 1102 66-67 1.057998136824125 1102 68-69 1.0536880418238894 1102 70-71 0.8296090853048774 1102 72-73 0.6988244568151067 1102 74-75 0.8122547380645706 1102 76-77 0.8313843228426876 1102 78-79 0.6429983788053804 1102 80-81 0.9918490818346228 1102 82-83 1.2044544317942147 1102 84-85 1.0152594908332127 1102 86-87 0.9120249926730288 1102 88-89 1.0797984244648156 1102 90-91 1.0711402546526436 1102 92-93 1.0222279548419273 1102 94-95 1.006409510780692 1102 96-97 1.212262247241199 1102 98-99 1.3892082048769332 1102 100-101 1.2773349306600288 1103 1 0.11254208104393015 1103 2 0.21262960828617494 1103 3 0.16505745509597247 1103 4 0.16565669464245047 1103 5 0.033935212873466014 1103 6 0.04827830251654319 1103 7 0.10510455266054208 1103 8 0.12252076202405249 1103 9 0.30398285483317267 1103 10-11 0.236566616841273 1103 12-13 0.30941101337471366 1103 14-15 0.39564686749795897 1103 16-17 0.300077743154759 1103 18-19 0.24496824004065587 1103 20-21 0.31514797290600427 1103 22-23 0.301855551112979 1103 24-25 0.3305812027488457 1103 26-27 0.37337596253664174 1103 28-29 0.24981850578367215 1103 30-31 0.5994764954480871 1103 32-33 0.6401966764199543 1103 34-35 0.5343202631638491 1103 36-37 0.4009747045091032 1103 38-39 0.45319732987733374 1103 40-41 0.648413066582691 1103 42-43 0.6910123007460669 1103 44-45 0.7405902637114039 1103 46-47 0.7316494577313435 1103 48-49 0.670300107759978 1103 50-51 0.7333130001412442 1103 52-53 0.7694611790362345 1103 54-55 1.1203913511492942 1103 56-57 0.8213052238027458 1103 58-59 0.665988830952962 1103 60-61 0.5288389595091658 1103 62-63 0.9528389480690187 1103 64-65 0.8032299558394804 1103 66-67 0.9444714590057828 1103 68-69 0.8799683925875144 1103 70-71 0.8955198123922621 1103 72-73 0.992526229084941 1103 74-75 1.0544467989974997 1103 76-77 1.1135522755821583 1103 78-79 1.036563880797253 1103 80-81 1.0830885363905267 1103 82-83 1.3986875976507172 1103 84-85 0.9723698954697291 1103 86-87 0.9400836630018006 1103 88-89 1.2424629394424471 1103 90-91 0.8900350134648356 1103 92-93 0.947439619940706 1103 94-95 0.8358398828696352 1103 96-97 0.801441388965312 1103 98-99 0.7673018880462692 1103 100-101 1.2551399658543652 1104 1 -0.06738688673934945 1104 2 0.1017627698333392 1104 3 0.02727858661236482 1104 4 0.10041203569252843 1104 5 -0.06679942294439911 1104 6 0.01769650185212157 1104 7 -0.005594135311248749 1104 8 0.07176203978592355 1104 9 0.09412878218307696 1104 10-11 0.12312412203348799 1104 12-13 0.11377815404394909 1104 14-15 0.2762691151699812 1104 16-17 0.16794668576091 1104 18-19 0.1956649283850993 1104 20-21 0.032762531101973025 1104 22-23 0.19760054863616006 1104 24-25 0.1924438783411162 1104 26-27 0.019010753325623853 1104 28-29 0.0232238029782863 1104 30-31 0.15131285776238457 1104 32-33 0.3046069454152729 1104 34-35 0.3248803178809183 1104 36-37 0.31502481335245847 1104 38-39 0.15269992257596954 1104 40-41 0.4569112555565624 1104 42-43 0.15791960438494357 1104 44-45 0.42614488762240654 1104 46-47 0.2647535156114671 1104 48-49 0.4941715680787411 1104 50-51 0.723137055658178 1104 52-53 0.6525664866834475 1104 54-55 0.7795378283967196 1104 56-57 0.7915096402524071 1104 58-59 0.22416662509132124 1104 60-61 0.5518704627290205 1104 62-63 0.6022701904284027 1104 64-65 0.5809350198957226 1104 66-67 0.36726425671166396 1104 68-69 0.43994982786548675 1104 70-71 0.4782782528647189 1104 72-73 0.5857134078379893 1104 74-75 0.39807537276329796 1104 76-77 0.5789416802840073 1104 78-79 0.9772345967092875 1104 80-81 0.717259554518968 1104 82-83 0.8132665852030385 1104 84-85 0.8481015772926632 1104 86-87 0.975135653312698 1104 88-89 0.9367795667982577 1104 90-91 0.9488547334261206 1104 92-93 0.6423970369675338 1104 94-95 0.6519525618703668 1104 96-97 0.9568857152499568 1104 98-99 0.6769817886102558 1104 100-101 1.033975501050076 1105 1 0.1400778740309505 1105 2 0.14990650618434032 1105 3 0.2939138192243753 1105 4 0.16103840059226826 1105 5 -0.22523533056136813 1105 6 -0.03080293564374159 1105 7 0.1618997433202125 1105 8 0.2250526889818758 1105 9 0.24308484068350822 1105 10-11 0.07629151664668399 1105 12-13 0.22185753330408886 1105 14-15 0.1168240089559589 1105 16-17 -0.040229477299106975 1105 18-19 0.15329099548853264 1105 20-21 -0.031350532229375005 1105 22-23 0.01582899149236283 1105 24-25 -0.05721696475332294 1105 26-27 0.12126656035362515 1105 28-29 0.16211682792699378 1105 30-31 0.14246996185873684 1105 32-33 0.20109791226062157 1105 34-35 0.20377235017527795 1105 36-37 0.3033445804095649 1105 38-39 0.38009635563797417 1105 40-41 0.27765442278573715 1105 42-43 0.5229930608074156 1105 44-45 0.48853321400715544 1105 46-47 0.3868954982730983 1105 48-49 0.24669042851153478 1105 50-51 0.24944239613861185 1105 52-53 0.22743744161694224 1105 54-55 0.49646175798443437 1105 56-57 0.40078434080167824 1105 58-59 0.21142756083349212 1105 60-61 0.24348170619405352 1105 62-63 0.4958783235764379 1105 64-65 0.5260743223905919 1105 66-67 0.3479157688551382 1105 68-69 0.5344339784842376 1105 70-71 0.29972041420414186 1105 72-73 0.2507173784554233 1105 74-75 0.10068853536008149 1105 76-77 -0.011323689314298946 1105 78-79 0.1371015810253482 1105 80-81 -0.05825993459492551 1105 82-83 0.35299757587208447 1105 84-85 0.03950188170666635 1105 86-87 -0.022845949493206064 1105 88-89 0.21813917238359437 1105 90-91 0.5318530459137101 1105 92-93 0.5419197356638428 1105 94-95 0.9178391676542326 1105 96-97 0.9903009425678064 1105 98-99 1.0705113366216352 1105 100-101 1.2143283896742574 1106 1 -0.10293718089920034 1106 2 0.028653284971809967 1106 3 -0.12323320369709734 1106 4 0.12670705649895808 1106 5 -0.10749153563393321 1106 6 0.05079546392224188 1106 7 0.04044307710564965 1106 8 -0.009705889615716501 1106 9 0.20871958908985278 1106 10-11 -0.03545912759582137 1106 12-13 0.09432294765374394 1106 14-15 0.22561622379389235 1106 16-17 -0.261425055761066 1106 18-19 -0.1137051390564281 1106 20-21 -0.19686151825948173 1106 22-23 0.2897168254812428 1106 24-25 0.0599164951680109 1106 26-27 0.26126215237857764 1106 28-29 0.2590838020216637 1106 30-31 0.23673281280137104 1106 32-33 0.2865448131150927 1106 34-35 0.1427642801901996 1106 36-37 0.2582136974582525 1106 38-39 0.34790966094110587 1106 40-41 0.4690961697001512 1106 42-43 0.6710671147881584 1106 44-45 0.3413407550352261 1106 46-47 0.19707283449987045 1106 48-49 -0.21403620922073685 1106 50-51 -0.5938201835440111 1106 52-53 -0.3724371006318066 1106 54-55 -0.10189542362413562 1106 56-57 -0.07086898891485305 1106 58-59 -0.15601106659088515 1106 60-61 0.08471831273141817 1106 62-63 0.30970071608966165 1106 64-65 0.2558824059386744 1106 66-67 -0.04329201630694257 1106 68-69 0.2222476228623762 1106 70-71 0.0382342484642777 1106 72-73 -0.15698640562161614 1106 74-75 -0.4303792118255956 1106 76-77 -0.24826930168375583 1106 78-79 -0.15655432566120808 1106 80-81 -0.350678217519107 1106 82-83 0.0900279569923903 1106 84-85 0.37230399753468646 1106 86-87 0.22773047801255686 1106 88-89 0.5219842829220447 1106 90-91 0.5327261640474426 1106 92-93 0.7487131033567813 1106 94-95 0.5783827048846675 1106 96-97 0.4619797029095949 1106 98-99 0.20408993420742405 1106 100-101 0.7661644808176185 1107 1 0.10588818668739464 1107 2 0.1485753270098975 1107 3 0.03785524734069767 1107 4 0.08859153398324793 1107 5 -0.027987571948528966 1107 6 -0.12132880830564119 1107 7 0.005764941518002331 1107 8 -0.0884286289992886 1107 9 -0.10543948462482433 1107 10-11 0.03952195855266183 1107 12-13 0.15940304370322167 1107 14-15 0.13892499653162815 1107 16-17 -0.1365977474488318 1107 18-19 -0.03452188850545923 1107 20-21 -0.1373527849941354 1107 22-23 -0.11052494288163928 1107 24-25 0.006599992421399747 1107 26-27 -0.1381284926659916 1107 28-29 -0.18168294907204796 1107 30-31 -0.24176296036399236 1107 32-33 -0.33162975351100954 1107 34-35 0.012517742247361241 1107 36-37 -0.24130112113324031 1107 38-39 -0.031946264850809314 1107 40-41 -0.15844897684107906 1107 42-43 0.04569706117149508 1107 44-45 -0.170110348546892 1107 46-47 -0.28250842927434405 1107 48-49 -0.34871927885659915 1107 50-51 -0.3333353310720497 1107 52-53 -0.48072712398629847 1107 54-55 -0.5574026437813657 1107 56-57 -0.7037758011907655 1107 58-59 -0.6875848635054638 1107 60-61 -0.908986664078558 1107 62-63 -0.6613117506055133 1107 64-65 -0.958201834711307 1107 66-67 -1.0759965326057461 1107 68-69 -1.26878474449396 1107 70-71 -1.133478853078433 1107 72-73 -1.0293137961679797 1107 74-75 -0.8997182138321058 1107 76-77 -1.0653559106768782 1107 78-79 -0.9355500823797414 1107 80-81 -0.6761773787309089 1107 82-83 -0.39239449621638656 1107 84-85 -0.3345765327110293 1107 86-87 -0.11565871971120245 1107 88-89 -0.5137135559648343 1107 90-91 -0.5144680071969283 1107 92-93 -0.41797138070624484 1107 94-95 -0.10672222587418645 1107 96-97 -0.1735791602856942 1107 98-99 0.4446864606374703 1107 100-101 0.40271015365732765 1108 1 -0.38179398031715905 1108 2 -0.22688241236789253 1108 3 -0.18054348744112758 1108 4 -0.18363204450379556 1108 5 -0.2789083578273335 1108 6 0.04615338629594845 1108 7 -0.1586285073690945 1108 8 -0.38996875615795545 1108 9 -0.07463694145138078 1108 10-11 -0.12602313607334992 1108 12-13 -0.14352224261607915 1108 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-0.8060723599515462 2316 90-91 -0.8435562316339862 2316 92-93 -0.6806144224190547 2316 94-95 -1.1019234071218804 2316 96-97 -0.8865728849073271 2316 98-99 -0.8869857431277524 2316 100-101 -1.2082163875017713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 11.0 5 23.0 6 67.0 7 95.0 8 261.0 9 486.0 10 852.0 11 1413.0 12 2028.0 13 2813.0 14 3484.0 15 4944.0 16 6575.0 17 9180.0 18 7719.0 19 4672.0 20 4028.0 21 3983.0 22 4162.0 23 4582.0 24 5192.0 25 5998.0 26 7421.0 27 8574.0 28 10539.0 29 12706.0 30 15806.0 31 19377.0 32 23769.0 33 29933.0 34 39903.0 35 54728.0 36 79551.0 37 107668.0 38 62335.0 39 2895.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44352843435312 20.260925930997963 15.605342682114065 20.690202952534854 2 15.50742682713092 22.159256879126016 39.48578167903254 22.847534614710522 3 18.920897456961864 27.120506782043556 31.170564287931068 22.788031473063512 4 12.565035945481165 18.40631355266953 38.13726098719545 30.891389514653856 5 12.734452302625574 38.93980276249348 33.66510517264671 14.66063976223424 6 29.164034802674095 38.19330599845922 17.606348603621203 15.036310595245483 7 26.450200723297595 35.12885906285314 21.277582338540515 17.14335787530875 8 26.49158229488804 33.76428965230186 20.049144355153768 19.694983697656333 9 26.881622280802965 16.905756409601075 21.215213629028135 34.997407680567825 10-11 24.320522697316775 27.572137414335106 27.155816084735672 20.95152380361244 12-13 26.435094407139232 25.62813336400516 27.919665385022657 20.017106843832956 14-15 22.961890538907287 25.946072757413212 28.11389752034732 22.97813918333218 16-17 20.509644488420406 29.725397700511525 28.10922022585957 21.6557375852085 18-19 20.473755504114852 27.4583220756081 31.968501493322037 20.099420926955013 20-21 21.86788591541325 25.876236150640143 32.083049606677264 20.17282832726934 22-23 21.37139101622046 26.214982702436263 32.208793917099484 20.204832364243792 24-25 21.530895209859594 26.34741525199399 32.13213185802096 19.989557680125454 26-27 21.085833000166126 26.474013870709218 32.45742305663112 19.98273007249353 28-29 20.410370719257795 26.965914712846377 32.36377112417005 20.259943443725778 30-31 20.76394644506676 26.328467579695275 32.29415780960761 20.613428165630353 32-33 20.682982397850207 26.695498508508987 32.17741258256142 20.444106511079386 34-35 21.278666019197697 26.430608243545695 31.876193466648655 20.414532270607953 36-37 21.371593084254272 26.09971986136378 32.13780739715699 20.390879657224957 38-39 20.99315421770117 26.698094519083565 31.99643241535887 20.312318847856393 40-41 20.811506257627496 26.694251161296272 31.902809839504247 20.59143274157199 42-43 20.79945378933648 26.953269048914336 31.15901813521635 21.08825902653284 44-45 21.189434309467607 26.960437991649826 30.76894991173351 21.081177787149056 46-47 21.57952002103057 27.24526903430977 30.3332213650155 20.84198957964416 48-49 21.81301047512295 27.54448002278312 29.616593704703035 21.025915797390894 50-51 21.260093361885932 27.8369580893857 29.63902139801848 21.26392715070989 52-53 21.64080741475027 28.228664063398522 29.042596111893065 21.08793240995814 54-55 21.253108033605102 27.91306268643638 29.199359589903867 21.634469690054658 56-57 21.65657885969292 27.93910256702816 28.9876198830356 21.416698690243322 58-59 21.992849234218156 27.510184410162232 29.054618377851433 21.442347977768183 60-61 21.638575112389038 27.54916592501312 29.679468747860614 21.132790214737227 62-63 21.26670853308116 27.653740411191475 29.819962247204142 21.259588808523223 64-65 21.167488781869896 27.820780102743104 29.509341443734094 21.502389671652907 66-67 21.323666329544665 27.773315272356847 29.08608294661667 21.816935451481815 68-69 21.22273165824926 27.74121055509257 29.22366635114696 21.81239143551121 70-71 21.464326985743938 27.72839546741589 29.029130999129194 21.778146547710982 72-73 21.396701894411695 27.53204361692611 29.318289278874854 21.752965209787337 74-75 21.104659282919005 28.287336291579223 28.608020856820232 21.999983568681543 76-77 21.262437850680985 28.077572208221095 28.621775241043974 22.038214700053945 78-79 21.24957327058205 28.01672596760325 28.47467281035323 22.25902795146147 80-81 21.253425395301115 28.18463633757921 28.284226111690252 22.277712155429423 82-83 21.17364309668421 27.94579141359651 28.573514941408213 22.307050548311068 84-85 20.85262458374715 28.05133419991821 28.197752964888707 22.89828825144593 86-87 20.961409792640502 27.95916523129753 28.14720087044071 22.932224105621263 88-89 21.09696206229233 27.596122247781796 27.908222879468358 23.398692810457515 90-91 20.963030579644 27.557371063441348 28.365495207667728 23.11410314924692 92-93 20.92879545213902 27.753051440922714 28.214555637909065 23.1035974690292 94-95 20.550263429808645 27.72338592193131 28.12227305421579 23.60407759404426 96-97 20.690511311358094 27.982284470797858 28.064709877174277 23.26249434066977 98-99 20.639239813056815 28.209891193223307 27.325836132612825 23.825032861107054 100-101 20.189647350496163 27.881154570440902 27.504251456175087 24.42494662288785 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 685.0 1 482.5 2 303.0 3 316.5 4 285.5 5 279.5 6 445.5 7 844.0 8 1341.5 9 1658.0 10 1572.0 11 1311.5 12 1247.5 13 1311.5 14 1380.0 15 1466.5 16 1553.0 17 1644.5 18 1695.5 19 1777.5 20 1874.0 21 2049.5 22 2266.5 23 2550.5 24 2878.0 25 3414.0 26 4243.5 27 4875.0 28 5348.0 29 5866.5 30 6621.0 31 7629.5 32 8808.5 33 10151.5 34 11726.5 35 12943.5 36 14191.5 37 15554.5 38 16108.0 39 15923.5 40 15928.0 41 16683.0 42 17437.5 43 18320.0 44 19387.5 45 19963.5 46 19567.0 47 20632.5 48 24167.0 49 23509.0 50 19857.5 51 17944.0 52 16709.5 53 16028.5 54 15219.5 55 14133.0 56 12693.0 57 11314.0 58 10280.0 59 9158.0 60 7911.0 61 6401.5 62 5085.0 63 3912.0 64 2736.5 65 2017.5 66 1444.0 67 1080.0 68 745.0 69 408.0 70 269.0 71 180.5 72 114.0 73 93.5 74 65.0 75 37.5 76 26.0 77 12.5 78 9.0 79 5.0 80 3.0 81 2.5 82 1.0 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01989871735423733 2 0.0054767111984139446 3 7.302281597885259E-4 4 0.0 5 0.0014604563195770518 6 0.0 7 9.127851997356574E-4 8 0.0 9 3.6511407989426296E-4 10-11 0.0 12-13 0.007302281597885259 14-15 0.006572053438096734 16-17 0.0 18-19 3.6511407989426296E-4 20-21 9.127851997356574E-4 22-23 0.0016430133595241835 24-25 5.476711198413945E-4 26-27 1.8255703994713148E-4 28-29 1.8255703994713148E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.001734291879497749 38-39 0.01177492907658998 40-41 4.563925998678287E-4 42-43 0.0 44-45 1.8255703994713148E-4 46-47 0.0 48-49 0.0 50-51 0.002373241519312709 52-53 1.8255703994713148E-4 54-55 0.0 56-57 9.127851997356574E-5 58-59 9.127851997356574E-5 60-61 0.002099405959392012 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.127851997356574E-5 70-71 0.0012778992796299202 72-73 9.127851997356574E-4 74-75 0.007211003077911694 76-77 9.127851997356574E-5 78-79 5.476711198413945E-4 80-81 0.00492904007857255 82-83 1.8255703994713148E-4 84-85 0.0054767111984139446 86-87 0.0010040637197092231 88-89 0.006206939358202471 90-91 0.004381368958731156 92-93 0.0 94-95 0.0 96-97 0.001095342239682789 98-99 0.0025557985592598404 100-101 0.0037424193189161954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 547774.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.64704559058617 #Duplication Level Percentage of deduplicated Percentage of total 1 81.58423300673 48.66258465625429 2 10.596792557168154 12.641347375427861 3 3.1847506834483625 5.698829076308849 4 1.3945538418193382 3.327240663261006 5 0.7770966950993514 2.3175761000442425 6 0.48803019352830795 1.7465735521779342 7 0.3319144861664124 1.3858402941980787 8 0.23945287055766257 1.1426125029559702 9 0.18452291742734195 0.9905622181466968 >10 1.111210261449923 12.125264218475925 >50 0.07183297843122965 2.874177638506891 >100 0.031618787430269316 3.334499969668473 >500 0.0024558281499238014 1.041676477263402 >1k 0.0012279140749619007 1.7700626657945695 >5k 3.069785187404752E-4 0.9411525915158878 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5140 0.9383431853282559 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3969 0.7245688915501649 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2635 0.48103780026069143 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2048 0.37387681781172527 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1015 0.18529539554633845 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 905 0.16521412115215398 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 860 0.15699905435453307 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 858 0.1566339402746388 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 751 0.13710033700029575 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 711 0.1297980554024105 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 588 0.10734353948891331 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.8255703994713148E-4 0.0 16-17 0.0 0.0 0.0 5.476711198413944E-4 0.0 18-19 0.0 0.0 0.0 7.302281597885259E-4 0.0 20-21 0.0 0.0 0.0 9.127851997356574E-4 0.0 22-23 0.0 0.0 0.0 0.0016430133595241835 0.0 24-25 0.0 0.0 0.0 0.0031947481990748008 0.0 26-27 0.0 0.0 0.0 0.004198811918784024 0.0 28-29 0.0 0.0 0.0 0.006937167517990997 0.0 30-31 0.0 0.0 0.0 0.013144106876193468 0.0 32-33 0.0 0.0 0.0 0.017890589914818886 0.0 34-35 0.0 0.0 0.0 0.024188807792994923 0.0 36-37 0.0 0.0 0.0 0.030395747151197392 0.0 38-39 0.0 0.0 0.0 0.04308346142752303 0.0 40-41 0.0 0.0 0.0 0.06645076254075587 0.0 42-43 0.0 0.0 0.0 0.10853016024856967 0.0 44-45 0.0 0.0 0.0 0.1481450379170972 0.0 46-47 0.0 0.0 0.0 0.24709095356844246 0.0 48-49 0.0 0.0 0.0 0.3382781950220346 0.0 50-51 0.0 0.0 0.0 0.39989119600419154 0.0 52-53 0.0 0.0 0.0 0.4774779379817224 0.0 54-55 0.0 0.0 0.0 0.5575291999985396 0.0 56-57 0.0 0.0 0.0 0.727489804189319 0.0 58-59 0.0 0.0 0.0 0.9508483425646344 0.0 60-61 0.0 0.0 0.0 1.186346924096434 0.0 62-63 0.0 0.0 0.0 1.4267745457068062 0.0 64-65 0.0 0.0 0.0 1.7066344879457587 0.0 66-67 0.0 0.0 0.0 2.0647201218020568 0.0 68-69 0.0 0.0 0.0 2.4770251965226535 0.0 70-71 0.0 0.0 0.0 2.8076359958669084 0.0 72-73 0.0 0.0 0.0 3.0806500491078435 0.0 74-75 0.0 0.0 0.0 3.3763924538221968 0.0 76-77 0.0 0.0 0.0 3.7302792757597114 0.0 78-79 0.0 0.0 0.0 4.082249248777781 0.0 80-81 0.0 0.0 0.0 4.507972265934491 0.0 82-83 0.0 0.0 0.0 4.897366432141722 0.0 84-85 0.0 0.0 0.0 5.467309510856667 0.0 86-87 0.0 0.0 0.0 6.101238832073081 0.0 88-89 0.0 0.0 0.0 6.9917520729351885 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 25 0.0050146077 57.01512 8 TACATCG 70 0.008391892 27.140146 5 CTTAGAC 110 9.474187E-5 25.906502 3 GTACATG 3055 0.0 24.419508 1 GTATCAA 7030 0.0 24.333 1 CGGTTAC 70 1.780759E-5 23.74763 28-29 AGTACTT 3940 0.0 23.152292 12-13 ACTTGAC 165 2.4204364E-6 23.036415 8 GAGTACT 3845 0.0 22.98294 12-13 GGACGCT 190 3.280711E-7 22.499805 6 TACATGG 3310 0.0 22.386513 2 ACATGGG 3260 0.0 21.853643 3 GTATAGG 155 3.890902E-5 21.459265 1 CTGGACG 200 5.3806434E-7 21.372864 4 GTACTTT 4185 0.0 21.225397 14-15 CATGGGA 2215 0.0 21.013702 4 GGTATCA 5305 0.0 20.959402 1 TGGACGC 205 6.822356E-7 20.851574 5 AGAGTAC 6240 0.0 20.596409 10-11 CAACGCA 8360 0.0 20.395689 5 >>END_MODULE