##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937119_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 216016 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861732464261905 33.0 31.0 34.0 30.0 34.0 2 31.96771072513147 33.0 31.0 34.0 30.0 34.0 3 31.926084178949708 33.0 31.0 34.0 30.0 34.0 4 35.53497889045256 37.0 35.0 37.0 33.0 37.0 5 35.567934782608695 37.0 35.0 37.0 33.0 37.0 6 35.65709021553959 37.0 35.0 37.0 33.0 37.0 7 35.61130656988371 37.0 35.0 37.0 33.0 37.0 8 35.57103640471076 37.0 35.0 37.0 33.0 37.0 9 37.21548866750611 39.0 37.0 39.0 34.0 39.0 10-11 37.11023720465151 39.0 37.0 39.0 33.0 39.0 12-13 37.05583845641063 39.0 37.0 39.0 33.0 39.0 14-15 38.185044163395304 40.0 38.0 41.0 33.0 41.0 16-17 38.10655229242278 40.0 38.0 41.0 33.0 41.0 18-19 38.0853709910377 40.0 38.0 41.0 33.5 41.0 20-21 38.05276461002889 40.0 38.0 41.0 33.0 41.0 22-23 37.92386212132435 40.0 37.5 41.0 33.0 41.0 24-25 37.77453521961336 40.0 37.0 41.0 33.0 41.0 26-27 37.69170802162803 40.0 37.0 41.0 33.0 41.0 28-29 37.71126675801793 40.0 37.0 41.0 33.0 41.0 30-31 37.55069300422191 40.0 37.0 41.0 33.0 41.0 32-33 37.287606010665876 39.5 36.0 41.0 33.0 41.0 34-35 37.105638008295685 39.0 35.0 41.0 32.0 41.0 36-37 36.94766822827938 39.0 35.0 41.0 32.0 41.0 38-39 36.759719187467596 39.0 35.0 41.0 31.0 41.0 40-41 36.59686088067551 39.0 35.0 41.0 31.0 41.0 42-43 36.383360491815424 39.0 35.0 41.0 30.0 41.0 44-45 36.07874185245538 39.0 35.0 41.0 30.0 41.0 46-47 35.68804394118954 39.0 35.0 41.0 29.0 41.0 48-49 35.310782534627066 39.0 35.0 40.0 27.0 41.0 50-51 34.63313365676616 38.5 34.5 39.5 25.0 40.5 52-53 34.89346159543737 38.5 35.0 40.0 26.0 40.5 54-55 35.224835197392785 39.0 35.0 41.0 26.0 41.0 56-57 35.139563736019554 39.0 35.0 41.0 25.5 41.0 58-59 34.94659654840382 39.0 35.0 41.0 24.0 41.0 60-61 34.704369120805865 39.0 35.0 41.0 23.0 41.0 62-63 34.40877759054885 38.5 35.0 40.0 22.0 41.0 64-65 34.12082669802237 38.0 34.5 40.0 21.0 41.0 66-67 33.74162562032442 38.0 34.0 40.0 18.5 41.0 68-69 33.308421598400116 37.0 34.0 40.0 13.0 41.0 70-71 32.97045820679949 37.0 33.5 39.0 10.0 41.0 72-73 32.54301533219761 36.0 33.0 39.0 7.5 41.0 74-75 32.07720261462114 36.0 32.5 39.0 2.0 40.0 76-77 31.643012554625585 35.0 32.0 37.5 2.0 39.5 78-79 31.178461317680174 35.0 32.0 37.0 2.0 39.0 80-81 30.696580345900305 35.0 31.0 36.5 2.0 39.0 82-83 30.25578660839938 35.0 31.0 36.0 2.0 38.5 84-85 29.816015943263462 35.0 31.0 36.0 2.0 37.0 86-87 29.490012313902675 35.0 30.5 35.0 2.0 37.0 88-89 29.076860047403898 34.0 30.0 35.0 2.0 36.0 90-91 28.70125824013036 34.0 29.0 35.0 2.0 36.0 92-93 28.333949800014814 34.0 29.0 35.0 2.0 36.0 94-95 27.94467076512851 34.0 29.0 35.0 2.0 35.0 96-97 27.531560162210205 34.0 28.5 35.0 2.0 35.0 98-99 27.04057338345308 34.0 27.0 35.0 2.0 35.0 100-101 25.054535312199096 32.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36045333982372085 1101 2 0.11294342759794418 1101 3 0.2516910861569066 1101 4 0.34598711443427277 1101 5 0.09847853399485729 1101 6 0.1408539911016149 1101 7 0.16866910213440178 1101 8 0.057344389313847444 1101 9 0.2491015017075071 1101 10-11 0.39001168278961984 1101 12-13 0.45922995692920665 1101 14-15 0.2872223531328828 1101 16-17 0.2842282189011982 1101 18-19 0.22805956166626373 1101 20-21 0.25252061333794273 1101 22-23 0.2992970683357399 1101 24-25 0.1250051175453777 1101 26-27 0.49742220708275653 1101 28-29 0.2609041727314789 1101 30-31 0.13539082681978698 1101 32-33 0.38190553526786175 1101 34-35 0.44224529454707806 1101 36-37 0.2916977128884781 1101 38-39 0.29030138722863086 1101 40-41 0.596337859682663 1101 42-43 0.2672986816939513 1101 44-45 0.7240862202505269 1101 46-47 0.5647262121401937 1101 48-49 0.8501923998288277 1101 50-51 0.9364114609672995 1101 52-53 1.1461119929284038 1101 54-55 1.0501446240589871 1101 56-57 1.26306264298519 1101 58-59 1.2756400267532015 1101 60-61 1.2558843012651693 1101 62-63 1.1900933070159283 1101 64-65 1.047072238311003 1101 66-67 1.478607952784543 1101 68-69 1.0510299049814904 1101 70-71 0.9519018565861117 1101 72-73 1.0680803532134249 1101 74-75 0.9073911005765467 1101 76-77 0.9429687261843043 1101 78-79 1.1933987495093454 1101 80-81 1.0897785815370327 1101 82-83 0.7236393563031207 1101 84-85 0.9980280086528026 1101 86-87 0.8047381415070909 1101 88-89 0.8252271601034984 1101 90-91 1.055787068501207 1101 92-93 1.0481103685980457 1101 94-95 0.8060920595788019 1101 96-97 0.8654427736837711 1101 98-99 1.0766630873892353 1101 100-101 1.108737708604039 1102 1 0.3573098411505242 1102 2 0.3176382632786918 1102 3 0.46728610554862016 1102 4 0.36525635305439863 1102 5 0.44189555787728096 1102 6 0.3000293330081263 1102 7 0.3068197451227661 1102 8 0.29045318702766565 1102 9 0.002112320241906218 1102 10-11 0.3691911071619387 1102 12-13 0.3645779871394481 1102 14-15 0.6855077174929605 1102 16-17 0.5441996416405317 1102 18-19 0.5311418233523213 1102 20-21 0.5411101214008127 1102 22-23 0.37094434328981407 1102 24-25 0.26013473583482494 1102 26-27 0.5802554383544489 1102 28-29 0.8243252790797158 1102 30-31 0.268336325901231 1102 32-33 0.29713313273673236 1102 34-35 0.3873769540694312 1102 36-37 0.7892278210738155 1102 38-39 0.5666230855344097 1102 40-41 0.4458581699500641 1102 42-43 0.5809749421552723 1102 44-45 0.8181054078398304 1102 46-47 0.5603171490660941 1102 48-49 0.6478041573303557 1102 50-51 0.5697447943006395 1102 52-53 0.8042871307116215 1102 54-55 0.9736188024627452 1102 56-57 1.2052753394538556 1102 58-59 0.9257727068919976 1102 60-61 0.7824407413549892 1102 62-63 0.9373478487591385 1102 64-65 0.7708321893214105 1102 66-67 0.6290468178590487 1102 68-69 0.4916504397845145 1102 70-71 0.4681500705073134 1102 72-73 0.6242550827500608 1102 74-75 0.5074115129055912 1102 76-77 0.6156978545778564 1102 78-79 0.6024618236061059 1102 80-81 0.4203358381200104 1102 82-83 0.5218634836760536 1102 84-85 0.07875877003267817 1102 86-87 -0.08103446514732582 1102 88-89 0.07062417990345793 1102 90-91 0.15505222465552393 1102 92-93 0.2783614402453196 1102 94-95 0.1368126147941524 1102 96-97 -0.16074624448320307 1102 98-99 0.2514946856745972 1102 100-101 1.0169026402227352 1103 1 -0.03606490005030594 1103 2 0.20314550965550282 1103 3 -0.007455095279539137 1103 4 0.3476580093690984 1103 5 0.2828893466971536 1103 6 0.38657177607230864 1103 7 0.1602358941910893 1103 8 0.1635380731560332 1103 9 0.006253106991387369 1103 10-11 0.2949675046774658 1103 12-13 0.1300024177812702 1103 14-15 0.1181164131451311 1103 16-17 0.38260543874198305 1103 18-19 0.5384917198326562 1103 20-21 0.2663689205726527 1103 22-23 0.14299403273080458 1103 24-25 0.5471947772426944 1103 26-27 0.5943341133026863 1103 28-29 0.5371617180031123 1103 30-31 0.5701996799384972 1103 32-33 0.6199074598588865 1103 34-35 0.08427136400731428 1103 36-37 0.1315673655872729 1103 38-39 -0.05149285649456914 1103 40-41 0.23633436042624822 1103 42-43 0.31565403118218427 1103 44-45 0.4468838757487319 1103 46-47 0.40503764596050473 1103 48-49 0.3111581945974393 1103 50-51 -0.1089301539395322 1103 52-53 -0.035153863077198366 1103 54-55 0.20488174242132828 1103 56-57 0.3815693353130669 1103 58-59 0.529292375629062 1103 60-61 0.6129790436324214 1103 62-63 0.6629172069371947 1103 64-65 0.48201231354501317 1103 66-67 0.4145540642358654 1103 68-69 0.8027270443393775 1103 70-71 1.0885434452485185 1103 72-73 0.7477540475533786 1103 74-75 0.6386744528641799 1103 76-77 0.596857274867709 1103 78-79 1.062710270811074 1103 80-81 1.1979755896728044 1103 82-83 1.038633670011457 1103 84-85 1.0674751261403372 1103 86-87 1.4182408971715148 1103 88-89 1.2487815297999383 1103 90-91 1.4885925973263276 1103 92-93 1.4407838004937688 1103 94-95 1.181947190363509 1103 96-97 1.263063279326321 1103 98-99 1.5077058657988225 1103 100-101 2.4605879404297752 1104 1 0.4787631989786476 1104 2 0.5756194839359701 1104 3 0.6383108044667658 1104 4 0.1572547200680745 1104 5 0.4928447311779536 1104 6 0.15212159673541237 1104 7 0.31092262326116327 1104 8 0.48551340339835036 1104 9 0.44276347353849843 1104 10-11 0.557969429268482 1104 12-13 0.4835257315770889 1104 14-15 0.5101096770765423 1104 16-17 0.5859887822814827 1104 18-19 0.6917919560324677 1104 20-21 0.7514642753097789 1104 22-23 0.972536504955464 1104 24-25 1.0200755400805903 1104 26-27 0.7674926296179692 1104 28-29 0.7718196656892289 1104 30-31 0.8790527986507755 1104 32-33 0.6685252535777266 1104 34-35 0.5690058579273582 1104 36-37 0.5793737692265069 1104 38-39 0.7438225139891941 1104 40-41 0.7290843385559 1104 42-43 0.864129667609447 1104 44-45 0.8392270653209479 1104 46-47 0.6700079022810428 1104 48-49 0.5727276110964326 1104 50-51 0.6645804750518138 1104 52-53 0.7897739647593838 1104 54-55 0.8747925113829282 1104 56-57 0.8796119387598367 1104 58-59 0.7492315760489774 1104 60-61 0.6689124702792526 1104 62-63 0.7496003492694499 1104 64-65 0.6851004073250877 1104 66-67 0.43353753024933894 1104 68-69 0.5284946937138812 1104 70-71 0.5656342509523071 1104 72-73 1.081812747579626 1104 74-75 0.999644621703311 1104 76-77 1.0324053459026175 1104 78-79 0.7722719889459704 1104 80-81 0.8175954829454852 1104 82-83 0.8549773844721322 1104 84-85 0.9448589945682997 1104 86-87 0.9378367330563897 1104 88-89 0.7357905403851852 1104 90-91 0.8343081952617659 1104 92-93 0.4850821995839638 1104 94-95 0.38214839760697217 1104 96-97 0.2344568581908142 1104 98-99 1.0660997071075435 1104 100-101 1.5770475677589673 1105 1 0.2745644214075611 1105 2 0.16795272868749933 1105 3 0.22309687940697032 1105 4 -0.05087708965820781 1105 5 0.21285536099458824 1105 6 0.2587386575911168 1105 7 0.2968115416773216 1105 8 0.24150596252670908 1105 9 4.169415161712209E-4 1105 10-11 0.2366234287369977 1105 12-13 0.30113800901530396 1105 14-15 0.5331697355405396 1105 16-17 0.8378556438281137 1105 18-19 0.7094573825538575 1105 20-21 0.7980693776217649 1105 22-23 0.5935038182693049 1105 24-25 0.8347313997670085 1105 26-27 0.2981662942472596 1105 28-29 0.7719326074909816 1105 30-31 0.6912239386986059 1105 32-33 1.0452326678748278 1105 34-35 0.5672785127240658 1105 36-37 0.6269089804941075 1105 38-39 1.0600329843585783 1105 40-41 1.2916607403629712 1105 42-43 1.3577318467218547 1105 44-45 1.5860846257780352 1105 46-47 1.831933227819171 1105 48-49 1.8090018603656262 1105 50-51 1.889241649646543 1105 52-53 2.188126343180862 1105 54-55 2.1836816955445 1105 56-57 2.1859698978350366 1105 58-59 2.2234210525305045 1105 60-61 2.08968711816658 1105 62-63 1.9871701074411234 1105 64-65 2.3820709095839234 1105 66-67 2.5035614473367644 1105 68-69 2.7213195674848123 1105 70-71 2.5083196083798853 1105 72-73 2.318731429482355 1105 74-75 2.334045365790473 1105 76-77 1.944191155097407 1105 78-79 2.1863830705298106 1105 80-81 2.3515909652734166 1105 82-83 2.876416395899188 1105 84-85 3.006212967462261 1105 86-87 2.98464778806569 1105 88-89 2.925067712853963 1105 90-91 2.781139181177263 1105 92-93 2.851618208087814 1105 94-95 2.6622706404982814 1105 96-97 2.618963900444335 1105 98-99 2.6610106823932185 1105 100-101 2.8874249262495333 1106 1 0.4849959980720584 1106 2 0.44752332074995493 1106 3 0.6941226872577637 1106 4 0.5674052785916288 1106 5 0.3988670721201615 1106 6 0.41827021256834485 1106 7 0.37948341328868196 1106 8 0.15647017853191159 1106 9 0.3564351588226131 1106 10-11 0.4456778637836294 1106 12-13 0.6672516503078683 1106 14-15 0.5647680873080532 1106 16-17 0.8060187320953034 1106 18-19 0.9171238323478264 1106 20-21 0.8237687920654864 1106 22-23 0.7079258525351904 1106 24-25 0.5503896083985467 1106 26-27 0.9431240040715849 1106 28-29 0.5010369232576295 1106 30-31 0.6769320280501603 1106 32-33 0.8542545720170978 1106 34-35 0.596122581255834 1106 36-37 0.6872538080803139 1106 38-39 0.6692717611965762 1106 40-41 0.6430595692504113 1106 42-43 0.3242033279623726 1106 44-45 0.6896196507183916 1106 46-47 0.6605170491160663 1106 48-49 0.6210925131524476 1106 50-51 0.32031950694431544 1106 52-53 0.571828360096923 1106 54-55 0.6149981128075694 1106 56-57 0.4601728906782441 1106 58-59 0.529970607941479 1106 60-61 0.49485953195968335 1106 62-63 0.7661334559555399 1106 64-65 0.9486182962679379 1106 66-67 1.0252487169095303 1106 68-69 0.5180122588749683 1106 70-71 0.6384649130860325 1106 72-73 0.8243050577625581 1106 74-75 1.1704799786727094 1106 76-77 1.1205954058022485 1106 78-79 1.2841709690333936 1106 80-81 1.472909551263438 1106 82-83 1.6119184561897981 1106 84-85 1.519713292771307 1106 86-87 1.5018241055673194 1106 88-89 1.333167908039389 1106 90-91 1.5805644685335842 1106 92-93 1.6021495461923472 1106 94-95 1.5255432494768115 1106 96-97 1.4913776935477827 1106 98-99 1.7547430614866926 1106 100-101 2.2092314758049447 1107 1 -0.1300089994291902 1107 2 -0.10663709904014951 1107 3 0.17888030844717306 1107 4 -0.3394537918731402 1107 5 -0.13636531168793908 1107 6 -0.35178226119477785 1107 7 0.10355887555755316 1107 8 0.1705256507957813 1107 9 0.2593586970535 1107 10-11 0.23169110716193586 1107 12-13 0.09700552337133672 1107 14-15 0.20235554357991248 1107 16-17 0.04021413439415511 1107 18-19 0.3548737074102988 1107 20-21 0.24074780256023587 1107 22-23 0.5948573867680764 1107 24-25 0.5130332865594625 1107 26-27 0.5108713803834348 1107 28-29 0.5946151341521784 1107 30-31 0.3411624128577557 1107 32-33 0.5989447269396351 1107 34-35 0.5710726062433409 1107 36-37 0.6486481109288889 1107 38-39 0.7148114913315098 1107 40-41 0.8791915032833941 1107 42-43 0.8226416088219395 1107 44-45 1.2787938136369306 1107 46-47 1.2396649751530475 1107 48-49 1.0999780703738367 1107 50-51 0.7124984174890372 1107 52-53 0.9586349567985764 1107 54-55 1.1381115560859243 1107 56-57 1.0849854843813915 1107 58-59 0.8813886489209892 1107 60-61 1.2880566833839708 1107 62-63 1.409811616875082 1107 64-65 1.4610495806257617 1107 66-67 1.4227062381489048 1107 68-69 0.989838845581609 1107 70-71 0.8831863023913726 1107 72-73 1.0447260972428225 1107 74-75 0.8001651360939945 1107 76-77 0.2925094487807556 1107 78-79 0.517679214910455 1107 80-81 0.617618446815662 1107 82-83 0.4147982662847518 1107 84-85 0.22586021930803923 1107 86-87 0.22530611456281946 1107 88-89 0.24761693352664338 1107 90-91 0.16755222465551967 1107 92-93 0.7191223098105368 1107 94-95 0.749493774214443 1107 96-97 0.5472247700095529 1107 98-99 0.4091033813267728 1107 100-101 1.1803084373241859 1108 1 -0.34705340275943897 1108 2 -0.1724326587071232 1108 3 0.11366291714282184 1108 4 -0.09326970306647553 1108 5 -0.07600453462966072 1108 6 0.26951746128440845 1108 7 -0.34752808096418875 1108 8 -0.6221431743661086 1108 9 -0.46381429092059534 1108 10-11 -0.44806143677885757 1108 12-13 -0.4641068725583608 1108 14-15 -0.13234612154496972 1108 16-17 0.01627796418139127 1108 18-19 -0.009661445226150533 1108 20-21 0.030757053254042432 1108 22-23 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-0.05766347612043177 2316 78-79 -0.6204136704650409 2316 80-81 -0.6241058614847361 2316 82-83 -1.2599794016994394 2316 84-85 -0.44002416804373823 2316 86-87 0.03150670744819095 2316 88-89 -0.38812911390418847 2316 90-91 -0.2592017279136485 2316 92-93 -0.6063470577783932 2316 94-95 -0.9134261862598656 2316 96-97 -0.1895588268283923 2316 98-99 -0.801617962546743 2316 100-101 -1.1502251595137594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 681.0 3 136.0 4 146.0 5 141.0 6 147.0 7 179.0 8 214.0 9 276.0 10 380.0 11 511.0 12 743.0 13 994.0 14 1120.0 15 1553.0 16 2139.0 17 2931.0 18 2433.0 19 1472.0 20 1331.0 21 1295.0 22 1510.0 23 1648.0 24 1709.0 25 1931.0 26 2108.0 27 2442.0 28 2838.0 29 3310.0 30 4096.0 31 5234.0 32 6495.0 33 8432.0 34 11993.0 35 18029.0 36 24132.0 37 40338.0 38 50152.0 39 10797.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.584462025902795 24.766078078968334 17.913323822052586 20.73613607307628 2 13.429508865264662 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33.37733651082594 18.41903531139196 46-47 16.985562283636867 31.574023688506557 33.47165136446261 17.968762663393964 48-49 16.94830864286161 31.79617102773654 32.98904850016095 18.2664718292409 50-51 16.737440205235544 31.581823317758523 32.70038481664482 18.98035166036111 52-53 16.84424549646039 32.45373726052331 32.63297570554875 18.06904153746755 54-55 17.551469396262245 31.81075009842292 32.26372710219773 18.37405340311711 56-57 17.596954159545348 32.22703924483907 31.461410553911286 18.714596041704297 58-59 16.874460057578418 31.26535868389541 32.96445470737147 18.895726551154695 60-61 17.72189424486591 32.48133848729998 31.717739264563853 18.079028003270253 62-63 17.069719071585908 32.50121019949183 32.62836800255705 17.80070272636521 64-65 17.593349465664765 31.45364656300293 32.375041971077586 18.577962000254722 66-67 17.15208257088987 32.18175416815295 31.595039446315486 19.071123814641695 68-69 17.012564414336172 31.54119622488565 32.64755949279138 18.7986798679868 70-71 17.91545500009261 31.136666728407636 32.45962140436014 18.4882568671396 72-73 17.301174248036748 31.19026892873018 32.167497036598014 19.341059786635057 74-75 17.24798214376676 32.09583833070152 31.46674878558165 19.18943073995008 76-77 16.814021989829474 31.511268166618812 32.55680397187821 19.117905871673504 78-79 16.84324389430382 30.99567320164548 32.32544565096542 19.835637253085274 80-81 17.52472037607392 32.01143968691383 31.161568209712154 19.302271727300095 82-83 17.827728367611638 31.1967296646285 31.553409301463773 19.42213266629609 84-85 17.072747024174888 30.803119549532234 31.382881390872512 20.74125203542037 86-87 17.289652520741747 31.572388809880064 31.69723304411472 19.440725625263468 88-89 17.74804120080993 30.43957724225168 31.740655450581734 20.071726106356657 90-91 17.700412510104297 29.825569141186854 32.35783919749298 20.116179151215874 92-93 18.4641329098823 30.825752679578677 31.373829892214573 19.33628451832445 94-95 17.833934516238887 29.69241212188607 32.21089357325049 20.262759788624553 96-97 17.94197512557691 29.994346722025544 31.667624325777094 20.396053826620452 98-99 17.873846396306615 30.819933530177952 31.377788696422034 19.928431377093407 100-101 17.913097489524233 30.307876293247304 31.50401416546149 20.275012051766975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2499.0 1 1526.5 2 504.5 3 427.5 4 344.5 5 342.5 6 494.5 7 840.0 8 1141.5 9 1112.5 10 868.5 11 702.0 12 699.0 13 667.5 14 635.0 15 628.0 16 615.0 17 610.5 18 598.0 19 637.0 20 710.5 21 762.5 22 827.5 23 958.5 24 1074.0 25 1265.5 26 1792.5 27 2326.0 28 2393.0 29 2815.5 30 4084.0 31 5150.0 32 6716.5 33 8893.5 34 10246.5 35 11592.5 36 14191.0 37 15885.0 38 14938.5 39 13295.0 40 11675.5 41 9738.5 42 7685.5 43 7274.0 44 7554.5 45 6907.0 46 5526.0 47 4609.0 48 4893.0 49 4161.0 50 2764.0 51 1746.5 52 1204.0 53 954.5 54 791.0 55 695.5 56 573.5 57 500.5 58 446.0 59 412.5 60 386.5 61 338.5 62 241.5 63 129.0 64 67.0 65 47.5 66 38.5 67 29.0 68 15.0 69 9.5 70 7.5 71 6.5 72 7.0 73 6.0 74 4.5 75 1.5 76 0.5 77 1.0 78 1.0 79 2.0 80 1.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06110658469742983 2 0.10647359454855196 3 0.07082808680838457 4 0.14304495963261982 5 0.1180468113473076 6 0.1055477372046515 7 0.1231390267387601 8 0.10601066587660174 9 0.12499074142656098 10-11 0.12012999037108363 12-13 0.10253870083697504 14-15 0.10693652322050218 16-17 0.1215187763869343 18-19 0.09721502110954744 20-21 0.0805495889193393 22-23 0.0803181245833642 24-25 0.06874490778460855 26-27 0.05994926301755426 28-29 0.07476298051996148 30-31 0.056014369305977335 32-33 0.050459225242574623 34-35 0.04629286719502259 36-37 0.05346826161025109 38-39 0.05670876231390268 40-41 0.07105955114435968 42-43 0.06874490778460855 44-45 0.05670876231390268 46-47 0.040969187467594995 48-49 0.05069068957854973 50-51 0.031942078364565585 52-53 0.04745018887489816 54-55 0.051385082586475075 56-57 0.05323679727427598 58-59 0.06318976372120584 60-61 0.06041219168950448 62-63 0.0659673357529072 64-65 0.043283830827346124 66-67 0.07082808680838457 68-69 0.059486334345604026 70-71 0.027775720317013555 72-73 0.022220576253610843 74-75 0.031942078364565585 76-77 0.04536700985112214 78-79 0.07036515813643435 80-81 0.046755795866972816 82-83 0.06295829938523073 84-85 0.07013369380045922 86-87 0.06805051477668321 88-89 0.09027109103029404 90-91 0.0659673357529072 92-93 0.07892933856751352 94-95 0.08865084067846826 96-97 0.09814087845344789 98-99 0.12915709947411302 100-101 0.12962002814606324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 216016.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.17661654692245 #Duplication Level Percentage of deduplicated Percentage of total 1 75.30641080406139 21.218798607510557 2 9.149607334143857 5.156099548181616 3 3.504419544573325 2.9622805718094956 4 2.0553346696020767 2.3164950744389303 5 1.3439358591003188 1.893378268276424 6 0.9808431636710151 1.6582105029257093 7 0.7902605724049552 1.5586808384564106 8 0.6473236289554102 1.4591511739871121 9 0.5848914007820458 1.4832234649285239 >10 4.6397003253047675 27.818309754832978 >50 0.6867545099070088 13.702225761054738 >100 0.30230342062892257 13.91239537812014 >500 0.0 0.0 >1k 0.008214766864916373 4.860751055477372 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3004 1.3906377305384787 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2306 1.0675135175172208 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2181 1.0096474335234427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 0.8503999703725651 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1172 0.5425524035256648 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 444 0.2055403303459003 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 423 0.19581882823494554 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 416 0.19257832753129397 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 386 0.1786904673727872 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 382 0.1768387526849863 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 364 0.16850603658988222 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 354 0.16387674987037998 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 339 0.15693281979112658 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 319 0.14767424635212206 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 315 0.14582253166432116 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 295 0.13656395822531664 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 295 0.13656395822531664 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 274 0.1268424561143619 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 266 0.1231390267387601 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 260 0.12036145470705874 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 259 0.11989852603510852 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 258 0.1194355973631583 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 257 0.11897266869120805 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 255 0.1180468113473076 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 255 0.1180468113473076 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 253 0.11712095400340716 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 241 0.11156580994000445 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 237 0.10971409525220353 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 235 0.1087882379083031 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 231 0.10693652322050218 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 230 0.10647359454855196 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 223 0.10323309384490037 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 217 0.10045552181319901 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.3146433597511296E-4 0.0 18-19 0.0 0.0 0.0 6.943930079253388E-4 0.0 20-21 0.0 0.0 0.0 0.0013887860158506777 0.0 22-23 0.0 0.0 0.0 0.0027775720317013554 0.0 24-25 0.0 0.0 0.0 0.005786608399377824 0.0 26-27 0.0 0.0 0.0 0.009258573439004518 0.0 28-29 0.0 0.0 0.0 0.017128360862158357 0.0 30-31 0.0 0.0 0.0 0.028007184652988668 0.0 32-33 0.0 0.0 0.0 0.041432116139545214 4.629286719502259E-4 34-35 0.0 0.0 0.0 0.05832901266572846 4.629286719502259E-4 36-37 0.0 0.0 0.0 0.07360565884008591 4.629286719502259E-4 38-39 0.0 0.0 0.0 0.09397452040589586 4.629286719502259E-4 40-41 0.0 0.0 0.0 0.11295459595585512 4.629286719502259E-4 42-43 0.0 0.0 0.0 0.16364528553440488 4.629286719502259E-4 44-45 0.0 0.0 0.0 0.21780794015258131 4.629286719502259E-4 46-47 0.0 0.0 0.0 0.26178616398785276 4.629286719502259E-4 48-49 0.0 0.0 0.0 0.32775349974075996 4.629286719502259E-4 50-51 0.0 0.0 0.0 0.4133953040515517 4.629286719502259E-4 52-53 0.0 0.0 0.0 0.555282942004296 4.629286719502259E-4 54-55 0.0 0.0 0.0 0.6923098289015628 4.629286719502259E-4 56-57 0.0 0.0 0.0 0.8971557662395377 4.629286719502259E-4 58-59 0.0 0.0 0.0 1.1663487889785942 4.629286719502259E-4 60-61 0.0 0.0 0.0 1.4105436634323385 4.629286719502259E-4 62-63 0.0 0.0 0.0 1.7144563365676615 4.629286719502259E-4 64-65 0.0 0.0 0.0 1.9683727131323607 4.629286719502259E-4 66-67 0.0 0.0 0.0 2.3498259388193468 4.629286719502259E-4 68-69 0.0 0.0 0.0 2.7808125324050073 4.629286719502259E-4 70-71 0.0 0.0 0.0 3.1564791496926157 4.629286719502259E-4 72-73 0.0 0.0 0.0 3.6589882230945854 4.629286719502259E-4 74-75 0.0 0.0 0.0 4.155247759425228 4.629286719502259E-4 76-77 0.0 0.0 0.0 4.551746166950596 4.629286719502259E-4 78-79 0.0 0.0 0.0 4.968613436041775 4.629286719502259E-4 80-81 0.0 0.0 0.0 5.566022887193541 4.629286719502259E-4 82-83 0.0 0.0 0.0 6.0926042515369225 4.629286719502259E-4 84-85 0.0 0.0 0.0 6.888147174283386 4.629286719502259E-4 86-87 0.0 0.0 0.0 7.781599511147322 4.629286719502259E-4 88-89 0.0 0.0 0.0 8.793098659358566 4.629286719502259E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTACAC 25 0.004998334 57.050125 3 CTTGGAC 25 0.0050029284 57.036892 2 ATGGGAC 70 2.0372713E-4 33.95841 5 GGTATCA 1250 0.0 31.560413 1 GTACATG 690 0.0 29.620605 1 CATGGGT 65 0.0058086724 29.256474 4 ACATGGG 710 0.0 28.123302 3 GTATCAA 1765 0.0 27.737488 1 AGTCTGC 70 0.008350319 27.160423 8 CTCCTCG 70 0.008350319 27.160423 9 CATGGGG 230 3.6379788E-12 26.871437 4 CATGGGA 375 0.0 26.623394 4 CACTGAC 155 1.3910812E-6 24.53769 7 ATCAACG 2075 0.0 23.59905 3 TCAACGC 2065 0.0 23.483103 4 CAACGCA 2070 0.0 23.426382 5 CCTTGGC 145 2.3113877E-5 22.945875 9 AACGCAG 2125 0.0 22.820051 6 GAGTACT 1240 0.0 22.799156 12-13 TATCAAC 2205 0.0 22.20257 2 >>END_MODULE