FastQCFastQC Report
Thu 26 May 2016
SRR937119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216016
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29121.3480482927190578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28301.3100881416191394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22651.0485334419672616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.9619657803125694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.6221761351011036No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5610.25970298496407673No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.23979705207021704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.22729797792756093No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4370.2022998296422487No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG3470.1606362491667284No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3410.15785867713502705No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3360.1555440337752759No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC3260.15091474705577365No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3220.14906303236797275No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3140.14535960299237094No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3060.14165617361676913No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG2900.13424931486556552No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA2780.1286941708021628No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT2760.12776831345826234No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA2740.1268424561143619No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA2730.12637952744241168No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2720.12591659877046146No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT2650.12267609806680987No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA2610.12082438337900896No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC2570.11897266869120805No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.11526923931560625No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG2420.11202873861195467No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG2410.11156580994000445No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT2230.10323309384490037No Hit
ATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTC2230.10323309384490037No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA2220.10277016517295015No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC2210.10230723650099993No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA2200.10184430782904971No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC2190.10138137915709947No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2180.10091845048514925No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.10091845048514925No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.10045552181319901No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGT352.7849092E-454.277647
AGCTCCG250.001614042138.0031546-47
GATCCTC500.0016140937.994355
TGGACTG651.3257755E-436.5330285
GGGTCCT651.3257755E-436.5330287
TGGGTAT651.3257755E-436.5330286
GGACTGG651.3257755E-436.5330286
GGGGTCC651.3257755E-436.5330286
AGGGGTC702.049625E-433.9235275
CCTCATC902.4064117E-531.661968
GGTTACT600.00394678531.661968
GGTCCTA753.072917E-431.6619598
ATGGGTA953.481402E-529.995545
CGGCTCG404.480789E-429.69683536-37
GTAGATA650.005837948529.2264236
ACTGGTA650.005837948529.2264238
CATGGGT1153.8499456E-628.9087474
CCCTATA1004.9397633E-528.4957642
CGCAGAA1004.9397633E-528.4957649
ACCCTTG1703.612513E-927.9370237
Produced by FastQC (version 0.11.5)