##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937119_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 216016 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.900650877712764 31.0 31.0 34.0 30.0 34.0 2 31.942601473964892 31.0 31.0 34.0 30.0 34.0 3 32.08160043700467 33.0 31.0 34.0 30.0 34.0 4 35.88153192356122 37.0 35.0 37.0 35.0 37.0 5 35.791751536923194 37.0 35.0 37.0 35.0 37.0 6 35.841187689800755 37.0 35.0 37.0 35.0 37.0 7 35.773674172283535 37.0 35.0 37.0 33.0 37.0 8 35.7631518035701 37.0 35.0 37.0 33.0 37.0 9 37.40440059995556 39.0 37.0 39.0 34.0 39.0 10-11 37.339539663728615 39.0 37.0 39.0 34.0 39.0 12-13 37.272563143470855 39.0 37.0 39.0 34.0 39.0 14-15 38.37814328568254 40.0 38.0 41.0 33.5 41.0 16-17 38.27676190652544 40.0 38.0 41.0 33.0 41.0 18-19 38.31227779423746 40.0 37.5 41.0 33.5 41.0 20-21 38.392952373898225 40.0 38.0 41.0 34.0 41.0 22-23 38.27564161913932 40.0 37.5 41.0 34.0 41.0 24-25 38.16671450262943 40.0 37.0 41.0 33.0 41.0 26-27 37.97305986593585 40.0 37.0 41.0 33.0 41.0 28-29 37.78761758388268 40.0 37.0 41.0 33.0 41.0 30-31 37.58508860454781 39.0 36.0 41.0 33.0 41.0 32-33 37.4302135027035 39.0 36.0 41.0 33.0 41.0 34-35 37.29586465817347 39.0 35.0 41.0 33.0 41.0 36-37 37.08296376194356 39.0 35.0 41.0 32.0 41.0 38-39 36.905606529145984 39.0 35.0 41.0 31.5 41.0 40-41 36.72253907117991 39.0 35.0 41.0 31.0 41.0 42-43 36.456399063032364 39.0 35.0 41.0 31.0 41.0 44-45 36.02037580549589 39.0 35.0 41.0 30.0 41.0 46-47 35.67974825938819 39.0 35.0 40.5 28.5 41.0 48-49 35.277423894526336 39.0 35.0 40.0 26.5 41.0 50-51 34.978897396489145 39.0 35.0 40.0 25.0 41.0 52-53 34.76908886378787 38.5 35.0 40.0 24.0 41.0 54-55 34.55107954966299 38.0 34.5 40.0 23.5 41.0 56-57 34.286358417894974 38.0 34.0 40.0 22.5 41.0 58-59 33.97408525294422 38.0 34.0 40.0 22.0 41.0 60-61 33.66456882823495 38.0 33.0 40.0 19.5 41.0 62-63 33.29545496629879 37.5 33.0 40.0 18.5 41.0 64-65 32.91369389304496 37.0 33.0 39.5 13.5 41.0 66-67 32.538346696541 36.5 32.0 39.0 8.5 40.5 68-69 32.07654294126361 36.0 31.5 39.0 7.5 40.0 70-71 31.59347918672691 35.5 31.0 38.5 3.5 40.0 72-73 31.220911876898008 35.0 31.0 38.0 2.0 40.0 74-75 30.754696411376933 35.0 30.5 37.0 2.0 39.0 76-77 29.315006758758614 33.5 28.5 36.0 2.0 38.5 78-79 30.00727260943634 34.0 30.0 36.0 2.0 39.0 80-81 29.777815069254128 34.0 30.0 36.0 2.0 38.5 82-83 29.382659154877416 34.0 29.0 36.0 2.0 37.0 84-85 28.93268554181172 34.0 29.0 35.0 2.0 37.0 86-87 28.544415691430267 34.0 29.0 35.0 2.0 36.0 88-89 28.126828568254204 34.0 29.0 35.0 2.0 36.0 90-91 27.680558384564108 34.0 27.5 35.0 2.0 36.0 92-93 27.198323272350194 33.0 26.0 35.0 2.0 35.0 94-95 26.687250018517148 33.0 25.5 35.0 2.0 35.0 96-97 26.024016739500777 33.0 24.5 35.0 2.0 35.0 98-99 25.3584433560477 32.0 21.5 34.0 2.0 35.0 100-101 22.58044311532479 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0896238792545283 1101 2 0.1807378967238371 1101 3 0.014859490756471416 1101 4 0.16892840847638269 1101 5 0.2050709350651303 1101 6 -0.031155078686374793 1101 7 0.055725218412192135 1101 8 0.14655665006648633 1101 9 0.40836067058420156 1101 10-11 0.24406084003171458 1101 12-13 0.16154094147882603 1101 14-15 0.13917380660308964 1101 16-17 0.21651913900950603 1101 18-19 0.32145617915703895 1101 20-21 0.3243476589272234 1101 22-23 0.06858891182429971 1101 24-25 0.2559272195846134 1101 26-27 0.40031583724839237 1101 28-29 0.5902827644134874 1101 30-31 0.6309959607216769 1101 32-33 0.7190316249298689 1101 34-35 0.35742294300685984 1101 36-37 0.7132978462007742 1101 38-39 0.6619202204813988 1101 40-41 0.597385014171202 1101 42-43 0.9920740596668338 1101 44-45 0.9437722179576085 1101 46-47 0.958731811998355 1101 48-49 0.9472579551332174 1101 50-51 0.5430349901631359 1101 52-53 0.7931520582509393 1101 54-55 0.7298618679088875 1101 56-57 1.014773853467382 1101 58-59 0.9043353080889034 1101 60-61 0.9539066393083644 1101 62-63 0.9807514218838875 1101 64-65 0.857235452265158 1101 66-67 1.200691032365576 1101 68-69 1.083927898903017 1101 70-71 1.3799837406527153 1101 72-73 1.5047968203584219 1101 74-75 1.061756029907798 1101 76-77 1.1152204271715185 1101 78-79 1.3355923551370061 1101 80-81 1.5068630421983826 1101 82-83 1.2493848227362676 1101 84-85 1.3296356449425808 1101 86-87 0.8772110202792582 1101 88-89 1.038678919796535 1101 90-91 1.676411541577 1101 92-93 1.8194807900094183 1101 94-95 1.7755020155556025 1101 96-97 1.6068745650120206 1101 98-99 1.9103900111122947 1101 100-101 1.6192264787336441 1102 1 0.345880258107357 1102 2 0.10733516147174171 1102 3 0.30528610843354187 1102 4 0.24776082325490734 1102 5 0.22801439291367132 1102 6 0.16876327199744168 1102 7 0.33978318942669006 1102 8 0.20640559883153742 1102 9 0.38386587572811237 1102 10-11 0.40621638197905696 1102 12-13 0.2555397167390865 1102 14-15 0.3301719694934775 1102 16-17 0.34632113941775344 1102 18-19 0.2601987796877623 1102 20-21 0.19997533804540524 1102 22-23 0.43203043050158385 1102 24-25 0.37978923224025607 1102 26-27 0.26696209158601647 1102 28-29 0.5493764570038167 1102 30-31 0.3131759974638655 1102 32-33 0.3583457706739068 1102 34-35 0.20684119162902448 1102 36-37 0.487251714337134 1102 38-39 0.579617709764932 1102 40-41 0.6401488435241305 1102 42-43 0.7496776522367412 1102 44-45 0.640730780929637 1102 46-47 0.9759598177138074 1102 48-49 0.7682010047351682 1102 50-51 1.038360566811427 1102 52-53 1.23264991495639 1102 54-55 1.132168461091176 1102 56-57 1.2041186177049852 1102 58-59 1.1150517808384492 1102 60-61 1.2414755309189047 1102 62-63 1.6055932263337809 1102 64-65 1.3425385753514973 1102 66-67 1.2932201199344675 1102 68-69 1.5531461067005274 1102 70-71 1.4889447531042315 1102 72-73 1.6938353996603155 1102 74-75 1.7526725434819959 1102 76-77 1.7109338380716999 1102 78-79 1.8491053169659502 1102 80-81 1.766364981369641 1102 82-83 1.5160310770738867 1102 84-85 1.6334731628033694 1102 86-87 1.1276498853537653 1102 88-89 1.1709099873613447 1102 90-91 1.6269524682967393 1102 92-93 1.7714097551043366 1102 94-95 1.8434342466231683 1102 96-97 1.6102221869756903 1102 98-99 1.8725659653886808 1102 100-101 1.9330660378273379 1103 1 -0.11519634644751164 1103 2 -0.5359360600603509 1103 3 -0.2533060240716303 1103 4 0.009458545822191411 1103 5 -0.15604357810082092 1103 6 0.007065549430151918 1103 7 -0.12626235922755313 1103 8 0.051954253075848555 1103 9 -0.2596123851414518 1103 10-11 0.09752072980514015 1103 12-13 0.17075710804343203 1103 14-15 0.09414712477298082 1103 16-17 0.10667310629146698 1103 18-19 0.1916687589838304 1103 20-21 0.11550328835596702 1103 22-23 0.18668881559474926 1103 24-25 0.2180915096729663 1103 26-27 0.1674796899296993 1103 28-29 0.45682987315288415 1103 30-31 0.6665921465321887 1103 32-33 0.7294637830962643 1103 34-35 0.5400710052936191 1103 36-37 0.6454297681673609 1103 38-39 0.3799282687711383 1103 40-41 0.6127161313088152 1103 42-43 0.6799053955079657 1103 44-45 0.957397447596307 1103 46-47 1.2857941862438267 1103 48-49 1.3952196382755417 1103 50-51 1.114551043001903 1103 52-53 1.2149480516023559 1103 54-55 1.072955210573575 1103 56-57 1.398528555593181 1103 58-59 1.6215735199688766 1103 60-61 1.7445811209810174 1103 62-63 1.3013489199155615 1103 64-65 1.22142056292914 1103 66-67 1.4307977596860226 1103 68-69 1.516086065292658 1103 70-71 1.470828811075247 1103 72-73 1.9078105549398217 1103 74-75 1.5350741997966928 1103 76-77 1.5103127200592787 1103 78-79 1.4843020043365485 1103 80-81 1.753114463771297 1103 82-83 1.7997784890821684 1103 84-85 1.5400984216025435 1103 86-87 1.384896262165359 1103 88-89 1.8155269645870185 1103 90-91 1.9765383896217905 1103 92-93 1.706192363799989 1103 94-95 1.739604018879895 1103 96-97 2.2904499302469077 1103 98-99 2.118114619632987 1103 100-101 2.1802710067714344 1104 1 0.35300416094467124 1104 2 0.20116043193510436 1104 3 0.32964822315083353 1104 4 0.1985058732651126 1104 5 0.05507093506513172 1104 6 0.18222520300376743 1104 7 0.08671113390515472 1104 8 0.19373974865803234 1104 9 0.25554376917575183 1104 10-11 0.16870872735565712 1104 12-13 0.3214000964084036 1104 14-15 0.4603005671664704 1104 16-17 0.44504026577007494 1104 18-19 0.383428010142957 1104 20-21 0.12117864484271479 1104 22-23 0.08267341886654833 1104 24-25 0.08691313507756604 1104 26-27 -0.10919120500512491 1104 28-29 0.60260670807547 1104 30-31 0.5257142705808278 1104 32-33 0.3158626108453717 1104 34-35 0.49791590075333403 1104 36-37 0.3816077053557052 1104 38-39 0.4091033190729476 1104 40-41 0.0868216338895138 1104 42-43 0.6202430737513396 1104 44-45 0.7043355982392967 1104 46-47 0.9661261781955375 1104 48-49 0.9229621804853281 1104 50-51 0.8969082295997524 1104 52-53 1.0508985371241764 1104 54-55 0.6087351073455096 1104 56-57 0.5475203323406248 1104 58-59 0.6860254489339752 1104 60-61 0.6609488928294951 1104 62-63 0.78356832329235 1104 64-65 0.5174467198707831 1104 66-67 0.622874130957122 1104 68-69 1.1279419834100644 1104 70-71 0.4567443040329948 1104 72-73 0.7790925950063077 1104 74-75 0.8170377200486421 1104 76-77 0.3764880328053195 1104 78-79 0.4186909466863007 1104 80-81 0.8980602252969732 1104 82-83 1.0088918649897884 1104 84-85 1.0380863491679335 1104 86-87 0.6074927104201038 1104 88-89 0.750298638106397 1104 90-91 1.033805907774184 1104 92-93 1.0677202266291381 1104 94-95 0.9184597620344768 1104 96-97 1.0304661143077993 1104 98-99 1.4649674759010303 1104 100-101 1.1216912674660406 1105 1 0.28840145034142495 1105 2 0.05275490443045072 1105 3 0.2555185393878787 1105 4 0.10015349484363867 1105 5 -0.18270477580385602 1105 6 0.03203918121264593 1105 7 0.07836672784615928 1105 8 0.025164144087213458 1105 9 0.33644996104394664 1105 10-11 0.4496946428486197 1105 12-13 0.5346209308490089 1105 14-15 0.6355862434885537 1105 16-17 0.4185388041702822 1105 18-19 0.3546477816018978 1105 20-21 0.3048153708591883 1105 22-23 0.39495085708074384 1105 24-25 0.5223104244743269 1105 26-27 0.5585672324118249 1105 28-29 0.258154143632197 1105 30-31 0.4089922402858548 1105 32-33 0.3985426533646361 1105 34-35 0.5421160070515043 1105 36-37 0.45905920681730805 1105 38-39 0.48814929302989185 1105 40-41 0.34373101470269773 1105 42-43 0.5387123801558005 1105 44-45 0.3722867010827713 1105 46-47 0.39266750160771124 1105 48-49 0.3152887816014598 1105 50-51 0.529774293910144 1105 52-53 0.46349486983744015 1105 54-55 0.7587749691578907 1105 56-57 0.6087945816098213 1105 58-59 0.5846169982297482 1105 60-61 1.044100633461973 1105 62-63 0.6567280362872978 1105 64-65 0.3812588378617505 1105 66-67 0.913154492370893 1105 68-69 0.44967331479459816 1105 70-71 0.8113948488110942 1105 72-73 0.9046639476504765 1105 74-75 1.1553249908432228 1105 76-77 1.2140511473415962 1105 78-79 0.8335461154346397 1105 80-81 1.0757442539974775 1105 82-83 1.3434852745034753 1105 84-85 1.3123356183680386 1105 86-87 1.124806024265439 1105 88-89 0.8936882208860943 1105 90-91 1.2685986263497888 1105 92-93 1.9052899848008096 1105 94-95 1.8903306097623513 1105 96-97 1.8517589657561118 1105 98-99 1.7539988338601056 1105 100-101 2.4964786711333247 1106 1 0.3901581191768173 1106 2 0.31428168820837143 1106 3 0.460658422276623 1106 4 0.2354169951689471 1106 5 0.20777451285370319 1106 6 0.15442044341173755 1106 7 0.34767924140069795 1106 8 0.040388607327294324 1106 9 0.5499828172573444 1106 10-11 0.2985452175612622 1106 12-13 0.27433032143674296 1106 14-15 0.3980130489537501 1106 16-17 0.5239823088330482 1106 18-19 0.7043766907322393 1106 20-21 0.3720892810738974 1106 22-23 0.42640824159602886 1106 24-25 0.5263659438844286 1106 26-27 0.2435487982326876 1106 28-29 0.6463779562541845 1106 30-31 0.6021065321965082 1106 32-33 0.613843022048215 1106 34-35 0.7680106069213863 1106 36-37 0.5320043380252883 1106 38-39 0.868023506866372 1106 40-41 0.8921478440238815 1106 42-43 1.1156696562347435 1106 44-45 0.9234144391005472 1106 46-47 0.9270092929761731 1106 48-49 0.6459871116816984 1106 50-51 0.8433830555670525 1106 52-53 0.45748749616578976 1106 54-55 0.9991349778328029 1106 56-57 1.048471441293188 1106 58-59 0.8966859637469895 1106 60-61 1.0005959706990168 1106 62-63 1.272559743075412 1106 64-65 1.4045075908437497 1106 66-67 1.2957938330779015 1106 68-69 1.3522215689694015 1106 70-71 0.9219782363626017 1106 72-73 0.9749198574314342 1106 74-75 1.2716880357358669 1106 76-77 0.936670969505986 1106 78-79 0.7458319536476061 1106 80-81 0.9431266005600492 1106 82-83 1.4857212319964255 1106 84-85 0.9460918534580429 1106 86-87 1.1232770717605582 1106 88-89 1.3772818014542985 1106 90-91 1.6005656617000383 1106 92-93 1.5440234482577573 1106 94-95 1.6858630322303654 1106 96-97 1.6557494233574985 1106 98-99 1.9216414276575442 1106 100-101 2.074520310690904 1107 1 0.1650831566580777 1107 2 0.11856704552971209 1107 3 0.14731509394079012 1107 4 -0.024702944861033416 1107 5 0.04504337842092099 1107 6 0.030357474895993164 1107 7 0.32637739232524154 1107 8 0.17307226549820598 1107 9 0.28386587572811095 1107 10-11 0.12741203415296098 1107 12-13 0.10282232543473668 1107 14-15 -0.006784552245655107 1107 16-17 0.3753066466641286 1107 18-19 0.32016254780370446 1107 20-21 -0.13100292282415182 1107 22-23 -0.008186960802767373 1107 24-25 0.2223616960083703 1107 26-27 0.20862875825267935 1107 28-29 -0.1604061516918307 1107 30-31 -0.12378052427526143 1107 32-33 0.08932403154346957 1107 34-35 -0.05348489532749312 1107 36-37 -0.2707193001556192 1107 38-39 -0.08396924675681561 1107 40-41 -0.09807579415702605 1107 42-43 -0.1077498839951403 1107 44-45 -0.4393416828384815 1107 46-47 -0.2937865590977893 1107 48-49 -0.6160381256996104 1107 50-51 -0.1777626215943684 1107 52-53 0.1625412193042166 1107 54-55 0.7519148379027669 1107 56-57 0.5747707916180289 1107 58-59 0.4041822156210557 1107 60-61 0.4860407483102094 1107 62-63 0.6122961248845087 1107 64-65 0.5599298796993253 1107 66-67 0.55191577210838 1107 68-69 0.8681823385845853 1107 70-71 0.9056114197709029 1107 72-73 1.2226397474864044 1107 74-75 0.8860058768153287 1107 76-77 0.5658251424195271 1107 78-79 0.9617864763862407 1107 80-81 1.0007852712247143 1107 82-83 0.9022629611318571 1107 84-85 0.3347412787453976 1107 86-87 0.18924408825231254 1107 88-89 0.06746795837583974 1107 90-91 1.0860104393112344 1107 92-93 1.4643445377130284 1107 94-95 1.5878183045941832 1107 96-97 1.8315989985698913 1107 98-99 1.9783630668379537 1107 100-101 2.0048051682621164 1108 1 -0.2767531060616264 1108 2 -0.4400592264406846 1108 3 -0.30273115952821783 1108 4 -0.3933430928721364 1108 5 -0.21201027560313435 1108 6 -0.22727434749068465 1108 7 0.14402309075261854 1108 8 0.021129619799779675 1108 9 -0.16039869411462604 1108 10-11 -0.36732663374713326 1108 12-13 -0.013331698617065513 1108 14-15 -0.776326642902454 1108 16-17 -0.6026073218835108 1108 18-19 -0.16197204979111746 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-0.6264201423343891 2316 56-57 -0.6681886945479789 2316 58-59 -0.3318602744975223 2316 60-61 -0.3217655758004625 2316 62-63 0.09586825927186027 2316 64-65 0.3330277057862787 2316 66-67 -0.2813352160339093 2316 68-69 -1.0410320882928836 2316 70-71 -0.4240575525611163 2316 72-73 -0.35851143828434573 2316 74-75 -0.5566818702202418 2316 76-77 -0.43121043070300047 2316 78-79 -0.06810482796158723 2316 80-81 -0.03515840466461384 2316 82-83 -0.335138224638893 2316 84-85 0.31769582419994435 2316 86-87 -0.11633891570025412 2316 88-89 -0.260224729371199 2316 90-91 0.40604502429146905 2316 92-93 -0.1527503239470498 2316 94-95 -0.2710008653662932 2316 96-97 -0.6938456654617298 2316 98-99 -0.5994531386956403 2316 100-101 -1.0161928554532977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 1.0 5 14.0 6 37.0 7 71.0 8 103.0 9 231.0 10 345.0 11 531.0 12 785.0 13 966.0 14 1324.0 15 1849.0 16 2454.0 17 3326.0 18 2902.0 19 1747.0 20 1543.0 21 1598.0 22 1610.0 23 1794.0 24 2053.0 25 2205.0 26 2561.0 27 3025.0 28 3595.0 29 4517.0 30 5456.0 31 6536.0 32 8092.0 33 10269.0 34 13925.0 35 19108.0 36 26485.0 37 43779.0 38 38206.0 39 2971.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.65697432145867 24.959949624498794 18.642176517978683 19.740899536063857 2 14.082883516809094 25.605747775607135 40.56079698538058 19.75057172220319 3 15.260263318704517 31.016332425976334 33.824046812214135 19.89935744310501 4 10.724668543070884 22.552959040071105 42.50564772979779 24.216724687060218 5 9.726176422933593 40.77864963081267 38.01726731939912 11.477906626854617 6 23.65298706108372 43.86454644353401 19.52179246811564 12.960674027266625 7 21.74190800681431 36.77690541441375 26.808199392637583 14.672987186134359 8 21.95855862528702 37.00374046366936 23.885730686615805 17.15197022442782 9 23.882490185912157 18.99164876675802 25.443022739056364 31.68283830827346 10-11 20.071244887519647 30.359858436084448 31.55583742833269 18.013059248063218 12-13 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34.53278210386941 17.302386089017695 38-39 16.500006944155103 31.394062284441855 35.32723173571471 16.778699035688327 40-41 16.35306018050216 30.83281444943139 35.982148850623936 16.83197651944252 42-43 16.77075135410398 30.72195731679089 34.50071755937225 18.006573769732885 44-45 17.153246657053113 31.114577956572358 34.38403440509781 17.348140981276718 46-47 17.64776683208651 30.923635286275093 34.30417191319162 17.124425968446783 48-49 17.5010994606856 31.23047010624262 33.77820984653844 17.490220586533344 50-51 17.427453122576853 31.188039747147773 33.215209093791884 18.169298036483493 52-53 17.50700766387286 31.67348337025232 33.53424670217371 17.285262263701114 54-55 18.201177734157994 31.238889300396284 32.92563238398578 17.634300581459946 56-57 18.182154860333128 31.562769079781866 32.07940226096457 18.175673798920442 58-59 17.485944165526693 31.031625338231176 33.293289539774136 18.189140956467998 60-61 18.216033294292473 32.199682426520624 32.00640702179962 17.577877257387286 62-63 17.721603955262573 31.943004221909487 33.1836530627361 17.151738760091845 64-65 17.934361191673744 31.432466651698604 32.6383986334314 17.994773523196255 66-67 17.76932673812759 32.00395342000921 31.869240864660174 18.357478977203026 68-69 17.372555736612103 31.431005110732542 32.66748759351159 18.528951559143767 70-71 18.108001759137096 31.181399439853713 32.79795384579774 17.912644955211444 72-73 17.807014295237387 30.942383897489073 32.36126027701652 18.889341530257017 74-75 17.564752447746685 31.755202185033447 31.777422864152953 18.902622503066915 76-77 17.27133426537169 31.266954919588546 32.51502217448869 18.946688640551077 78-79 17.35851676966877 30.802027636969655 32.28363771034419 19.555817883017383 80-81 18.05669365703254 31.95950982162285 30.838460576936434 19.145335944408178 82-83 18.177041500105318 31.03978260416691 31.426567567004533 19.35660832872324 84-85 17.26440687381949 31.059266323469505 31.211806970112217 20.464519832598793 86-87 17.353202599855564 31.320250726811473 31.423254263652016 19.903292409680944 88-89 17.71212843671446 30.60764861374084 31.71012855707758 19.970094392467118 90-91 17.186769437307596 29.76714580006018 32.629447029141495 20.41663773349073 92-93 17.84867787571291 30.957429079327458 31.435634397452038 19.758258647507592 94-95 17.50596023424299 29.741916070643242 32.15054510103465 20.601578594079115 96-97 17.664024072680977 30.14825530929923 31.406284358544067 20.781436259475726 98-99 17.2569597955636 30.679348266387663 31.344136920537853 20.719555017510885 100-101 16.72657832301371 30.795440078699148 31.297957294138072 21.180024304149065 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2781.0 1 1744.0 2 616.5 3 475.5 4 364.0 5 334.5 6 448.0 7 746.0 8 975.5 9 958.0 10 783.5 11 606.0 12 590.0 13 626.0 14 652.0 15 670.5 16 684.5 17 697.0 18 719.5 19 749.0 20 789.0 21 825.5 22 893.5 23 1023.0 24 1138.5 25 1375.0 26 1881.0 27 2414.5 28 2526.0 29 2880.5 30 4030.5 31 4999.5 32 6477.0 33 8521.5 34 9927.0 35 11606.0 36 14133.5 37 15497.0 38 14793.0 39 13307.5 40 11556.5 41 9719.5 42 7715.0 43 7212.5 44 7493.5 45 7007.5 46 5740.0 47 4888.0 48 5131.0 49 4371.0 50 2981.0 51 1852.0 52 1254.0 53 967.0 54 726.0 55 618.0 56 550.5 57 457.5 58 411.5 59 393.0 60 346.5 61 309.5 62 216.5 63 109.0 64 44.0 65 24.5 66 13.5 67 13.5 68 9.5 69 3.5 70 3.0 71 3.0 72 2.5 73 2.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017591289534108587 2 9.258573439004517E-4 3 0.0018517146878009034 4 0.0 5 4.6292867195022586E-4 6 4.6292867195022586E-4 7 0.0 8 0.0 9 0.0 10-11 2.3146433597511293E-4 12-13 6.943930079253388E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0023146433597511294 22-23 0.0 24-25 0.005786608399377824 26-27 0.0020831790237760164 28-29 0.0032405007036515813 30-31 0.01735982519813347 32-33 9.258573439004517E-4 34-35 0.0027775720317013554 36-37 0.006481001407303163 38-39 0.0032405007036515813 40-41 0.0034719650396266943 42-43 0.0027775720317013554 44-45 6.943930079253388E-4 46-47 0.0 48-49 4.6292867195022586E-4 50-51 2.3146433597511293E-4 52-53 0.0011573216798755647 54-55 0.003703429375601807 56-57 9.258573439004517E-4 58-59 0.0011573216798755647 60-61 0.0013887860158506777 62-63 0.0 64-65 2.3146433597511293E-4 66-67 2.3146433597511293E-4 68-69 0.0 70-71 4.6292867195022586E-4 72-73 0.0 74-75 4.6292867195022586E-4 76-77 9.258573439004517E-4 78-79 4.6292867195022586E-4 80-81 0.006018072735352937 82-83 0.0020831790237760164 84-85 0.003703429375601807 86-87 0.0018517146878009034 88-89 0.0013887860158506777 90-91 4.6292867195022586E-4 92-93 0.0 94-95 4.6292867195022586E-4 96-97 0.0016202503518257907 98-99 0.0034719650396266943 100-101 0.0016202503518257907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 216016.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.29844085623287 #Duplication Level Percentage of deduplicated Percentage of total 1 74.52051077684885 20.342937560180726 2 9.474469636588715 5.1727649803718245 3 3.6595499330156525 2.9970002222057626 4 2.0892333259848397 2.281312495370713 5 1.4363479116145772 1.9605029257092066 6 1.014092150112771 1.6609880749574104 7 0.741067340467025 1.416098807495741 8 0.6342315453882548 1.385082586475076 9 0.5341789753938511 1.3124027849788904 >10 4.848310129050857 27.574346344715206 >50 0.6952805711475522 13.24114880379231 >100 0.34085706048940967 14.865102584993704 >500 0.0033916125421831807 0.49950003703429374 >1k 0.008479031355457953 5.290811791719132 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 1.3480482927190578 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2830 1.3100881416191394 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2265 1.0485334419672616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2078 0.9619657803125694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1344 0.6221761351011036 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 561 0.25970298496407673 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 518 0.23979705207021704 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 491 0.22729797792756093 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 437 0.2022998296422487 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 347 0.1606362491667284 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 341 0.15785867713502705 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 336 0.1555440337752759 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 326 0.15091474705577365 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 322 0.14906303236797275 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 314 0.14535960299237094 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 306 0.14165617361676913 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 290 0.13424931486556552 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 278 0.1286941708021628 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 276 0.12776831345826234 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 274 0.1268424561143619 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 273 0.12637952744241168 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 272 0.12591659877046146 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 265 0.12267609806680987 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 261 0.12082438337900896 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 257 0.11897266869120805 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 249 0.11526923931560625 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 242 0.11202873861195467 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 241 0.11156580994000445 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 223 0.10323309384490037 No Hit ATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTC 223 0.10323309384490037 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 222 0.10277016517295015 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 221 0.10230723650099993 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 220 0.10184430782904971 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 219 0.10138137915709947 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 218 0.10091845048514925 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 218 0.10091845048514925 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.10045552181319901 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.629286719502259E-4 7 0.0 0.0 0.0 0.0 4.629286719502259E-4 8 0.0 0.0 0.0 0.0 4.629286719502259E-4 9 0.0 0.0 0.0 0.0 4.629286719502259E-4 10-11 0.0 0.0 0.0 0.0 4.629286719502259E-4 12-13 0.0 0.0 0.0 0.0 4.629286719502259E-4 14-15 0.0 0.0 0.0 0.0 4.629286719502259E-4 16-17 0.0 0.0 0.0 2.3146433597511296E-4 4.629286719502259E-4 18-19 0.0 0.0 0.0 6.943930079253388E-4 4.629286719502259E-4 20-21 0.0 0.0 0.0 0.0013887860158506777 4.629286719502259E-4 22-23 0.0 0.0 0.0 0.0030090363676764684 4.629286719502259E-4 24-25 0.0 0.0 0.0 0.006943930079253389 4.629286719502259E-4 26-27 0.0 0.0 0.0 0.01018443078290497 4.629286719502259E-4 28-29 0.0 0.0 0.0 0.017591289534108583 4.629286719502259E-4 30-31 0.0 0.0 0.0 0.028470113324938894 4.629286719502259E-4 32-33 0.0 0.0 0.0 0.043515295163321234 4.629286719502259E-4 34-35 0.0 0.0 0.0 0.061106584697429814 4.629286719502259E-4 36-37 0.0 0.0 0.0 0.07846640989556329 4.629286719502259E-4 38-39 0.0 0.0 0.0 0.1002240574772239 4.629286719502259E-4 40-41 0.0 0.0 0.0 0.12059291904303385 4.629286719502259E-4 42-43 0.0 0.0 0.0 0.1752185023331605 4.629286719502259E-4 44-45 0.0 0.0 0.0 0.23007554995926227 4.629286719502259E-4 46-47 0.0 0.0 0.0 0.27590548848233465 4.629286719502259E-4 48-49 0.0 0.0 0.0 0.3427986815791423 4.629286719502259E-4 50-51 0.0 0.0 0.0 0.4286719502259092 4.629286719502259E-4 52-53 0.0 0.0 0.0 0.5687078734908526 4.629286719502259E-4 54-55 0.0 0.0 0.0 0.704808903044219 4.629286719502259E-4 56-57 0.0 0.0 0.0 0.9124324124138953 4.629286719502259E-4 58-59 0.0 0.0 0.0 1.1834771498407526 4.629286719502259E-4 60-61 0.0 0.0 0.0 1.430218131990223 4.629286719502259E-4 62-63 0.0 0.0 0.0 1.7362139841493223 4.629286719502259E-4 64-65 0.0 0.0 0.0 1.9908247537219466 4.629286719502259E-4 66-67 0.0 0.0 0.0 2.373203836752833 4.629286719502259E-4 68-69 0.0 0.0 0.0 2.803496037330568 4.629286719502259E-4 70-71 0.0 0.0 0.0 3.1812458336419525 4.629286719502259E-4 72-73 0.0 0.0 0.0 3.684680764387823 4.629286719502259E-4 74-75 0.0 0.0 0.0 4.1827920154062666 4.629286719502259E-4 76-77 0.0 0.0 0.0 4.5797533516035855 4.629286719502259E-4 78-79 0.0 0.0 0.0 4.997083549366714 4.629286719502259E-4 80-81 0.0 0.0 0.0 5.595418857862381 4.629286719502259E-4 82-83 0.0 0.0 0.0 6.120611436189912 4.629286719502259E-4 84-85 0.0 0.0 0.0 6.9094418931930965 4.629286719502259E-4 86-87 0.0 0.0 0.0 7.802199837049107 4.629286719502259E-4 88-89 0.0 0.0 0.0 8.808838234204874 4.629286719502259E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACTGGT 35 2.7849092E-4 54.27764 7 AGCTCCG 25 0.0016140421 38.00315 46-47 GATCCTC 50 0.00161409 37.99435 5 TGGACTG 65 1.3257755E-4 36.533028 5 GGGTCCT 65 1.3257755E-4 36.533028 7 TGGGTAT 65 1.3257755E-4 36.533028 6 GGACTGG 65 1.3257755E-4 36.533028 6 GGGGTCC 65 1.3257755E-4 36.533028 6 AGGGGTC 70 2.049625E-4 33.923527 5 CCTCATC 90 2.4064117E-5 31.66196 8 GGTTACT 60 0.003946785 31.66196 8 GGTCCTA 75 3.072917E-4 31.661959 8 ATGGGTA 95 3.481402E-5 29.99554 5 CGGCTCG 40 4.480789E-4 29.696835 36-37 GTAGATA 65 0.0058379485 29.226423 6 ACTGGTA 65 0.0058379485 29.226423 8 CATGGGT 115 3.8499456E-6 28.908747 4 CCCTATA 100 4.9397633E-5 28.495764 2 CGCAGAA 100 4.9397633E-5 28.495764 9 ACCCTTG 170 3.612513E-9 27.937023 7 >>END_MODULE