##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937118_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156830 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90200216795256 33.0 31.0 34.0 30.0 34.0 2 32.022999426130205 33.0 31.0 34.0 30.0 34.0 3 31.980201492061468 33.0 31.0 34.0 30.0 34.0 4 35.60236561882293 37.0 35.0 37.0 33.0 37.0 5 35.63325894280431 37.0 35.0 37.0 33.0 37.0 6 35.70306063890837 37.0 35.0 37.0 35.0 37.0 7 35.66772301217879 37.0 35.0 37.0 33.0 37.0 8 35.63082318433973 37.0 35.0 37.0 33.0 37.0 9 37.286290888222915 39.0 37.0 39.0 34.0 39.0 10-11 37.143869157686666 39.0 37.0 39.0 34.0 39.0 12-13 37.12237454568641 39.0 37.0 39.0 33.5 39.0 14-15 38.272648727921954 40.0 38.0 41.0 33.5 41.0 16-17 38.196744882994324 40.0 38.0 41.0 33.0 41.0 18-19 38.173653000063766 40.0 38.0 41.0 33.5 41.0 20-21 38.12620990881847 40.0 38.0 41.0 34.0 41.0 22-23 38.01752853408149 40.0 37.5 41.0 33.5 41.0 24-25 37.89046738506663 40.0 37.0 41.0 33.0 41.0 26-27 37.89189568322387 40.0 37.0 41.0 33.0 41.0 28-29 37.818060957724924 40.0 37.0 41.0 33.0 41.0 30-31 37.6299560033157 40.0 37.0 41.0 33.0 41.0 32-33 37.350153031945425 40.0 36.0 41.0 33.0 41.0 34-35 37.18117069438246 39.5 35.0 41.0 33.0 41.0 36-37 36.98945036026271 39.0 35.0 41.0 32.0 41.0 38-39 36.81568577440541 39.0 35.0 41.0 31.5 41.0 40-41 36.65641777721099 39.0 35.0 41.0 31.0 41.0 42-43 36.40941465280878 39.0 35.0 41.0 31.0 41.0 44-45 36.10012433845566 39.0 35.0 41.0 30.0 41.0 46-47 35.663600714149084 39.0 35.0 41.0 29.0 41.0 48-49 35.27598354906587 39.0 35.0 41.0 27.0 41.0 50-51 34.52010138366384 38.5 34.0 40.0 24.5 40.5 52-53 34.731244022189635 38.5 35.0 40.0 24.5 40.5 54-55 35.10750494165657 39.0 35.0 41.0 24.5 41.0 56-57 35.00715743161385 39.0 35.0 41.0 23.5 41.0 58-59 34.791481221705034 39.0 35.0 41.0 22.5 41.0 60-61 34.560218070522225 39.0 35.0 41.0 21.5 41.0 62-63 34.2478639290952 39.0 35.0 40.5 19.0 41.0 64-65 33.94747178473506 38.0 34.5 40.0 14.0 41.0 66-67 33.583000701396415 38.0 34.0 40.0 8.0 41.0 68-69 33.1971848498374 37.0 34.0 40.0 4.0 41.0 70-71 32.75772492507811 37.0 33.5 39.0 2.0 41.0 72-73 32.32615889816999 36.0 33.0 39.0 2.0 41.0 74-75 31.847541924376713 36.0 33.0 39.0 2.0 40.0 76-77 31.419205509150036 35.0 32.0 37.5 2.0 40.0 78-79 30.926302365618824 35.0 31.5 37.0 2.0 39.0 80-81 30.44958235031563 35.0 31.0 37.0 2.0 39.0 82-83 29.998651405981 35.0 31.0 36.0 2.0 38.5 84-85 29.567123637059236 35.0 30.5 36.0 2.0 37.0 86-87 29.219039724542498 35.0 30.0 35.0 2.0 37.0 88-89 28.8234521456354 34.0 30.0 35.0 2.0 36.5 90-91 28.440218708155328 34.0 29.0 35.0 2.0 36.0 92-93 28.06375055792897 34.0 29.0 35.0 2.0 36.0 94-95 27.669154498501563 34.0 29.0 35.0 2.0 35.0 96-97 27.231712682522478 34.0 27.5 35.0 2.0 35.0 98-99 26.732283364152266 34.0 25.5 35.0 2.0 35.0 100-101 24.778572339475865 31.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35524257261293357 1101 2 0.42882555474391637 1101 3 0.521460412789299 1101 4 0.3364687837679128 1101 5 0.5245128539035093 1101 6 0.3987759525462451 1101 7 0.2414178955105868 1101 8 0.24843844723304187 1101 9 0.3006932255104289 1101 10-11 0.30042302375498764 1101 12-13 0.38216533053052615 1101 14-15 0.5910230227493685 1101 16-17 0.7050408792168952 1101 18-19 0.510289143773079 1101 20-21 0.48140241324546196 1101 22-23 0.6649410004367624 1101 24-25 0.5540208642317097 1101 26-27 0.5766451286253798 1101 28-29 0.6754420602719975 1101 30-31 0.8297621261649937 1101 32-33 0.9633139612723554 1101 34-35 0.6710003610225073 1101 36-37 0.6316419668459687 1101 38-39 1.1083499140845134 1101 40-41 1.2118848669765612 1101 42-43 1.1509147569872837 1101 44-45 1.3032537665626975 1101 46-47 1.0609981335821317 1101 48-49 1.360689726859185 1101 50-51 1.261293927418798 1101 52-53 1.319442285434036 1101 54-55 1.5837853728712403 1101 56-57 1.987679176412577 1101 58-59 1.5396686919456073 1101 60-61 1.6154094971776232 1101 62-63 1.73164655275162 1101 64-65 1.6597408576379067 1101 66-67 1.875252222353474 1101 68-69 1.8337346085954636 1101 70-71 1.7842426760949799 1101 72-73 1.7167136294468328 1101 74-75 1.948970756200719 1101 76-77 2.164658146624344 1101 78-79 1.8713108722810006 1101 80-81 1.8294667419648718 1101 82-83 1.642406174678765 1101 84-85 1.664352147754709 1101 86-87 1.8286827130240049 1101 88-89 2.179885231624393 1101 90-91 2.324862429601886 1101 92-93 2.780020247705753 1101 94-95 2.8359107020729155 1101 96-97 2.542878211229514 1101 98-99 2.4642669275293834 1101 100-101 2.41043366339364 1102 1 0.3466352620146047 1102 2 0.26023253861158935 1102 3 0.34685351060428715 1102 4 0.39404485074769724 1102 5 0.3196425251307886 1102 6 0.4469993546291491 1102 7 0.3987999661660879 1102 8 0.3929861294874897 1102 9 0.3193577139727495 1102 10-11 0.2385943531403285 1102 12-13 0.4165843625909673 1102 14-15 0.5892157938337093 1102 16-17 0.6153124740708833 1102 18-19 0.36171859037303733 1102 20-21 0.5384526480831227 1102 22-23 0.8116583784232816 1102 24-25 0.6350960122815366 1102 26-27 0.42383728708973933 1102 28-29 0.36897891957361395 1102 30-31 0.5110465860383897 1102 32-33 0.5303907429754346 1102 34-35 0.4471183924703297 1102 36-37 0.6138964083407572 1102 38-39 1.0726341697512467 1102 40-41 0.6994844177219122 1102 42-43 0.9357575178612834 1102 44-45 0.8294432703405192 1102 46-47 1.4532025444459222 1102 48-49 1.713666038886963 1102 50-51 1.4960243746313822 1102 52-53 1.157480880492237 1102 54-55 1.4250504331122045 1102 56-57 1.9595599197248106 1102 58-59 1.8768445138774084 1102 60-61 1.8607893215598992 1102 62-63 1.8415250497906186 1102 64-65 2.3397388155713443 1102 66-67 2.4625199372809803 1102 68-69 2.0982281760857546 1102 70-71 2.235192237050665 1102 72-73 2.22341160780093 1102 74-75 2.350854562612806 1102 76-77 2.5297388082539065 1102 78-79 2.6741238189830625 1102 80-81 2.688395678048181 1102 82-83 2.8148178552995518 1102 84-85 2.7652353415468234 1102 86-87 2.448521389764867 1102 88-89 2.699330810550265 1102 90-91 2.6449563646641714 1102 92-93 2.7619530637155556 1102 94-95 2.619992793149084 1102 96-97 2.633479599834782 1102 98-99 2.502096210764016 1102 100-101 2.8872511026421606 1103 1 0.044773423288358316 1103 2 0.3273742951601264 1103 3 0.2505129867213931 1103 4 0.2598897403213982 1103 5 0.11053877577280247 1103 6 0.3656434224257623 1103 7 0.3389900021188339 1103 8 0.35691781926663424 1103 9 0.3601127216769129 1103 10-11 0.5445586571978538 1103 12-13 0.4731200585334463 1103 14-15 0.6278521574700662 1103 16-17 0.515395935806886 1103 18-19 0.38556296633606024 1103 20-21 0.45431679805743386 1103 22-23 0.3367418401592843 1103 24-25 0.3790482464880043 1103 26-27 0.40646440573380715 1103 28-29 0.27636849327674184 1103 30-31 0.5005817683701821 1103 32-33 0.6888524789795483 1103 34-35 0.5189147458344792 1103 36-37 0.4942058587773275 1103 38-39 0.7840299067825782 1103 40-41 0.917274607757868 1103 42-43 1.0392179698386883 1103 44-45 1.1773182061391836 1103 46-47 1.4079085023298532 1103 48-49 1.9074449294878875 1103 50-51 2.000820214384852 1103 52-53 2.1565178604614204 1103 54-55 2.2333837664455345 1103 56-57 1.9189268945578846 1103 58-59 2.1162705539390387 1103 60-61 2.472884853146958 1103 62-63 2.3034793384398213 1103 64-65 2.5416776959000558 1103 66-67 2.5241660595203186 1103 68-69 2.580585649121197 1103 70-71 2.3957340963213376 1103 72-73 2.4866114023566546 1103 74-75 2.5232223078619107 1103 76-77 2.428531823148621 1103 78-79 2.516573741941457 1103 80-81 2.5398081074267154 1103 82-83 2.556060793152657 1103 84-85 2.2664423266521148 1103 86-87 2.3312383902784752 1103 88-89 2.686342880401316 1103 90-91 2.6932678695434724 1103 92-93 2.727560408348321 1103 94-95 2.635837682722787 1103 96-97 2.7139944945445933 1103 98-99 2.554035091092725 1103 100-101 2.1400567009986062 1104 1 0.34158078259571667 1104 2 0.4182833860692199 1104 3 0.5268928568512656 1104 4 0.29790380958546336 1104 5 0.6204143168983407 1104 6 0.6335005652829082 1104 7 0.45005601943485374 1104 8 0.5993420616908764 1104 9 0.6437977866119766 1104 10-11 0.6519720771112674 1104 12-13 0.7474030671914562 1104 14-15 0.755287222405137 1104 16-17 0.9317243990103563 1104 18-19 0.7017155637386523 1104 20-21 0.9125824906981279 1104 22-23 0.543248874791324 1104 24-25 0.658877791942551 1104 26-27 0.8149465485909531 1104 28-29 0.9422234716317277 1104 30-31 0.9391640194524342 1104 32-33 0.80526211101418 1104 34-35 0.9794125813323191 1104 36-37 1.1992247981712651 1104 38-39 0.9358832617609281 1104 40-41 0.9966847809180379 1104 42-43 1.3174917793624914 1104 44-45 1.2790498078707842 1104 46-47 0.8721265759229269 1104 48-49 0.9496527216956778 1104 50-51 1.0274030065926425 1104 52-53 0.8537581202016753 1104 54-55 0.8978182902550671 1104 56-57 1.3206449681509582 1104 58-59 1.433843605887084 1104 60-61 1.390904333666434 1104 62-63 1.5912904856259686 1104 64-65 1.5441804015277611 1104 66-67 1.2888440898233497 1104 68-69 1.295277207562762 1104 70-71 1.198426195888441 1104 72-73 1.1344739564692112 1104 74-75 1.2666611606757634 1104 76-77 1.254965805832601 1104 78-79 1.6618659497336665 1104 80-81 1.3820700121886205 1104 82-83 1.2219834122002773 1104 84-85 1.3350976296824193 1104 86-87 1.2909245374646225 1104 88-89 1.4558910405744783 1104 90-91 1.6004648392404413 1104 92-93 1.4381258845388025 1104 94-95 1.2296553234154288 1104 96-97 1.2484236070987045 1104 98-99 1.4918056538632918 1104 100-101 1.085132458574364 1105 1 0.11674312025805733 1105 2 0.12282884061467314 1105 3 0.3186948049032132 1105 4 0.040192770624429386 1105 5 0.47228120001522456 1105 6 0.3940525133348558 1105 7 0.1590657596945917 1105 8 0.28116024350905633 1105 9 -0.06034182377763386 1105 10-11 0.1515662329554317 1105 12-13 0.23827157368495477 1105 14-15 0.40626124837915967 1105 16-17 0.05043381459476848 1105 18-19 0.04465387542696675 1105 20-21 0.10677891926955851 1105 22-23 0.13882517349262002 1105 24-25 0.18018461012436404 1105 26-27 0.18771440573380715 1105 28-29 0.02826243267068662 1105 30-31 0.08202116230957301 1105 32-33 -0.19372327859620952 1105 34-35 -0.2661231329533962 1105 36-37 -0.4186729291014615 1105 38-39 -0.2453261538234841 1105 40-41 -0.10639963466636715 1105 42-43 0.2342937274144461 1105 44-45 0.500234872805855 1105 46-47 0.9287418356631818 1105 48-49 1.4131267476697005 1105 50-51 0.8786611234757586 1105 52-53 1.1565178604614204 1105 54-55 1.2665277058394793 1105 56-57 1.4568056824366735 1105 58-59 1.2412705539390387 1105 60-61 1.3933393986015048 1105 62-63 1.5781005505610395 1105 64-65 1.1174352716576266 1105 66-67 1.196514544368803 1105 68-69 1.4839947400302833 1105 70-71 1.5955447023819396 1105 72-73 1.8881265538718033 1105 74-75 2.1378056411952393 1105 76-77 2.1311833383001293 1105 78-79 1.6927101055778238 1105 80-81 1.6685959862145978 1105 82-83 1.792803217395086 1105 84-85 1.768336266046056 1105 86-87 1.4808596023996863 1105 88-89 1.7677822743407106 1105 90-91 1.1544421119677146 1105 92-93 1.33646192349984 1105 94-95 1.7257998039349047 1105 96-97 1.4677823733324686 1105 98-99 1.0275199395775765 1105 100-101 1.1447915494834575 1106 1 0.23362623714116992 1106 2 0.15367299645883037 1106 3 0.2326558438642472 1106 4 0.19279017322183023 1106 5 0.26692405715808576 1106 6 0.2227862795686164 1106 7 0.21669562982446422 1106 8 0.344471931820749 1106 9 0.03868415024833638 1106 10-11 0.12437467451386652 1106 12-13 0.4476871581005426 1106 14-15 0.11648852110643304 1106 16-17 0.2521383600493081 1106 18-19 0.49108244685553615 1106 20-21 0.5584834647241053 1106 22-23 0.4712115371289869 1106 24-25 0.6339183763581318 1106 26-27 0.414581288850691 1106 28-29 0.25391178332003506 1106 30-31 0.2532873960758053 1106 32-33 0.6374455525726219 1106 34-35 0.4899645293842667 1106 36-37 0.4863595384310031 1106 38-39 0.6706641059167779 1106 40-41 0.5863763393596031 1106 42-43 0.43883918195989935 1106 44-45 0.14674461306559294 1106 46-47 -0.11184257992123747 1106 48-49 0.06247739702035204 1106 50-51 0.3766319026965377 1106 52-53 0.219829548773113 1106 54-55 0.3659595240212994 1106 56-57 0.5956043837353704 1106 58-59 0.3411082162767016 1106 60-61 0.3560017362638419 1106 62-63 0.11137977134026045 1106 64-65 0.16126644048879513 1106 66-67 0.4944041547584135 1106 68-69 0.37604019457574367 1106 70-71 0.4222492478364863 1106 72-73 0.8037109694562226 1106 74-75 0.7591530437926437 1106 76-77 1.2164106110274062 1106 78-79 1.2665737419414604 1106 80-81 1.2724920901107026 1106 82-83 1.0712123083041725 1106 84-85 0.9720700322798201 1106 86-87 1.397255706295791 1106 88-89 1.3895355210939577 1106 90-91 1.2481921119677146 1106 92-93 1.2451469884349038 1106 94-95 1.2083485052336087 1106 96-97 1.369568087618184 1106 98-99 1.4528446149022507 1106 100-101 1.4731194715613753 1107 1 -0.09304708953215268 1107 2 0.399926742712573 1107 3 0.17271578392418618 1107 4 -0.22029673986508413 1107 5 0.3293616195956446 1107 6 0.28172733850967546 1107 7 0.4759363890652253 1107 8 0.6002161875650032 1107 9 0.30591691748110605 1107 10-11 0.26673231687151144 1107 12-13 0.26187297228635487 1107 14-15 0.5185864232043329 1107 16-17 0.5108796187905753 1107 18-19 0.5385349943080868 1107 20-21 0.6668750731157118 1107 22-23 0.29114160705905334 1107 24-25 0.6561461485859112 1107 26-27 0.8793665036359073 1107 28-29 0.523891803300053 1107 30-31 0.6104302532186665 1107 32-33 0.5318011969282637 1107 34-35 0.7804240698438036 1107 36-37 0.5922536443251118 1107 38-39 0.25882594407861603 1107 40-41 0.9707419737252323 1107 42-43 1.225552468673186 1107 44-45 0.9624289287499082 1107 46-47 1.758287290208635 1107 48-49 1.9638260483690004 1107 50-51 1.891117417182052 1107 52-53 1.8829164618600203 1107 54-55 1.8467287547905258 1107 56-57 2.5988511369821268 1107 58-59 2.865833491001972 1107 60-61 2.2928149230770245 1107 62-63 1.9832578932183793 1107 64-65 2.1882394674618197 1107 66-67 2.371776782131043 1107 68-69 2.5228933414288903 1107 70-71 2.0411303667176064 1107 72-73 2.174839840585097 1107 74-75 2.2341780188176195 1107 76-77 2.3475294921462897 1107 78-79 2.8365038118715304 1107 80-81 2.733281300899911 1107 82-83 2.8858976229894857 1107 84-85 2.815976126185916 1107 86-87 2.6150379240780133 1107 88-89 2.4434815750400105 1107 90-91 3.0139263777019814 1107 92-93 2.3917503850383 1107 94-95 2.9266302235153248 1107 96-97 2.8738138418639423 1107 98-99 3.1568905689482065 1107 100-101 3.5720205704624775 1108 1 0.09654110005603656 1108 2 0.059156401942239256 1108 3 0.2377063489147524 1108 4 -0.09941761898596013 1108 5 0.04253733277136007 1108 6 0.21484977163211028 1108 7 -0.29051937739054523 1108 8 0.037834125182939715 1108 9 -0.06304745148326418 1108 10-11 -0.04445649431730203 1108 12-13 -0.13925367884029072 1108 14-15 0.13524753986545335 1108 16-17 0.017289875200830807 1108 18-19 -0.0825106267375375 1108 20-21 0.12414003038067278 1108 22-23 -0.15938009346264437 1108 24-25 -0.029862287422524503 1108 26-27 0.1465437708131745 1108 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2313 36-37 0.8134074904789586 2313 38-39 0.01057419582686947 2313 40-41 -0.01396082347756078 2313 42-43 -0.5344999788792606 2313 44-45 -0.30680184048086545 2313 46-47 0.08040267482402186 2313 48-49 0.968196677739634 2313 50-51 -0.1066972681326348 2313 52-53 0.3558185597621275 2313 54-55 0.020242740804512493 2313 56-57 -0.2547327791017864 2313 58-59 -0.25829238312390146 2313 60-61 -0.05071654545444204 2313 62-63 -0.5198015473410607 2313 64-65 -0.5377220709997133 2313 66-67 -0.25322321786895685 2313 68-69 0.0888898449253901 2313 70-71 -0.10703396894672679 2313 72-73 -0.5471881314428835 2313 74-75 0.0772724244120262 2313 76-77 -0.5091138644970705 2313 78-79 -0.2941780063102897 2313 80-81 -0.21820471308610223 2313 82-83 0.11535566494753269 2313 84-85 0.2569726296824193 2313 86-87 0.0752651968052831 2313 88-89 0.22975779881623382 2313 90-91 -0.0437659299903288 2313 92-93 0.6185860493739632 2313 94-95 0.6844973563824581 2313 96-97 0.3567683873184855 2313 98-99 1.0034814780391166 2313 100-101 0.9505170739589772 2314 1 -0.3231170196020834 2314 2 -0.3105627677769327 2314 3 -0.39721428600588027 2314 4 -0.8279890475573879 2314 5 0.09369728393131282 2314 6 -0.6805104237280872 2314 7 -0.5772104640816309 2314 8 -0.6410425536937367 2314 9 -0.6381390265748408 2314 10-11 -0.6713795712403794 2314 12-13 -0.12728786687448235 2314 14-15 -0.11847651385860303 2314 16-17 -0.6083511504401926 2314 18-19 -0.8387377329646384 2314 20-21 -0.2023556961150632 2314 22-23 0.149533215450667 2314 24-25 0.007195099634863311 2314 26-27 -0.28007405580465417 2314 28-29 -0.21491938551113066 2314 30-31 -0.1343249915365874 2314 32-33 -0.3332337681067017 2314 34-35 -0.4300654406457056 2314 36-37 0.4307151827866491 2314 38-39 0.1298049650576374 2314 40-41 -0.09184543886217966 2314 42-43 -0.8643076711869568 2314 44-45 -0.9414172250962451 2314 46-47 -1.5224819405605956 2314 48-49 -0.9914187068757556 2314 50-51 -0.6634280373634098 2314 52-53 -0.8114891325455744 2314 54-55 -1.2634111053493342 2314 56-57 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78-79 1.9265594255491862 2315 80-81 2.0030627006025696 2315 82-83 2.0901815280607963 2315 84-85 1.7171104249580118 2315 86-87 2.37353139910692 2315 88-89 2.307778695242643 2315 90-91 2.050267988846741 2315 92-93 1.8428819306580344 2315 94-95 1.8853604696471464 2315 96-97 1.3859552437691214 2315 98-99 1.2322443490263986 2315 100-101 1.3971403325901122 2316 1 -1.6124916737249393 2316 2 -0.8775799232832995 2316 3 -0.702969681689332 2316 4 -0.031586169859551205 2316 5 -0.4728218084870619 2316 6 -0.9868745631857507 2316 7 -0.874166745210573 2316 8 -2.324219089388258 2316 9 -1.3182718433982998 2316 10-11 -2.2207154871230586 2316 12-13 -1.500364789951405 2316 14-15 -1.9453165802670114 2316 16-17 -1.4329499329526456 2316 18-19 -1.1931649604134478 2316 20-21 -1.2315753972772043 2316 22-23 -1.2042299278808244 2316 24-25 -1.9415044023020513 2316 26-27 -2.3983917093741027 2316 28-29 -2.4275093135686845 2316 30-31 -1.7142917873307155 2316 32-33 -1.4025198776805752 2316 34-35 -0.872013420723178 2316 36-37 -1.5236843744906068 2316 38-39 -1.2210251400890186 2316 40-41 -0.947656174888742 2316 42-43 -1.5022988167320577 2316 44-45 -0.7910298426944777 2316 46-47 -0.8820004795755381 2316 48-49 -0.8381812968038105 2316 50-51 0.07353931183416051 2316 52-53 -0.5579196804149689 2316 54-55 -0.05048913523311427 2316 56-57 -0.3916655405849099 2316 58-59 -0.5686133571139393 2316 60-61 -0.42917531689882793 2316 62-63 0.2128700077225787 2316 64-65 -0.23124033331293958 2316 66-67 -0.2306028526226953 2316 68-69 -0.10172304937455579 2316 70-71 -0.35374813607456446 2316 72-73 0.20696239429037888 2316 74-75 0.5373927896582877 2316 76-77 -0.17845531441405882 2316 78-79 -0.6726021900402266 2316 80-81 -1.1988010052831157 2316 82-83 0.16637254375217836 2316 84-85 -0.48628276600103604 2316 86-87 -1.2063133864819342 2316 88-89 -0.9337383273597446 2316 90-91 -0.5605063837811421 2316 92-93 -0.4134767619154651 2316 94-95 -1.2185878677459314 2316 96-97 -0.8023696868375758 2316 98-99 -0.7170843769691864 2316 100-101 -0.8708027932241968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 487.0 3 126.0 4 112.0 5 99.0 6 120.0 7 129.0 8 172.0 9 179.0 10 263.0 11 404.0 12 518.0 13 771.0 14 947.0 15 1205.0 16 1602.0 17 2325.0 18 2046.0 19 1118.0 20 1135.0 21 1056.0 22 1097.0 23 1224.0 24 1361.0 25 1502.0 26 1524.0 27 1747.0 28 2013.0 29 2301.0 30 2902.0 31 3454.0 32 4492.0 33 5764.0 34 7880.0 35 12333.0 36 16978.0 37 29321.0 38 37474.0 39 8647.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.409971804391375 24.77832638011763 18.054758168433676 20.756943647057323 2 13.340392417013241 24.881994233664177 41.34220396499375 20.435409384328835 3 14.495360183679326 31.478044580503205 33.53550814758124 20.49108708823623 4 9.849900805674809 22.05494919081671 43.181107786914005 24.914042216594474 5 9.27802164148632 40.39084830543079 38.318573907717436 12.012556145365455 6 22.36819437620831 44.341434468853485 20.06801635965724 13.222354795280966 7 20.926092532685058 36.926296714585604 27.149812088844648 14.997798663884687 8 20.917797642975184 36.77252222073339 24.213421131550568 18.096259004740848 9 23.165759569187188 19.151773465963107 24.94975339284233 32.73271357200738 10-11 19.353871284727983 30.445449788792605 31.890082823925773 18.31059610255363 12-13 21.337280900453674 29.895483055876365 30.876206762335617 17.891029281334344 14-15 19.42554148601181 31.793340415960795 29.47840596216856 19.302712135858833 16-17 15.904458415870007 34.254235287738 31.237938481069992 18.603367815322 18-19 15.904132484466501 32.57632784291711 34.631725334592176 16.887814338024214 20-21 16.657256328053975 31.836611002121312 34.60293155972375 16.903201110100962 22-23 17.364904727736587 30.963246432272978 34.54644024555529 17.125408594435143 24-25 17.07354404224382 31.95741180071937 34.11009923216245 16.85894492487437 26-27 15.972246579448313 32.24645190501851 34.7647942248397 17.016507290693486 28-29 16.140007397676108 31.007104319987754 35.65903090442968 17.193857377906458 30-31 16.70492806856443 31.59658453219059 34.600423426181 17.098063973063972 32-33 15.896938837325717 32.58300147621947 34.68446207263765 16.83559761381716 34-35 16.53557115027149 31.845529414390427 34.52918801552103 17.08971141981705 36-37 16.72474645224439 31.34204158151309 34.02625227563295 17.90695969060957 38-39 15.855874813482801 32.49193352973435 34.501855606993914 17.150336049788933 40-41 15.701728376168111 31.984364588215414 35.36268480170383 16.951222233912645 42-43 16.094105194022617 31.936017650303366 33.5416341196688 18.428243036005217 44-45 16.17440763129033 31.993330272655395 33.65438569643176 18.177876399622516 46-47 16.725595156492602 31.56887938097986 34.0553534593521 17.65017200317544 48-49 16.748177951781877 31.834673434122518 33.45937294760535 17.957775666490253 50-51 16.549177897445215 31.67828801168192 33.021517820989835 18.75101626988302 52-53 16.420746559881653 32.32946067616339 33.2687181972377 17.981074566717254 54-55 17.337907463143395 31.542684793143906 32.83330357598327 18.286104167729427 56-57 17.275608729480492 32.24161681611204 31.885116261272177 18.597658193135292 58-59 16.56601920697879 31.167659324822406 33.32812559782678 18.938195870372024 60-61 17.499800711062928 32.412033863176184 32.13558024966918 17.952585176091702 62-63 16.72815159409107 32.35882114741397 32.93211362542128 17.980913633073683 64-65 17.204338787639173 31.31687688913263 33.05232817661238 18.42645614661582 66-67 16.851181062190705 31.865083414111524 32.24070200499975 19.043033518698024 68-69 16.742616787262065 31.471412672243076 32.96798614720824 18.817984393286626 70-71 17.659325487295092 31.057748414477437 32.88831352692279 18.394612571304673 72-73 17.00773542672389 30.829788726556174 32.75376089687585 19.40871494984408 74-75 16.84980516954395 31.5982474825099 32.14479314809028 19.40715419985587 76-77 16.609958704698734 31.028555939986287 33.114048374495766 19.24743698081921 78-79 16.515019597712698 30.604323934723194 32.68273392078633 20.19792254677778 80-81 17.125563655613597 31.554190663885862 31.68398293247613 19.636262748024418 82-83 17.5747428844774 30.834409630869807 31.83066252092801 19.760184963724786 84-85 16.847895408163264 30.279017857142858 31.849808673469386 21.02327806122449 86-87 17.230449367976632 30.936300207910815 32.00232145818187 19.83092896593069 88-89 17.729046823382355 29.607721840282647 32.25947373120241 20.40375760513259 90-91 17.547344891471063 29.145767100442278 32.79751788088762 20.509370127199038 92-93 18.24765841011069 30.0932171228661 31.80512104193973 19.854003425083473 94-95 17.6546556122449 29.11352040816326 32.51753826530612 20.714285714285715 96-97 17.859102101220927 29.5102254315349 31.924652029138972 20.706020438105202 98-99 17.99332682603176 30.46087006449884 31.38656561210103 20.15923749736837 100-101 18.041824185289755 29.871273388524667 31.215006938795042 20.871895487390535 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1782.0 1 1128.0 2 431.0 3 362.0 4 269.5 5 236.5 6 388.5 7 656.5 8 857.0 9 886.5 10 737.5 11 556.5 12 524.0 13 529.0 14 517.0 15 515.0 16 493.5 17 504.5 18 506.0 19 495.0 20 564.0 21 626.5 22 681.5 23 762.0 24 839.5 25 978.0 26 1329.0 27 1715.5 28 1820.0 29 2117.0 30 2955.0 31 3729.5 32 4886.5 33 6451.0 34 7421.5 35 8336.5 36 10089.5 37 11279.5 38 10788.0 39 9582.5 40 8186.0 41 6990.0 42 5661.5 43 5156.5 44 5332.5 45 4936.5 46 3919.5 47 3310.0 48 3557.5 49 3002.0 50 1986.0 51 1308.0 52 913.0 53 704.0 54 591.0 55 519.0 56 426.5 57 346.5 58 309.5 59 275.0 60 262.5 61 248.5 62 178.0 63 95.0 64 47.0 65 31.5 66 16.5 67 17.5 68 17.5 69 11.0 70 8.0 71 9.0 72 6.5 73 3.0 74 3.5 75 2.5 76 1.0 77 0.0 78 0.5 79 1.5 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043359051201938406 2 0.036982720142829814 3 0.02231715870688006 4 0.042721418096027544 5 0.059937511955620734 6 0.06567620990881846 7 0.0682267423324619 8 0.0682267423324619 9 0.06567620990881846 10-11 0.0714149078620162 12-13 0.06950200854428362 14-15 0.06918319199132819 16-17 0.051967098131735 18-19 0.04590958362558184 20-21 0.05579289676720015 22-23 0.027099407001211502 24-25 0.01657846075368233 26-27 0.013071478671172608 28-29 0.015303194541860615 30-31 0.008926863482752024 32-33 0.006695147612064018 34-35 0.006695147612064018 36-37 0.0035069820825097235 38-39 0.0051010648472868715 40-41 0.004144615188420583 42-43 0.0035069820825097235 44-45 0.0012752662118217179 46-47 9.564496588662884E-4 48-49 6.376331059108589E-4 50-51 0.004144615188420583 52-53 0.0025505324236434358 54-55 0.0038257986354651536 56-57 0.01785372696550405 58-59 0.007651597270930307 60-61 0.014346744882994325 62-63 0.010520946247529172 64-65 0.007651597270930307 66-67 0.014027928330038894 68-69 0.024548874577568068 70-71 0.013071478671172608 72-73 0.012115029012306319 74-75 0.01721609385959319 76-77 0.020723075942102914 78-79 0.03283810495440923 80-81 0.026142957342345216 82-83 0.027099407001211502 84-85 0.01912899317732577 86-87 0.020404259389147486 88-89 0.01785372696550405 90-91 0.01817254351845948 92-93 0.02901230631894408 94-95 0.01912899317732577 96-97 0.04080851877829497 98-99 0.052923547790601294 100-101 0.06535739335586303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 156830.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.574507428425683 #Duplication Level Percentage of deduplicated Percentage of total 1 75.36600625651721 23.042785181406618 2 8.861313868613138 5.41860613403048 3 3.56830031282586 3.2729707326404385 4 2.173096976016684 2.65765478543646 5 1.4494264859228363 2.2157750430402348 6 0.9322210636079249 1.7101319900529235 7 0.8842544316996872 1.8924950583434292 8 0.6527632950990615 1.5966332972007908 9 0.5338894681960376 1.4691066760186189 >10 4.798748696558915 30.34814767582733 >50 0.5818561001042752 12.217050309252055 >100 0.18769551616266944 8.7374864502965 >500 0.0020855057351407717 0.6267933431103743 >1k 0.008342022940563087 4.794363323343748 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2380 1.5175667920678442 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1973 1.2580501179621246 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1782 1.1362621947331504 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.8824842185806286 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 983 0.6267933431103743 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 321 0.2046802269973857 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 314 0.2002167952560097 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 305 0.19447809730281196 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 0.19001466556143595 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 292 0.18618886692597078 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 274 0.17471147101957535 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 241 0.153669578524517 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 234 0.14920614678314098 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 233 0.14856851367723012 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 214 0.1364534846649238 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 212 0.13517821845310207 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 205 0.13071478671172607 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 204 0.1300771536058152 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 199 0.12688898807626092 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 194 0.12370082254670661 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 194 0.12370082254670661 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 192 0.12242555633488492 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 186 0.11859975769941974 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 186 0.11859975769941974 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 180 0.1147739590639546 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 180 0.1147739590639546 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 178 0.11349869285213288 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 178 0.11349869285213288 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 176 0.11222342664031115 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.1115857935344003 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 169 0.10775999489893516 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 169 0.10775999489893516 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 164 0.10457182936938086 No Hit GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT 161 0.10265893005164828 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 158 0.1007460307339157 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.376331059108589E-4 7 0.0 0.0 0.0 0.0 6.376331059108589E-4 8 0.0 0.0 0.0 0.0 6.376331059108589E-4 9 0.0 0.0 0.0 0.0 6.376331059108589E-4 10-11 0.0 0.0 0.0 0.0 6.376331059108589E-4 12-13 0.0 0.0 0.0 0.0 6.376331059108589E-4 14-15 0.0 0.0 0.0 6.376331059108589E-4 6.376331059108589E-4 16-17 0.0 0.0 0.0 6.376331059108589E-4 6.376331059108589E-4 18-19 0.0 0.0 0.0 6.376331059108589E-4 6.376331059108589E-4 20-21 0.0 0.0 0.0 6.376331059108589E-4 6.376331059108589E-4 22-23 0.0 0.0 0.0 0.0025505324236434353 6.376331059108589E-4 24-25 0.0 0.0 0.0 0.004463431741376012 6.376331059108589E-4 26-27 0.0 0.0 0.0 0.010839762800484602 6.376331059108589E-4 28-29 0.0 0.0 0.0 0.023911241471657206 6.376331059108589E-4 30-31 0.0 0.0 0.0 0.04718484983740356 6.376331059108589E-4 32-33 0.0 0.0 0.0 0.07492188994452592 6.376331059108589E-4 34-35 0.0 0.0 0.0 0.09660141554549512 6.376331059108589E-4 36-37 0.0 0.0 0.0 0.11828094114646433 6.376331059108589E-4 38-39 0.0 0.0 0.0 0.1415545495122107 6.376331059108589E-4 40-41 0.0 0.0 0.0 0.15526366128929414 6.376331059108589E-4 42-43 0.0 0.0 0.0 0.2136070904801377 6.376331059108589E-4 44-45 0.0 0.0 0.0 0.28725371421284196 6.376331059108589E-4 46-47 0.0 0.0 0.0 0.35356755722757127 6.376331059108589E-4 48-49 0.0 0.0 0.0 0.43805394376076007 6.376331059108589E-4 50-51 0.0 0.0 0.0 0.548683287636294 6.376331059108589E-4 52-53 0.0 0.0 0.0 0.7406108525154627 6.376331059108589E-4 54-55 0.0 0.0 0.0 0.9089459924759293 6.376331059108589E-4 56-57 0.0 0.0 0.0 1.1675062169227826 6.376331059108589E-4 58-59 0.0 0.0 0.0 1.5207549575973984 6.376331059108589E-4 60-61 0.0 0.0 0.0 1.8347892622584965 6.376331059108589E-4 62-63 0.0 0.0 0.0 2.237773385194159 6.376331059108589E-4 64-65 0.0 0.0 0.0 2.537460944972263 6.376331059108589E-4 66-67 0.0 0.0 0.0 2.9857170184275965 6.376331059108589E-4 68-69 0.0 0.0 0.0 3.5624561627239686 6.376331059108589E-4 70-71 0.0 0.0 0.0 4.052477204616464 6.376331059108589E-4 72-73 0.0 0.0 0.0 4.7117898361282915 6.376331059108589E-4 74-75 0.0 0.0 0.0 5.349741758592106 6.376331059108589E-4 76-77 0.0 0.0 0.0 5.832748836319581 6.376331059108589E-4 78-79 0.0 0.0 0.0 6.36581011286106 6.376331059108589E-4 80-81 0.0 0.0 0.0 7.1810240387680935 6.376331059108589E-4 82-83 0.0 0.0 0.0 7.842568386150609 6.376331059108589E-4 84-85 0.0 0.0 0.0 8.814321239558758 0.0012752662118217179 86-87 0.0 0.0 0.0 9.941656570809156 0.0012752662118217179 88-89 0.0 0.0 0.0 11.305234967799528 0.0012752662118217179 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTGG 20 0.0020684924 71.26938 1 CGCAGAA 65 4.3661203E-8 51.184093 9 GGTATCA 905 0.0 39.37535 1 GGTCCTA 55 0.0025654098 34.565884 8 TGATGTC 30 0.0039413553 31.67528 92-93 CAACTGA 30 0.003953687 31.655079 34-35 GTATCAA 1310 0.0 30.828999 1 GTATAGT 85 6.3721795E-4 27.948778 1 GACTGGT 35 0.008397408 27.158907 7 TATCTCT 35 0.008384351 27.141579 68-69 ATATAGG 90 8.898822E-4 26.396067 2 ATCAACG 1575 0.0 25.943563 3 TCAACGC 1580 0.0 25.861465 4 CAACGCA 1625 0.0 24.860846 5 GAGTACT 1020 0.0 24.692026 12-13 AACGCAG 1650 0.0 24.484165 6 GTACATG 490 0.0 24.241287 1 GTACTTT 1040 0.0 24.221045 14-15 TATCAAC 1680 0.0 24.039276 2 CTTACTA 100 0.0016416413 23.764044 9 >>END_MODULE