FastQCFastQC Report
Thu 26 May 2016
SRR937118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156830
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25271.6112988586367405No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22441.4308486896639672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18051.1509277561691003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.9902442134795639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11090.7071351144551425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.2633424727411847No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4130.2633424727411847No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.25951667410571955No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT2790.17789963654912963No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC2780.17726200344321877No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA2650.1689727730663776No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA2650.1689727730663776No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2400.15303194541860615No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2310.1472932474654084No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG2290.1460179812535867No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2260.1441050819358541No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG2020.12880188739399348No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG1940.12370082254670661No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA1890.12051265701715232No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG1870.11923739080533062No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.1179621245935089No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC1820.11604922527577632No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1800.1147739590639546No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.11349869285213288No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA1770.11286105974622203No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT1760.11222342664031115No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT1710.10903526111075688No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT1700.10839762800484601No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT1670.10648472868711344No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC1650.10520946247529171No Hit
GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG1640.10457182936938086No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.10393419626347No Hit
CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC1620.10329656315755914No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG1580.1007460307339157No Hit
GTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGT1580.1007460307339157No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT301.2922048E-463.388021
GTGGTAC352.7817968E-454.2806136
TGGTACT554.946385E-543.1777577
GGTACTC459.604066E-442.2182548
TATGGTG459.604066E-442.2182542
ATGGTGG550.002574052234.5422063
TGGTGGT550.002574052234.5422064
ACCCTTG1001.2906767E-633.2468767
ACGCAGC600.00394245831.663698
GGTATCA11000.030.2533741
CATGTAG953.4514364E-530.0259061
GGTGGTA650.00583157329.228025
CCCTTGG1153.8394573E-628.9103268
CCTTGGC1153.8394573E-628.9103269
GTATCAA15650.028.251211
AATTTGG350.00838627527.14030688-89
GAGACCC700.00837371227.1403067
TCTCAGG700.00837371227.1403062
GGATGAC950.001222504724.997658
CACCTAC1751.4704892E-724.4262757