##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937118_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156830 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99542179429956 31.0 31.0 34.0 30.0 34.0 2 32.11534782885927 33.0 31.0 34.0 30.0 34.0 3 32.206108525154626 33.0 31.0 34.0 30.0 34.0 4 35.94437288784034 37.0 35.0 37.0 35.0 37.0 5 35.84499776828413 37.0 35.0 37.0 35.0 37.0 6 35.88009947076452 37.0 35.0 37.0 35.0 37.0 7 35.84198176369317 37.0 35.0 37.0 35.0 37.0 8 35.8173244914876 37.0 35.0 37.0 35.0 37.0 9 37.47309188293056 39.0 37.0 39.0 34.0 39.0 10-11 37.385497035006054 39.0 37.0 39.0 34.0 39.0 12-13 37.341325001594086 39.0 37.0 39.0 34.0 39.0 14-15 38.474807115985456 40.0 38.0 41.0 34.0 41.0 16-17 38.38429509660141 40.0 38.0 41.0 34.0 41.0 18-19 38.38707836510872 40.0 37.5 41.0 34.0 41.0 20-21 38.45727858190398 40.0 38.0 41.0 34.0 41.0 22-23 38.361270165146976 40.0 38.0 41.0 34.0 41.0 24-25 38.26972836829688 40.0 37.0 41.0 34.0 41.0 26-27 38.058901358158515 40.0 37.0 41.0 33.5 41.0 28-29 37.89172989861633 40.0 37.0 41.0 33.0 41.0 30-31 37.72183893387745 40.0 36.5 41.0 33.0 41.0 32-33 37.49666517885609 39.5 35.5 41.0 33.0 41.0 34-35 37.332407702607924 39.0 35.0 41.0 33.0 41.0 36-37 37.110871644455784 39.0 35.0 41.0 32.5 41.0 38-39 36.94979276924058 39.0 35.0 41.0 32.0 41.0 40-41 36.719702862972646 39.0 35.0 41.0 31.0 41.0 42-43 36.41878148313461 39.0 35.0 41.0 31.0 41.0 44-45 36.09150353886373 39.0 35.0 41.0 30.0 41.0 46-47 35.604524006886436 39.0 35.0 41.0 28.0 41.0 48-49 35.136434355671746 39.0 35.0 40.0 25.5 41.0 50-51 34.84278199324109 39.0 35.0 40.0 24.0 41.0 52-53 34.665826053688704 38.5 35.0 40.0 24.0 41.0 54-55 34.3843907415673 38.0 34.5 40.0 23.0 41.0 56-57 34.117812280813624 38.0 34.0 40.0 21.0 41.0 58-59 33.78479563858956 38.0 34.0 40.0 19.0 41.0 60-61 33.45002869348977 38.0 33.0 40.0 15.0 41.0 62-63 33.093457884333354 37.5 33.0 40.0 10.0 41.0 64-65 32.68905821590257 37.0 32.5 39.5 6.5 41.0 66-67 32.233867882420455 36.5 32.0 39.0 2.0 40.0 68-69 31.7451348594019 36.0 31.0 39.0 2.0 40.0 70-71 31.34264171395779 36.0 31.0 39.0 2.0 40.0 72-73 30.932095262386024 35.0 31.0 38.0 2.0 40.0 74-75 30.421991965822865 35.0 30.0 37.5 2.0 39.5 76-77 28.92610151119046 33.5 28.5 36.0 2.0 38.5 78-79 29.564180960275458 34.0 29.0 36.0 2.0 39.0 80-81 29.091376012242556 34.0 29.0 36.0 2.0 38.0 82-83 28.904415609258432 34.0 29.0 36.0 2.0 37.0 84-85 28.516642224064274 34.0 29.0 35.0 2.0 37.0 86-87 28.13782439584263 34.0 29.0 35.0 2.0 36.0 88-89 27.71574316138494 34.0 27.5 35.0 2.0 36.0 90-91 27.259918382962443 34.0 26.5 35.0 2.0 36.0 92-93 26.797618440349424 33.0 26.0 35.0 2.0 35.0 94-95 26.29248868201237 33.0 25.0 35.0 2.0 35.0 96-97 25.664783523560544 32.5 23.0 34.5 2.0 35.0 98-99 24.975651979850795 32.0 11.0 34.0 2.0 35.0 100-101 22.140177262003444 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19432061461473538 1101 2 0.15183716470031072 1101 3 0.11442140449372573 1101 4 0.23571876309827644 1101 5 0.2678602640064369 1101 6 0.21738721334195787 1101 7 0.08898670068347059 1101 8 0.17366509595635193 1101 9 0.2363156706265599 1101 10-11 0.26113863109073066 1101 12-13 -0.09528189313746793 1101 14-15 0.16286854107455895 1101 16-17 0.1972873312898784 1101 18-19 0.16181360491997054 1101 20-21 0.2668426355645437 1101 22-23 0.12137799672148475 1101 24-25 0.3913395338321237 1101 26-27 0.5864748365040597 1101 28-29 0.2814140818725619 1101 30-31 0.35363995564081563 1101 32-33 0.45178626705757097 1101 34-35 0.662800346290183 1101 36-37 0.8255745128206797 1101 38-39 0.32961100253572084 1101 40-41 0.5582266020069468 1101 42-43 0.4660830352699392 1101 44-45 0.8360866699182878 1101 46-47 1.1314849232718416 1101 48-49 1.1611888145767608 1101 50-51 1.0476149954325464 1101 52-53 1.1159744775867253 1101 54-55 1.0367229147216435 1101 56-57 1.0866135197294966 1101 58-59 1.0842689380091812 1101 60-61 1.185487770312001 1101 62-63 1.47947546765338 1101 64-65 1.4171105949748508 1101 66-67 1.4452596761772405 1101 68-69 1.6116057337244598 1101 70-71 1.9066915606297705 1101 72-73 1.9044932259443037 1101 74-75 1.7934970081494335 1101 76-77 1.9391446177930085 1101 78-79 2.1473989335655155 1101 80-81 2.5221651169920882 1101 82-83 2.357957197720232 1101 84-85 1.9855487991722853 1101 86-87 1.8740690859821356 1101 88-89 1.9971100514164704 1101 90-91 1.67979330827119 1101 92-93 1.8774465133503426 1101 94-95 2.034812008567176 1101 96-97 1.7251803932895982 1101 98-99 1.8771892999551198 1101 100-101 1.728429825277292 1102 1 0.4165791402426713 1102 2 0.28447959884366725 1102 3 0.4052831565868402 1102 4 0.23613738674540485 1102 5 0.18427847924025542 1102 6 0.27954771977446313 1102 7 0.25654847319725604 1102 8 0.23767367263594963 1102 9 0.3186620051170621 1102 10-11 0.4052370586994698 1102 12-13 0.435859622075931 1102 14-15 0.2140937810173824 1102 16-17 0.12174618364846168 1102 18-19 2.044564617236233E-4 1102 20-21 -0.033290098762591924 1102 22-23 -0.0406140392188874 1102 24-25 -0.008451154344307099 1102 26-27 0.05730391553203873 1102 28-29 -0.1955922485338064 1102 30-31 0.3905094675869023 1102 32-33 0.30065802527688845 1102 34-35 0.18570722805452533 1102 36-37 0.26803418200589135 1102 38-39 0.6066581742735195 1102 40-41 0.649787761900761 1102 42-43 0.7083129719658743 1102 44-45 0.6963888957708448 1102 46-47 1.0686964813686401 1102 48-49 1.3172435419608775 1102 50-51 0.787695657062109 1102 52-53 0.8122885474254034 1102 54-55 0.7500269784341143 1102 56-57 0.860663958773813 1102 58-59 0.8879344475047901 1102 60-61 1.164959896103305 1102 62-63 1.141778918745878 1102 64-65 1.3150174767391931 1102 66-67 1.5904557145680904 1102 68-69 1.0389847412801032 1102 70-71 1.103710143435574 1102 72-73 1.578931657637174 1102 74-75 1.8213814271814925 1102 76-77 1.744295730719287 1102 78-79 1.8585281963794849 1102 80-81 1.955680534594702 1102 82-83 1.9818953231031209 1102 84-85 1.914474672155741 1102 86-87 1.5764327780384981 1102 88-89 1.7690469515594138 1102 90-91 2.012813524730248 1102 92-93 2.2015326885596593 1102 94-95 2.228813438013777 1102 96-97 2.016384191533419 1102 98-99 2.4026691855993896 1102 100-101 3.015876221029796 1103 1 0.06763204214302831 1103 2 -0.021401243477853882 1103 3 0.04692414271935519 1103 4 0.15836388905665189 1103 5 0.1244685151930014 1103 6 0.09033354411960914 1103 7 0.10642007052647529 1103 8 -0.07592417019917974 1103 9 0.11816123470103435 1103 10-11 0.08322242079500342 1103 12-13 -0.001383572582518866 1103 14-15 -0.003022294996995356 1103 16-17 0.08668583439320088 1103 18-19 0.152663367607083 1103 20-21 0.16470681957331124 1103 22-23 0.11932432749913602 1103 24-25 0.5174348240840487 1103 26-27 0.3439821384390811 1103 28-29 0.03814555321247326 1103 30-31 0.4309948296824402 1103 32-33 0.6915863765865993 1103 34-35 0.577983807407378 1103 36-37 0.6614407048615618 1103 38-39 0.6367942811045424 1103 40-41 0.5618319324195014 1103 42-43 0.23196474392273814 1103 44-45 0.10488915257619169 1103 46-47 0.5014648429505613 1103 48-49 0.05179670066657849 1103 50-51 0.3171704901386434 1103 52-53 0.42295238923331624 1103 54-55 0.41140345506483555 1103 56-57 0.4599705843516233 1103 58-59 0.3504472877718712 1103 60-61 0.3309331883477782 1103 62-63 0.8232825140206614 1103 64-65 0.2735819348799211 1103 66-67 0.33235350604215697 1103 68-69 0.31617400681682994 1103 70-71 0.4625057263837 1103 72-73 1.2041692025781074 1103 74-75 1.2906675083319818 1103 76-77 1.2648337379098358 1103 78-79 0.7620849503599665 1103 80-81 1.1031253214462673 1103 82-83 1.2037494576691152 1103 84-85 0.8786439068758263 1103 86-87 0.7859666763435804 1103 88-89 1.034943202201429 1103 90-91 0.9883913367487374 1103 92-93 1.025242241718363 1103 94-95 1.2962441005715597 1103 96-97 2.082883934728077 1103 98-99 1.9515777628978022 1103 100-101 2.07529455693118 1104 1 0.3881136438746289 1104 2 0.1781387998121886 1104 3 0.25593388297909314 1104 4 0.22695155139431478 1104 5 0.14624881822330593 1104 6 0.36427834931441794 1104 7 0.19908781944423026 1104 8 0.27553686876185424 1104 9 0.11126188405168591 1104 10-11 0.15079547274304872 1104 12-13 0.2597095010105619 1104 14-15 0.3830707785960712 1104 16-17 0.4911771763845394 1104 18-19 0.4818706186893351 1104 20-21 0.4830915598330563 1104 22-23 0.4401441326939377 1104 24-25 0.47400949940871584 1104 26-27 0.45714501722695644 1104 28-29 0.5524177393596617 1104 30-31 0.8311571673447773 1104 32-33 0.6436967661969817 1104 34-35 0.5927971190956924 1104 36-37 0.5795278260736865 1104 38-39 0.7716021815374461 1104 40-41 0.6977220839346572 1104 42-43 0.6134582504162367 1104 44-45 0.24260560279263643 1104 46-47 0.42408389056960516 1104 48-49 0.5970483240431932 1104 50-51 0.43479765464080344 1104 52-53 0.49140477018568873 1104 54-55 0.761308758095133 1104 56-57 0.8286799999360355 1104 58-59 1.0643947985943782 1104 60-61 0.832083080122672 1104 62-63 0.5928306741938272 1104 64-65 0.661839510637499 1104 66-67 0.8136170341806839 1104 68-69 0.8980192449120636 1104 70-71 0.8937827826607538 1104 72-73 1.011528509937417 1104 74-75 1.1643823351718083 1104 76-77 1.2584755128016134 1104 78-79 1.5389383053383199 1104 80-81 1.728463524909472 1104 82-83 1.8022343061539665 1104 84-85 1.9775345995165168 1104 86-87 2.018109533486438 1104 88-89 1.2680330290412556 1104 90-91 1.7678150380474413 1104 92-93 1.7768656183417377 1104 94-95 1.8828918278442863 1104 96-97 1.6282031987973404 1104 98-99 1.5361286286986662 1104 100-101 1.973427673814296 1105 1 -0.14070129119030383 1105 2 -0.13314366772027952 1105 3 -0.180348584553375 1105 4 -0.1200452018524345 1105 5 -0.11795572723124081 1105 6 -0.12557554678947724 1105 7 -0.2704738688674624 1105 8 -0.12137871565372649 1105 9 -0.6375205834807787 1105 10-11 -0.3599593973868167 1105 12-13 -0.25611842106737015 1105 14-15 0.012129220154520226 1105 16-17 -0.07619295348558808 1105 18-19 -0.08691996572625271 1105 20-21 -6.590769941539065E-5 1105 22-23 -0.136357490682677 1105 24-25 -0.366087903188685 1105 26-27 -0.1664345282275903 1105 28-29 -0.4770059619390423 1105 30-31 -0.26786880668120006 1105 32-33 -0.3368227143224942 1105 34-35 -0.2572434653198954 1105 36-37 -0.1747335375626733 1105 38-39 0.21823367504394042 1105 40-41 0.014483447571016939 1105 42-43 0.3086692893772778 1105 44-45 0.48935884954588005 1105 46-47 0.8546845399202496 1105 48-49 0.7932739733938448 1105 50-51 0.42039018710833886 1105 52-53 0.5413236013545273 1105 54-55 0.5951155762769531 1105 56-57 0.7184933116243499 1105 58-59 0.6534775908021686 1105 60-61 0.607448339862934 1105 62-63 0.6859719079600595 1105 64-65 0.42888496518295227 1105 66-67 0.7083004757391258 1105 68-69 0.7101134007562209 1105 70-71 0.8024678475958176 1105 72-73 1.253411626820533 1105 74-75 0.905250841665314 1105 76-77 0.7970307076068046 1105 78-79 1.4060243442993574 1105 80-81 1.2262313820523296 1105 82-83 1.0437115788812399 1105 84-85 0.9714469371788574 1105 86-87 0.5899439490708538 1105 88-89 0.7120265355347613 1105 90-91 0.7582777003851042 1105 92-93 1.0081967871729063 1105 94-95 0.7990850096624662 1105 96-97 1.573414237758378 1105 98-99 1.3019565507765876 1105 100-101 0.6150672842039064 1106 1 0.16936364387462888 1106 2 0.08357711150050307 1106 3 0.1686773894725988 1106 4 -0.03806468237191751 1106 5 0.19048583121031726 1106 6 0.012411466197534082 1106 7 -0.14466218055576974 1106 8 -0.06780728708229589 1106 9 -0.23735824581844156 1106 10-11 0.2187743688469439 1106 12-13 -0.08444634314528798 1106 14-15 0.0332331162584083 1106 16-17 0.4733200335274006 1106 18-19 0.3931936706373804 1106 20-21 -0.26386460899811937 1106 22-23 -0.17288346470865434 1106 24-25 0.3031491097983263 1106 26-27 0.06124404320097909 1106 28-29 -0.14664881908189642 1106 30-31 -0.11283633914873548 1106 32-33 0.0024629999632139743 1106 34-35 0.08204224896581991 1106 36-37 0.04969828061913972 1106 38-39 -0.32803255872229187 1106 40-41 0.03152890211647019 1106 42-43 -0.35894434698635536 1106 44-45 0.16549521318225402 1106 46-47 -0.3145524730667617 1106 48-49 -0.42709940322952633 1106 50-51 -0.7171909817228297 1106 52-53 -0.3965822428013226 1106 54-55 -0.16868312502175087 1106 56-57 0.060619935000971736 1106 58-59 0.5406529154774944 1106 60-61 0.4662145736291663 1106 62-63 0.5021245053626515 1106 64-65 0.5944693807673715 1106 66-67 0.6802972289858751 1106 68-69 0.8424185955614156 1106 70-71 0.4197568086347836 1106 72-73 0.7672103281192335 1106 74-75 0.7993255169899918 1106 76-77 0.7077449933210929 1106 78-79 0.7777775910526046 1106 80-81 1.4056144989354458 1106 82-83 0.5847018386215019 1106 84-85 0.5087845995165168 1106 86-87 1.2705445984215018 1106 88-89 0.6799648472230722 1106 90-91 0.6539757523331531 1106 92-93 -0.012907108930988898 1106 94-95 0.15582202264948108 1106 96-97 0.5368882637324077 1106 98-99 0.21185914817918317 1106 100-101 -0.5099327157960936 1107 1 0.26576724027822607 1107 2 0.021139549062937135 1107 3 0.21912693992214827 1107 4 0.3550422107349789 1107 5 0.19148483220931922 1107 6 -5.755467894772437E-4 1107 7 0.30338976749617785 1107 8 0.34278212350711357 1107 9 0.3620423535821544 1107 10-11 0.10988325995584347 1107 12-13 0.2100092013102568 1107 14-15 -9.82667957373451E-4 1107 16-17 0.4023909625983251 1107 18-19 0.4928440202877269 1107 20-21 0.04232919719568429 1107 22-23 -0.01004630187149047 1107 24-25 0.10135090800012847 1107 26-27 0.2730322549891966 1107 28-29 0.5409136184805377 1107 30-31 0.7902605639481735 1107 32-33 0.7392262367264522 1107 34-35 0.8065677234912911 1107 36-37 0.4517961827170467 1107 38-39 0.6085308778411402 1107 40-41 1.089221209808784 1107 42-43 1.265181527139518 1107 44-45 1.3036071012941335 1107 46-47 1.2159170574027698 1107 48-49 1.8253980992679786 1107 50-51 2.1229688584370123 1107 52-53 1.7622589160398405 1107 54-55 2.7424057860671596 1107 56-57 2.0106698850509233 1107 58-59 2.2614321362567154 1107 60-61 2.008672116086707 1107 62-63 1.7106659639041126 1107 64-65 1.5897241260221122 1107 66-67 1.635092433781086 1107 68-69 1.1895714427142678 1107 70-71 1.269906658784631 1107 72-73 1.3683591792680865 1107 74-75 1.2883365060009773 1107 76-77 1.5356670712431715 1107 78-79 2.2006047638797774 1107 80-81 2.1960740393949862 1107 82-83 1.7253112292308899 1107 84-85 1.7223210630529806 1107 86-87 1.7907743686512738 1107 88-89 1.700444367702591 1107 90-91 2.025104623462024 1107 92-93 2.138441542417663 1107 94-95 2.537939904767363 1107 96-97 2.1315436083877515 1107 98-99 2.1756453619653975 1107 100-101 1.7155917597283832 1108 1 0.5026969772079646 1108 2 0.12109514901853657 1108 3 0.17012039091559927 1108 4 0.3226856783784413 1108 5 0.28067342139790696 1108 6 0.14156009534616487 1108 7 0.1489886130950211 1108 8 0.1277194084443991 1108 9 -0.21066271912291512 1108 10-11 0.5210831711557518 1108 12-13 0.22796346926452316 1108 14-15 0.41382474685003956 1108 16-17 0.5010978113051792 1108 18-19 0.14680117424488515 1108 20-21 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2316 68-69 -0.6336161610488773 2316 70-71 -0.8934731268973124 2316 72-73 -0.8446508323030777 2316 74-75 -0.29718947880558133 2316 76-77 -0.6003695540020892 2316 78-79 -0.7748945569432841 2316 80-81 -0.22385854600522137 2316 82-83 -0.6222261255007098 2316 84-85 -0.6112051229911906 2316 86-87 -0.5634038272535413 2316 88-89 0.13413657477609675 2316 90-91 -0.8593849876462905 2316 92-93 -0.6253055019049185 2316 94-95 -0.7080479203571528 2316 96-97 -0.7914075411821031 2316 98-99 -0.08125960357266138 2316 100-101 -0.4561393868229082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4.0 5 11.0 6 24.0 7 39.0 8 77.0 9 109.0 10 226.0 11 420.0 12 620.0 13 784.0 14 987.0 15 1471.0 16 1952.0 17 2730.0 18 2229.0 19 1429.0 20 1267.0 21 1239.0 22 1328.0 23 1524.0 24 1630.0 25 1709.0 26 1865.0 27 2119.0 28 2640.0 29 3143.0 30 3676.0 31 4452.0 32 5746.0 33 7166.0 34 9709.0 35 13349.0 36 18932.0 37 31961.0 38 28075.0 39 2188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.300321444971686 25.47706515638553 18.86129394356855 19.361319455074238 2 13.928248032751341 25.960030098585623 40.51958321111097 19.592138657552066 3 15.507741754457566 31.14111164961865 34.04713924954723 19.304007346376554 4 10.837281370154754 22.938359614612093 42.17204726166717 24.052311753565984 5 9.815461919094016 40.80179054226394 37.956562771004435 11.426184767637606 6 23.36542753299751 44.18414844098705 19.492444047694956 12.957979978320475 7 21.427022891028503 37.02225339539629 26.870496716189507 14.680226997385704 8 22.00535611808965 36.8647580182363 23.713575208824842 17.416310654849198 9 23.732704202002168 19.22782630874195 25.572913345660908 31.466556143594975 10-11 20.15080022954792 30.569725180131353 31.368998278390613 17.910476311930115 12-13 22.110636072932706 29.662786656847086 31.085031833934305 17.141545436285902 14-15 19.96716189504559 31.38079449084997 29.819231014474273 18.832812599630174 16-17 16.282280175986738 33.92463176688133 31.390996620544538 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16.362227670842408 31.0830673676894 34.86578433029266 17.68892063117553 44-45 16.822412028579354 31.348018351490342 34.68642098114796 17.14314863878235 46-47 17.44314685693699 31.27696001071227 34.51200188740001 16.76789124495073 48-49 17.31753273459394 31.55496893122786 33.8466296200651 17.280868714113097 50-51 17.062548223156043 31.36976081314603 33.46734853943618 18.100342424261747 52-53 17.180122362183134 31.867410149238506 33.71814613959746 17.2343213489809 54-55 18.03620503860893 31.50342092342615 33.08189174196099 17.378482296003927 56-57 17.999107313651724 31.71650832111203 32.237454568641205 18.04692979659504 58-59 17.248294331441688 31.54657909838679 33.29879487342983 17.906331696741695 60-61 17.95192246381432 32.354141427022896 32.147548300707776 17.546387808455016 62-63 17.434802014920614 32.201747114710194 33.30772173691258 17.05572913345661 64-65 17.494994324775863 31.69230082513933 33.018007677494225 17.79469717259058 66-67 17.53012816425429 32.18995090225085 31.926289612956705 18.353631320538163 68-69 17.15966332972008 31.457629280112226 32.82981572403239 18.552891666135306 70-71 17.764160508075776 31.478316871457086 32.857543662762154 17.899978957704988 72-73 17.276365085691513 31.08043045037864 32.62654444001594 19.016660023913907 74-75 17.23578324469781 31.94226575352948 31.733111425692822 19.088839576079884 76-77 16.955788440017727 31.49902279922589 32.418197295720404 19.12699146503598 78-79 17.066770399232208 30.63320441160997 32.35913999751297 19.940885191644853 80-81 18.067015239431232 31.623732704202002 30.828923037684113 19.48032901868265 82-83 17.931566596949448 30.99908177319798 31.46584706422486 19.60350456562771 84-85 16.90498279979213 30.91988433298582 31.132216834205302 21.04291603301675 86-87 16.794937193139067 31.200663138430144 31.41458904546324 20.589810622967544 88-89 17.372480129568284 30.375856429156688 31.643483167919094 20.608180273355927 90-91 16.932665944015813 29.60179812535867 32.65606070267169 20.809475227953836 92-93 17.457812465129326 30.844962204177147 31.340723524591997 20.35650180610153 94-95 17.288311754559917 29.787634262905023 31.701827155140805 21.222226827394255 96-97 17.33883823248103 29.96301727985717 31.302684435375884 21.395460052285912 98-99 16.915131033603263 30.829241854237072 30.906395460052288 21.349231652107377 100-101 16.604390769564304 30.801063578802392 30.59319386082931 22.001351790803998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2001.0 1 1256.0 2 476.0 3 395.5 4 311.5 5 301.5 6 378.0 7 564.5 8 722.0 9 747.0 10 620.5 11 496.5 12 514.5 13 527.5 14 541.5 15 540.0 16 558.5 17 584.5 18 586.0 19 571.5 20 587.0 21 651.5 22 714.5 23 823.5 24 913.0 25 1057.5 26 1444.0 27 1871.5 28 1942.0 29 2130.0 30 2953.0 31 3659.5 32 4654.5 33 6131.5 34 7187.0 35 8359.0 36 10005.0 37 10962.0 38 10558.5 39 9394.0 40 8149.0 41 7016.5 42 5628.0 43 5207.5 44 5411.0 45 5047.0 46 4118.0 47 3556.0 48 3770.0 49 3165.5 50 2113.0 51 1346.5 52 910.0 53 729.5 54 565.0 55 434.0 56 376.0 57 318.0 58 283.5 59 255.0 60 234.0 61 216.0 62 142.5 63 68.5 64 25.5 65 13.0 66 11.0 67 9.5 68 5.0 69 5.0 70 4.5 71 2.0 72 1.0 73 0.5 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024230058024612637 2 0.007651597270930307 3 0.01147739590639546 4 6.376331059108589E-4 5 0.0038257986354651536 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.1881655295542947E-4 14-15 0.0 16-17 0.0 18-19 0.0035069820825097235 20-21 6.376331059108589E-4 22-23 0.0 24-25 0.004144615188420583 26-27 0.006057514506153159 28-29 0.008289230376841166 30-31 0.02837467321303322 32-33 3.1881655295542947E-4 34-35 0.002869348976598865 36-37 0.01689727730663776 38-39 0.006057514506153159 40-41 0.005419881400242301 42-43 0.007332780717974878 44-45 0.002231715870688006 46-47 3.1881655295542947E-4 48-49 3.1881655295542947E-4 50-51 0.004463431741376012 52-53 3.1881655295542947E-4 54-55 6.376331059108589E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0051010648472868715 66-67 0.0 68-69 0.0 70-71 0.0019128993177325768 72-73 0.01115857935344003 74-75 0.0051010648472868715 76-77 0.002869348976598865 78-79 0.010520946247529172 80-81 0.0 82-83 0.0038257986354651536 84-85 3.1881655295542947E-4 86-87 0.0 88-89 9.564496588662884E-4 90-91 0.0 92-93 3.1881655295542947E-4 94-95 9.564496588662884E-4 96-97 0.0 98-99 0.0 100-101 6.376331059108589E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 156830.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.377670088631003 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22568532546177 21.805776955939553 2 9.224491567729473 5.419881400242301 3 3.791808651487856 3.3418351080788113 4 2.1183773576715215 2.489319645475993 5 1.5323508345451784 2.250844863865332 6 1.1807349206693725 2.0812344576930433 7 0.8855511905020294 1.821080150481413 8 0.7140841707724698 1.6782503347573807 9 0.6077312091680594 1.6068354268953644 >10 4.88138389078202 29.422304406044763 >50 0.6033902719597162 12.239367467958937 >100 0.22355826622967898 9.952815150162596 >500 0.0 0.0 >1k 0.010852343020858203 5.890454632404515 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2527 1.6112988586367405 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2244 1.4308486896639672 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1805 1.1509277561691003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1553 0.9902442134795639 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1109 0.7071351144551425 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 413 0.2633424727411847 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 413 0.2633424727411847 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 407 0.25951667410571955 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 279 0.17789963654912963 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 278 0.17726200344321877 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 265 0.1689727730663776 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 265 0.1689727730663776 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 240 0.15303194541860615 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 231 0.1472932474654084 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 229 0.1460179812535867 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 226 0.1441050819358541 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 202 0.12880188739399348 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 194 0.12370082254670661 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 189 0.12051265701715232 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 187 0.11923739080533062 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 185 0.1179621245935089 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 182 0.11604922527577632 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 180 0.1147739590639546 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.11349869285213288 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 177 0.11286105974622203 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 176 0.11222342664031115 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 171 0.10903526111075688 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 170 0.10839762800484601 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 167 0.10648472868711344 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 165 0.10520946247529171 No Hit GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG 164 0.10457182936938086 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 163 0.10393419626347 No Hit CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC 162 0.10329656315755914 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 158 0.1007460307339157 No Hit GTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGT 158 0.1007460307339157 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.376331059108589E-4 0.0 16-17 0.0 0.0 0.0 9.564496588662884E-4 0.0 18-19 0.0 0.0 0.0 0.0012752662118217179 0.0 20-21 0.0 0.0 0.0 0.0012752662118217179 0.0 22-23 0.0 0.0 0.0 0.0031881655295542943 0.0 24-25 0.0 0.0 0.0 0.0051010648472868715 0.0 26-27 0.0 0.0 0.0 0.01147739590639546 0.0 28-29 0.0 0.0 0.0 0.0248676911305235 0.0 30-31 0.0 0.0 0.0 0.048460116049225274 0.0 32-33 0.0 0.0 0.0 0.07683478926225851 0.0 34-35 0.0 0.0 0.0 0.09819549831027227 6.376331059108589E-4 36-37 0.0 0.0 0.0 0.1201938404641969 6.376331059108589E-4 38-39 0.0 0.0 0.0 0.1450615315947204 6.376331059108589E-4 40-41 0.0 0.0 0.0 0.16004590958362558 6.376331059108589E-4 42-43 0.0 0.0 0.0 0.2177517056685583 6.376331059108589E-4 44-45 0.0 0.0 0.0 0.29267359561308426 6.376331059108589E-4 46-47 0.0 0.0 0.0 0.35803098896894725 6.376331059108589E-4 48-49 0.0 0.0 0.0 0.44347382516100237 6.376331059108589E-4 50-51 0.0 0.0 0.0 0.5518714531658484 6.376331059108589E-4 52-53 0.0 0.0 0.0 0.7463495504686604 6.376331059108589E-4 54-55 0.0 0.0 0.0 0.9159599566409488 6.376331059108589E-4 56-57 0.0 0.0 0.0 1.1722884652171142 6.376331059108589E-4 58-59 0.0 0.0 0.0 1.5236243065739974 6.376331059108589E-4 60-61 0.0 0.0 0.0 1.8382962443410062 6.376331059108589E-4 62-63 0.0 0.0 0.0 2.2454249824650896 6.376331059108589E-4 64-65 0.0 0.0 0.0 2.545112542243193 6.376331059108589E-4 66-67 0.0 0.0 0.0 2.9889051839571508 6.376331059108589E-4 68-69 0.0 0.0 0.0 3.5653255117005673 6.376331059108589E-4 70-71 0.0 0.0 0.0 4.04928903908691 6.376331059108589E-4 72-73 0.0 0.0 0.0 4.706051138175094 6.376331059108589E-4 74-75 0.0 0.0 0.0 5.3379455461327545 6.376331059108589E-4 76-77 0.0 0.0 0.0 5.81266339348339 6.376331059108589E-4 78-79 0.0 0.0 0.0 6.337435439648027 6.376331059108589E-4 80-81 0.0 0.0 0.0 7.141171969648664 6.376331059108589E-4 82-83 0.0 0.0 0.0 7.798252885289804 6.376331059108589E-4 84-85 0.0 0.0 0.0 8.767774022827265 6.376331059108589E-4 86-87 0.0 0.0 0.0 9.881400242300579 6.376331059108589E-4 88-89 0.0 0.0 0.0 11.244022189632085 6.376331059108589E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 30 1.2922048E-4 63.38802 1 GTGGTAC 35 2.7817968E-4 54.280613 6 TGGTACT 55 4.946385E-5 43.177757 7 GGTACTC 45 9.604066E-4 42.218254 8 TATGGTG 45 9.604066E-4 42.218254 2 ATGGTGG 55 0.0025740522 34.542206 3 TGGTGGT 55 0.0025740522 34.542206 4 ACCCTTG 100 1.2906767E-6 33.246876 7 ACGCAGC 60 0.003942458 31.66369 8 GGTATCA 1100 0.0 30.253374 1 CATGTAG 95 3.4514364E-5 30.025906 1 GGTGGTA 65 0.005831573 29.22802 5 CCCTTGG 115 3.8394573E-6 28.910326 8 CCTTGGC 115 3.8394573E-6 28.910326 9 GTATCAA 1565 0.0 28.25121 1 AATTTGG 35 0.008386275 27.140306 88-89 GAGACCC 70 0.008373712 27.140306 7 TCTCAGG 70 0.008373712 27.140306 2 GGATGAC 95 0.0012225047 24.99765 8 CACCTAC 175 1.4704892E-7 24.426275 7 >>END_MODULE