##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937117_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 168475 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86606914972548 33.0 31.0 34.0 30.0 34.0 2 32.0065766434189 33.0 31.0 34.0 30.0 34.0 3 31.951078795073453 33.0 31.0 34.0 30.0 34.0 4 35.572589404956226 37.0 35.0 37.0 33.0 37.0 5 35.5857604985903 37.0 35.0 37.0 33.0 37.0 6 35.69771182668052 37.0 35.0 37.0 35.0 37.0 7 35.65964683187416 37.0 35.0 37.0 33.0 37.0 8 35.61753375871791 37.0 35.0 37.0 33.0 37.0 9 37.23577385368749 39.0 37.0 39.0 34.0 39.0 10-11 37.141783647425434 39.0 37.0 39.0 33.5 39.0 12-13 37.10741356284315 39.0 37.0 39.0 33.5 39.0 14-15 38.25440866597418 40.0 38.0 41.0 33.5 41.0 16-17 38.180460008903395 40.0 38.0 41.0 33.0 41.0 18-19 38.13523371420092 40.0 38.0 41.0 33.5 41.0 20-21 38.04699213533165 40.0 38.0 41.0 33.5 41.0 22-23 37.91580353168126 40.0 37.5 41.0 33.0 41.0 24-25 37.99145867339368 40.0 38.0 41.0 33.0 41.0 26-27 37.92689123015284 40.0 37.0 41.0 33.0 41.0 28-29 37.82598011574417 40.0 37.0 41.0 33.0 41.0 30-31 37.61572340109808 40.0 37.0 41.0 33.0 41.0 32-33 37.32977889894643 39.5 35.5 41.0 33.0 41.0 34-35 37.13626650838403 39.0 35.0 41.0 32.5 41.0 36-37 36.95021219765544 39.0 35.0 41.0 32.0 41.0 38-39 36.78957412078944 39.0 35.0 41.0 31.0 41.0 40-41 36.631963199287725 39.0 35.0 41.0 31.0 41.0 42-43 36.36187268140674 39.0 35.0 41.0 30.5 41.0 44-45 36.059053271998806 39.0 35.0 41.0 30.0 41.0 46-47 35.64219913933818 39.0 35.0 41.0 29.0 41.0 48-49 35.31201661967651 39.0 35.0 40.5 27.0 41.0 50-51 34.51514171242024 38.0 34.0 40.0 25.0 40.5 52-53 34.70505416233863 38.5 35.0 40.0 25.5 40.5 54-55 35.11975664045111 39.0 35.0 40.5 25.5 41.0 56-57 35.03984864223179 39.0 35.0 41.0 24.5 41.0 58-59 34.836239798189645 39.0 35.0 41.0 23.0 41.0 60-61 34.6203828461196 39.0 35.0 41.0 22.5 41.0 62-63 34.293099866449026 38.5 35.0 40.0 20.5 41.0 64-65 33.975126873423356 38.0 34.5 40.0 18.0 41.0 66-67 33.58598011574418 38.0 34.0 40.0 12.5 41.0 68-69 33.1807538210417 37.0 34.0 40.0 8.0 41.0 70-71 32.75589553346194 37.0 33.0 39.0 3.5 41.0 72-73 32.316512835732304 36.0 33.0 39.0 2.0 41.0 74-75 31.847217688084285 36.0 32.5 39.0 2.0 40.0 76-77 31.390473364000595 35.0 32.0 37.5 2.0 40.0 78-79 30.928710491170797 35.0 31.5 37.0 2.0 39.0 80-81 30.435073453034576 35.0 31.0 36.5 2.0 39.0 82-83 29.973669683929366 35.0 31.0 36.0 2.0 38.5 84-85 29.491247959637928 35.0 30.0 36.0 2.0 37.0 86-87 29.154313696394123 34.0 30.0 35.0 2.0 37.0 88-89 28.7386466834842 34.0 29.5 35.0 2.0 36.0 90-91 28.317925508235646 34.0 29.0 35.0 2.0 36.0 92-93 27.946730968986493 34.0 29.0 35.0 2.0 36.0 94-95 27.572203591037244 34.0 28.5 35.0 2.0 35.0 96-97 27.101774744027303 34.0 26.5 35.0 2.0 35.0 98-99 26.542326754711382 34.0 25.5 35.0 2.0 35.0 100-101 24.283347677696987 31.5 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5360540189475067 1101 2 0.15950548266463827 1101 3 0.3419436113959762 1101 4 0.2817013833083877 1101 5 0.1043119466351925 1101 6 0.0744004839294874 1101 7 0.2581077773291298 1101 8 0.15530359623392798 1101 9 0.4865630833430785 1101 10-11 0.37796195530128784 1101 12-13 0.5344151352323152 1101 14-15 0.42117117226712963 1101 16-17 0.7469005840715042 1101 18-19 0.5796094038894495 1101 20-21 0.5257403958989215 1101 22-23 0.5825562011496004 1101 24-25 0.5608113408354001 1101 26-27 0.7174624967312795 1101 28-29 0.41856272630369773 1101 30-31 0.520706102629191 1101 32-33 0.5601092450466467 1101 34-35 0.8845982795532805 1101 36-37 0.48190589114758353 1101 38-39 0.8506519641239905 1101 40-41 0.9862700021142032 1101 42-43 0.7987086979231179 1101 44-45 0.7928825791725842 1101 46-47 0.9580182378422961 1101 48-49 1.1499194841440712 1101 50-51 0.7812894634856704 1101 52-53 1.0126211640964158 1101 54-55 1.158889089307678 1101 56-57 1.0608535783245117 1101 58-59 0.906229969016735 1101 60-61 1.3449894701709084 1101 62-63 1.395068732719487 1101 64-65 1.3485908676301008 1101 66-67 1.4173525047508164 1101 68-69 1.4061705141728993 1101 70-71 1.5425262878180774 1101 72-73 1.6492919297436117 1101 74-75 1.4463399698719712 1101 76-77 1.265848613175823 1101 78-79 1.5125300887650717 1101 80-81 1.8146259437063073 1101 82-83 1.659682192433376 1101 84-85 1.6642885734792685 1101 86-87 1.7801140562369007 1101 88-89 1.7871360849333406 1101 90-91 1.9072035252895994 1101 92-93 2.075181479448169 1101 94-95 2.293974052823952 1101 96-97 1.688420495110119 1101 98-99 1.5604028039790734 1101 100-101 1.7803848961942421 1102 1 0.39774778135683064 1102 2 0.44834544413430066 1102 3 0.2676350934380878 1102 4 0.19493930817422012 1102 5 0.18878382538256488 1102 6 0.38386204204935837 1102 7 0.43739182065610294 1102 8 0.4891611519161714 1102 9 0.6065379206703341 1102 10-11 0.5414210364705667 1102 12-13 0.4100368301742279 1102 14-15 0.7408943828669052 1102 16-17 0.6108255678533752 1102 18-19 0.508578913807078 1102 20-21 0.7678672314962398 1102 22-23 0.6708417974852807 1102 24-25 0.2542788750900513 1102 26-27 0.8420717872618653 1102 28-29 0.6876755455059609 1102 30-31 0.6216467541065214 1102 32-33 0.8106056180832795 1102 34-35 0.7289042419272178 1102 36-37 0.7246421352255084 1102 38-39 1.0184751963479357 1102 40-41 1.3030395474168373 1102 42-43 1.099520587285987 1102 44-45 1.4566414499976474 1102 46-47 1.954179947331376 1102 48-49 1.6614736723530044 1102 50-51 1.6600663216900742 1102 52-53 1.6191447835896184 1102 54-55 1.9536167230335266 1102 56-57 2.1154742706911875 1102 58-59 1.9562751831943288 1102 60-61 1.7146596032578572 1102 62-63 1.8735182795947907 1102 64-65 2.3159187097343334 1102 66-67 2.235925702572665 1102 68-69 2.278116099893083 1102 70-71 2.064671405669813 1102 72-73 2.1993224001676452 1102 74-75 2.2010088251669515 1102 76-77 2.324386229405743 1102 78-79 2.2794657470245205 1102 80-81 2.572376243495963 1102 82-83 2.437975455520913 1102 84-85 2.4978954853509414 1102 86-87 2.556569264713577 1102 88-89 2.8948441192961205 1102 90-91 2.4569980956566226 1102 92-93 2.55609814775303 1102 94-95 2.667369440977833 1102 96-97 2.6974977327204535 1102 98-99 2.551271505939006 1102 100-101 2.451117562454634 1103 1 0.23966552680304787 1103 2 0.0978241931318955 1103 3 0.32641616801707585 1103 4 0.3119401100988384 1103 5 0.31945171402960426 1103 6 0.43032784569238913 1103 7 0.4769610725749942 1103 8 0.2703617476315969 1103 9 0.2544471886277151 1103 10-11 0.4331955810919368 1103 12-13 0.5152149720003223 1103 14-15 0.6592756407429547 1103 16-17 0.5473188660547734 1103 18-19 0.7435657393311956 1103 20-21 0.6721288880433818 1103 22-23 0.8139681578931217 1103 24-25 0.5729007103518171 1103 26-27 0.64112093378494 1103 28-29 0.7031985149182631 1103 30-31 0.6672476247675334 1103 32-33 0.9186993287030489 1103 34-35 0.5692238661682509 1103 36-37 0.8826098291194242 1103 38-39 0.9100378037485584 1103 40-41 0.9482165436363346 1103 42-43 0.9854869064519818 1103 44-45 1.1846801715006805 1103 46-47 1.0422255424740001 1103 48-49 1.4375648626382613 1103 50-51 1.3508671007025654 1103 52-53 1.6978078606889468 1103 54-55 1.7612865592117757 1103 56-57 1.9026205646538372 1103 58-59 2.001761493195474 1103 60-61 2.1029572318521943 1103 62-63 2.050067712519521 1103 64-65 2.1226777886666284 1103 66-67 1.9944490156669517 1103 68-69 2.10387506426472 1103 70-71 2.0959235536821836 1103 72-73 2.062697357207398 1103 74-75 2.206960251447157 1103 76-77 2.366011845169293 1103 78-79 2.371028354425139 1103 80-81 2.4740831973280173 1103 82-83 2.2286273056755697 1103 84-85 2.4952720462399327 1103 86-87 2.761821870968589 1103 88-89 2.4261077233744786 1103 90-91 2.420384508762364 1103 92-93 2.4754875394359246 1103 94-95 2.7840781132197883 1103 96-97 2.6892264530778824 1103 98-99 3.206168974148426 1103 100-101 2.900584855386242 1104 1 0.3402626308484251 1104 2 0.29109184502023666 1104 3 0.3326708328924326 1104 4 0.26188786349487003 1104 5 0.30667368745847057 1104 6 0.18049951310543833 1104 7 0.14744920513209792 1104 8 0.322444147989863 1104 9 0.5209645792830386 1104 10-11 0.25178044450439074 1104 12-13 0.5159464244559118 1104 14-15 0.5311893592083976 1104 16-17 0.42362129925376024 1104 18-19 0.3134716954440506 1104 20-21 0.6295404420066575 1104 22-23 0.6702377503696084 1104 24-25 0.5663698848554759 1104 26-27 0.7004730758957081 1104 28-29 0.603108949311455 1104 30-31 0.28091384360616445 1104 32-33 0.48034285758794937 1104 34-35 0.3390925032782732 1104 36-37 0.4604721966369638 1104 38-39 0.6564999622796819 1104 40-41 0.7029187379937056 1104 42-43 1.082053558191049 1104 44-45 1.2434128181643587 1104 46-47 1.0031077637010526 1104 48-49 0.9909310366940858 1104 50-51 0.6904700265123864 1104 52-53 0.6383736167719434 1104 54-55 0.9570396566890125 1104 56-57 0.8352970835372489 1104 58-59 0.6337140234238632 1104 60-61 0.7959039403161441 1104 62-63 1.2190332297609032 1104 64-65 0.8714910443764339 1104 66-67 0.9614366257580045 1104 68-69 0.9886265197058322 1104 70-71 1.301357200495147 1104 72-73 0.9332899829724397 1104 74-75 0.8384798812426482 1104 76-77 0.6490615540810687 1104 78-79 0.8742004696662242 1104 80-81 0.6377643437677847 1104 82-83 0.898712393002036 1104 84-85 0.6125134255502758 1104 86-87 0.6756672210208343 1104 88-89 1.0208382801740008 1104 90-91 1.0889320531719768 1104 92-93 0.9929752241649901 1104 94-95 0.9584698134735561 1104 96-97 0.3484591743795704 1104 98-99 0.5390395518465887 1104 100-101 0.7638629565953785 1105 1 -0.33521132048759483 1105 2 0.08747936554568625 1105 3 -0.12038186153957398 1105 4 0.25726030714317716 1105 5 0.1706832411232 1105 6 -0.05341599667214325 1105 7 -0.14766946929692182 1105 8 -0.1961407154225867 1105 9 0.1401614743420012 1105 10-11 -0.04020343368638635 1105 12-13 -0.09414463391100725 1105 14-15 0.1476992860631512 1105 16-17 0.18007748674443036 1105 18-19 0.2218908624839031 1105 20-21 0.1716362772059412 1105 22-23 0.3413080593709523 1105 24-25 0.08152140000699148 1105 26-27 0.009101229351450968 1105 28-29 0.03817388437638414 1105 30-31 -0.3738853801585691 1105 32-33 0.2544136144173308 1105 34-35 -0.017229335802184664 1105 36-37 0.1668462823213943 1105 38-39 0.24427425695053273 1105 40-41 0.011024425409736693 1105 42-43 -0.3061387093115542 1105 44-45 -0.356452833425422 1105 46-47 -0.11220795506294223 1105 48-49 0.4203234833279126 1105 50-51 0.4863350809981313 1105 52-53 0.12859603802884578 1105 54-55 0.38665601733985255 1105 56-57 0.40754667302821446 1105 58-59 0.01974178876197641 1105 60-61 0.3928587096847096 1105 62-63 0.3569642642436648 1105 64-65 0.25617532561243905 1105 66-67 0.04592684817927051 1105 68-69 0.15633811845191303 1105 70-71 -0.23708137242618932 1105 72-73 -0.2417361403295466 1105 74-75 -0.23220231012919612 1105 76-77 -0.5820177114809546 1105 78-79 -0.30754307414628457 1105 80-81 0.10955117762358313 1105 82-83 -0.05068303915201611 1105 84-85 0.16916367185569214 1105 86-87 0.3206888660424809 1105 88-89 0.03793038347300026 1105 90-91 -0.12296524493222094 1105 92-93 -0.13756664775619498 1105 94-95 0.1523047142050089 1105 96-97 0.3192757141616269 1105 98-99 -0.46723004062990015 1105 100-101 -0.5614841101309977 1106 1 -0.5580420439925611 1106 2 -0.2989587003375753 1106 3 -0.19215443494959672 1106 4 -0.21314176237274296 1106 5 0.2756451257036545 1106 6 -0.011282633983974222 1106 7 0.036660552443997574 1106 8 0.32200882911878637 1106 9 -0.29542759465103785 1106 10-11 -0.04873468282637816 1106 12-13 0.4261377229153638 1106 14-15 0.3416200830469407 1106 16-17 -0.06457672246381918 1106 18-19 -0.1527548154751983 1106 20-21 -0.16647639032301953 1106 22-23 -0.4267658047345009 1106 24-25 -0.17266854412709165 1106 26-27 -0.4843076926839416 1106 28-29 -0.26270400868027366 1106 30-31 -0.3974701560068894 1106 32-33 -0.38855360907387393 1106 34-35 -0.16316873641313379 1106 36-37 0.017631982847028382 1106 38-39 -0.19534651526511482 1106 40-41 -0.2874316936975063 1106 42-43 -0.42308562292578245 1106 44-45 0.3766566692853104 1106 46-47 0.34603020438885324 1106 48-49 0.1295317283674038 1106 50-51 0.05901581963918545 1106 52-53 -0.27567509057284667 1106 54-55 0.5601981369424678 1106 56-57 0.7197078310764837 1106 58-59 0.4168163959117095 1106 60-61 0.31494914092559156 1106 62-63 0.4843963582525248 1106 64-65 -0.1068155652150935 1106 66-67 0.4342775650793911 1106 68-69 0.44081399703297564 1106 70-71 0.1318125924030511 1106 72-73 0.36194605687990844 1106 74-75 0.24268554522485175 1106 76-77 0.17314919828044495 1106 78-79 0.14664962200364684 1106 80-81 0.6749115540993529 1106 82-83 0.030033585775743887 1106 84-85 -0.038324563276539436 1106 86-87 0.29036165135772407 1106 88-89 0.9439077619026968 1106 90-91 1.0261252138288235 1106 92-93 0.9375083446757628 1106 94-95 1.0554612763868079 1106 96-97 0.5958533072485643 1106 98-99 0.4516130119060833 1106 100-101 -0.14682412168946257 1107 1 0.08223763697437647 1107 2 0.05299660692499586 1107 3 0.23882128621269771 1107 4 -0.07316073570467552 1107 5 0.1252639022751012 1107 6 0.09538965441067404 1107 7 -0.14667805125931466 1107 8 0.06196877430694059 1107 9 -0.03559835463838823 1107 10-11 -0.09764371624052615 1107 12-13 -0.048090167882570256 1107 14-15 -0.467258733579321 1107 16-17 -0.09112181926294483 1107 18-19 -0.1242069780836843 1107 20-21 -0.05975139822857756 1107 22-23 -0.0327353771318144 1107 24-25 -0.031686147162815814 1107 26-27 -0.21262012021635002 1107 28-29 -0.15095459720492244 1107 30-31 -0.5525420087176087 1107 32-33 -0.13290552924534182 1107 34-35 0.12705297048402286 1107 36-37 -0.3853580737216049 1107 38-39 -0.4591988043624866 1107 40-41 0.023959332619206464 1107 42-43 0.3628670842876005 1107 44-45 0.5098916223790155 1107 46-47 0.40170287927729476 1107 48-49 -0.36171945746584555 1107 50-51 -0.3458364343223792 1107 52-53 0.201960972812131 1107 54-55 0.04672355769367442 1107 56-57 0.5455396711383287 1107 58-59 -0.00820380717321001 1107 60-61 -0.29729217174789113 1107 62-63 -0.1803378394214903 1107 64-65 0.17574653731116996 1107 66-67 -0.4381794814052711 1107 68-69 -0.24603818665698896 1107 70-71 -0.48880860853859076 1107 72-73 -0.39828724483120226 1107 74-75 -0.18602391684605024 1107 76-77 -0.07004324049541921 1107 78-79 -0.2579566813588521 1107 80-81 -0.18846288811982603 1107 82-83 -0.005681954788538945 1107 84-85 -0.10761236061324553 1107 86-87 0.032728398836727735 1107 88-89 -0.09061316146611986 1107 90-91 -0.4751045391665052 1107 92-93 -1.1975009663111997 1107 94-95 -0.8085807460298362 1107 96-97 -0.9557963185551692 1107 98-99 -0.332846423812974 1107 100-101 -0.43678231008762225 1108 1 -0.22779570902199353 1108 2 -0.10136580918238636 1108 3 -0.19357920342471857 1108 4 0.2659553843409128 1108 5 0.3637933016250656 1108 6 -0.17610585021133573 1108 7 -0.41116667563143494 1108 8 -0.06402843982500883 1108 9 -0.03053313999991758 1108 10-11 -0.371988404096669 1108 12-13 -0.06448880027461001 1108 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-0.10619606648621982 2316 60-61 0.34380603821823996 2316 62-63 0.5805716912993617 2316 64-65 0.627584950470343 2316 66-67 1.1510803154016926 2316 68-69 0.8993657804450947 2316 70-71 0.775366524504463 2316 72-73 -0.7518915022090482 2316 74-75 -0.5509973082345354 2316 76-77 -0.4030862980667784 2316 78-79 -1.088653343187591 2316 80-81 -0.6247231687197292 2316 82-83 -0.7071817128920692 2316 84-85 -0.7567173436804921 2316 86-87 -1.4826078372542248 2316 88-89 -1.3643432050074829 2316 90-91 -0.9410213267814065 2316 92-93 -1.0526291714394063 2316 94-95 -1.1004046454009035 2316 96-97 -1.6589167829963856 2316 98-99 -2.11001139720436 2316 100-101 -2.2897334470010264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 499.0 3 125.0 4 127.0 5 115.0 6 139.0 7 120.0 8 152.0 9 212.0 10 269.0 11 402.0 12 562.0 13 818.0 14 901.0 15 1244.0 16 1754.0 17 2375.0 18 2119.0 19 1165.0 20 1118.0 21 1190.0 22 1287.0 23 1379.0 24 1438.0 25 1633.0 26 1740.0 27 2023.0 28 2171.0 29 2623.0 30 3101.0 31 4024.0 32 4993.0 33 6434.0 34 9017.0 35 13751.0 36 18776.0 37 31872.0 38 38774.0 39 8032.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.394238761309936 25.010092854259184 17.952812937851768 20.642855446579116 2 13.37342174153068 24.77784175565858 41.327666344139 20.52107015867174 3 14.483651129105027 31.382233514591697 33.58582860399402 20.548286752309263 4 9.889627083222011 22.031835372768345 43.01515772224498 25.06337982176466 5 9.195654885284457 40.35920248495899 38.33393715144352 12.11120547831303 6 22.38526770867975 44.373841987742885 20.180293600646113 13.060596702931257 7 21.169395533286632 36.67109934340976 27.113733778184105 15.045771345119505 8 20.745887523012055 36.93746659540353 24.195023457449967 18.12162242413445 9 23.079024089017143 18.98976944132718 25.20113765237476 32.730068817280916 10-11 19.281486187222065 30.46707553835102 31.73851606889468 18.512922205532238 12-13 21.342450054480622 29.774922287176715 31.029906447714932 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15.8839590443686 32.473067220655885 34.4092595340555 17.233714200920016 40-41 15.867932927734085 31.793441163377356 35.272295592817926 17.066330316070633 42-43 16.22881733194836 31.736756195281203 33.54117821635258 18.493248256417864 44-45 16.271650224960528 31.87969657039068 33.55829386136733 18.290359343281455 46-47 16.93634070336845 31.716278379581542 33.579759608250484 17.767621308799527 48-49 16.87555089939427 31.847846409990833 33.35015091304619 17.92645177756871 50-51 16.70040065291586 31.700549042884703 32.92090814660929 18.67814215759015 52-53 16.708463298600083 32.35949654101956 32.92723824673764 18.00480191364272 54-55 17.394685138688555 31.565076482005804 32.630541754960795 18.409696624344843 56-57 17.383936441419916 32.24612626836702 31.711030452679783 18.658906837533277 58-59 16.858337611923467 31.36231299098677 32.90943139762991 18.869917999459858 60-61 17.420609010506322 32.390633347183474 31.938030509883063 18.250727132427137 62-63 16.810805356379696 32.5030272092692 32.85323377178403 17.832933662567076 64-65 17.34397397810338 31.398409838936093 32.73511857947986 18.522497603480666 66-67 16.96565315983806 31.891331964050384 32.114829809210605 19.02818506690095 68-69 16.776585261608233 31.576416673592227 32.98923173728763 18.6577663275119 70-71 17.681824523490768 31.003303876489046 32.81613170385631 18.498739896163883 72-73 17.064555673294688 30.88277634808832 32.655393392163326 19.397274586453666 74-75 16.961609013897217 31.802720078835982 31.886127123020934 19.349543784245864 76-77 16.693680535765175 31.247922010069345 32.80878930369526 19.24960815047022 78-79 16.656475588905774 30.645065539513677 32.430186170212764 20.26827270136778 80-81 17.24952937473648 31.73291051290731 31.564554346083266 19.453005766272945 82-83 17.664260881698915 30.946747143192205 31.819301896819436 19.569690078289447 84-85 16.900170972644375 30.613898651215806 31.58927384118541 20.89665653495441 86-87 17.329126360562896 31.11685628715854 31.63896812416704 19.915049228111524 88-89 17.681768533029395 29.988246188915802 32.072469962482785 20.257515315572018 90-91 17.583184167327154 29.19319743642263 32.7829775612768 20.44064083497342 92-93 18.379803353441314 30.10437942336009 31.685508003609936 19.830309219588656 94-95 17.696871837035147 29.282264838664673 32.32672517935217 20.694138144948013 96-97 17.907860365708856 29.52386606506768 31.92412728568036 20.6441462835431 98-99 18.146624153143684 30.27336907794344 31.33739468105951 20.242612087853367 100-101 18.055984418422366 30.08776513622004 31.11149378874611 20.744756656611482 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1828.0 1 1136.0 2 410.5 3 349.0 4 291.5 5 264.0 6 372.5 7 678.5 8 923.0 9 915.0 10 712.5 11 534.0 12 530.5 13 540.5 14 528.5 15 512.0 16 485.0 17 510.0 18 524.0 19 520.0 20 584.5 21 647.0 22 689.5 23 781.0 24 903.5 25 1051.0 26 1443.5 27 1878.0 28 1928.5 29 2226.5 30 3118.0 31 3988.0 32 5278.5 33 6949.0 34 8049.0 35 9042.0 36 10866.0 37 12161.0 38 11596.5 39 10338.0 40 8988.0 41 7685.0 42 6201.0 43 5626.0 44 5710.5 45 5293.0 46 4299.0 47 3615.0 48 3885.5 49 3259.5 50 2097.0 51 1365.0 52 989.0 53 772.0 54 594.5 55 520.0 56 439.0 57 352.5 58 325.0 59 313.0 60 271.0 61 225.0 62 184.5 63 116.0 64 50.5 65 32.5 66 24.5 67 19.5 68 10.5 69 8.5 70 8.0 71 5.5 72 3.0 73 4.0 74 4.5 75 3.5 76 2.0 77 1.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02314883513874462 2 0.008309838254933966 3 0.013651877133105802 4 0.027303754266211604 5 0.060543107285947466 6 0.04926546965425137 7 0.01721323638522036 8 0.05045258940495623 9 0.03442647277044072 10-11 0.02611663451550675 12-13 0.03947173171093634 14-15 0.022852055201068408 16-17 0.022852055201068408 18-19 0.01958747588663006 20-21 0.015135776821486866 22-23 0.0074194984419053275 24-25 0.0017806796260572786 26-27 0.0 28-29 0.0023742395014097048 30-31 0.0 32-33 2.967799376762131E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 5.935598753524262E-4 46-47 0.0 48-49 2.967799376762131E-4 50-51 0.0 52-53 2.967799376762131E-4 54-55 0.0023742395014097048 56-57 2.967799376762131E-4 58-59 2.967799376762131E-4 60-61 0.0029677993767621307 62-63 0.0017806796260572786 64-65 8.903398130286393E-4 66-67 0.010090517880991244 68-69 0.010090517880991244 70-71 0.021664935450363555 72-73 0.013948657070782014 74-75 0.014245437008458229 76-77 0.02552307464015433 78-79 0.016026116634515506 80-81 0.048671909778898945 82-83 0.03650393233417421 84-85 0.016026116634515506 86-87 0.01454221694613444 88-89 0.011277637631696097 90-91 0.022852055201068408 92-93 0.030271553642973733 94-95 0.012761537320077164 96-97 0.020774595637334914 98-99 0.03442647277044072 100-101 0.04214275115002226 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 168475.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.30894791512094 #Duplication Level Percentage of deduplicated Percentage of total 1 75.64577091044397 22.927437305238165 2 8.773475902316747 5.318296483157738 3 3.5563911246891093 3.2337142009200175 4 2.0876172571137617 2.530939308502745 5 1.3728139748937587 2.0804273631102537 6 1.047725358870415 1.905327199881288 7 0.8127215400583593 1.7242914378987981 8 0.6305935804790161 1.52901023890785 9 0.5463838787380295 1.490428847009942 >10 4.702034741397881 29.742691794034727 >50 0.6129682940681119 12.962160557946284 >100 0.20171161114701444 9.410298263837365 >500 0.0 0.0 >1k 0.009791825783835653 5.144976999554831 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2421 1.4370084582282239 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1870 1.109956966909037 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1824 1.0826532126428252 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1549 0.9194242469209082 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.5959341148538359 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 375 0.22258495325715982 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 349 0.20715239649799674 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 319 0.18934560023742394 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.17806796260572785 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 296 0.17569372310431813 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 295 0.1751001632289657 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 264 0.1566998070930405 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 258 0.15313844784092595 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 241 0.1430479299599347 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 239 0.14186081020922986 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 238 0.14126725033387744 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 230 0.13651877133105803 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 224 0.13295741207894346 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 210 0.12464757382400951 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 209 0.12405401394865707 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 201 0.11930553494583766 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 199 0.1181184151951328 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 196 0.11633773556907552 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 196 0.11633773556907552 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 191 0.11336993619231339 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 191 0.11336993619231339 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 190 0.11277637631696097 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189 0.11218281644160855 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 181 0.10743433743878913 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 179 0.10624721768808429 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 171 0.10149873868526488 No Hit GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT 169 0.10031161893456003 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.935598753524262E-4 0.0 14-15 0.0 0.0 0.0 0.0011871197507048524 0.0 16-17 0.0 0.0 0.0 0.0011871197507048524 0.0 18-19 0.0 0.0 0.0 0.0011871197507048524 0.0 20-21 0.0 0.0 0.0 0.0011871197507048524 0.0 22-23 0.0 0.0 0.0 0.002671019439085918 0.0 24-25 0.0 0.0 0.0 0.00385813918979077 0.0 26-27 0.0 0.0 0.0 0.00979373794331503 0.0 28-29 0.0 0.0 0.0 0.017806796260572785 0.0 30-31 0.0 0.0 0.0 0.03561359252114557 0.0 32-33 0.0 0.0 0.0 0.0522332690310135 0.0 34-35 0.0 0.0 0.0 0.0635109066627096 0.0 36-37 0.0 0.0 0.0 0.08102092298560618 0.0 38-39 0.0 0.0 0.0 0.1109956966909037 0.0 40-41 0.0 0.0 0.0 0.13355097195429588 0.0 42-43 0.0 0.0 0.0 0.19142305980115742 0.0 44-45 0.0 0.0 0.0 0.2570114260276005 0.0 46-47 0.0 0.0 0.0 0.31696097343819557 0.0 48-49 0.0 0.0 0.0 0.39174951773260125 0.0 50-51 0.0 0.0 0.0 0.48553197803828463 0.0 52-53 0.0 0.0 0.0 0.6570707820151358 0.0 54-55 0.0 0.0 0.0 0.8122866894197952 0.0 56-57 0.0 0.0 0.0 1.0672206558836623 0.0 58-59 0.0 0.0 0.0 1.3951624870158779 0.0 60-61 0.0 0.0 0.0 1.6922392046297672 0.0 62-63 0.0 0.0 0.0 2.0557946282831283 0.0 64-65 0.0 0.0 0.0 2.358213384775189 0.0 66-67 0.0 0.0 0.0 2.8200029677993768 0.0 68-69 0.0 0.0 0.0 3.3545036355542366 0.0 70-71 0.0 0.0 0.0 3.800860661819261 0.0 72-73 0.0 0.0 0.0 4.430924469505861 0.0 74-75 0.0 0.0 0.0 5.053568778750556 0.0 76-77 0.0 0.0 0.0 5.5198100608398875 0.0 78-79 0.0 0.0 0.0 6.018400356135925 0.0 80-81 0.0 0.0 0.0 6.791215313844784 0.0 82-83 0.0 0.0 0.0 7.395162487015877 0.0 84-85 0.0 0.0 0.0 8.310135034871642 0.0 86-87 0.0 0.0 0.0 9.411782163525746 0.0 88-89 0.0 0.0 0.0 10.665083840332393 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTTAT 105 4.6158675E-8 36.19172 7 CGCAGAA 120 1.4924444E-7 31.667755 9 CGTAAAT 30 0.0039520985 31.658354 54-55 GGTATCA 1005 0.0 30.249796 1 GTATCAA 1385 0.0 26.751822 1 GTATAGT 90 8.9139555E-4 26.389795 1 TATTCCC 150 1.0508284E-6 25.334204 5 ATACTTA 200 2.0268999E-8 23.750816 6 CTTACTA 100 0.0016474816 23.750816 9 GGGATGA 50 0.0016537531 23.743767 20-21 AAAGTCA 185 2.5141708E-7 23.108902 9 CTATTCC 185 2.5141708E-7 23.108902 4 GTATAGG 185 2.5141708E-7 23.108902 1 TATAGGA 185 2.5141708E-7 23.108902 2 CAACGCA 1585 0.0 23.076504 5 TCAACGC 1585 0.0 23.076504 4 ATCAACG 1595 0.0 22.931824 3 AACGCAG 1605 0.0 22.788946 6 GGGGTGA 105 0.002188065 22.619825 9 CAATACT 210 3.4009645E-8 22.619825 4 >>END_MODULE