##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937117_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 168475 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00047484790028 31.0 31.0 34.0 30.0 34.0 2 32.15826680516397 33.0 31.0 34.0 30.0 34.0 3 32.21439382697729 34.0 31.0 34.0 30.0 34.0 4 35.93611515061582 37.0 35.0 37.0 35.0 37.0 5 35.83288915269328 37.0 35.0 37.0 35.0 37.0 6 35.875815402878764 37.0 35.0 37.0 35.0 37.0 7 35.82442498887075 37.0 35.0 37.0 35.0 37.0 8 35.80440421427512 37.0 35.0 37.0 33.0 37.0 9 37.44697432853539 39.0 37.0 39.0 34.0 39.0 10-11 37.3646357026265 39.0 37.0 39.0 34.0 39.0 12-13 37.31778898946432 39.0 37.0 39.0 34.0 39.0 14-15 38.41683632586437 40.0 38.0 41.0 33.5 41.0 16-17 38.31597269624574 40.0 38.0 41.0 33.5 41.0 18-19 38.4272414304793 40.0 38.0 41.0 34.0 41.0 20-21 38.466104763318 40.0 38.0 41.0 34.0 41.0 22-23 38.34654399762576 40.0 38.0 41.0 34.0 41.0 24-25 38.22633031607063 40.0 37.0 41.0 34.0 41.0 26-27 38.01712123460454 40.0 37.0 41.0 33.0 41.0 28-29 37.88395607656923 40.0 37.0 41.0 33.0 41.0 30-31 37.77357471434931 40.0 36.5 41.0 33.0 41.0 32-33 37.49389226888262 39.5 35.5 41.0 33.0 41.0 34-35 37.296266508384036 39.0 35.0 41.0 33.0 41.0 36-37 37.10525003709749 39.0 35.0 41.0 32.5 41.0 38-39 36.92638076866004 39.0 35.0 41.0 32.0 41.0 40-41 36.72876984715833 39.0 35.0 41.0 31.0 41.0 42-43 36.416480189939165 39.0 35.0 41.0 31.0 41.0 44-45 36.07842409853094 39.0 35.0 41.0 30.0 41.0 46-47 35.617510016322896 39.0 35.0 40.0 28.0 41.0 48-49 35.151868229707674 39.0 35.0 40.0 26.5 41.0 50-51 34.82417866152248 38.5 35.0 40.0 24.0 41.0 52-53 34.60656180442202 38.0 35.0 40.0 23.5 41.0 54-55 34.300145422169464 38.0 34.0 40.0 22.5 41.0 56-57 34.00209823415938 38.0 34.0 40.0 21.0 41.0 58-59 33.669111144086656 38.0 33.0 40.0 19.0 41.0 60-61 33.36998367710343 38.0 33.0 40.0 16.5 41.0 62-63 32.98305089775931 37.0 33.0 40.0 12.5 41.0 64-65 32.58060543107286 37.0 32.0 39.5 8.0 40.5 66-67 32.177578275708555 36.0 32.0 39.0 4.5 40.0 68-69 31.831975070485235 36.0 31.0 39.0 2.0 40.0 70-71 31.380106840777565 35.5 31.0 38.5 2.0 40.0 72-73 30.814672800118714 35.0 30.5 38.0 2.0 40.0 74-75 30.286650838403325 35.0 29.5 37.0 2.0 39.0 76-77 28.81525152099718 33.0 28.5 35.5 2.0 38.5 78-79 29.50179551862294 34.0 29.0 36.0 2.0 39.0 80-81 29.125487461047634 34.0 29.0 36.0 2.0 38.0 82-83 28.795028936043924 34.0 29.0 35.5 2.0 37.0 84-85 28.402415788692686 34.0 29.0 35.0 2.0 37.0 86-87 28.03480931889004 34.0 28.5 35.0 2.0 36.0 88-89 27.60599198694168 34.0 27.0 35.0 2.0 36.0 90-91 27.139964386407478 33.0 26.0 35.0 2.0 35.5 92-93 26.61650690013355 33.0 25.5 35.0 2.0 35.0 94-95 26.00000890339813 32.5 24.5 34.5 2.0 35.0 96-97 25.293746846713162 32.0 21.0 34.0 2.0 35.0 98-99 24.545202552307465 32.0 10.0 34.0 2.0 35.0 100-101 21.364442795667014 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24535638315839847 1101 2 0.3145111011069446 1101 3 0.29410062997934006 1101 4 0.20679006341100603 1101 5 0.18300697559426027 1101 6 0.2779375699193949 1101 7 0.3549698625415587 1101 8 0.2608822885336153 1101 9 0.47919054694410335 1101 10-11 0.20062925589540015 1101 12-13 0.3437608814888691 1101 14-15 0.44706382868001526 1101 16-17 0.32790502785262987 1101 18-19 0.25131561277607517 1101 20-21 0.18510256125711777 1101 22-23 0.5289099224759326 1101 24-25 0.580640804326805 1101 26-27 0.37983101905957994 1101 28-29 0.7355733342475617 1101 30-31 0.6856338010476577 1101 32-33 0.5386146537349461 1101 34-35 0.679533228648161 1101 36-37 0.6315644838927241 1101 38-39 0.6109656832057695 1101 40-41 0.8749272581806053 1101 42-43 0.5241422606345836 1101 44-45 0.7719120110797633 1101 46-47 0.9563935597231037 1101 48-49 0.9536491814079326 1101 50-51 0.901249403844183 1101 52-53 0.9224959952762859 1101 54-55 0.4946964674076355 1101 56-57 1.0500346553935458 1101 58-59 0.6986450253725067 1101 60-61 0.781217978030142 1101 62-63 0.8406614804156476 1101 64-65 0.6948646850460669 1101 66-67 0.7067752201824966 1101 68-69 0.982033334461434 1101 70-71 0.7517155656731553 1101 72-73 0.6392843553955814 1101 74-75 1.2031896519749026 1101 76-77 1.0272527624655687 1101 78-79 0.9706378481605213 1101 80-81 1.1791865479114279 1101 82-83 1.0232422603982592 1101 84-85 0.8140449812766768 1101 86-87 0.6226513747220714 1101 88-89 0.5519925305623126 1101 90-91 1.1863225803703585 1101 92-93 1.3738530206051855 1101 94-95 1.2243956802061575 1101 96-97 1.2481168704970855 1101 98-99 0.943826354725612 1101 100-101 1.2554508370843216 1102 1 0.39233194784415915 1102 2 0.3813592797619165 1102 3 0.25795877541172274 1102 4 0.21915515806590946 1102 5 0.24950130416372218 1102 6 0.3847806374064717 1102 7 0.04024969898419073 1102 8 0.3185008774581064 1102 9 0.3092737017141971 1102 10-11 0.3255285069127183 1102 12-13 0.2249213131859733 1102 14-15 0.45583636205004296 1102 16-17 0.5194313012214806 1102 18-19 0.3136128861645844 1102 20-21 0.4341982777052493 1102 22-23 0.4196931106652073 1102 24-25 0.6828494186105516 1102 26-27 0.4483304981136129 1102 28-29 0.7542094387512819 1102 30-31 0.9946825340677634 1102 32-33 0.8578218329600205 1102 34-35 1.0584231222965457 1102 36-37 1.0061786890078253 1102 38-39 1.2304146599892718 1102 40-41 1.2307186045801757 1102 42-43 1.1939264120824191 1102 44-45 1.29007631546947 1102 46-47 1.6212287245582715 1102 48-49 1.8221742163014838 1102 50-51 1.585472604614786 1102 52-53 1.4887839112958687 1102 54-55 1.6853676030698281 1102 56-57 1.6995952915373707 1102 58-59 1.6993035796983236 1102 60-61 1.6594198298455893 1102 62-63 1.9366384015267428 1102 64-65 1.846351838322125 1102 66-67 2.0590772115721414 1102 68-69 1.7261554913742856 1102 70-71 1.7259139969377735 1102 72-73 1.816592735745104 1102 74-75 2.2377146082351054 1102 76-77 2.3048284962130374 1102 78-79 2.156399313886208 1102 80-81 2.651197989064194 1102 82-83 2.711662516349822 1102 84-85 2.9020848483863126 1102 86-87 2.185145231491422 1102 88-89 2.327548370071643 1102 90-91 2.736893655091702 1102 92-93 2.6898116594618564 1102 94-95 2.4456650190962463 1102 96-97 2.310296193857088 1102 98-99 2.3829149941720367 1102 100-101 1.9489920901067883 1103 1 0.19395870921430358 1103 2 0.3914749860283848 1103 3 0.42278865283181943 1103 4 0.14068110605536788 1103 5 0.2992893669429648 1103 6 0.10689084476126709 1103 7 0.07751628163970281 1103 8 0.12001307816838391 1103 9 0.3458300082785257 1103 10-11 0.4382848363891796 1103 12-13 0.2737241542903419 1103 14-15 0.35691896028580317 1103 16-17 0.45201235288833885 1103 18-19 0.45570247260059915 1103 20-21 0.32102380182026735 1103 22-23 0.6379999041123341 1103 24-25 0.23140595429619992 1103 26-27 0.43077980502162205 1103 28-29 0.45502854746934673 1103 30-31 0.5885821789297196 1103 32-33 0.7436850475321322 1103 34-35 0.9827672625187986 1103 36-37 0.7165063324950509 1103 38-39 0.7060075114041027 1103 40-41 0.9030235650567562 1103 42-43 0.5804368943827996 1103 44-45 0.7771252328712421 1103 46-47 0.7175341432774829 1103 48-49 0.5256855005264853 1103 50-51 0.7288968227382853 1103 52-53 1.0959095843397435 1103 54-55 1.06238673470002 1103 56-57 0.8268149043223403 1103 58-59 1.2278635522037646 1103 60-61 1.142705429570647 1103 62-63 1.6961297521969172 1103 64-65 1.3792250196716545 1103 66-67 1.2150286378523596 1103 68-69 1.0301765705357013 1103 70-71 1.112080797258546 1103 72-73 1.6618613804924998 1103 74-75 1.679500608922467 1103 76-77 1.5005439275339185 1103 78-79 1.6799930817863107 1103 80-81 1.980502605858785 1103 82-83 1.921405900471715 1103 84-85 1.6812863605870199 1103 86-87 1.3335471102862435 1103 88-89 1.4257325836127102 1103 90-91 1.4495239678423033 1103 92-93 1.5447263117702548 1103 94-95 1.2839855598225611 1103 96-97 1.598943232464034 1103 98-99 1.9110167240379958 1103 100-101 1.7592051729341485 1104 1 0.21957447276110642 1104 2 0.06272442624334218 1104 3 0.22413213693392464 1104 4 0.23173947297580355 1104 5 0.20530519020017124 1104 6 -0.024964656059751178 1104 7 0.06209606966776704 1104 8 0.1963826855724733 1104 9 0.26759445073262356 1104 10-11 0.11043739647276851 1104 12-13 0.16773818623540393 1104 14-15 0.2086804172196821 1104 16-17 0.25787890013419457 1104 18-19 0.33384846453969175 1104 20-21 0.18845177614479525 1104 22-23 -0.16900114081974493 1104 24-25 0.08021174620544258 1104 26-27 -0.07381789612780665 1104 28-29 -0.16998264823150322 1104 30-31 0.24641916952533194 1104 32-33 0.2759734487860541 1104 34-35 0.17203431730309404 1104 36-37 0.675462893175748 1104 38-39 0.5080749841612331 1104 40-41 0.41757051236231746 1104 42-43 0.7054033072802426 1104 44-45 0.570907886998711 1104 46-47 0.3679819713115151 1104 48-49 0.3248420910623864 1104 50-51 0.18740555538494874 1104 52-53 0.4546049120005904 1104 54-55 0.17650824537325605 1104 56-57 0.5317708678989916 1104 58-59 0.4203848240353807 1104 60-61 0.6433622440205582 1104 62-63 0.8162820137284896 1104 64-65 1.0186562780684305 1104 66-67 0.7073707784703593 1104 68-69 0.9188465212746166 1104 70-71 0.32870268112180767 1104 72-73 0.20106872487225758 1104 74-75 0.6274256723647689 1104 76-77 0.7770926661516242 1104 78-79 0.9001826623207201 1104 80-81 1.289877139371253 1104 82-83 1.3400728656904022 1104 84-85 1.1452138124455082 1104 86-87 1.461043751575989 1104 88-89 1.0420857706555502 1104 90-91 1.3909256695888317 1104 92-93 1.0905465834525963 1104 94-95 1.3228421055756598 1104 96-97 0.7849262149987375 1104 98-99 1.3130468777922921 1104 100-101 1.8009502095067695 1105 1 -0.007026512460569734 1105 2 0.04615478898405456 1105 3 0.03559653460521872 1105 4 0.01752839669575934 1105 5 0.05199872654887372 1105 6 0.05713715017998311 1105 7 0.03761480380719462 1105 8 -0.09082436025526164 1105 9 -0.0024458537904408217 1105 10-11 -0.16393191237929727 1105 12-13 -0.2853891462022702 1105 14-15 -0.253179561881673 1105 16-17 -0.10956400179146897 1105 18-19 -0.005381271241766683 1105 20-21 -0.12577422773637892 1105 22-23 0.08282749031923231 1105 24-25 0.1823911759710768 1105 26-27 0.0977748789132491 1105 28-29 0.16364923712452395 1105 30-31 0.2706018833632129 1105 32-33 0.1579707618178503 1105 34-35 0.12217612951386769 1105 36-37 0.043353623135452324 1105 38-39 0.4737415015518849 1105 40-41 0.3618905601306466 1105 42-43 0.6348703919197405 1105 44-45 0.6458444446938927 1105 46-47 0.41433217283412915 1105 48-49 0.23430619018164833 1105 50-51 0.043428842442715165 1105 52-53 0.1565007173446702 1105 54-55 0.17815028149805556 1105 56-57 -0.2298353419830761 1105 58-59 0.05742020245006785 1105 60-61 0.0850699615903423 1105 62-63 0.2961297521969186 1105 64-65 -0.08456808377662028 1105 66-67 -0.3864491946599564 1105 68-69 -0.31760668069582465 1105 70-71 0.1468098612979567 1105 72-73 0.23599931152698161 1105 74-75 0.1748208059667995 1105 76-77 0.1197557501940203 1105 78-79 -0.04734681969152277 1105 80-81 -0.3298422217274215 1105 82-83 0.08175072805791928 1105 84-85 -0.0480239842405652 1105 86-87 -0.37729032813740204 1105 88-89 -0.573528500131129 1105 90-91 -0.29357948043356075 1105 92-93 -0.484091422219894 1105 94-95 -0.5899060657932012 1105 96-97 0.038844710296540796 1105 98-99 -0.42691431044476147 1105 100-101 -0.7774943344550174 1106 1 -0.2361318321072119 1106 2 0.06134591648494592 1106 3 0.057695221893652615 1106 4 -0.17313126150386893 1106 5 -0.05852223005183532 1106 6 0.08171265173487541 1106 7 0.060814297436273534 1106 8 -0.062148355081468765 1106 9 -0.09125340532193604 1106 10-11 -0.11945933621725402 1106 12-13 0.0050861269353035254 1106 14-15 -0.13517312216458066 1106 16-17 -0.27019916705001634 1106 18-19 -0.4971389837661846 1106 20-21 -8.966379328967378E-5 1106 22-23 -0.11068050428681886 1106 24-25 0.05838534169746623 1106 26-27 0.09564206662825114 1106 28-29 0.22423486609775267 1106 30-31 0.2159468485502174 1106 32-33 0.10685206737417019 1106 34-35 0.6212954844413545 1106 36-37 0.5109486364827234 1106 38-39 0.4289799637254319 1106 40-41 0.3270362793699917 1106 42-43 0.02492735864787221 1106 44-45 0.21277347020507875 1106 46-47 0.1328009512142927 1106 48-49 0.5942561034931515 1106 50-51 0.6128209221410899 1106 52-53 0.2110319114443513 1106 54-55 -0.30247552690660484 1106 56-57 0.4624342454897459 1106 58-59 0.5062877479271322 1106 60-61 0.32785280001949246 1106 62-63 0.37280091382280744 1106 64-65 0.34993393895502933 1106 66-67 0.8689103562110567 1106 68-69 -0.3006267428913816 1106 70-71 -0.14098219638429654 1106 72-73 0.1429536555840052 1106 74-75 -0.40683150435050663 1106 76-77 -0.9704828495803142 1106 78-79 -0.7782134294831913 1106 80-81 -0.26155094836968473 1106 82-83 -0.22194798124664672 1106 84-85 -0.14964216956131438 1106 86-87 -0.20644518406936996 1106 88-89 -0.5054849082000423 1106 90-91 -0.46175516912552794 1106 92-93 -0.19394639098451094 1106 94-95 -0.06841282199211562 1106 96-97 0.5547513619188571 1106 98-99 0.8152355643385185 1106 100-101 1.2937553559432011 1107 1 0.18363556889767452 1107 2 0.07750838442353114 1107 3 0.1514375359374327 1107 4 0.2256952027805923 1107 5 0.03353356559835419 1107 6 0.18437945894473984 1107 7 -0.018276388063178217 1107 8 -0.10501403091858208 1107 9 -0.1364731797501051 1107 10-11 -0.07423956178909208 1107 12-13 -0.11773106149799162 1107 14-15 -0.21507193106096878 1107 16-17 -0.19687075272866394 1107 18-19 -0.11363172822351686 1107 20-21 -0.44917788978676043 1107 22-23 -0.6661423643760642 1107 24-25 -0.3848703415181589 1107 26-27 -0.30652229864352876 1107 28-29 -0.04411480538252732 1107 30-31 -0.18497019582629548 1107 32-33 -0.3809733779720972 1107 34-35 -0.4530167322762608 1107 36-37 -0.6980566690230532 1107 38-39 -0.2281824690773533 1107 40-41 -0.7692709480640474 1107 42-43 -1.0773705226633936 1107 44-45 -0.960252776237418 1107 46-47 -0.6227065854147824 1107 48-49 -0.5474114415685065 1107 50-51 -0.8086329972573836 1107 52-53 -0.021830602170780367 1107 54-55 -0.2367891490562144 1107 56-57 -0.35449066930594597 1107 58-59 -0.5848018132267256 1107 60-61 -1.0448677649641631 1107 62-63 -1.450237630149644 1107 64-65 -1.1495214561470384 1107 66-67 -0.824810876352803 1107 68-69 -0.7940914841162154 1107 70-71 -0.7590209160357482 1107 72-73 -0.9528968064517613 1107 74-75 -0.8773525682625269 1107 76-77 -0.8369316742878112 1107 78-79 -1.113988700907246 1107 80-81 -0.35608381791046284 1107 82-83 -0.3328014298254054 1107 84-85 -0.28658394954093325 1107 86-87 -0.40308268802214897 1107 88-89 -0.9430485918373002 1107 90-91 -0.7350238525871049 1107 92-93 -0.6567375789259096 1107 94-95 -0.6157758802121371 1107 96-97 -0.8486603242481792 1107 98-99 -1.0875085416310561 1107 100-101 -0.9071687155932899 1108 1 -0.11120213318328709 1108 2 -0.18795107275430212 1108 3 -0.24191859086177203 1108 4 -0.16225802840424564 1108 5 -0.253453053792974 1108 6 -0.00898510987886425 1108 7 -0.02887112868197761 1108 8 -0.016668245599262832 1108 9 -0.039474319423398185 1108 10-11 -0.27190956305539515 1108 12-13 -0.015329305556925021 1108 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-1.7628333699273568 2316 94-95 -2.2034149706814183 2316 96-97 -1.2323565022839809 2316 98-99 -1.4348718701264573 2316 100-101 -1.9838982753417156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 18.0 6 27.0 7 41.0 8 88.0 9 130.0 10 263.0 11 418.0 12 589.0 13 788.0 14 1143.0 15 1566.0 16 2045.0 17 2663.0 18 2461.0 19 1506.0 20 1282.0 21 1359.0 22 1413.0 23 1603.0 24 1754.0 25 1858.0 26 2034.0 27 2420.0 28 2907.0 29 3637.0 30 4315.0 31 5163.0 32 6360.0 33 8081.0 34 11016.0 35 15076.0 36 21427.0 37 34648.0 38 26780.0 39 1592.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.37524044741029 25.177515495499776 18.912227789783657 19.535016267306275 2 14.029287287275405 25.71986537582582 40.503594133045254 19.74725320385353 3 15.536429737349755 31.1066923875946 33.95934114853836 19.397536726517288 4 10.80635109066627 22.647870603947172 42.352277786021666 24.193500519364893 5 9.80560914082208 40.88024929514765 37.99614186081021 11.317999703220062 6 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35.98066072875456 16.145442241883355 28-29 16.498045698310428 30.43843430311412 36.55657087571116 16.50694912286429 30-31 17.528395347525812 30.693389604646537 35.380588177682014 16.397626870145636 32-33 16.74838395650187 31.611532228863815 35.37220939412467 16.267874420509653 34-35 17.05891081762873 31.04644606024633 35.45214423504971 16.44249888707523 36-37 17.565417675832265 30.636189865602887 34.84292634278387 16.955466115780975 38-39 16.422588553755556 31.502401206242542 35.52835441685415 16.546655823147745 40-41 16.192160784930568 31.17582290389883 36.0472121728343 16.584804138336295 42-43 16.602166493545038 30.877281495770887 34.740465944502155 17.780086066181926 44-45 16.968096156699808 31.244695058614035 34.42380175100163 17.36340703368452 46-47 17.48959041279244 31.083339021632355 34.50047336540545 16.92659720016976 48-49 17.57223203288468 31.568996987549525 33.703533322450916 17.155237657114874 50-51 17.293308956216165 31.27582154050717 33.416041409440595 18.014828093836073 52-53 17.314735123905624 31.83914527377949 33.622792699213534 17.22332690310135 54-55 18.27819443991013 31.19930432098582 32.99311147187753 17.529389767226515 56-57 18.129744938237895 31.807632264689644 32.075337302411725 17.98728549466074 58-59 17.47558985012613 31.20225552752634 33.33818073898204 17.983973883365483 60-61 17.985374511049248 32.33069987475738 32.063891543452065 17.620034070741305 62-63 17.595206083216837 32.002184449450475 33.38646056860981 17.016148898722868 64-65 17.73824009496958 31.647128654102985 32.70663303160706 17.907998219320373 66-67 17.607481406295445 32.028064177217445 31.918846567064957 18.445607849422156 68-69 17.15106530107109 31.423636358240316 32.75767405556363 18.66762428512496 70-71 17.95108458550107 31.434133949054605 32.899044068983734 17.715737396460593 72-73 17.657219171983975 30.82979670574269 32.32586437156848 19.187119750704852 74-75 17.39634960676658 31.784834545184747 31.780679626057278 19.038136221991394 76-77 17.201958747588662 31.352129396052824 32.339219468763915 19.1066923875946 78-79 17.317959904435444 30.65010610040214 32.22988915104839 19.802044844114025 80-81 17.959714254339755 31.908661108852236 30.697773807439237 19.433850829368765 82-83 18.15777601006803 30.83482731190711 31.348914243651087 19.658482434373774 84-85 16.93715459364482 30.970669769264102 31.203364576964397 20.888811060126677 86-87 17.11501819325811 31.163596863517917 31.257679454380334 20.463705488843644 88-89 17.50942276302122 30.6250185487461 31.357768214868674 20.507790473364 90-91 17.004701022116173 29.72832603250353 32.598205064282915 20.66876788109738 92-93 17.64742543404066 30.771627837958153 31.31265766434189 20.2682890636593 94-95 17.33461937972993 29.77830538655587 31.612405401394867 21.274669832319333 96-97 17.438468090663296 30.014928518218433 31.229985249555558 21.316618141562714 98-99 17.016774300774014 30.898309511372812 30.87159884135049 21.213317346502684 100-101 16.633625166938714 30.81673838848494 30.643122124944355 21.90651431963199 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2104.0 1 1341.0 2 500.5 3 385.0 4 313.0 5 291.5 6 365.5 7 561.5 8 735.5 9 747.0 10 608.0 11 480.5 12 505.5 13 537.5 14 547.0 15 559.0 16 604.0 17 630.0 18 592.0 19 604.0 20 651.0 21 704.5 22 749.5 23 818.0 24 913.0 25 1115.5 26 1540.0 27 1919.0 28 1979.5 29 2279.0 30 3200.0 31 3885.5 32 4895.0 33 6544.0 34 7688.0 35 8927.5 36 10841.5 37 11938.5 38 11467.0 39 10318.0 40 9060.5 41 7699.5 42 6054.0 43 5545.5 44 5699.0 45 5407.5 46 4536.5 47 3928.5 48 4155.5 49 3431.0 50 2238.0 51 1441.5 52 944.5 53 715.5 54 588.0 55 499.5 56 416.0 57 341.5 58 313.0 59 286.5 60 263.0 61 238.0 62 165.5 63 80.5 64 32.0 65 21.5 66 16.5 67 9.5 68 7.5 69 4.5 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02314883513874462 2 0.003561359252114557 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013948657070782014 12-13 0.0 14-15 0.0 16-17 0.0 18-19 8.903398130286393E-4 20-21 5.935598753524262E-4 22-23 0.0014838996883810654 24-25 0.010090517880991244 26-27 0.005638818815848048 28-29 2.967799376762131E-4 30-31 0.003264579314438344 32-33 0.0047484790028194095 34-35 0.0 36-37 0.011277637631696097 38-39 0.01068407775634367 40-41 8.903398130286393E-4 42-43 0.0 44-45 0.0 46-47 2.967799376762131E-4 48-49 0.004451699065143196 50-51 0.006529158628876688 52-53 0.0 54-55 0.0038581391897907704 56-57 0.003561359252114557 58-59 0.0 60-61 0.0011871197507048524 62-63 0.006825938566552901 64-65 0.0 66-67 0.0023742395014097048 68-69 8.903398130286393E-4 70-71 2.967799376762131E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0014838996883810654 80-81 0.0020774595637334916 82-83 0.01246475738240095 84-85 0.008309838254933966 86-87 0.003561359252114557 88-89 0.0 90-91 5.935598753524262E-4 92-93 0.0 94-95 0.0 96-97 0.003264579314438344 98-99 0.0017806796260572786 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 168475.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.636889746253154 #Duplication Level Percentage of deduplicated Percentage of total 1 73.96882643120674 21.18237127170203 2 9.534469178792024 5.460750853242321 3 3.618952866558886 3.1090666270960083 4 2.215727728723625 2.5380620270069745 5 1.5338059113708908 2.196171538803977 6 1.06951871657754 1.8376613740911114 7 0.8954110185300336 1.7949250630657367 8 0.6653401318244 1.5242617599050303 9 0.6632674211333582 1.7094524410149872 >10 4.933051444679352 29.056536578127318 >50 0.640467603531899 12.829796705742691 >100 0.25079799361605104 11.10194390859178 >500 0.0 0.0 >1k 0.010363553455208722 5.658999851610031 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2490 1.4779640896275412 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2377 1.410891823712717 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1883 1.1176732452886184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1650 0.9793737943315032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1134 0.6730968986496513 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 447 0.2653212642825345 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.25166938714942866 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 395 0.23445615076420837 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 321 0.1905327199881288 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 297 0.17628728297967056 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 281 0.16679032497403176 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 271 0.1608547262205075 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 270 0.16026116634515508 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 267 0.15848048671909779 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 263 0.1561062472176881 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 259 0.15373200771627837 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 229 0.1359252114557056 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 219 0.12998961270218132 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 207 0.12286689419795221 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 205 0.12167977444724737 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 204 0.12108621457189493 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 202 0.1198990948211901 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 190 0.11277637631696097 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 190 0.11277637631696097 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 187 0.1109956966909037 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 187 0.1109956966909037 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 187 0.1109956966909037 No Hit CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC 186 0.11040213681555128 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 184 0.10921501706484642 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.108621457189494 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 181 0.10743433743878913 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.10624721768808429 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.10506009793737944 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 176 0.104466538062027 No Hit TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA 175 0.10387297818667458 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 171 0.10149873868526488 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 169 0.10031161893456003 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 169 0.10031161893456003 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.935598753524262E-4 0.0 14-15 0.0 0.0 0.0 0.0011871197507048524 0.0 16-17 0.0 0.0 0.0 0.0011871197507048524 0.0 18-19 0.0 0.0 0.0 0.0014838996883810656 0.0 20-21 0.0 0.0 0.0 0.0017806796260572786 0.0 22-23 0.0 0.0 0.0 0.0035613592521145567 0.0 24-25 0.0 0.0 0.0 0.005045258940495623 0.0 26-27 0.0 0.0 0.0 0.011277637631696098 0.0 28-29 0.0 0.0 0.0 0.020477815699658702 0.0 30-31 0.0 0.0 0.0 0.037691052084879065 0.0 32-33 0.0 0.0 0.0 0.05401394865707078 0.0 34-35 0.0 0.0 0.0 0.0655883662264431 0.0 36-37 0.0 0.0 0.0 0.08339516248701588 0.0 38-39 0.0 0.0 0.0 0.11515061581837069 0.0 40-41 0.0 0.0 0.0 0.13800267101943908 0.0 42-43 0.0 0.0 0.0 0.1967650986793293 0.0 44-45 0.0 0.0 0.0 0.263243804718801 0.0 46-47 0.0 0.0 0.0 0.3234901320670723 0.0 48-49 0.0 0.0 0.0 0.3994657961121828 0.0 50-51 0.0 0.0 0.0 0.49532571598159963 0.0 52-53 0.0 0.0 0.0 0.6668645199584509 0.0 54-55 0.0 0.0 0.0 0.8265321264282535 0.0 56-57 0.0 0.0 0.0 1.0832467725181778 0.0 58-59 0.0 0.0 0.0 1.410891823712717 0.0 60-61 0.0 0.0 0.0 1.709155661077311 0.0 62-63 0.0 0.0 0.0 2.076272443982787 0.0 64-65 0.0 0.0 0.0 2.380471880100905 0.0 66-67 0.0 0.0 0.0 2.84404214275115 0.0 68-69 0.0 0.0 0.0 3.380620270069743 0.0 70-71 0.0 0.0 0.0 3.823415937082653 0.0 72-73 0.0 0.0 0.0 4.451105505267844 0.0 74-75 0.0 0.0 0.0 5.073453034574863 0.0 76-77 0.0 0.0 0.0 5.536132957412079 0.0 78-79 0.0 0.0 0.0 6.034426472770441 0.0 80-81 0.0 0.0 0.0 6.807834990354652 0.0 82-83 0.0 0.0 0.0 7.412375723401098 0.0 84-85 0.0 0.0 0.0 8.324083691942423 0.0 86-87 0.0 0.0 0.0 9.419795221843003 0.0 88-89 0.0 0.0 0.0 10.671019439085917 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTGTC 20 0.002072254 71.23909 2 TATAAGC 50 0.0016131164 37.99418 2 CGCAGAA 95 8.631723E-7 35.00503 9 ATAAGCC 55 0.0025753395 34.540165 3 GGGATGA 70 2.0441979E-4 33.93345 7 GTTCGTA 30 0.0039442447 31.67122 62-63 GTACGGC 30 0.003949982 31.66182 46-47 CCCTATA 75 3.0701436E-4 31.661818 2 CTGGTCG 140 1.6852937E-8 30.540104 9 ATTGAAC 125 2.1327833E-7 30.404371 8 GGATGAC 80 4.4737823E-4 29.691767 8 ATATAAG 80 4.4737823E-4 29.691767 1 GAAGGTA 160 1.877197E-9 29.691767 9 GCAGATC 65 0.005826011 29.234972 8 GTATAGT 115 3.834577E-6 28.9172 1 TCCACAC 150 3.2965545E-8 28.504099 8 CCCTGGT 150 3.2965545E-8 28.504099 7 GGTATCA 1420 0.0 28.437187 1 CCACACA 155 4.532376E-8 27.58461 9 GCGGTAT 70 0.008365735 27.146759 1 >>END_MODULE