FastQCFastQC Report
Thu 26 May 2016
SRR937116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179157
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25081.3998894824092836No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19931.1124321126163086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19871.109083094715808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.8768845202810942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.5844036236373683No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.2070809401809586No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3610.20149924368012412No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3270.18252147557728696No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3170.1769397790764525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.17582343977628562No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2880.16075285922403257No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2840.15852018062369877No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2720.1518221448226974No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA2670.14903129657228018No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA2500.1395424125208616No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA2450.13675156427044435No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC2370.13228620706977678No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT2370.13228620706977678No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2280.1272626802190258No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG2190.12223915336827475No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA2150.12000647476794098No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC2090.11665745686744028No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT2080.11609928721735684No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA2020.11275026931685617No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT1980.11051759071652237No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA1960.1094012514163555No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT1950.10884308176627204No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC1940.1082849121161886No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1880.10493589421568793No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.10326138526543759No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.10326138526543759No Hit
GGGTTATTCTATGGCCAATGCTCTGAAATTTGTGGATCTAACCATAGCTT1830.10214504596527069No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC1820.10158687631518723No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG1800.10047053701502034No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGG200.00206614171.294011
CATTCCG200.002068432771.274099
ATGGGTA652.5562913E-643.8977665
GTAGATA554.9203412E-543.2205666
CATGGGT704.255233E-640.7622154
ACCCTTG1650.037.4368977
AGATATA702.0423777E-433.9400448
CGCAGAA702.0423777E-433.9400449
TACAATC851.6207936E-533.540757
CCCTTGG1850.033.3896648
CCTTGGC1850.033.3896649
AGTGTAT753.0470433E-431.7039435
AACACAG600.003919477631.7039435
AACCCTT1950.031.6950826
TAGATAT753.0620734E-431.6773747
CATGGGC953.4467696E-530.0353134
GTACATG6750.028.1655311
GTATCAA16800.028.008361
ACAATCA856.3732936E-427.9506248
AGGGCGT700.00833642327.1672136
Produced by FastQC (version 0.11.5)