##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 179157 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.816429165480557 33.0 31.0 34.0 30.0 34.0 2 31.917251349375128 33.0 31.0 34.0 30.0 34.0 3 31.893590537908093 33.0 31.0 34.0 30.0 34.0 4 35.532789676094154 37.0 35.0 37.0 33.0 37.0 5 35.55165022857047 37.0 35.0 37.0 33.0 37.0 6 35.630742867987294 37.0 35.0 37.0 33.0 37.0 7 35.59279291347812 37.0 35.0 37.0 33.0 37.0 8 35.56247871978209 37.0 35.0 37.0 33.0 37.0 9 37.20652835222737 39.0 37.0 39.0 34.0 39.0 10-11 37.0919612406995 39.0 37.0 39.0 33.0 39.0 12-13 37.05199071205702 39.0 37.0 39.0 33.0 39.0 14-15 38.163300903676664 40.0 38.0 41.0 33.0 41.0 16-17 38.110414329331256 40.0 38.0 41.0 33.0 41.0 18-19 38.06768644261737 40.0 38.0 41.0 33.5 41.0 20-21 38.023454288696506 40.0 38.0 41.0 33.0 41.0 22-23 37.92292235301998 40.0 37.5 41.0 33.0 41.0 24-25 37.871863784278595 40.0 37.0 41.0 33.0 41.0 26-27 37.85152966392605 40.0 37.0 41.0 33.0 41.0 28-29 37.74394525472072 40.0 37.0 41.0 33.0 41.0 30-31 37.54979431448395 40.0 37.0 41.0 33.0 41.0 32-33 37.2712118421273 39.5 35.5 41.0 33.0 41.0 34-35 37.11902688703204 39.0 35.0 41.0 32.5 41.0 36-37 36.93466903330598 39.0 35.0 41.0 31.5 41.0 38-39 36.75488537986236 39.0 35.0 41.0 31.0 41.0 40-41 36.59109886859012 39.0 35.0 41.0 31.0 41.0 42-43 36.33946761778775 39.0 35.0 41.0 30.5 41.0 44-45 36.0367945433335 39.0 35.0 41.0 30.0 41.0 46-47 35.63824745893267 39.0 35.0 41.0 29.0 41.0 48-49 35.305310426050895 39.0 35.0 40.5 27.0 41.0 50-51 34.52370825588729 38.0 34.0 40.0 24.5 40.5 52-53 34.73278744341555 38.5 35.0 40.0 25.0 40.5 54-55 35.114754098360656 39.0 35.0 41.0 25.5 41.0 56-57 35.01210390886206 39.0 35.0 41.0 24.0 41.0 58-59 34.84791551544177 39.0 35.0 41.0 23.0 41.0 60-61 34.6033702283472 39.0 35.0 41.0 22.5 41.0 62-63 34.2969406721479 38.5 35.0 40.0 21.0 41.0 64-65 33.994889956853484 38.0 34.0 40.0 19.0 41.0 66-67 33.61816730577091 38.0 34.0 40.0 13.5 41.0 68-69 33.21549534765597 37.0 34.0 40.0 8.0 41.0 70-71 32.776679113849866 37.0 33.0 39.0 4.5 41.0 72-73 32.35182549384059 36.0 33.0 39.0 2.0 41.0 74-75 31.9207315371434 36.0 32.5 39.0 2.0 40.0 76-77 31.458039596554976 35.0 32.0 37.5 2.0 40.0 78-79 30.965996304916914 35.0 31.5 37.0 2.0 39.0 80-81 30.47151381190799 35.0 31.0 36.5 2.0 39.0 82-83 30.010644295227088 35.0 31.0 36.0 2.0 38.5 84-85 29.55570533107833 35.0 30.5 36.0 2.0 37.0 86-87 29.202392315120257 34.5 30.0 35.0 2.0 37.0 88-89 28.789634231428302 34.0 29.5 35.0 2.0 36.0 90-91 28.38722461304889 34.0 29.0 35.0 2.0 36.0 92-93 27.997482654878127 34.0 29.0 35.0 2.0 36.0 94-95 27.64668419319368 34.0 29.0 35.0 2.0 35.0 96-97 27.207128384601216 34.0 27.0 35.0 2.0 35.0 98-99 26.705529228553726 34.0 25.5 35.0 2.0 35.0 100-101 24.737093722265946 31.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18229079751848332 1101 2 0.15975487685389922 1101 3 0.30577319210872034 1101 4 0.008940510892955444 1101 5 0.06392116230076539 1101 6 -0.04634666621732464 1101 7 0.2479576904803551 1101 8 0.13431038393706984 1101 9 0.28563028075776487 1101 10-11 0.1863871832889501 1101 12-13 0.11575367403057868 1101 14-15 0.28442290587344843 1101 16-17 0.7044041233722211 1101 18-19 0.47252377047751537 1101 20-21 0.7149214458179429 1101 22-23 0.5898364175357997 1101 24-25 0.6185467874284782 1101 26-27 0.566308724198997 1101 28-29 0.5890456873136358 1101 30-31 0.6754934918927518 1101 32-33 0.6470438868577872 1101 34-35 0.8089946697502484 1101 36-37 0.9308881638871895 1101 38-39 0.8633950327149194 1101 40-41 0.8258713367540693 1101 42-43 0.7310275798560184 1101 44-45 0.7093591269026831 1101 46-47 0.9417141974176033 1101 48-49 0.8665191139561301 1101 50-51 1.307830167506495 1101 52-53 1.160925692689851 1101 54-55 1.3655021028208196 1101 56-57 0.9907896532045513 1101 58-59 0.9574961660752948 1101 60-61 1.1441652699496245 1101 62-63 1.1063533794619573 1101 64-65 1.318616320397986 1101 66-67 1.505797102129229 1101 68-69 1.4246190486033612 1101 70-71 1.2712286295083643 1101 72-73 1.4922385671379708 1101 74-75 1.3469858171215527 1101 76-77 1.4675822642039158 1101 78-79 1.4277591345364762 1101 80-81 1.5318664784268456 1101 82-83 1.6008765020935272 1101 84-85 1.4052460330620384 1101 86-87 1.4283624547194904 1101 88-89 1.4661752216828923 1101 90-91 1.6011197229190444 1101 92-93 1.4086596507554319 1101 94-95 1.5088292736593445 1101 96-97 1.497403879112813 1101 98-99 1.917344069337826 1101 100-101 2.0013702451354582 1102 1 0.320518500414952 1102 2 0.3051138291529121 1102 3 0.200209670456875 1102 4 0.10594844512485935 1102 5 0.07824101699917207 1102 6 0.2579454664659693 1102 7 0.3633290698895877 1102 8 0.33909960102912606 1102 9 0.4397168881566742 1102 10-11 0.3277503416868015 1102 12-13 0.1414252159481748 1102 14-15 -0.18772602826286544 1102 16-17 0.1220410605675184 1102 18-19 0.2170128250612109 1102 20-21 -0.15597521798092373 1102 22-23 0.14810522548915372 1102 24-25 -0.1549500102008139 1102 26-27 -0.27842887258906757 1102 28-29 -0.4313682310498095 1102 30-31 -0.1339415525580634 1102 32-33 -0.1692547461600853 1102 34-35 -0.126370018138104 1102 36-37 -0.27917301573234 1102 38-39 -0.197588620855484 1102 40-41 0.04155339391878243 1102 42-43 -0.1173329975266384 1102 44-45 0.28883049675260253 1102 46-47 0.3504370728597195 1102 48-49 0.530055104779187 1102 50-51 0.6023096627746298 1102 52-53 0.689541503797777 1102 54-55 0.427317416844339 1102 56-57 0.5016107984105531 1102 58-59 -0.026372488929013116 1102 60-61 0.27783317932730967 1102 62-63 0.29788860553977514 1102 64-65 0.41892699815345935 1102 66-67 0.5595539131415563 1102 68-69 0.9336526018009508 1102 70-71 0.8299132676882763 1102 72-73 0.6182829223621376 1102 74-75 0.9031420163376858 1102 76-77 0.8273136474368812 1102 78-79 0.7987179338864436 1102 80-81 0.6454311471965646 1102 82-83 1.1112483594685365 1102 84-85 0.9304061515975484 1102 86-87 1.0478538991320683 1102 88-89 1.0422625938270436 1102 90-91 0.7621129358050034 1102 92-93 0.7066521944893012 1102 94-95 0.43051458099134265 1102 96-97 0.4642856303794183 1102 98-99 0.5678220351154728 1102 100-101 0.40540905595660703 1103 1 0.18669469811615613 1103 2 0.2575004991669907 1103 3 0.24295469572620831 1103 4 0.19973426154829355 1103 5 0.16642719145039564 1103 6 0.0864414222801031 1103 7 0.03408540359768608 1103 8 0.0996139385139827 1103 9 -0.004052009270544943 1103 10-11 0.2373046625801365 1103 12-13 -0.07132297481077643 1103 14-15 0.23943496417270893 1103 16-17 0.2060084014470931 1103 18-19 0.32815971469477745 1103 20-21 0.3461314699345408 1103 22-23 0.3029904492019426 1103 24-25 0.37110524396616285 1103 26-27 0.43853269400082695 1103 28-29 0.17173940651255037 1103 30-31 0.5167957882123488 1103 32-33 0.3441603805140758 1103 34-35 0.6749955085884523 1103 36-37 0.605717774875977 1103 38-39 0.6288715976724575 1103 40-41 0.6767416313959913 1103 42-43 0.9556369958149133 1103 44-45 0.8268436608232008 1103 46-47 1.0532849219641065 1103 48-49 1.2804071290552201 1103 50-51 1.5308405481293335 1103 52-53 1.104875572106856 1103 54-55 1.1216675636575602 1103 56-57 1.3319241818823286 1103 58-59 1.4218717558834086 1103 60-61 1.3873864086724907 1103 62-63 1.4011053979164032 1103 64-65 1.5667499053827854 1103 66-67 1.6304117608269308 1103 68-69 1.6926278564045631 1103 70-71 1.771155231165075 1103 72-73 1.7161402133579564 1103 74-75 1.9421048272924644 1103 76-77 2.142170709207594 1103 78-79 1.843476865479115 1103 80-81 1.8380948521292666 1103 82-83 1.7202012373352176 1103 84-85 1.4425114205004341 1103 86-87 1.4935821254754948 1103 88-89 1.5535874058078782 1103 90-91 1.7774382717289434 1103 92-93 1.698121745753859 1103 94-95 1.9371348204770058 1103 96-97 1.8326612975882206 1103 98-99 1.7937779584014741 1103 100-101 2.1073836665468058 1104 1 0.1161117658222075 1104 2 0.26863679952892383 1104 3 0.19908562680046415 1104 4 0.6814587972043427 1104 5 0.5225766830252567 1104 6 0.5064920659317522 1104 7 0.369831602009441 1104 8 0.23403173538255828 1104 9 0.5910639275707226 1104 10-11 0.23856412512106573 1104 12-13 0.07258056361608567 1104 14-15 0.21801747222664147 1104 16-17 0.41527141699814507 1104 18-19 0.011046933138608495 1104 20-21 0.4483070257552484 1104 22-23 0.09838744505234587 1104 24-25 -0.00912649714135938 1104 26-27 -0.05082816190340367 1104 28-29 0.0843260774216148 1104 30-31 0.2860821369641542 1104 32-33 0.16696725850529504 1104 34-35 0.3877651330034695 1104 36-37 0.209205823239337 1104 38-39 0.5387868822447004 1104 40-41 0.4271426707840291 1104 42-43 0.5500105126320562 1104 44-45 0.6373807221656591 1104 46-47 0.8762840337115776 1104 48-49 0.9733634190762928 1104 50-51 1.1126194395371414 1104 52-53 1.1572161838079253 1104 54-55 1.3807058645763064 1104 56-57 1.2158680231105095 1104 58-59 1.23257453598125 1104 60-61 1.1085679171108964 1104 62-63 0.8310660231401172 1104 64-65 0.7482053137800833 1104 66-67 0.8429096064831114 1104 68-69 0.7217280698503146 1104 70-71 1.0427019837403435 1104 72-73 0.9480205246440612 1104 74-75 1.112032839065126 1104 76-77 1.0035105122011352 1104 78-79 0.8480308168771238 1104 80-81 0.6812917657831683 1104 82-83 1.1647915142844028 1104 84-85 0.8997427241104532 1104 86-87 0.9717271360152075 1104 88-89 0.8834340165278931 1104 90-91 1.0757452889239225 1104 92-93 1.3295234612744125 1104 94-95 1.1309992492776004 1104 96-97 1.1938685255774573 1104 98-99 1.0241535433886781 1104 100-101 1.251161258719577 1105 1 0.18109462526967945 1105 2 0.2940451479862709 1105 3 0.40250365462865645 1105 4 0.36037591759151866 1105 5 0.4468557715511636 1105 6 0.3558064438305166 1105 7 0.3229975628886521 1105 8 0.1260966678286195 1105 9 0.4699205518901408 1105 10-11 0.5404142965265848 1105 12-13 0.5371651572841643 1105 14-15 0.7893272120935464 1105 16-17 0.7541250165141733 1105 18-19 0.8530261628220188 1105 20-21 0.42221809653564435 1105 22-23 0.5068619358771045 1105 24-25 0.4796313169340536 1105 26-27 0.5721300958098468 1105 28-29 0.4614938531828514 1105 30-31 0.6551585636630861 1105 32-33 0.3053772201911187 1105 34-35 0.0068415597023943064 1105 36-37 0.1961991686718818 1105 38-39 0.6907076323959416 1105 40-41 0.48675650421818517 1105 42-43 0.4365299722005176 1105 44-45 0.4227961284975805 1105 46-47 0.28369984335061815 1105 48-49 0.9219417614840353 1105 50-51 1.0420406938222868 1105 52-53 0.9810213864697559 1105 54-55 0.8568099178128463 1105 56-57 1.0681819977021547 1105 58-59 1.2800639491693886 1105 60-61 1.2683279493754682 1105 62-63 1.5721428531461683 1105 64-65 1.7399879312432844 1105 66-67 1.4704302759729302 1105 68-69 1.6616605159079896 1105 70-71 1.5855029516774337 1105 72-73 1.5133310711252115 1105 74-75 1.833070346627128 1105 76-77 1.7589299516042338 1105 78-79 1.7737718937071314 1105 80-81 1.6393226187777223 1105 82-83 1.1652704414874648 1105 84-85 1.223068999569218 1105 86-87 1.2828281644483575 1105 88-89 1.159396912273003 1105 90-91 1.276239340143924 1105 92-93 1.4149993636740916 1105 94-95 1.781357184345154 1105 96-97 1.379541040197342 1105 98-99 1.3073759672644591 1105 100-101 1.7020082036681572 1106 1 0.1898665550942411 1106 2 0.20131331841234257 1106 3 0.47330565964118776 1106 4 0.3165162684687104 1106 5 0.578748002127611 1106 6 0.39120744633678584 1106 7 0.32111786364053074 1106 8 0.174028998655686 1106 9 0.05576015088763597 1106 10-11 0.3594932438950096 1106 12-13 0.2777666610435645 1106 14-15 0.20098134743189888 1106 16-17 0.5143067207748189 1106 18-19 0.6174371903909375 1106 20-21 0.7687093246058225 1106 22-23 0.6625636902630703 1106 24-25 0.41243206881375016 1106 26-27 0.7024559103461883 1106 28-29 0.2782231764911245 1106 30-31 0.6108289897282475 1106 32-33 0.4809724582863595 1106 34-35 0.7660834143389934 1106 36-37 0.44760894310797994 1106 38-39 0.4507326950525794 1106 40-41 0.97062241900516 1106 42-43 1.1682029045313485 1106 44-45 0.9202898628334211 1106 46-47 0.8400908207942308 1106 48-49 1.2823740923111089 1106 50-51 1.3399730246493533 1106 52-53 1.2241291558933156 1106 54-55 1.1237272110459244 1106 56-57 0.9732571856720824 1106 58-59 1.1863922699713925 1106 60-61 1.1232778240621784 1106 62-63 1.3129009985095834 1106 64-65 1.699731039012704 1106 66-67 1.1883187471508734 1106 68-69 1.3678008667851813 1106 70-71 1.2637610970408346 1106 72-73 1.6469463593457583 1106 74-75 1.4712282413639777 1106 76-77 1.2055151646368252 1106 78-79 0.8456703899477311 1106 80-81 1.1449617165220864 1106 82-83 1.3289609176779393 1106 84-85 1.242962483278486 1106 86-87 1.5752780391350747 1106 88-89 1.8658505463582138 1106 90-91 1.8063145281138482 1106 92-93 2.062555754651534 1106 94-95 2.505041394871469 1106 96-97 2.462717731926663 1106 98-99 2.8844436364373927 1106 100-101 2.6183615871268024 1107 1 0.36410897933666675 1107 2 0.11872923582825834 1107 3 0.026752213087743115 1107 4 0.22385892581137057 1107 5 0.2531985515781585 1107 6 0.4007395808689225 1107 7 0.42068496320762705 1107 8 0.3713733210000072 1107 9 0.3814344765619566 1107 10-11 0.7427974396992099 1107 12-13 0.7974553057322069 1107 14-15 0.5598308312813813 1107 16-17 0.5204880394561471 1107 18-19 0.7904724884262322 1107 20-21 0.8744785553750489 1107 22-23 0.7424005200998991 1107 24-25 0.6093393282210187 1107 26-27 0.9900849479752267 1107 28-29 0.06934871761666983 1107 30-31 0.25591307231233884 1107 32-33 0.08364062345452083 1107 34-35 0.4881321988877687 1107 36-37 0.43776462076365874 1107 38-39 0.5147936341135164 1107 40-41 0.6959179568006917 1107 42-43 0.896994945823387 1107 44-45 0.5721796397231955 1107 46-47 1.122017227720633 1107 48-49 1.2913442887813034 1107 50-51 1.078109887786212 1107 52-53 1.085517767281921 1107 54-55 1.001516088834805 1107 56-57 1.3912558536707493 1107 58-59 1.0956080541871742 1107 60-61 0.8322771580615154 1107 62-63 0.7050713281799048 1107 64-65 0.8201314719131361 1107 66-67 0.7404124867446171 1107 68-69 0.6588847828691016 1107 70-71 0.738827697107439 1107 72-73 0.3767374016368059 1107 74-75 0.4741419942777263 1107 76-77 0.8320344786561336 1107 78-79 0.8540994608767996 1107 80-81 1.105293051853419 1107 82-83 0.31649421771124153 1107 84-85 0.43508286289886655 1107 86-87 0.32929485565189154 1107 88-89 0.38354118904885937 1107 90-91 0.4026348744341952 1107 92-93 0.6527155948113759 1107 94-95 0.3450763599064359 1107 96-97 0.30148313319206466 1107 98-99 0.8948498968436525 1107 100-101 1.1140742078394261 1108 1 -0.11782575259806904 1108 2 0.031899398998419315 1108 3 -0.1906137542782247 1108 4 0.08574703769947689 1108 5 0.12499342337302721 1108 6 0.21950414963348663 1108 7 0.01800431052697604 1108 8 0.2111169107435984 1108 9 0.16290300803049007 1108 10-11 -0.16134008943832612 1108 12-13 -0.1256216173447129 1108 14-15 -0.09343257198202082 1108 16-17 -0.3416914477233419 1108 18-19 -0.15270932975558082 1108 20-21 -0.19923939334289287 1108 22-23 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-1.6954848573684949 2316 90-91 -1.9297103166066485 2316 92-93 -1.7231585310627509 2316 94-95 -2.5257557065778045 2316 96-97 -2.81096239229694 2316 98-99 -3.5700077093183005 2316 100-101 -4.465230545378372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 471.0 3 122.0 4 106.0 5 111.0 6 135.0 7 153.0 8 185.0 9 248.0 10 325.0 11 449.0 12 626.0 13 841.0 14 998.0 15 1395.0 16 1812.0 17 2472.0 18 2320.0 19 1229.0 20 1170.0 21 1232.0 22 1292.0 23 1456.0 24 1566.0 25 1697.0 26 1835.0 27 2119.0 28 2381.0 29 2801.0 30 3406.0 31 4278.0 32 5403.0 33 7082.0 34 9559.0 35 14460.0 36 19501.0 37 33192.0 38 41680.0 39 9048.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.6311474494298 24.742820761987737 17.8137810095053 20.81225077907717 2 13.566694398999402 24.741335507250422 41.124443724768696 20.56752636898148 3 14.506024634557615 31.246580084646396 33.6246077566472 20.62278752414879 4 9.796201039938786 22.079989276678454 42.99660986657284 25.127199816809924 5 9.392567756356524 40.282201732327465 38.24140821458508 12.083822296730931 6 22.396601833221553 44.3242790073776 20.138609434384083 13.140509725016766 7 21.0543953619562 36.93902513949319 27.05357990627845 14.952999592272162 8 20.91197604121269 36.91485914155129 24.061036798641144 18.11212801859488 9 23.26175126534899 18.94615702617848 25.063965765745632 32.7281259427269 10-11 19.130060725917733 30.581393400036873 31.774682823000987 18.513863051044407 12-13 21.274105606578626 29.78480927800496 31.05321668789524 17.887868427521173 14-15 19.406770193274493 31.75399396715451 29.34141436710982 19.497821472461176 16-17 16.119744760468592 34.11217693628786 31.004900934109664 18.76317736913389 18-19 15.889413946421788 32.48234912974507 34.52420850369774 17.104028420135396 20-21 16.83232190084145 31.740734431247258 34.289656019861866 17.13728764804942 22-23 17.271618139331014 30.99421245564418 34.37739926238822 17.356770142636584 24-25 17.01355647414445 31.782214419488724 34.069258873671075 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16.570242052295352 31.685044556265368 32.77376357410183 18.970949817337452 52-53 16.527211833658946 32.36645269327379 32.991906223834775 18.114429249232487 54-55 17.40860968373103 31.724867488577086 32.53933474939362 18.32718807829827 56-57 17.387457541021583 32.25458919804514 31.744383692403016 18.613569568530256 58-59 16.869433675790486 31.296732004253503 32.86639705492432 18.96743726503169 60-61 17.36025252088293 32.380612156614305 32.037889296050594 18.221246026452175 62-63 16.824456712868635 32.53861483412507 32.757995098832765 17.87893335417353 64-65 17.49515077936395 31.381923221836143 32.73077406120484 18.392151937595067 66-67 16.914646537959065 32.0312761704836 31.876905211206267 19.177172080351063 68-69 16.947283185346514 31.417881275478866 32.765119785558724 18.869715753615903 70-71 17.660020107238605 30.997542448614833 32.83595844504021 18.506478999106346 72-73 17.145783677274224 31.194703849867988 32.18242915115351 19.47708332170428 74-75 16.85275333839489 31.791233084721537 31.94949979902639 19.406513777857175 76-77 16.57473061247278 31.30338897883982 32.68801295293395 19.433867455753447 78-79 16.66038531223915 30.72308160456501 32.43216835146551 20.18436473173033 80-81 17.25720446806906 31.864181426620146 31.279835310482756 19.598778794828036 82-83 17.639685313709176 31.054891428762865 31.698111100564493 19.607312156963467 84-85 16.81934396212933 30.659108163629867 31.684306895319757 20.83724097892104 86-87 17.295848571986934 31.193221474636367 31.64521622602529 19.865713727351405 88-89 17.790175891254027 30.05956120730818 31.9145312033151 20.23573169812269 90-91 17.505995873387256 29.272984646655292 32.76441173432208 20.456607745635367 92-93 18.360663061171497 30.370759192197834 31.50043137506736 19.768146371563308 94-95 17.616372055634173 29.286374204857246 32.43233436328568 20.664919376222894 96-97 17.794048273686737 29.585567580090945 31.8736409592211 20.746743187001222 98-99 18.09485517079627 30.397938270984458 31.45672435263831 20.050482205580963 100-101 17.94315245478036 30.264124589705986 31.27201620224876 20.520706753264893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1921.0 1 1206.5 2 449.5 3 374.5 4 301.0 5 281.5 6 418.5 7 710.0 8 950.5 9 965.0 10 746.5 11 553.5 12 551.0 13 584.5 14 566.5 15 529.5 16 560.5 17 559.0 18 535.0 19 569.0 20 650.0 21 705.5 22 716.5 23 829.0 24 947.5 25 1136.0 26 1550.0 27 1981.0 28 2067.5 29 2389.5 30 3359.5 31 4249.5 32 5562.0 33 7256.5 34 8376.5 35 9570.5 36 11608.0 37 12954.5 38 12407.5 39 11101.5 40 9570.0 41 8053.5 42 6466.5 43 6016.0 44 6180.0 45 5658.5 46 4613.5 47 3881.5 48 4059.5 49 3433.5 50 2266.0 51 1446.5 52 1021.5 53 828.5 54 656.5 55 568.0 56 503.5 57 408.5 58 362.0 59 330.0 60 280.5 61 243.0 62 181.5 63 112.0 64 67.0 65 48.0 66 30.5 67 18.0 68 16.0 69 11.0 70 6.5 71 6.5 72 5.5 73 2.0 74 2.5 75 2.5 76 1.5 77 1.0 78 0.5 79 0.5 80 1.5 81 2.0 82 0.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055258795358261184 2 0.035722857605340566 3 0.03293200935492334 4 0.06028232220901221 5 0.11554111756727341 6 0.13228620706977678 7 0.06474767940967978 8 0.10214504596527069 9 0.08651629576293418 10-11 0.08707446541301764 12-13 0.08539995646276728 14-15 0.07646924206143216 16-17 0.061119576684137374 18-19 0.07200388486076459 20-21 0.06725944283505528 22-23 0.03656011208046574 24-25 0.009767968876460312 26-27 0.005302611675792741 28-29 0.006698035801001356 30-31 0.005302611675792741 32-33 0.009209799226376865 34-35 0.007256205451084802 36-37 0.006418950975959633 38-39 0.002511763425375509 40-41 0.006977120626043079 42-43 0.003628102725542401 44-45 0.001395424125208616 46-47 0.0011163393001668927 48-49 0.0 50-51 8.372544751251695E-4 52-53 0.003907187550584125 54-55 0.012000647476794097 56-57 0.007535290276126526 58-59 0.005860781325876187 60-61 0.0019535937752920623 62-63 0.008930714401335142 64-65 0.002511763425375509 66-67 0.024001294953588194 68-69 0.04856075955725983 70-71 0.06530584905976322 72-73 0.004465357200667571 74-75 0.016186919852419945 76-77 0.026233973553921982 78-79 0.030978415579631272 80-81 0.08567904128780902 82-83 0.03237383970483989 84-85 0.011721562651752374 86-87 0.034606518305173674 88-89 0.05442154088313602 90-91 0.0421418085813002 92-93 0.04381631753155054 94-95 0.012558817126877543 96-97 0.018140513627712004 98-99 0.046886250607009496 100-101 0.09460975568914416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 179157.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.5612228380694 #Duplication Level Percentage of deduplicated Percentage of total 1 75.65378297237591 22.364183369893446 2 8.789486603349635 5.196559442276886 3 3.432714639074036 3.0442572715551166 4 2.0581182379486793 2.4336196743638263 5 1.4048073110401995 2.0763910983104203 6 1.008289118407885 1.7883755588673622 7 0.8024772946130172 1.660554708998253 8 0.6476463812994467 1.5316175198289768 9 0.5494609240762071 1.4618463135685462 >10 4.741224674760673 29.118036135903147 >50 0.6665281999962237 13.565197005977996 >100 0.2360227337097109 10.676669066796162 >500 0.0 0.0 >1k 0.009440909348388437 5.082692833659863 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2508 1.3998894824092836 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1993 1.1124321126163086 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1987 1.109083094715808 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.8768845202810942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1047 0.5844036236373683 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 371 0.2070809401809586 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 361 0.20149924368012412 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 327 0.18252147557728696 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 317 0.1769397790764525 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.17582343977628562 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 288 0.16075285922403257 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 284 0.15852018062369877 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 272 0.1518221448226974 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 267 0.14903129657228018 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 250 0.1395424125208616 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 245 0.13675156427044435 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 237 0.13228620706977678 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 237 0.13228620706977678 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 228 0.1272626802190258 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 219 0.12223915336827475 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 215 0.12000647476794098 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 209 0.11665745686744028 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 208 0.11609928721735684 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 202 0.11275026931685617 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 198 0.11051759071652237 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 196 0.1094012514163555 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 195 0.10884308176627204 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 194 0.1082849121161886 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 188 0.10493589421568793 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 185 0.10326138526543759 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 185 0.10326138526543759 No Hit GGGTTATTCTATGGCCAATGCTCTGAAATTTGTGGATCTAACCATAGCTT 183 0.10214504596527069 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 182 0.10158687631518723 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 180 0.10047053701502034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.581696500834463E-4 7 0.0 0.0 0.0 0.0 5.581696500834463E-4 8 0.0 0.0 0.0 0.0 5.581696500834463E-4 9 0.0 0.0 0.0 0.0 5.581696500834463E-4 10-11 0.0 0.0 0.0 0.0 5.581696500834463E-4 12-13 0.0 0.0 0.0 0.0 5.581696500834463E-4 14-15 0.0 0.0 0.0 2.7908482504172317E-4 5.581696500834463E-4 16-17 0.0 0.0 0.0 5.581696500834463E-4 5.581696500834463E-4 18-19 0.0 0.0 0.0 0.0011163393001668927 5.581696500834463E-4 20-21 0.0 0.0 0.0 0.0011163393001668927 5.581696500834463E-4 22-23 0.0 0.0 0.0 0.0019535937752920623 5.581696500834463E-4 24-25 0.0 0.0 0.0 0.00530261167579274 5.581696500834463E-4 26-27 0.0 0.0 0.0 0.007256205451084803 5.581696500834463E-4 28-29 0.0 0.0 0.0 0.016466004677461666 5.581696500834463E-4 30-31 0.0 0.0 0.0 0.036839196905507465 5.581696500834463E-4 32-33 0.0 0.0 0.0 0.06279408563438771 5.581696500834463E-4 34-35 0.0 0.0 0.0 0.08121368408714144 5.581696500834463E-4 36-37 0.0 0.0 0.0 0.10214504596527069 5.581696500834463E-4 38-39 0.0 0.0 0.0 0.12307640784339993 5.581696500834463E-4 40-41 0.0 0.0 0.0 0.14372868489648744 5.581696500834463E-4 42-43 0.0 0.0 0.0 0.20736002500600031 5.581696500834463E-4 44-45 0.0 0.0 0.0 0.2807593339919735 5.581696500834463E-4 46-47 0.0 0.0 0.0 0.33518087487510956 5.581696500834463E-4 48-49 0.0 0.0 0.0 0.40746384456091583 5.581696500834463E-4 50-51 0.0 0.0 0.0 0.50207360025006 5.581696500834463E-4 52-53 0.0 0.0 0.0 0.6700826649251774 5.581696500834463E-4 54-55 0.0 0.0 0.0 0.8068342291956216 5.581696500834463E-4 56-57 0.0 0.0 0.0 1.0368001250300016 5.581696500834463E-4 58-59 0.0 0.0 0.0 1.3477006201264814 5.581696500834463E-4 60-61 0.0 0.0 0.0 1.6206455790172867 5.581696500834463E-4 62-63 0.0 0.0 0.0 1.9614081503932304 5.581696500834463E-4 64-65 0.0 0.0 0.0 2.245516502285705 5.581696500834463E-4 66-67 0.0 0.0 0.0 2.6945639857778376 5.581696500834463E-4 68-69 0.0 0.0 0.0 3.1963585012028553 5.581696500834463E-4 70-71 0.0 0.0 0.0 3.6228001138666084 5.581696500834463E-4 72-73 0.0 0.0 0.0 4.183481527375431 5.581696500834463E-4 74-75 0.0 0.0 0.0 4.760908030386755 5.581696500834463E-4 76-77 0.0 0.0 0.0 5.162511093621795 5.581696500834463E-4 78-79 0.0 0.0 0.0 5.602627862712593 5.581696500834463E-4 80-81 0.0 0.0 0.0 6.30173534944211 5.581696500834463E-4 82-83 0.0 0.0 0.0 6.862416762950931 5.581696500834463E-4 84-85 0.0 0.0 0.0 7.699392153251059 5.581696500834463E-4 86-87 0.0 0.0 0.0 8.680654398097758 5.581696500834463E-4 88-89 0.0 0.0 0.0 9.944908655536764 5.581696500834463E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCAGG 20 0.002066141 71.29401 1 CATTCCG 20 0.0020684327 71.27409 9 ATGGGTA 65 2.5562913E-6 43.897766 5 GTAGATA 55 4.9203412E-5 43.220566 6 CATGGGT 70 4.255233E-6 40.762215 4 ACCCTTG 165 0.0 37.436897 7 AGATATA 70 2.0423777E-4 33.940044 8 CGCAGAA 70 2.0423777E-4 33.940044 9 TACAATC 85 1.6207936E-5 33.54075 7 CCCTTGG 185 0.0 33.389664 8 CCTTGGC 185 0.0 33.389664 9 AGTGTAT 75 3.0470433E-4 31.703943 5 AACACAG 60 0.0039194776 31.703943 5 AACCCTT 195 0.0 31.695082 6 TAGATAT 75 3.0620734E-4 31.677374 7 CATGGGC 95 3.4467696E-5 30.035313 4 GTACATG 675 0.0 28.165531 1 GTATCAA 1680 0.0 28.00836 1 ACAATCA 85 6.3732936E-4 27.950624 8 AGGGCGT 70 0.008336423 27.167213 6 >>END_MODULE