##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937116_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 179157 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.930736728121143 31.0 31.0 34.0 30.0 34.0 2 32.095625624452296 33.0 31.0 34.0 30.0 34.0 3 32.165581026697254 33.0 31.0 34.0 30.0 34.0 4 35.91510797791881 37.0 35.0 37.0 35.0 37.0 5 35.72829975942888 37.0 35.0 37.0 33.0 37.0 6 35.82653761784357 37.0 35.0 37.0 35.0 37.0 7 35.77492367030035 37.0 35.0 37.0 33.0 37.0 8 35.74429131990377 37.0 35.0 37.0 33.0 37.0 9 37.38837444252806 39.0 37.0 39.0 34.0 39.0 10-11 37.31093398527548 39.0 37.0 39.0 34.0 39.0 12-13 37.24698727931367 39.0 37.0 39.0 33.5 39.0 14-15 38.34547910491915 40.0 38.0 41.0 33.0 41.0 16-17 38.2956345551667 40.0 38.0 41.0 33.5 41.0 18-19 38.383451386214325 40.0 38.0 41.0 34.0 41.0 20-21 38.40524791105008 40.0 38.0 41.0 34.0 41.0 22-23 38.28263478401625 40.0 37.5 41.0 34.0 41.0 24-25 38.18237914231651 40.0 37.0 41.0 33.5 41.0 26-27 37.93430901388167 40.0 37.0 41.0 33.0 41.0 28-29 37.76540408691818 40.0 37.0 41.0 33.0 41.0 30-31 37.64229697974402 39.0 36.5 41.0 33.0 41.0 32-33 37.39890989467339 39.0 35.5 41.0 33.0 41.0 34-35 37.21329336838639 39.0 35.0 41.0 32.5 41.0 36-37 37.01517105108927 39.0 35.0 41.0 32.0 41.0 38-39 36.810543266520426 39.0 35.0 41.0 31.0 41.0 40-41 36.403280363033545 39.0 35.0 41.0 30.5 41.0 42-43 36.16963612920511 39.0 35.0 40.0 30.0 41.0 44-45 35.8883214164113 39.0 35.0 40.0 30.0 41.0 46-47 35.411890129886075 38.5 35.0 40.0 27.5 41.0 48-49 34.95399007574362 38.0 35.0 40.0 24.5 41.0 50-51 34.64323749560441 38.0 35.0 40.0 24.0 41.0 52-53 34.414362821435944 38.0 34.0 40.0 23.0 41.0 54-55 34.03239337564259 38.0 34.0 40.0 21.0 41.0 56-57 33.805441595918666 38.0 33.5 40.0 20.5 41.0 58-59 33.48715372550333 38.0 33.0 40.0 18.5 41.0 60-61 33.09181053489398 37.5 33.0 40.0 13.5 41.0 62-63 32.70654509731688 37.0 32.5 39.5 8.5 40.5 64-65 32.392211858872386 37.0 32.0 39.0 7.5 40.0 66-67 32.07887216240504 36.0 31.5 39.0 3.5 40.0 68-69 31.58120252069414 36.0 31.0 39.0 2.0 40.0 70-71 31.12051999084602 35.0 31.0 38.0 2.0 40.0 72-73 30.67210882075498 35.0 30.0 38.0 2.0 40.0 74-75 30.140650379276277 35.0 29.5 37.0 2.0 39.0 76-77 28.68420435707229 33.0 28.0 35.5 2.0 38.0 78-79 29.311126553804762 34.0 29.0 36.0 2.0 39.0 80-81 29.092078456326014 34.0 29.0 36.0 2.0 37.5 82-83 28.708197279481126 34.0 29.0 35.5 2.0 37.0 84-85 28.201580178279386 34.0 28.0 35.0 2.0 36.5 86-87 27.804339210859748 34.0 27.0 35.0 2.0 36.0 88-89 27.35806303968028 33.0 26.0 35.0 2.0 36.0 90-91 26.873404332512823 33.0 26.0 35.0 2.0 35.0 92-93 26.344586033479015 33.0 25.0 35.0 2.0 35.0 94-95 25.76843494811813 32.0 23.5 34.0 2.0 35.0 96-97 24.999014830567603 32.0 19.0 34.0 2.0 35.0 98-99 24.20797401162109 31.5 4.5 34.0 2.0 35.0 100-101 21.31864788983964 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1505919485444096 1101 2 0.26285043069055547 1101 3 0.2583111364520576 1101 4 0.3214890590238255 1101 5 0.19024386595503984 1101 6 0.09670331247029651 1101 7 -0.17040006195003343 1101 8 0.13874444470156533 1101 9 0.19740454847933364 1101 10-11 0.2505398950498261 1101 12-13 0.35719176046285384 1101 14-15 0.5320363235653289 1101 16-17 0.48751420614853913 1101 18-19 0.11145904100939674 1101 20-21 -0.03430150704607371 1101 22-23 0.014639467079234691 1101 24-25 -0.08013991053696401 1101 26-27 0.26349924588334517 1101 28-29 0.42154536281312005 1101 30-31 0.5054192531179851 1101 32-33 0.5180901829726849 1101 34-35 0.3953154597933377 1101 36-37 0.673548624861219 1101 38-39 0.8293058207323938 1101 40-41 1.0858212811543666 1101 42-43 0.7567069435190774 1101 44-45 0.9017228346131603 1101 46-47 0.901178714206452 1101 48-49 1.4030777073031047 1101 50-51 1.347633942382096 1101 52-53 1.6712085079737093 1101 54-55 1.5415137524860825 1101 56-57 1.5174821594857661 1101 58-59 1.4388902520343265 1101 60-61 1.379558202104029 1101 62-63 1.3267811713703068 1101 64-65 1.285542962631169 1101 66-67 1.6970194528414488 1101 68-69 1.3149079645524644 1101 70-71 1.355502572443065 1101 72-73 1.4596365975639038 1101 74-75 1.5583714450395334 1101 76-77 1.867820880520295 1101 78-79 2.0728880983224656 1101 80-81 1.6763534003584581 1101 82-83 2.4368049642092977 1101 84-85 1.9355685437431838 1101 86-87 1.8705994888442206 1101 88-89 1.7257837216387983 1101 90-91 2.3410608842922613 1101 92-93 2.5094990801280055 1101 94-95 2.30843950864989 1101 96-97 2.3215823951960814 1101 98-99 2.7647800091988763 1101 100-101 3.0192399815109034 1102 1 -0.08878287221842385 1102 2 0.07944540249057752 1102 3 5.633111963447845E-4 1102 4 0.22775041071104596 1102 5 -0.1332146944120396 1102 6 0.015525604794163428 1102 7 -0.06879409693712546 1102 8 -0.013325147115658353 1102 9 -0.16985479575161833 1102 10-11 0.12537499685653586 1102 12-13 0.29685814035005365 1102 14-15 0.1421309607893093 1102 16-17 0.12827034800877612 1102 18-19 -0.10142691635605416 1102 20-21 0.0748371986226033 1102 22-23 0.24233280423629822 1102 24-25 0.16863649707225647 1102 26-27 0.07733157548854308 1102 28-29 -0.10844698973443911 1102 30-31 -0.2181587986935014 1102 32-33 -0.36437803230310095 1102 34-35 0.0575370447278587 1102 36-37 0.21912744141796026 1102 38-39 0.1966368598300008 1102 40-41 0.38728863609175335 1102 42-43 0.6635992100327925 1102 44-45 0.3734177012606992 1102 46-47 0.3888567564586296 1102 48-49 0.34036859727538626 1102 50-51 0.527444667934148 1102 52-53 0.6141059365178236 1102 54-55 1.2000645602482507 1102 56-57 1.2944967852385645 1102 58-59 1.1164258604847603 1102 60-61 1.104015710946392 1102 62-63 1.2297588981650662 1102 64-65 1.3692538889288386 1102 66-67 1.41390598376584 1102 68-69 1.4472949256460517 1102 70-71 1.1470234595475475 1102 72-73 0.6538914489165464 1102 74-75 1.1842580715570747 1102 76-77 1.4077979381629682 1102 78-79 1.0738010129577766 1102 80-81 1.6028948399913787 1102 82-83 1.7474922789975587 1102 84-85 1.5230028234487598 1102 86-87 1.7442683541534656 1102 88-89 1.4460685892422767 1102 90-91 1.6419260023498055 1102 92-93 1.7642787379045082 1102 94-95 1.4613216422891213 1102 96-97 1.662849960438411 1102 98-99 1.8302613207369696 1102 100-101 1.642731999482418 1103 1 0.27298892872686054 1103 2 0.40532710050286624 1103 3 0.6096690058030063 1103 4 0.43638892271261653 1103 5 0.30150526891194573 1103 6 0.4168878568762935 1103 7 0.3200371824664501 1103 8 0.20809539363729357 1103 9 0.24843736802426264 1103 10-11 0.2070828330806549 1103 12-13 0.46854228997946734 1103 14-15 0.47598096024353254 1103 16-17 0.5195630684742198 1103 18-19 0.23565952334136853 1103 20-21 0.333937981262622 1103 22-23 0.4279560236483917 1103 24-25 0.615587257335541 1103 26-27 0.30019841753061627 1103 28-29 0.579168305038138 1103 30-31 0.8750375817328546 1103 32-33 0.645069316872231 1103 34-35 0.7846936709708601 1103 36-37 1.0395022790501756 1103 38-39 0.7102484651698475 1103 40-41 1.0797449468773408 1103 42-43 1.0667625165504262 1103 44-45 0.9501971976203905 1103 46-47 0.9483843344224496 1103 48-49 0.9312836420834358 1103 50-51 0.9964228697177404 1103 52-53 1.0134597400173249 1103 54-55 1.0829523600146587 1103 56-57 0.9192279915084214 1103 58-59 1.1712379504719905 1103 60-61 0.941249509643086 1103 62-63 0.9606649922783461 1103 64-65 0.9718594143455377 1103 66-67 1.695388961072549 1103 68-69 1.5655423359480842 1103 70-71 1.8569390828761208 1103 72-73 1.4950984828527787 1103 74-75 1.5059755134048487 1103 76-77 1.5819762752985262 1103 78-79 1.1831795946001193 1103 80-81 1.33692276179287 1103 82-83 1.9865795264406039 1103 84-85 2.0227081054502207 1103 86-87 1.593536471123766 1103 88-89 1.8811051015276519 1103 90-91 1.9420307909715078 1103 92-93 1.7366407389305643 1103 94-95 2.006206101918213 1103 96-97 2.5386317817956403 1103 98-99 2.2014477244133026 1103 100-101 2.238171511342088 1104 1 0.03422134596691251 1104 2 0.13446310919978544 1104 3 0.15023032837125072 1104 4 0.118911559894606 1104 5 0.08237204429011058 1104 6 0.011645841205925933 1104 7 -0.12724436707019748 1104 8 0.08621919217631557 1104 9 0.10513704586700356 1104 10-11 0.05536399814979376 1104 12-13 0.06786748320753588 1104 14-15 0.11900950364196206 1104 16-17 0.06760825003568272 1104 18-19 0.30407485431486947 1104 20-21 0.08732858699782042 1104 22-23 0.43118422500330666 1104 24-25 0.18908683972427553 1104 26-27 0.208814467060634 1104 28-29 0.18060144083471386 1104 30-31 0.2545136453158321 1104 32-33 0.1239766336867234 1104 34-35 0.5050333281319013 1104 36-37 0.2791912580900586 1104 38-39 0.4949798019236198 1104 40-41 0.4552570178314781 1104 42-43 0.49320990415996846 1104 44-45 0.5070519882181728 1104 46-47 0.4210672547846457 1104 48-49 0.8022417616395714 1104 50-51 0.9702393063667714 1104 52-53 1.1096097618922016 1104 54-55 1.005846737508726 1104 56-57 1.1339398397365557 1104 58-59 0.6305307953990109 1104 60-61 1.0002652728110988 1104 62-63 1.269849788576856 1104 64-65 1.1041427536447515 1104 66-67 0.9663855273799378 1104 68-69 1.1804252059317726 1104 70-71 1.1502082499073616 1104 72-73 1.0436143056795402 1104 74-75 1.0882913335801092 1104 76-77 1.013832723083862 1104 78-79 1.2336508987404393 1104 80-81 1.2456846438276301 1104 82-83 1.3095322369365725 1104 84-85 0.8575991950841804 1104 86-87 1.117360199398032 1104 88-89 1.1776820149164031 1104 90-91 1.4023113196806278 1104 92-93 1.2183984183376886 1104 94-95 1.7197247751075686 1104 96-97 1.7440832659728152 1104 98-99 1.9453791035910761 1104 100-101 2.0609850180835245 1105 1 0.11079928506753589 1105 2 -0.09113681013879216 1105 3 0.10783266755253607 1105 4 0.18959113238906156 1105 5 0.3522853332596654 1105 6 0.14231734755801284 1105 7 0.18294922034661454 1105 8 0.14616071264415353 1105 9 0.24836148627837673 1105 10-11 0.16110608324759568 1105 12-13 0.3766494957100619 1105 14-15 0.38115913058287276 1105 16-17 0.09520958094918086 1105 18-19 0.2902867922055705 1105 20-21 0.31721364446908495 1105 22-23 0.2844560540010903 1105 24-25 0.18265913252523802 1105 26-27 0.19870658240646577 1105 28-29 -0.07685974085514147 1105 30-31 0.18113695646727024 1105 32-33 0.47044265506200134 1105 34-35 0.13714161609318154 1105 36-37 0.020797428688972275 1105 38-39 -0.03345334992150839 1105 40-41 0.0023683305961483825 1105 42-43 -0.04054186030867868 1105 44-45 -0.09700124832145463 1105 46-47 -0.02912431609657773 1105 48-49 0.10343655897774795 1105 50-51 0.19631815290841104 1105 52-53 0.30705380302952534 1105 54-55 0.3699826520076144 1105 56-57 0.4575938022289918 1105 58-59 0.6666015757982819 1105 60-61 0.45567462953624016 1105 62-63 0.6403377901900811 1105 64-65 0.956834828659872 1105 66-67 0.557785003080685 1105 68-69 -0.11623238632472876 1105 70-71 0.12575775585614224 1105 72-73 0.21015493887171743 1105 74-75 0.341744650781159 1105 76-77 0.20558005117420208 1105 78-79 0.42073498827462075 1105 80-81 0.24409662204904947 1105 82-83 0.20646206149797308 1105 84-85 0.04390187707651805 1105 86-87 0.7502246882062629 1105 88-89 0.38036904859254506 1105 90-91 0.4964835318201679 1105 92-93 0.6773619190434772 1105 94-95 1.0472353619194266 1105 96-97 1.6758487428834847 1105 98-99 1.9495886216390694 1105 100-101 2.2510182909207934 1106 1 -0.16802277007030852 1106 2 -0.4031668853267618 1106 3 -0.06603951289859111 1106 4 -0.04474470720993651 1106 5 0.04056854127971121 1106 6 -0.02591573514875023 1106 7 0.031006864456891492 1106 8 0.2667747477318727 1106 9 0.1794391805139668 1106 10-11 0.18679530630524255 1106 12-13 -0.11802468975359659 1106 14-15 0.21417918070819297 1106 16-17 -0.25566761203328525 1106 18-19 -0.21159290704255085 1106 20-21 0.10590412065955945 1106 22-23 0.1686979086376823 1106 24-25 0.019751864354816462 1106 26-27 0.11928928917338766 1106 28-29 0.08902371779146989 1106 30-31 0.01634998904872731 1106 32-33 0.0470403994229045 1106 34-35 -0.09359146661358864 1106 36-37 0.2993062006187941 1106 38-39 0.3745980285246091 1106 40-41 0.2550312378643227 1106 42-43 -0.1821458703337413 1106 44-45 -0.1363182909279672 1106 46-47 -0.014243363715628732 1106 48-49 -0.35693311520771687 1106 50-51 -0.1196279623798091 1106 52-53 -0.31731963055442947 1106 54-55 -0.07628927781694017 1106 56-57 0.2689973110009163 1106 58-59 -0.08324178259771031 1106 60-61 0.23340019344602325 1106 62-63 0.2363590934482218 1106 64-65 0.15248019206839203 1106 66-67 -0.15806712724512906 1106 68-69 0.33395558360008337 1106 70-71 0.26908482352531493 1106 72-73 0.2660759915032962 1106 74-75 0.6196268562949427 1106 76-77 1.229076291775705 1106 78-79 1.049024461958826 1106 80-81 1.1606066471117025 1106 82-83 0.8244131893175215 1106 84-85 0.4665209246955655 1106 86-87 0.5887271944719252 1106 88-89 0.6433703017253798 1106 90-91 0.3688206245520007 1106 92-93 0.341209036837963 1106 94-95 0.2182879934983717 1106 96-97 0.03722092333461191 1106 98-99 -0.1586507267318602 1106 100-101 1.0326598949308199 1107 1 0.38625628420874847 1107 2 0.48336325120337875 1107 3 0.6203157984567227 1107 4 0.3625147000494735 1107 5 0.2594746351858106 1107 6 0.376889792656776 1107 7 0.1212083296583586 1107 8 -0.02022991427278953 1107 9 0.46756771864249913 1107 10-11 0.16277765728759164 1107 12-13 0.2549773082483995 1107 14-15 0.5467216382506521 1107 16-17 0.6811039497382794 1107 18-19 0.260643190193548 1107 20-21 0.2694280966835336 1107 22-23 0.3610264134661847 1107 24-25 0.2206759402788876 1107 26-27 0.26466474704884746 1107 28-29 0.28576447703223096 1107 30-31 0.19854279624153293 1107 32-33 0.6416333065158071 1107 34-35 0.4990450635229422 1107 36-37 0.7544344057470056 1107 38-39 0.8895622309888083 1107 40-41 1.0843061296392165 1107 42-43 0.9956346824468127 1107 44-45 0.8261983590886786 1107 46-47 0.7540924671202092 1107 48-49 1.0475998518252538 1107 50-51 0.7147668095149626 1107 52-53 1.3118844986496967 1107 54-55 1.010977621949955 1107 56-57 0.8465031385067476 1107 58-59 1.3300324431765205 1107 60-61 1.6265278990737215 1107 62-63 1.2275970221861527 1107 64-65 0.9890394661276645 1107 66-67 0.8314017372237394 1107 68-69 0.3034860531305519 1107 70-71 0.2258988428393316 1107 72-73 0.7507787887060928 1107 74-75 0.8942688809369699 1107 76-77 0.6202684329678476 1107 78-79 0.1205570959914617 1107 80-81 0.6389408129458687 1107 82-83 0.49368142108575697 1107 84-85 0.3454753036499447 1107 86-87 0.7693174375621687 1107 88-89 0.24583034168541573 1107 90-91 0.4661191593505336 1107 92-93 -0.03187621124728324 1107 94-95 0.9860618862722674 1107 96-97 1.1607432343569215 1107 98-99 0.689022433441302 1107 100-101 1.819123431394356 1108 1 0.05816770612016953 1108 2 -0.13027311243298811 1108 3 -0.1617354835945619 1108 4 0.05598789352266209 1108 5 0.058425684136857114 1108 6 0.005677671444658472 1108 7 -0.2377660293159991 1108 8 -0.2364303804732586 1108 9 0.2939080449828282 1108 10-11 -0.014669895159961754 1108 12-13 -0.0658268875558008 1108 14-15 -0.22309188156287263 1108 16-17 -0.14028299664866495 1108 18-19 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2316 68-69 -1.1003094267518811 2316 70-71 -1.0237464394146443 2316 72-73 -0.6225109650184422 2316 74-75 -0.24683277103424928 2316 76-77 -0.0395572485969673 2316 78-79 -0.45550452354841653 2316 80-81 -0.8431459408800137 2316 82-83 -0.585395299295314 2316 84-85 -0.9180546446626145 2316 86-87 -0.9577272568738273 2316 88-89 -1.806034460179383 2316 90-91 -1.7927994582637368 2316 92-93 -1.4582216049839793 2316 94-95 -1.8668569340378554 2316 96-97 -1.4178774203506883 2316 98-99 -2.440094826110741 2316 100-101 -3.0988100843652475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 11.0 6 31.0 7 56.0 8 94.0 9 181.0 10 310.0 11 484.0 12 710.0 13 918.0 14 1212.0 15 1719.0 16 2151.0 17 2981.0 18 2582.0 19 1637.0 20 1475.0 21 1491.0 22 1553.0 23 1647.0 24 1899.0 25 2000.0 26 2322.0 27 2608.0 28 3187.0 29 3898.0 30 4586.0 31 5626.0 32 7022.0 33 9055.0 34 12169.0 35 16402.0 36 23417.0 37 36890.0 38 25677.0 39 1155.0 >>END_MODULE >>Per base sequence content fail #Base G 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16.71243657797351 30.729751000519094 34.79210971382642 17.765702707680973 44-45 17.006176198888674 31.29041530960534 34.46726447136705 17.23614402013893 46-47 17.657417795564783 31.132749487879348 34.3832504451403 16.826582271415575 48-49 17.470200634640666 31.340755149827103 33.76349727751993 17.425546938012296 50-51 17.302856504932947 31.125996008986757 33.37045254741072 18.20069493866957 52-53 17.340377434317386 31.778551773025892 33.715958628465536 17.165112164191186 54-55 18.2300998565504 31.34038859771039 32.90856622961983 17.52094531611938 56-57 18.25745016940449 31.645707396306033 31.990377155232565 18.106465279056916 58-59 17.480757101313372 31.274245494175503 33.27053924769894 17.974458156812183 60-61 18.133180765259134 32.17074600206525 32.07334431079233 17.622728921883287 62-63 17.606903442232234 32.06126470079316 33.2113732647901 17.12045859218451 64-65 17.709606657847587 31.638451190854948 32.72297482096708 17.92896733033038 66-67 17.584576656228894 31.971399387129722 32.09447579497313 18.34954816166826 68-69 17.356661233785083 31.431545692022596 32.68213177342651 18.529661300765813 70-71 17.926884745374828 31.347349090594484 32.86390872733463 17.861857436696056 72-73 17.724825640295723 30.89941419604757 32.368529015296765 19.007231148359942 74-75 17.37937617218898 31.89665758685362 31.73310931499509 18.99085692596231 76-77 17.142227208537765 31.36634348643927 32.2976495476035 19.19377975741947 78-79 17.365312911652413 30.651220165665677 32.20656857710599 19.776898345575923 80-81 17.975417659765675 31.941692296528668 30.909334479467272 19.17355556423839 82-83 18.186613379922022 30.851239000538634 31.365872854180566 19.59627476535878 84-85 17.120609536568008 30.981426438369546 31.25465734499937 20.643306680063077 86-87 17.181722023605193 31.29962518594182 31.284833566371113 20.233819224081874 88-89 17.682816211132106 30.542538731834206 31.5539901255104 20.22065493152329 90-91 17.122794482928 29.660960184869918 32.720914525573114 20.49533080662897 92-93 17.79919288668598 30.795894104053982 31.256942235022912 20.147970774237123 94-95 17.473500895862287 29.744581568121813 31.877068716265622 20.904848819750274 96-97 17.555977907460278 29.908933859133548 31.375536893927343 21.15955133947883 98-99 16.943291646548907 30.869338304861472 31.19252484601194 20.99484520257768 100-101 16.515026346342772 30.971186478521034 30.9524872733768 21.5612999017594 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2195.0 1 1396.5 2 529.0 3 415.0 4 320.0 5 299.0 6 387.0 7 588.0 8 780.5 9 809.0 10 643.0 11 510.5 12 543.5 13 577.5 14 600.5 15 598.0 16 591.5 17 621.5 18 665.0 19 671.0 20 722.5 21 767.5 22 812.0 23 904.0 24 1000.5 25 1221.5 26 1631.0 27 2040.0 28 2148.0 29 2453.0 30 3335.5 31 4111.5 32 5388.0 33 7014.5 34 8098.0 35 9500.5 36 11494.0 37 12601.5 38 12189.0 39 10984.5 40 9518.5 41 8066.5 42 6451.5 43 6028.5 44 6258.5 45 5859.0 46 4737.0 47 4065.5 48 4331.0 49 3623.0 50 2411.5 51 1533.0 52 1033.0 53 789.5 54 615.0 55 503.0 56 408.5 57 355.0 58 356.0 59 331.0 60 280.0 61 231.5 62 143.0 63 70.0 64 34.5 65 23.5 66 15.0 67 8.0 68 6.0 69 5.0 70 2.5 71 1.0 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020652277053087517 2 0.005023526850751018 3 0.0011163393001668927 4 0.0 5 0.003349017900500678 6 0.0 7 5.581696500834463E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.009209799226376865 14-15 0.003349017900500678 16-17 0.0 18-19 5.581696500834463E-4 20-21 0.0011163393001668927 22-23 0.001395424125208616 24-25 0.0011163393001668927 26-27 0.0 28-29 5.581696500834463E-4 30-31 0.0 32-33 2.7908482504172317E-4 34-35 0.0 36-37 0.0019535937752920623 38-39 0.014512410902169605 40-41 8.372544751251695E-4 42-43 0.0 44-45 8.372544751251695E-4 46-47 0.0 48-49 2.7908482504172317E-4 50-51 0.002511763425375509 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0011163393001668927 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.581696500834463E-4 70-71 8.372544751251695E-4 72-73 8.372544751251695E-4 74-75 0.002790848250417232 76-77 0.0 78-79 5.581696500834463E-4 80-81 0.0022326786003337854 82-83 2.7908482504172317E-4 84-85 0.002511763425375509 86-87 8.372544751251695E-4 88-89 0.004744442025709294 90-91 0.0022326786003337854 92-93 0.0 94-95 0.0 96-97 8.372544751251695E-4 98-99 0.0019535937752920623 100-101 0.002790848250417232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 179157.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.174411270561574 #Duplication Level Percentage of deduplicated Percentage of total 1 74.94642993801179 21.865179702718844 2 9.206397795974592 5.371824712403088 3 3.5547562562179538 3.11123762956513 4 2.0414020050508914 2.382268066556149 5 1.45404453968011 2.1210446703170964 6 1.0293104767735517 1.8017716304693647 7 0.8705135073084871 1.7777703355157766 8 0.684931506849315 1.5985978778389902 9 0.5586592178770949 1.466869840419297 >10 4.779214815948573 29.033194349090465 >50 0.6141424963648887 12.479557036565694 >100 0.2506313614448611 11.45364121971232 >500 0.0 0.0 >1k 0.009566082497895461 5.537042928827788 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2498 1.3943077859084492 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2452 1.3686319820046104 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1949 1.0878726480126368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1847 1.0309393437041254 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1174 0.655291169197966 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 470 0.2623397355392198 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 406 0.22661687793387922 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 386 0.2154534849322103 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 338 0.18866134172820487 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 309 0.17247442187578493 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 296 0.16521821642470014 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 294 0.16410187712453322 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 294 0.16410187712453322 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 279 0.15572933237328151 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 271 0.15126397517261395 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 255 0.14233326077127884 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 254 0.14177509112119538 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 230 0.12837901951919264 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 223 0.12447183196860855 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 217 0.12112281406810785 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 214 0.11944830511785752 No Hit CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC 213 0.11889013546777408 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 196 0.1094012514163555 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 195 0.10884308176627204 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 194 0.1082849121161886 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 191 0.10661040316593826 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 189 0.10549406386577136 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 189 0.10549406386577136 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 186 0.10381955491552103 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 185 0.10326138526543759 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 180 0.10047053701502034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.7908482504172317E-4 0.0 22-23 0.0 0.0 0.0 0.0013954241252086158 0.0 24-25 0.0 0.0 0.0 0.004744442025709294 0.0 26-27 0.0 0.0 0.0 0.006698035801001356 0.0 28-29 0.0 0.0 0.0 0.015349665377294774 0.0 30-31 0.0 0.0 0.0 0.03516468795525712 0.0 32-33 0.0 0.0 0.0 0.06084049185909565 0.0 34-35 0.0 0.0 0.0 0.07981825996193283 5.581696500834463E-4 36-37 0.0 0.0 0.0 0.10186596114022896 5.581696500834463E-4 38-39 0.0 0.0 0.0 0.12363457749348336 5.581696500834463E-4 40-41 0.0 0.0 0.0 0.14568227867177952 5.581696500834463E-4 42-43 0.0 0.0 0.0 0.2093136187812924 5.581696500834463E-4 44-45 0.0 0.0 0.0 0.283271097417349 5.581696500834463E-4 46-47 0.0 0.0 0.0 0.33936714725073536 5.581696500834463E-4 48-49 0.0 0.0 0.0 0.41192920176158343 5.581696500834463E-4 50-51 0.0 0.0 0.0 0.505143533325519 5.581696500834463E-4 52-53 0.0 0.0 0.0 0.6700826649251774 5.581696500834463E-4 54-55 0.0 0.0 0.0 0.8057178898954549 5.581696500834463E-4 56-57 0.0 0.0 0.0 1.0379164643301686 5.581696500834463E-4 58-59 0.0 0.0 0.0 1.3513287228520237 5.581696500834463E-4 60-61 0.0 0.0 0.0 1.6237155120927453 5.581696500834463E-4 62-63 0.0 0.0 0.0 1.9658735075938982 5.581696500834463E-4 64-65 0.0 0.0 0.0 2.2516563684366226 5.581696500834463E-4 66-67 0.0 0.0 0.0 2.706006463604548 5.581696500834463E-4 68-69 0.0 0.0 0.0 3.209475487979817 5.581696500834463E-4 70-71 0.0 0.0 0.0 3.6378706944188615 5.581696500834463E-4 72-73 0.0 0.0 0.0 4.19966844722785 5.581696500834463E-4 74-75 0.0 0.0 0.0 4.7779322047143005 8.372544751251695E-4 76-77 0.0 0.0 0.0 5.180651607249508 0.0011163393001668927 78-79 0.0 0.0 0.0 5.623559224590722 0.0011163393001668927 80-81 0.0 0.0 0.0 6.321829456845114 0.0011163393001668927 82-83 0.0 0.0 0.0 6.883069040004019 0.0011163393001668927 84-85 0.0 0.0 0.0 7.718369921353896 0.0011163393001668927 86-87 0.0 0.0 0.0 8.70214392962597 0.0011163393001668927 88-89 0.0 0.0 0.0 9.958304727138767 0.0011163393001668927 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAGG 25 0.005015194 56.997654 1 GGATGAC 100 6.2937033E-10 42.760178 8 GGGATGA 90 1.184344E-8 42.232273 7 GATGACC 70 4.282794E-6 40.723976 9 CCCTTGG 85 3.6110578E-7 39.126957 8 CATGGGA 355 0.0 36.125275 4 ACCCTTG 95 8.629431E-7 35.00833 7 ATGGGAT 195 0.0 34.101162 5 CCTTGGC 100 1.2886921E-6 33.257915 9 AACCCTT 105 1.8863393E-6 31.674204 6 CAATGGG 90 2.397786E-5 31.674204 9 GAAGGTA 160 1.877197E-9 29.694569 9 GTACATG 645 0.0 29.456154 1 GGGAAGC 65 0.0058243037 29.237726 7 TTAACAG 65 0.0058243037 29.237726 9 ACATGGG 620 0.0 29.111706 3 GGACAAT 105 6.862748E-5 27.149317 6 GGTTAAC 70 0.008363285 27.149317 7 AGAAGGT 160 6.16601E-8 26.725111 8 GGTATCA 1410 0.0 26.612381 1 >>END_MODULE