FastQCFastQC Report
Thu 26 May 2016
SRR937115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4467024
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121130.27116487397426114No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT111600.24983075980787212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83250.18636568775990459No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68980.15442048218232093No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55020.12316925093753693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53730.12028142226233841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG165600.023.6233981
GTATCAA282350.023.4175911
TACATGG165900.022.97952
GAGTACT121600.021.64584712-13
ACATGGG168950.021.4923783
AGAGTAC202200.021.27939210-11
GTACTTT124450.020.61635214-15
TATCAAC320500.020.4674472
TCAACGC319300.020.4374074
CAACGCA318700.020.4283965
ATCAACG320800.020.3712483
AACGCAG325500.020.1181986
AGTACTT128050.019.46096212-13
TACCGTA3105.5642886E-918.394837
ACGCAGA355150.018.157017
CGCAGAG358000.017.9209528
GGTATCA217400.017.8853151
CATGGGG101300.017.8759754
CATGGGA74950.017.3119184
GCAGAGT367850.016.795499
Produced by FastQC (version 0.11.5)