##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937115_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4467024 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5225875213565 31.0 31.0 34.0 30.0 34.0 2 31.62059706865242 33.0 31.0 34.0 30.0 34.0 3 31.634097108052252 33.0 31.0 34.0 28.0 34.0 4 35.21252404285269 37.0 35.0 37.0 33.0 37.0 5 35.22965222483694 37.0 35.0 37.0 33.0 37.0 6 35.27314964056607 37.0 35.0 37.0 32.0 37.0 7 35.198797902137976 37.0 35.0 37.0 32.0 37.0 8 35.16278041040299 37.0 35.0 37.0 32.0 37.0 9 36.742612531296004 39.0 37.0 39.0 32.0 39.0 10-11 36.60545477257342 39.0 37.0 39.0 32.0 39.0 12-13 36.495974165350354 39.0 37.0 39.0 32.0 39.0 14-15 37.5994399179409 40.0 37.0 41.0 32.0 41.0 16-17 37.52301756158015 40.0 37.0 41.0 32.0 41.0 18-19 37.46068512280212 40.0 37.0 41.0 32.0 41.0 20-21 37.38369258817504 40.0 37.0 41.0 32.0 41.0 22-23 37.23919302873681 40.0 37.0 41.0 31.0 41.0 24-25 37.060108362972755 39.0 36.0 41.0 31.0 41.0 26-27 37.01158455830996 39.0 36.0 41.0 31.0 41.0 28-29 37.094030611879404 39.0 37.0 41.0 31.0 41.0 30-31 36.977442252381 39.0 36.0 41.0 30.0 41.0 32-33 36.805201068989106 39.0 36.0 41.0 30.0 41.0 34-35 36.61640635913306 39.0 36.0 41.0 30.0 41.0 36-37 36.46587034231291 39.0 35.0 41.0 30.0 41.0 38-39 36.301831040083954 39.0 35.0 41.0 30.0 41.0 40-41 36.10903590399335 39.0 35.0 40.5 29.0 41.0 42-43 35.912592029951035 39.0 35.0 40.0 28.5 41.0 44-45 35.6849937452765 38.5 35.0 40.0 27.5 41.0 46-47 35.383696841566106 38.0 34.5 40.0 26.5 41.0 48-49 35.06934952666474 38.0 34.0 40.0 26.0 41.0 50-51 34.420451065407306 37.5 33.5 39.5 24.5 40.5 52-53 34.74935617090931 38.0 34.0 40.0 26.0 40.5 54-55 35.06811492394041 38.0 34.0 40.0 26.0 41.0 56-57 34.92896713785285 38.0 34.0 40.0 26.0 41.0 58-59 34.66947289739208 38.0 34.0 40.0 25.5 41.0 60-61 34.35490272718481 38.0 34.0 40.0 24.5 41.0 62-63 34.03587220485048 37.0 33.5 40.0 23.5 41.0 64-65 33.71582288342306 37.0 33.0 39.5 23.0 41.0 66-67 33.31549248448184 36.0 33.0 39.0 22.0 41.0 68-69 32.85940359845839 36.0 32.0 39.0 22.0 40.5 70-71 32.49133315603409 35.0 32.0 38.5 20.5 40.0 72-73 32.10280905139529 35.0 31.5 37.5 20.0 40.0 74-75 31.678230965403365 35.0 31.0 37.0 19.0 39.0 76-77 31.247804130893407 35.0 31.0 36.5 18.0 39.0 78-79 30.82846890905444 35.0 31.0 36.0 15.0 38.5 80-81 30.410576034514254 34.0 30.0 35.5 11.0 37.0 82-83 30.005599813208974 34.0 30.0 35.0 7.5 37.0 84-85 29.655595873225664 34.0 29.5 35.0 7.0 36.5 86-87 29.347551971961646 34.0 29.0 35.0 2.0 36.0 88-89 28.96811960267059 34.0 29.0 35.0 2.0 36.0 90-91 28.623395576115108 34.0 29.0 35.0 2.0 35.0 92-93 28.254089859378414 34.0 29.0 35.0 2.0 35.0 94-95 27.84301987184309 34.0 27.0 35.0 2.0 35.0 96-97 27.38186340615139 34.0 27.0 35.0 2.0 35.0 98-99 26.76373386397745 33.0 25.5 35.0 2.0 35.0 100-101 24.594260742722675 31.0 20.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4954397390876224 1101 2 0.46075890924174345 1101 3 0.5700392352923664 1101 4 0.3631525128332882 1101 5 0.34718251338246375 1101 6 0.2659985929231752 1101 7 0.42425836005810424 1101 8 0.3987340931276222 1101 9 0.6119433543422232 1101 10-11 0.6063172486879722 1101 12-13 0.6850705864715607 1101 14-15 0.9787859203909122 1101 16-17 0.8803210588973442 1101 18-19 0.8695807632849579 1101 20-21 0.8499820130948308 1101 22-23 0.9121497740264886 1101 24-25 0.994186861409986 1101 26-27 0.9644055096299553 1101 28-29 0.9459590486222993 1101 30-31 0.8343714404847518 1101 32-33 0.8412094894333819 1101 34-35 0.8932229581237294 1101 36-37 0.9074945465844166 1101 38-39 0.9281604510362342 1101 40-41 0.9550672800162658 1101 42-43 1.0268628390175252 1101 44-45 1.0018945039216334 1101 46-47 0.9547376118163342 1101 48-49 0.8771956528144216 1101 50-51 0.84122744387507 1101 52-53 1.0803593754796026 1101 54-55 1.1055906113404887 1101 56-57 1.0946429916331653 1101 58-59 1.0177358965717076 1101 60-61 1.0199568043662666 1101 62-63 1.131826511908109 1101 64-65 0.8882246581887756 1101 66-67 0.8412625489113026 1101 68-69 0.9434369834109546 1101 70-71 0.9068622359642902 1101 72-73 0.9805861987696218 1101 74-75 0.823726534686049 1101 76-77 0.8499802404747605 1101 78-79 0.901958640233218 1101 80-81 0.9687254665390626 1101 82-83 0.7996064440907205 1101 84-85 1.0144031408408622 1101 86-87 1.135196073600408 1101 88-89 1.2312518281252096 1101 90-91 1.1812966207681903 1101 92-93 1.1205026957170325 1101 94-95 1.2259069200581116 1101 96-97 1.3304512350998579 1101 98-99 1.3802758312842478 1101 100-101 1.5820225072106133 1102 1 0.31927978871384965 1102 2 0.4357391360158225 1102 3 0.3765889554073887 1102 4 0.29021476729143103 1102 5 0.2583715066792678 1102 6 0.2675021725759805 1102 7 0.23415890728061584 1102 8 0.2512455294595952 1102 9 0.31878014669682386 1102 10-11 0.42145866384999664 1102 12-13 0.45751400686872756 1102 14-15 0.5881960167662683 1102 16-17 0.5257997426663934 1102 18-19 0.49587760183615615 1102 20-21 0.49954290769654364 1102 22-23 0.47980892125397645 1102 24-25 0.5922660683412815 1102 26-27 0.49357474356907005 1102 28-29 0.6751125872537855 1102 30-31 0.5839661691507487 1102 32-33 0.638831309124896 1102 34-35 0.4117306755703325 1102 36-37 0.5109343944830229 1102 38-39 0.5641829871205886 1102 40-41 0.5547464617646227 1102 42-43 0.5464183778431533 1102 44-45 0.5878614020115407 1102 46-47 0.3500932536190362 1102 48-49 0.49903722530373784 1102 50-51 0.39391483054911447 1102 52-53 0.5345367377389678 1102 54-55 0.6036858437752315 1102 56-57 0.7186570104265897 1102 58-59 0.5287527918109234 1102 60-61 0.6359008815229004 1102 62-63 0.6929514582237672 1102 64-65 0.8177736173125254 1102 66-67 0.5228416069242883 1102 68-69 0.47306199763688994 1102 70-71 0.5450392336874756 1102 72-73 0.5918547252311868 1102 74-75 0.579470301854851 1102 76-77 0.640587182203344 1102 78-79 0.7135998477020706 1102 80-81 0.6678978857671147 1102 82-83 0.5679113882887279 1102 84-85 0.7299172370101736 1102 86-87 0.8264693284660076 1102 88-89 0.6651322298810598 1102 90-91 0.5850314435928219 1102 92-93 0.6872396500656954 1102 94-95 0.7741228830885056 1102 96-97 0.5943442129135583 1102 98-99 0.6743527567826071 1102 100-101 1.252058778119988 1103 1 0.2821272227568521 1103 2 0.36688372919842394 1103 3 0.27516142721044545 1103 4 0.2823333076368115 1103 5 0.2513612459206911 1103 6 0.21140384424960956 1103 7 0.3042649679448104 1103 8 0.16268979869228417 1103 9 0.37876139775992357 1103 10-11 0.3600152898598523 1103 12-13 0.15077155152534516 1103 14-15 0.2430702927388495 1103 16-17 0.49957527466428076 1103 18-19 0.5720071101817368 1103 20-21 0.5355360066553843 1103 22-23 0.4141747889806169 1103 24-25 0.6278708878156252 1103 26-27 0.5998476441933107 1103 28-29 0.6311070674439847 1103 30-31 0.6736382673818753 1103 32-33 0.6164098314886957 1103 34-35 0.5272114655835125 1103 36-37 0.6709408402294059 1103 38-39 0.673405903795242 1103 40-41 0.8087794655208143 1103 42-43 0.7775315096446036 1103 44-45 0.8238184354846609 1103 46-47 0.7126726904091569 1103 48-49 0.7759553783927728 1103 50-51 0.6206964591487889 1103 52-53 0.7176747163580259 1103 54-55 0.7372689497808977 1103 56-57 0.6963276788274655 1103 58-59 0.7292635276875146 1103 60-61 0.7651427645505109 1103 62-63 0.7781027733990271 1103 64-65 0.863861854976399 1103 66-67 0.8279367520239163 1103 68-69 0.8425224605284427 1103 70-71 0.8226265805850161 1103 72-73 0.8297963835529103 1103 74-75 0.8903435342304 1103 76-77 0.6939176123929691 1103 78-79 0.862921253846757 1103 80-81 0.8095895233064212 1103 82-83 0.8118553282000498 1103 84-85 0.8965202775096657 1103 86-87 0.9111867707324564 1103 88-89 0.8967039177074199 1103 90-91 0.8103947522046688 1103 92-93 0.8194992039023852 1103 94-95 0.8107698927865421 1103 96-97 0.7852030830407202 1103 98-99 1.010790663398577 1103 100-101 1.289928433362082 1104 1 0.38936121828130865 1104 2 0.385560942395049 1104 3 0.381876655113377 1104 4 0.39053778002363515 1104 5 0.39804717421392866 1104 6 0.3543994842084288 1104 7 0.3910741606765811 1104 8 0.32801803990967215 1104 9 0.33537786620135535 1104 10-11 0.44865658070460057 1104 12-13 0.46708410507025633 1104 14-15 0.529265739250441 1104 16-17 0.5979550295147931 1104 18-19 0.5019905159331302 1104 20-21 0.4609336827958046 1104 22-23 0.5632157378026861 1104 24-25 0.4759863977459915 1104 26-27 0.5914484781436329 1104 28-29 0.49364868809630025 1104 30-31 0.5474198531671632 1104 32-33 0.5837819406866629 1104 34-35 0.6561951447203782 1104 36-37 0.7297706922605585 1104 38-39 0.681671742494494 1104 40-41 0.6826026823454043 1104 42-43 0.741205228659183 1104 44-45 0.6299868093563177 1104 46-47 0.5671375986006666 1104 48-49 0.558826324663805 1104 50-51 0.5654188316360802 1104 52-53 0.8098894211723504 1104 54-55 0.7528748559041532 1104 56-57 0.8577848150606755 1104 58-59 0.9332930549799414 1104 60-61 0.7909204797293867 1104 62-63 0.7772957045981244 1104 64-65 0.7992468257557306 1104 66-67 0.6909105959316193 1104 68-69 0.8065115699815166 1104 70-71 0.7136715446003379 1104 72-73 0.7192503521709241 1104 74-75 0.6890108839452616 1104 76-77 0.7058422045375856 1104 78-79 0.7862792583488947 1104 80-81 0.794697493946984 1104 82-83 0.7685002154347771 1104 84-85 0.8816959712980008 1104 86-87 0.7888251690093817 1104 88-89 0.7425871812817597 1104 90-91 0.806727149047358 1104 92-93 0.602637386545485 1104 94-95 0.5476915973490897 1104 96-97 0.5141140948245493 1104 98-99 0.7219952712316946 1104 100-101 1.0104746392454338 1105 1 0.2760058373009784 1105 2 0.26971485453555033 1105 3 0.2624656960031082 1105 4 0.26490569731109304 1105 5 0.2433919602840291 1105 6 0.2114058200851403 1105 7 0.2365656802335252 1105 8 0.18765052896109324 1105 9 0.2955058629506411 1105 10-11 0.3573281535370896 1105 12-13 0.32679466386147027 1105 14-15 0.34900459126783545 1105 16-17 0.4409661887845857 1105 18-19 0.3787673079628675 1105 20-21 0.444018886040503 1105 22-23 0.4140188214633511 1105 24-25 0.5041611084176552 1105 26-27 0.4623389999128591 1105 28-29 0.4276016882317464 1105 30-31 0.2659586886300147 1105 32-33 0.33814538072157063 1105 34-35 0.20714364502889993 1105 36-37 0.23674147348469177 1105 38-39 0.37950234432753405 1105 40-41 0.49541590190384 1105 42-43 0.418104674834332 1105 44-45 0.2956188168209195 1105 46-47 0.2672453912775339 1105 48-49 0.40989183552208175 1105 50-51 0.1288236881382545 1105 52-53 0.28497858998358083 1105 54-55 0.33934320661975903 1105 56-57 0.41944297870990255 1105 58-59 0.41195273863759496 1105 60-61 0.3918881682631081 1105 62-63 0.3108948512864629 1105 64-65 0.43361613513012287 1105 66-67 0.5593960053556728 1105 68-69 0.6038942584399862 1105 70-71 0.5688361870973679 1105 72-73 0.4497743035908499 1105 74-75 0.5512620013772 1105 76-77 0.36130163084727585 1105 78-79 0.36810387044575066 1105 80-81 0.4571634442532435 1105 82-83 0.49535018608807846 1105 84-85 0.6190740696320098 1105 86-87 0.7649094918531496 1105 88-89 0.6773067698260107 1105 90-91 0.6147799956770044 1105 92-93 0.5880383452042643 1105 94-95 0.6434681184862363 1105 96-97 0.5710927770825904 1105 98-99 0.7003276263417817 1105 100-101 0.9133257837666342 1106 1 0.4344235827316112 1106 2 0.42173498317446345 1106 3 0.4950274259445173 1106 4 0.3439769063098055 1106 5 0.4844761296663975 1106 6 0.47743344876395355 1106 7 0.5503510441519026 1106 8 0.4730304056663641 1106 9 0.6504476447677305 1106 10-11 0.6594497010192129 1106 12-13 0.6380410542190802 1106 14-15 0.7814766994622886 1106 16-17 0.8124889679893315 1106 18-19 0.8081685526748217 1106 20-21 0.8166285939327054 1106 22-23 0.9348280781432976 1106 24-25 0.8335640092693453 1106 26-27 0.9516620247273053 1106 28-29 0.8090581281380835 1106 30-31 0.715382593230423 1106 32-33 0.8315447824329709 1106 34-35 0.7526841014146228 1106 36-37 0.6651405912864234 1106 38-39 0.8079081191615529 1106 40-41 0.6403233974531446 1106 42-43 0.6842689002489664 1106 44-45 0.7062051775034703 1106 46-47 0.6156736033257673 1106 48-49 0.7181999231622171 1106 50-51 0.7275265317626634 1106 52-53 0.7486092045756507 1106 54-55 0.6785460244510659 1106 56-57 0.7397614659798037 1106 58-59 0.8984768640937517 1106 60-61 0.8776958393168286 1106 62-63 0.8598273091759765 1106 64-65 0.8531550444864848 1106 66-67 0.9282799323200024 1106 68-69 0.7833918097654688 1106 70-71 0.8040375811124036 1106 72-73 0.8866518594737371 1106 74-75 0.8267637699674317 1106 76-77 0.9392812025259296 1106 78-79 1.0713994308036519 1106 80-81 1.1453704581952522 1106 82-83 1.2668566219306143 1106 84-85 1.4555477361750917 1106 86-87 1.4199615159806491 1106 88-89 1.3486476241677323 1106 90-91 1.2398112396578433 1106 92-93 1.1814491033024659 1106 94-95 1.227056947327302 1106 96-97 1.2684135001875276 1106 98-99 1.4786184827061284 1106 100-101 1.5466756506631008 1107 1 0.20885367916208608 1107 2 0.3233923630787636 1107 3 0.31552188755904 1107 4 0.3074115824121719 1107 5 0.21325351357481992 1107 6 0.27322740570287607 1107 7 0.37600523804712793 1107 8 0.35995561582296887 1107 9 0.3494073980105412 1107 10-11 0.4086364348324949 1107 12-13 0.4167813749776812 1107 14-15 0.3066894643261904 1107 16-17 0.43440024007676215 1107 18-19 0.3821962141966182 1107 20-21 0.5220780730071581 1107 22-23 0.5346851669414434 1107 24-25 0.4528040782809697 1107 26-27 0.44167851100813493 1107 28-29 0.3809797563881645 1107 30-31 0.5409184309810584 1107 32-33 0.5182744887973385 1107 34-35 0.5790891651362458 1107 36-37 0.5135473369718255 1107 38-39 0.5936007105952825 1107 40-41 0.7268695269046006 1107 42-43 0.7822562117401404 1107 44-45 0.757772767171943 1107 46-47 0.7633434283018161 1107 48-49 0.7944607883667629 1107 50-51 0.6050847224930109 1107 52-53 0.6404613495069711 1107 54-55 0.768305782612579 1107 56-57 0.7207150130183493 1107 58-59 0.7025371139039933 1107 60-61 0.6789429931733793 1107 62-63 0.6966590736979796 1107 64-65 0.7887995633433036 1107 66-67 0.8579149467557059 1107 68-69 0.8305421790376073 1107 70-71 0.8545373716354803 1107 72-73 0.6590344522437661 1107 74-75 0.703118753284695 1107 76-77 0.7027250483474532 1107 78-79 0.7029674643076618 1107 80-81 0.7580281194835976 1107 82-83 0.8408272669168468 1107 84-85 0.8497872744093655 1107 86-87 0.8332133308789373 1107 88-89 0.8815527939007239 1107 90-91 0.8186324211038638 1107 92-93 1.0367787707975928 1107 94-95 0.9425055512195435 1107 96-97 0.9598175057168348 1107 98-99 1.011178094766965 1107 100-101 1.0954684035780282 1108 1 0.003308183653668806 1108 2 0.12851367712156048 1108 3 0.0620200910952633 1108 4 0.061869749023429677 1108 5 0.0830503081231555 1108 6 0.05923549274319129 1108 7 -0.0035428361335760883 1108 8 -0.11322568610276562 1108 9 -0.07802432539647697 1108 10-11 -0.023786445483693797 1108 12-13 -0.23807890756244632 1108 14-15 0.23230801659071432 1108 16-17 0.13066243096938734 1108 18-19 0.17454327007178705 1108 20-21 0.1618288210415102 1108 22-23 0.18585354344109106 1108 24-25 0.18936650392053167 1108 26-27 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-1.0913166987556906 2316 64-65 -1.2815701986805124 2316 66-67 -1.2991981663181065 2316 68-69 -0.8538789805945441 2316 70-71 -1.3728995086127966 2316 72-73 -1.1467587433667426 2316 74-75 -1.4693179094983897 2316 76-77 -1.863782397237177 2316 78-79 -2.075852686978827 2316 80-81 -1.9327162734748384 2316 82-83 -1.7901055394194358 2316 84-85 -1.571201031736976 2316 86-87 -1.4154146658586981 2316 88-89 -1.8212315206624758 2316 90-91 -1.7474092063517155 2316 92-93 -2.165079392248952 2316 94-95 -2.2631622939929663 2316 96-97 -2.2077101210220675 2316 98-99 -2.338668207381861 2316 100-101 -2.167127064601427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13084.0 3 4216.0 4 4406.0 5 4779.0 6 5398.0 7 6185.0 8 7645.0 9 9432.0 10 11523.0 11 13941.0 12 16673.0 13 19131.0 14 21230.0 15 24610.0 16 29340.0 17 35959.0 18 32501.0 19 25726.0 20 25954.0 21 28163.0 22 31032.0 23 35131.0 24 40498.0 25 46803.0 26 54412.0 27 64833.0 28 77726.0 29 93149.0 30 113606.0 31 139758.0 32 175304.0 33 225358.0 34 302326.0 35 421730.0 36 620478.0 37 862475.0 38 706648.0 39 115822.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.815307947763046 18.44958344028997 14.507441718151448 23.22766689379553 2 15.30750577330981 20.091947213148405 40.187165931175606 24.413381082366183 3 19.599400000537635 24.209767733183035 29.315072298322313 26.87575996795702 4 13.015268741186778 16.541252095504106 37.30536384048101 33.138115322828114 5 14.281946314379809 36.943867279308286 33.87192700648796 14.902259399823953 6 31.974553180370656 35.63637105172165 17.72582663060005 14.663249137307638 7 28.557844314490627 32.2861129450765 20.91036548278411 18.245677257648758 8 26.186283745447795 33.98277177191443 20.067466246709373 19.763478235928396 9 26.10979387901332 17.16256925180594 20.46623815471659 36.26139871446415 10-11 24.892645480039395 26.22728937722781 27.438384393891667 21.441680748841126 12-13 26.08305088372659 23.612690026788695 27.68799727441218 22.61626181507254 14-15 23.13793494418544 24.98301520072313 26.08250086156403 25.796548993527402 16-17 22.422293565820183 28.108422646984437 26.52058248856684 22.948701298628535 18-19 22.379145598429066 27.208033829575424 28.460465728016793 21.952354843978718 20-21 23.366523350550384 26.04594927362207 28.32167135027797 22.265856025549578 22-23 22.97806966449208 25.961964119291704 28.29082729944799 22.769138916768227 24-25 22.979103542148575 26.1443222346183 28.273437352123004 22.60313687111012 26-27 22.714984069032326 26.146004938879962 28.51805602996926 22.620954962118457 28-29 22.48495266517346 26.68540398721191 28.263037392614443 22.56660595500019 30-31 22.83485121239015 26.139047613714943 28.13333072032297 22.892770453571938 32-33 22.664777371551207 26.228169676858194 28.25680273346795 22.850250218122653 34-35 23.12085954499354 26.249618282229157 28.044058545399697 22.58546362737761 36-37 23.060101762619958 26.0383857964602 28.235437065805264 22.666075375114577 38-39 22.7468879129021 26.45911804171728 28.110681650047294 22.68331239533333 40-41 22.938323737794544 26.367752090239993 27.981567784436894 22.712356387528573 42-43 22.717942624003804 26.34676275962004 27.7476802854475 23.187614330928657 44-45 22.93590361812775 26.22873291953567 27.726418270108354 23.108945192228227 46-47 23.320892472398285 26.567101708629004 27.44347474007327 22.668531078899438 48-49 23.144370671672647 26.52608183268228 27.406674308217895 22.922873187427186 50-51 22.848895422985937 26.72255357349387 27.564495937965937 22.864055065554258 52-53 23.1699757209855 26.828803721602725 26.97153055435926 23.029690003052515 54-55 22.99149252110072 26.588228838528117 27.156976815804178 23.263301824566984 56-57 23.079507436848882 26.7836084938676 27.020565871104772 23.116318198178746 58-59 23.411361311439865 26.523031752726585 27.0731646129314 22.99244232290215 60-61 23.123637478668343 26.644360122017403 27.468362851035778 22.763639548278473 62-63 22.82528859475327 26.700673453090683 27.56156893402221 22.91246901813384 64-65 22.866722103289042 26.869401515153214 27.150074611004893 23.113801770552854 66-67 22.929860044446905 26.72732400385914 26.935838267211416 23.406977684482538 68-69 22.842407594309556 26.66488375457991 27.264701521254057 23.228007129856476 70-71 23.174666547392185 26.532541837409994 27.033662610774407 23.259129004423418 72-73 23.049545107690985 26.49225993436797 27.201915222905292 23.256279735035754 74-75 22.863153435011345 27.110025452905074 26.875067808541036 23.151753303542545 76-77 23.052217568929947 26.698071257073114 26.82359032921965 23.426120844777284 78-79 22.95701941546405 26.80130369773029 26.950344124680097 23.291332762125567 80-81 23.144251986631794 26.739549957325742 26.81438387931975 23.301814176722722 82-83 23.123856289928003 26.671719271963408 27.09690866346345 23.107515774645144 84-85 22.98519612496749 26.851313200732317 26.873030568511187 23.290460105789005 86-87 22.97525333904411 26.862283065457877 26.925969242914043 23.23649435258397 88-89 22.991655489716234 26.720682743251594 26.70477435406338 23.582887412968788 90-91 23.043612663901698 26.47855095610957 26.945724695549135 23.532111684439602 92-93 23.166230891085824 26.639859990245736 26.946052917217127 23.247856201451313 94-95 23.01768779177844 26.542363479001587 27.007338192003736 23.432610537216235 96-97 22.69510204493181 26.604436268551208 27.305346085635513 23.39511560088147 98-99 22.771261849899425 26.81014844824887 26.834612829040598 23.583976872811107 100-101 22.89796640601213 26.502513962755454 26.850558874422443 23.74896075680998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 553.0 1 428.5 2 354.5 3 418.5 4 419.5 5 430.5 6 666.5 7 1271.0 8 2132.5 9 2629.0 10 2463.0 11 2341.0 12 2558.0 13 2785.5 14 3031.5 15 3330.5 16 3681.0 17 4012.5 18 4246.0 19 4679.0 20 5299.5 21 6086.0 22 7452.0 23 9235.5 24 11398.0 25 14641.0 26 19585.5 27 25526.5 28 30509.0 29 36153.0 30 43891.0 31 53224.5 32 62890.5 33 70544.5 34 78387.5 35 86510.5 36 96157.5 37 106090.0 38 112325.5 39 118207.5 40 123864.5 41 128769.5 42 133147.5 43 138742.5 44 146537.5 45 153721.5 46 160160.5 47 173058.5 48 193320.0 49 195215.0 50 178953.5 51 170713.5 52 170480.5 53 169821.5 54 166329.0 55 156503.5 56 143204.5 57 130214.5 58 119276.0 59 106926.5 60 89848.0 61 73340.0 62 58291.0 63 43909.0 64 31246.0 65 22009.5 66 15501.0 67 11183.0 68 8191.0 69 6244.5 70 4523.0 71 2717.5 72 1700.0 73 1115.0 74 692.0 75 409.0 76 251.5 77 137.5 78 94.0 79 64.5 80 51.5 81 46.0 82 41.0 83 33.5 84 22.5 85 22.0 86 23.0 87 17.5 88 13.5 89 14.5 90 10.0 91 6.5 92 5.5 93 2.5 94 3.0 95 5.5 96 4.5 97 1.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05815952634237022 2 0.09505209732475134 3 0.06760653177596539 4 0.12731071066553482 5 0.10297683647994726 6 0.10165604662074794 7 0.11027476010874353 8 0.09733549674235016 9 0.11701302701754009 10-11 0.10795778128794473 12-13 0.09253364208475262 14-15 0.09424619164795175 16-17 0.1095472063727439 18-19 0.08835860295355476 20-21 0.07235242076156295 22-23 0.07485968286716167 24-25 0.06264797323676793 26-27 0.054823972291171925 28-29 0.0677744287919653 30-31 0.04894757673117493 32-33 0.049686323601574565 34-35 0.04500759342237696 36-37 0.049731096139174535 38-39 0.050481036143974145 40-41 0.06455080608476695 42-43 0.06123763830236864 44-45 0.049272177628774776 46-47 0.03651200441278131 48-49 0.04392185938557751 50-51 0.02730005480158602 52-53 0.04143698354877878 54-55 0.04766036627517559 56-57 0.051421259433573674 58-59 0.057241689321570685 60-61 0.054062839151972325 62-63 0.060868264867168835 64-65 0.04175039131197862 66-67 0.06315166428476766 68-69 0.05412999795837228 70-71 0.025397221953587 72-73 0.020416277145589547 74-75 0.02967299929438481 76-77 0.04400021132637747 78-79 0.06265916637116792 80-81 0.04036244264637934 82-83 0.05955866814236951 84-85 0.06391279742396727 86-87 0.06597233415356622 88-89 0.0888734871359545 90-91 0.06355461712316747 92-93 0.07465820644796178 94-95 0.08200090261435801 96-97 0.09041813968315371 98-99 0.12168056406233771 100-101 0.1173824004527399 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4467024.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.840932921535064 #Duplication Level Percentage of deduplicated Percentage of total 1 71.61459345199624 35.69338148443949 2 14.778548988242568 14.731533376012354 3 5.455252377780345 8.156846034929846 4 2.582694165901389 5.148955467181244 5 1.485896254208622 3.7029227767186077 6 0.9414205886116186 2.815276824476624 7 0.596456901422619 2.0809577890073987 8 0.4349265319077638 1.734171528208859 9 0.3163683495688516 1.4191284320422102 >10 1.642832378232121 14.499601571618811 >50 0.10335537121546777 3.504048091604654 >100 0.044726619271106174 3.926125555765176 >500 0.0017568134096416874 0.5542400105250396 >1k 9.009294088481552E-4 0.924354046156322 >5k 1.8018588176957706E-4 0.5859413639839719 >10k+ 9.009294088478853E-5 0.522515647329258 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12113 0.27116487397426114 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11160 0.24983075980787212 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8325 0.18636568775990459 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6898 0.15442048218232093 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5502 0.12316925093753693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5373 0.12028142226233841 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.477253759997708E-5 2.238626879998854E-5 0.0 0.0 0.0 6 4.477253759997708E-5 2.238626879998854E-5 0.0 0.0 0.0 7 4.477253759997708E-5 2.238626879998854E-5 0.0 4.477253759997708E-5 0.0 8 6.715880639996561E-5 2.238626879998854E-5 0.0 8.954507519995416E-5 2.238626879998854E-5 9 6.715880639996561E-5 2.238626879998854E-5 0.0 1.1193134399994269E-4 2.238626879998854E-5 10-11 6.715880639996561E-5 2.238626879998854E-5 0.0 1.3431761279993122E-4 2.238626879998854E-5 12-13 8.954507519995416E-5 2.238626879998854E-5 0.0 1.5670388159991976E-4 2.238626879998854E-5 14-15 1.3431761279993122E-4 2.238626879998854E-5 0.0 1.5670388159991976E-4 2.238626879998854E-5 16-17 1.4551074719992548E-4 2.238626879998854E-5 0.0 1.5670388159991976E-4 2.238626879998854E-5 18-19 2.798283599998567E-4 2.238626879998854E-5 0.0 2.2386268799988538E-4 4.477253759997708E-5 20-21 3.3579403199982807E-4 2.238626879998854E-5 0.0 2.798283599998567E-4 4.477253759997708E-5 22-23 3.3579403199982807E-4 2.238626879998854E-5 0.0 3.5818030079981663E-4 5.5965671999971345E-5 24-25 4.253391071997822E-4 4.477253759997708E-5 0.0 4.4772537599977076E-4 8.954507519995416E-5 26-27 4.253391071997822E-4 5.5965671999971345E-5 0.0 5.596567199997135E-4 8.954507519995416E-5 28-29 4.924979135997479E-4 6.715880639996561E-5 0.0 0.0010297683647994727 8.954507519995416E-5 30-31 4.924979135997479E-4 8.954507519995416E-5 0.0 0.0019028328479990257 8.954507519995416E-5 32-33 4.924979135997479E-4 1.1193134399994269E-4 0.0 0.0030221462879984526 1.1193134399994269E-4 34-35 5.036910479997421E-4 1.3431761279993122E-4 0.0 0.004432481222397731 1.5670388159991976E-4 36-37 5.148841823997363E-4 1.7909015039990832E-4 0.0 0.006077871979196888 1.5670388159991976E-4 38-39 6.044292575996906E-4 1.7909015039990832E-4 0.0 0.007566558854396126 1.5670388159991976E-4 40-41 6.044292575996906E-4 1.7909015039990832E-4 0.0 0.009536550508795118 1.7909015039990832E-4 42-43 6.603949295996619E-4 1.7909015039990832E-4 0.0 0.013017615307193335 1.7909015039990832E-4 44-45 7.163606015996333E-4 1.7909015039990832E-4 0.0 0.018412706087990574 1.7909015039990832E-4 46-47 7.275537359996275E-4 1.7909015039990832E-4 0.0 0.030691574524784285 1.902832847999026E-4 48-49 7.387468703996217E-4 1.7909015039990832E-4 0.0 0.05446579199037212 2.2386268799988538E-4 50-51 7.387468703996217E-4 2.1266955359989113E-4 0.0 0.07200543359516313 2.2386268799988538E-4 52-53 7.387468703996217E-4 2.4624895679987394E-4 0.0 0.0900487662479539 2.3505582239987966E-4 54-55 7.387468703996217E-4 2.4624895679987394E-4 0.0 0.11616234880314052 2.4624895679987394E-4 56-57 7.387468703996217E-4 2.6863522559986245E-4 0.0 0.13622044564793023 2.686352255998625E-4 58-59 7.387468703996217E-4 2.6863522559986245E-4 0.0 0.16358765925591623 2.91021494399851E-4 60-61 7.387468703996217E-4 2.6863522559986245E-4 0.0 0.20624469445429439 3.134077631998395E-4 62-63 7.835194079995988E-4 2.6863522559986245E-4 0.0 0.23566025165747934 3.134077631998395E-4 64-65 7.835194079995988E-4 2.6863522559986245E-4 0.0 0.27207151786066075 3.134077631998395E-4 66-67 8.28291945599576E-4 2.6863522559986245E-4 0.0 0.34240917443022467 3.134077631998395E-4 68-69 8.28291945599576E-4 2.6863522559986245E-4 0.0 0.4201119134349849 3.134077631998395E-4 70-71 8.394850799995702E-4 2.6863522559986245E-4 0.0 0.48540146639015147 3.134077631998395E-4 72-73 8.618713487995586E-4 2.6863522559986245E-4 0.0 0.5592201877581137 4.029528383997937E-4 74-75 8.842576175995473E-4 2.6863522559986245E-4 0.0 0.6255618953468797 4.029528383997937E-4 76-77 9.178370207995301E-4 2.6863522559986245E-4 0.0 0.6802179706220517 4.14145972799788E-4 78-79 9.178370207995301E-4 2.6863522559986245E-4 0.0 0.7496937558428162 4.253391071997822E-4 80-81 0.0010297683647994727 2.6863522559986245E-4 0.0 0.8459994842203669 4.253391071997822E-4 82-83 0.0010745409023994498 2.6863522559986245E-4 0.0 0.9688665205291039 4.5891851039976504E-4 84-85 0.0010745409023994498 2.6863522559986245E-4 0.0 1.1124296623434304 4.701116447997593E-4 86-87 0.0010745409023994498 2.6863522559986245E-4 0.0 1.3500151331177088 4.701116447997593E-4 88-89 0.0010745409023994498 2.6863522559986245E-4 0.0 1.634611768371963 4.701116447997593E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16560 0.0 23.623398 1 GTATCAA 28235 0.0 23.417591 1 TACATGG 16590 0.0 22.9795 2 GAGTACT 12160 0.0 21.645847 12-13 ACATGGG 16895 0.0 21.492378 3 AGAGTAC 20220 0.0 21.279392 10-11 GTACTTT 12445 0.0 20.616352 14-15 TATCAAC 32050 0.0 20.467447 2 TCAACGC 31930 0.0 20.437407 4 CAACGCA 31870 0.0 20.428396 5 ATCAACG 32080 0.0 20.371248 3 AACGCAG 32550 0.0 20.118198 6 AGTACTT 12805 0.0 19.460962 12-13 TACCGTA 310 5.5642886E-9 18.39483 7 ACGCAGA 35515 0.0 18.15701 7 CGCAGAG 35800 0.0 17.920952 8 GGTATCA 21740 0.0 17.885315 1 CATGGGG 10130 0.0 17.875975 4 CATGGGA 7495 0.0 17.311918 4 GCAGAGT 36785 0.0 16.79549 9 >>END_MODULE