FastQCFastQC Report
Thu 26 May 2016
SRR937115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4467024
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133940.2998416843070465No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT125370.2806566519454563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98330.22012418111028728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63080.14121258359032768No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59820.13391465996153143No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53070.11880392852153918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG163950.022.4560321
GTATCAA303950.022.2718071
TACATGG162950.021.5999682
AGAGTAC224900.021.0034210-11
GAGTACT144150.020.93971412-13
ACATGGG167350.020.1801033
GTACTTT150850.019.75756314-15
AGTACTT151600.019.75402812-13
TCAACGC343550.019.4803034
CAACGCA345450.019.3731615
ATCAACG346300.019.3395393
TATCAAC347550.019.3250852
AACGCAG353150.018.9507526
GGTATCA237750.017.3636881
TACTTTT169400.017.31364414-15
GAGTACA132200.017.2844811
ACGCAGA385500.017.249577
CGCAGAG386950.016.976268
ACTTTTT179650.016.74882516-17
AGTACAT126250.016.7047822
Produced by FastQC (version 0.11.5)