##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937115_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4467024 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03074216749227 31.0 31.0 34.0 30.0 34.0 2 32.0302044493157 33.0 31.0 34.0 30.0 34.0 3 32.21856587293912 33.0 31.0 34.0 30.0 34.0 4 35.96677429984706 37.0 35.0 37.0 35.0 37.0 5 35.879080121351485 37.0 35.0 37.0 35.0 37.0 6 35.908289277156335 37.0 35.0 37.0 35.0 37.0 7 35.84354169576881 37.0 35.0 37.0 35.0 37.0 8 35.818701220320285 37.0 35.0 37.0 35.0 37.0 9 37.483563105996296 39.0 37.0 39.0 35.0 39.0 10-11 37.4054440943232 39.0 37.0 39.0 34.0 39.0 12-13 37.312318335428685 39.0 37.0 39.0 34.0 39.0 14-15 38.50466339558507 40.0 38.0 41.0 34.0 41.0 16-17 38.419301978229804 40.0 38.0 41.0 34.0 41.0 18-19 38.46120067857258 40.0 38.0 41.0 33.5 41.0 20-21 38.531347492200624 40.0 38.0 41.0 34.0 41.0 22-23 38.45766006629917 40.0 38.0 41.0 34.0 41.0 24-25 38.358416475935655 40.0 38.0 41.0 34.0 41.0 26-27 38.18881183535168 40.0 38.0 41.0 33.5 41.0 28-29 38.02051052333724 40.0 37.0 41.0 33.0 41.0 30-31 37.91158364047294 40.0 37.0 41.0 33.0 41.0 32-33 37.92949209585621 40.0 37.0 41.0 33.0 41.0 34-35 37.85510207690847 40.0 37.0 41.0 33.0 41.0 36-37 37.708109022919956 40.0 37.0 41.0 33.0 41.0 38-39 37.556962196755606 40.0 37.0 41.0 32.5 41.0 40-41 37.3959980962717 40.0 37.0 41.0 32.0 41.0 42-43 37.193294349884845 40.0 36.0 41.0 31.0 41.0 44-45 36.89061677304622 39.0 36.0 41.0 31.0 41.0 46-47 36.662939464842815 39.0 36.0 41.0 30.5 41.0 48-49 36.368913733170004 39.0 35.5 41.0 30.0 41.0 50-51 36.11862786051743 39.0 35.0 40.0 29.5 41.0 52-53 35.8659775501542 39.0 35.0 40.0 28.5 41.0 54-55 35.62517304585782 38.0 35.0 40.0 28.0 41.0 56-57 35.329406222129094 38.0 34.0 40.0 28.0 41.0 58-59 34.99032957960378 38.0 34.0 40.0 27.0 41.0 60-61 34.6336634860256 37.5 34.0 40.0 26.0 41.0 62-63 34.255458331990155 37.0 33.0 39.5 26.0 41.0 64-65 33.841804745172624 36.0 33.0 39.0 26.0 40.5 66-67 33.43735795912446 36.0 32.5 39.0 25.0 40.0 68-69 32.98870008309783 35.0 32.0 38.5 24.5 40.0 70-71 32.498990267345775 35.0 31.5 38.0 23.0 40.0 72-73 32.14618614092962 35.0 31.0 37.0 22.5 39.0 74-75 31.701895042426457 35.0 31.0 36.5 21.5 39.0 76-77 30.301862828585655 33.5 29.5 35.0 20.0 37.5 78-79 31.04481898910774 34.0 31.0 36.0 20.0 37.5 80-81 30.890326423139882 34.0 31.0 35.0 20.0 37.0 82-83 30.56515422796027 34.0 30.5 35.0 19.0 37.0 84-85 30.189790898817648 34.0 30.0 35.0 18.0 36.0 86-87 29.847843553112767 34.0 30.0 35.0 15.0 36.0 88-89 29.492649468639524 34.0 29.5 35.0 8.5 35.5 90-91 29.064063568944334 34.0 29.0 35.0 4.5 35.0 92-93 28.596489071919024 33.5 29.0 35.0 2.0 35.0 94-95 28.105665203500138 33.0 28.5 35.0 2.0 35.0 96-97 27.4564647514766 33.0 27.0 35.0 2.0 35.0 98-99 26.69879432033497 33.0 25.5 34.5 2.0 35.0 100-101 23.67027690023604 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3778097496800541 1101 2 0.27265901136519943 1101 3 0.24597699115889782 1101 4 0.23763952102954278 1101 5 0.26789870535882443 1101 6 0.22513187834746873 1101 7 0.3039287537686448 1101 8 0.32238143958294785 1101 9 0.4099804191990657 1101 10-11 0.34217148534126096 1101 12-13 0.37360722005113445 1101 14-15 0.49345910499960866 1101 16-17 0.5303904051057131 1101 18-19 0.5170424111914613 1101 20-21 0.4848214262606163 1101 22-23 0.4154548745268585 1101 24-25 0.46767064386389734 1101 26-27 0.3869744119160572 1101 28-29 0.4580589078132178 1101 30-31 0.4814771072724966 1101 32-33 0.5627090853753174 1101 34-35 0.6209380107202236 1101 36-37 0.5516632599621403 1101 38-39 0.5002290944567065 1101 40-41 0.4833959449620906 1101 42-43 0.5709893809733373 1101 44-45 0.6031320509515083 1101 46-47 0.6158980773799883 1101 48-49 0.512679984407292 1101 50-51 0.6206517916087009 1101 52-53 0.5043555450961605 1101 54-55 0.5838470465108756 1101 56-57 0.6664809697021141 1101 58-59 0.5802700332514945 1101 60-61 0.6423577618538232 1101 62-63 0.6531930684367211 1101 64-65 0.6377008869020884 1101 66-67 0.5985753804058547 1101 68-69 0.4983806607855783 1101 70-71 0.5614810706056304 1101 72-73 0.6003242950107861 1101 74-75 0.536669783300173 1101 76-77 0.49770198281061795 1101 78-79 0.6165426075987455 1101 80-81 0.7333107866534228 1101 82-83 0.8383746413461814 1101 84-85 0.8043978852894895 1101 86-87 0.6427754429010442 1101 88-89 0.6419204928774391 1101 90-91 0.7679057002722587 1101 92-93 0.7632636693020878 1101 94-95 0.7367625262235329 1101 96-97 0.8330371395267875 1101 98-99 0.8909770432896309 1101 100-101 0.8907416336146312 1102 1 0.24980018544827232 1102 2 0.1147883869275006 1102 3 0.22443805413846718 1102 4 0.17895602054008464 1102 5 0.2214067827188586 1102 6 0.20443929923420257 1102 7 0.20098140393124453 1102 8 0.24829214715074954 1102 9 0.3344603662972929 1102 10-11 0.32973532044675125 1102 12-13 0.2696323668837053 1102 14-15 0.4111031842915125 1102 16-17 0.37664493896938467 1102 18-19 0.3408782122982288 1102 20-21 0.29156114104628017 1102 22-23 0.3230932942625273 1102 24-25 0.3609948770006355 1102 26-27 0.3141059194426461 1102 28-29 0.3183935854837756 1102 30-31 0.30950390772569847 1102 32-33 0.41656006008033586 1102 34-35 0.3684619713793751 1102 36-37 0.4781987640467804 1102 38-39 0.5686268016650331 1102 40-41 0.5689990112924903 1102 42-43 0.5462920769781192 1102 44-45 0.6579815377941998 1102 46-47 0.5684862292057815 1102 48-49 0.5960614272705413 1102 50-51 0.6043689555612701 1102 52-53 0.5958510437567739 1102 54-55 0.5939360695032931 1102 56-57 0.6601883400468793 1102 58-59 0.5944769062112982 1102 60-61 0.5941448146118447 1102 62-63 0.7604652349769339 1102 64-65 0.6181329547269527 1102 66-67 0.7015657657641796 1102 68-69 0.4060698426241913 1102 70-71 0.4591344350037829 1102 72-73 0.48090347607674033 1102 74-75 0.3482123722862944 1102 76-77 0.468062417955867 1102 78-79 0.5984199865219928 1102 80-81 0.5735386966085834 1102 82-83 0.6516957090057254 1102 84-85 0.496267165610103 1102 86-87 0.34254212653977234 1102 88-89 0.6120790683158432 1102 90-91 0.7112142931339989 1102 92-93 0.6785545310889454 1102 94-95 0.7888945895797441 1102 96-97 0.8740875672514363 1102 98-99 1.0025852091641596 1102 100-101 1.2646045658106821 1103 1 -0.018847652988583263 1103 2 -0.16860141063258283 1103 3 -0.06420822401850756 1103 4 0.10138964789665295 1103 5 0.13056652084762277 1103 6 0.12356628427470184 1103 7 0.15114836666741382 1103 8 0.16818597138394153 1103 9 0.13857464528812358 1103 10-11 0.1804351443397465 1103 12-13 0.26089384037621954 1103 14-15 0.24613735058409247 1103 16-17 0.3069154556906071 1103 18-19 0.2983513774027031 1103 20-21 0.2502766470309723 1103 22-23 0.30939224644506425 1103 24-25 0.3184765469093591 1103 26-27 0.33066042500658455 1103 28-29 0.3169164992169158 1103 30-31 0.35522177075614536 1103 32-33 0.3325061843834831 1103 34-35 0.3290527547293962 1103 36-37 0.36213913577706336 1103 38-39 0.3744641667871562 1103 40-41 0.3851024425014131 1103 42-43 0.40627927500960936 1103 44-45 0.5271161963422983 1103 46-47 0.5385797564742276 1103 48-49 0.46624889106117706 1103 50-51 0.33184362978889936 1103 52-53 0.435492511903405 1103 54-55 0.367485616702389 1103 56-57 0.43545553797604697 1103 58-59 0.49435508927816585 1103 60-61 0.4447721238580371 1103 62-63 0.42159009445582285 1103 64-65 0.23500795712492106 1103 66-67 0.3954797150499729 1103 68-69 0.4065529538734296 1103 70-71 0.3403886314532514 1103 72-73 0.41849317618326864 1103 74-75 0.4410510713198157 1103 76-77 0.3303292872031349 1103 78-79 0.37542082165540336 1103 80-81 0.4351887437367097 1103 82-83 0.47717891617380204 1103 84-85 0.3673308800226138 1103 86-87 0.15769404280645816 1103 88-89 0.43468669245720903 1103 90-91 0.7039237575103776 1103 92-93 0.6205490803407123 1103 94-95 0.6504013339534644 1103 96-97 0.9181695024077392 1103 98-99 0.850171507348989 1103 100-101 0.7548625363469732 1104 1 0.20398003356375938 1104 2 0.06599273659172411 1104 3 0.11281493231227557 1104 4 0.13753469675287988 1104 5 0.13823377832604677 1104 6 0.144286659136867 1104 7 0.20688327084538827 1104 8 0.21645811706100915 1104 9 0.24242647202655832 1104 10-11 0.20316024901979546 1104 12-13 0.2259063172236253 1104 14-15 0.23021713426888368 1104 16-17 0.22482461360519324 1104 18-19 0.2869213719765398 1104 20-21 0.22847889244802388 1104 22-23 0.22172317747630643 1104 24-25 0.18079596916027896 1104 26-27 0.21911096924309703 1104 28-29 0.2928010757062651 1104 30-31 0.31106818961426086 1104 32-33 0.3717875628445313 1104 34-35 0.35918415425525296 1104 36-37 0.23157302011922098 1104 38-39 0.17013416476792287 1104 40-41 0.3789759905046637 1104 42-43 0.3215663006671221 1104 44-45 0.37301358621738956 1104 46-47 0.39452767695811275 1104 48-49 0.38876106253120213 1104 50-51 0.3480890418572429 1104 52-53 0.34879994424881033 1104 54-55 0.4093220274918963 1104 56-57 0.36862396752196247 1104 58-59 0.2738679645703499 1104 60-61 0.3568821029261855 1104 62-63 0.45710282516115797 1104 64-65 0.27675333637039756 1104 66-67 0.43351951631785113 1104 68-69 0.39853794010859644 1104 70-71 0.37143105273444377 1104 72-73 0.48790562178663066 1104 74-75 0.31515070661196987 1104 76-77 0.284407342493477 1104 78-79 0.36645020519194915 1104 80-81 0.48536444998942585 1104 82-83 0.5364868603898714 1104 84-85 0.6997057438492291 1104 86-87 0.5522372605617214 1104 88-89 0.6330697711431981 1104 90-91 0.7688874570722106 1104 92-93 0.7666375907555363 1104 94-95 0.759702630498591 1104 96-97 0.8524360025502489 1104 98-99 0.8797171224205904 1104 100-101 0.8965766299910491 1105 1 0.13562292188076697 1105 2 -0.027420058568328187 1105 3 0.04560723294284941 1105 4 0.09633644852496559 1105 5 0.10930282583759521 1105 6 0.05120871731857335 1105 7 0.10662291296618065 1105 8 0.17310777769278474 1105 9 0.20452408365687802 1105 10-11 0.17390290858305235 1105 12-13 0.2282079636485932 1105 14-15 0.25335211402718016 1105 16-17 0.32549695396668454 1105 18-19 0.24659411867691716 1105 20-21 0.1646441820773603 1105 22-23 0.2671748156240028 1105 24-25 0.23779910699946072 1105 26-27 0.2684961935572048 1105 28-29 0.2583271716925424 1105 30-31 0.30504271066111244 1105 32-33 0.2604685792937289 1105 34-35 0.2374913013047788 1105 36-37 0.08854173202495019 1105 38-39 0.17555013539112707 1105 40-41 0.05819119209389356 1105 42-43 0.13917187352971183 1105 44-45 0.15489990162667056 1105 46-47 0.1618883177695878 1105 48-49 0.11674445531040334 1105 50-51 0.15385999464668743 1105 52-53 0.19023748093218273 1105 54-55 0.14687789908880688 1105 56-57 0.21543044486480056 1105 58-59 0.19118646653432592 1105 60-61 0.21091050149948387 1105 62-63 0.06190627753674249 1105 64-65 0.12617844209375306 1105 66-67 0.13516424820942774 1105 68-69 -0.0016287340828711194 1105 70-71 -0.050232077278955956 1105 72-73 0.03674717972001673 1105 74-75 0.0805050491707 1105 76-77 0.08549881389173208 1105 78-79 0.05817819893131926 1105 80-81 0.23260002872134677 1105 82-83 0.281899557727602 1105 84-85 0.269951578875645 1105 86-87 0.3118962745126943 1105 88-89 0.362357775116287 1105 90-91 0.5108669670576163 1105 92-93 0.5660547954449875 1105 94-95 0.49997307160202453 1105 96-97 0.7228938486531575 1105 98-99 0.7037093643577741 1105 100-101 0.7846236825291619 1106 1 0.315139238150941 1106 2 0.20027326999977646 1106 3 0.18879726055074997 1106 4 0.17676583128307755 1106 5 0.17792931353354646 1106 6 0.26932918705733044 1106 7 0.2623182337265817 1106 8 0.2722989505885778 1106 9 0.35858905777656247 1106 10-11 0.31366368314059656 1106 12-13 0.37936103122763853 1106 14-15 0.5417878237030109 1106 16-17 0.443447272743434 1106 18-19 0.4373697728845869 1106 20-21 0.38010559652646947 1106 22-23 0.4370674291108685 1106 24-25 0.4670185196293204 1106 26-27 0.4580881021687233 1106 28-29 0.47724692601604346 1106 30-31 0.45774023292760546 1106 32-33 0.43286439928980514 1106 34-35 0.45800658622205503 1106 36-37 0.4835861722385886 1106 38-39 0.6642772050462256 1106 40-41 0.5721312264748022 1106 42-43 0.6081255392860072 1106 44-45 0.6592259056370295 1106 46-47 0.42353963197445665 1106 48-49 0.36760645980916706 1106 50-51 0.3161148117401993 1106 52-53 0.36783327817837375 1106 54-55 0.5564205206104944 1106 56-57 0.5693371161482546 1106 58-59 0.49317345846126415 1106 60-61 0.47408237930030594 1106 62-63 0.49908629927716675 1106 64-65 0.6030767308050216 1106 66-67 0.5047014689527245 1106 68-69 0.6538153822881796 1106 70-71 0.6194619386103355 1106 72-73 0.6341853031847293 1106 74-75 0.6159765934426069 1106 76-77 0.5441697770580731 1106 78-79 0.5702778538233417 1106 80-81 0.5576347334311684 1106 82-83 0.7206001430639795 1106 84-85 0.6274610731490178 1106 86-87 0.46868601602128734 1106 88-89 0.7756294944843845 1106 90-91 0.9864846700688013 1106 92-93 1.0639925999288309 1106 94-95 1.0166073948337413 1106 96-97 1.1871737210533908 1106 98-99 1.1961119740628803 1106 100-101 1.2169632531538284 1107 1 0.19346916954568627 1107 2 0.10413032575041825 1107 3 0.115343583099488 1107 4 0.11931804512419575 1107 5 0.1632369160617131 1107 6 0.16856756718825494 1107 7 0.16741750061978422 1107 8 0.1697049239809303 1107 9 0.24675383719750243 1107 10-11 0.19181701698436626 1107 12-13 0.21907426831526067 1107 14-15 0.22687838872967347 1107 16-17 0.23027351814163666 1107 18-19 0.29444207129270694 1107 20-21 0.18791046855340454 1107 22-23 0.2907299033249302 1107 24-25 0.27695579567610196 1107 26-27 0.268253372306539 1107 28-29 0.2827879725898583 1107 30-31 0.30195609512954036 1107 32-33 0.26882371955279183 1107 34-35 0.348720970269099 1107 36-37 0.2821414203008601 1107 38-39 0.2712931113627306 1107 40-41 0.3144232825583231 1107 42-43 0.3162552278393065 1107 44-45 0.33544362656842 1107 46-47 0.24267676860692688 1107 48-49 0.29156900143436104 1107 50-51 0.286171523739263 1107 52-53 0.3019894589101213 1107 54-55 0.2937665483448413 1107 56-57 0.2605450222618515 1107 58-59 0.24546142107455893 1107 60-61 0.268440259268921 1107 62-63 0.031095247847105156 1107 64-65 0.10323879802574254 1107 66-67 0.041781983121786936 1107 68-69 0.17766455371351242 1107 70-71 0.23780191970985953 1107 72-73 0.2704782398302754 1107 74-75 0.3002678462030097 1107 76-77 0.30168817822027094 1107 78-79 0.43290643043411237 1107 80-81 0.42525775681390243 1107 82-83 0.5685968212143315 1107 84-85 0.5476057644964634 1107 86-87 0.6212684615954736 1107 88-89 0.49336596492162244 1107 90-91 0.7494041510963214 1107 92-93 0.8502386408341351 1107 94-95 0.7664115836229364 1107 96-97 0.9369644842977749 1107 98-99 0.9353309253409776 1107 100-101 0.7796284365899986 1108 1 -0.002729904629951818 1108 2 -0.11530169130121948 1108 3 -0.06366799898684405 1108 4 0.01692163501404309 1108 5 0.008216521261587673 1108 6 0.016362869886911824 1108 7 0.008944165745099042 1108 8 0.08598566173772326 1108 9 0.07865520298250317 1108 10-11 0.049334741137315063 1108 12-13 -5.334714143501174E-4 1108 14-15 -0.01636875540764038 1108 16-17 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-1.1468017974942484 2316 50-51 -1.1778923059497046 2316 52-53 -0.8976240015306516 2316 54-55 -0.984961998137095 2316 56-57 -1.1051124423557965 2316 58-59 -0.9818207798424865 2316 60-61 -0.5959146754780491 2316 62-63 -0.6619730877975201 2316 64-65 -0.5913762347075817 2316 66-67 -0.6630916811512222 2316 68-69 -0.7974878836093637 2316 70-71 -0.6461668353539025 2316 72-73 -0.5638952699077393 2316 74-75 -0.6357225749366044 2316 76-77 -0.9042655952241141 2316 78-79 -0.630035788873176 2316 80-81 -0.7797262277410653 2316 82-83 -0.5675019861142054 2316 84-85 -0.658397612179801 2316 86-87 -0.7216232365074227 2316 88-89 -0.6347483578744644 2316 90-91 -0.6092488656846768 2316 92-93 -0.9826335412115519 2316 94-95 -0.6211561721786687 2316 96-97 -0.6329076577189241 2316 98-99 -0.46613247103416455 2316 100-101 -0.4053648200647224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 13.0 4 29.0 5 66.0 6 160.0 7 505.0 8 1246.0 9 2386.0 10 3933.0 11 6168.0 12 9418.0 13 12338.0 14 15737.0 15 20964.0 16 26979.0 17 35788.0 18 32987.0 19 22929.0 20 21546.0 21 22616.0 22 24497.0 23 27232.0 24 32576.0 25 39175.0 26 47963.0 27 60604.0 28 75989.0 29 94608.0 30 118138.0 31 147222.0 32 185098.0 33 238969.0 34 319160.0 35 447628.0 36 677767.0 37 975547.0 38 670161.0 39 48880.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.62683567566769 19.228054007470664 15.116010649600081 23.029099667261573 2 15.560353801621254 20.886295000346998 39.29226243644936 24.261088761582393 3 19.762878854869637 24.43393259106117 29.545536460846023 26.25765209322318 4 13.49694281577938 17.18824119619818 36.68820302756715 32.62661296045528 5 14.225334171789674 37.14810074639479 33.82217666281093 14.804388419004603 6 32.031872795363874 36.10397297340255 17.267982790266505 14.596171440967073 7 28.49324930720079 32.48720680417361 20.755142742717016 18.264401145908586 8 26.382799823775294 34.29686072875364 19.700050861602712 19.620288585868355 9 26.146288893903414 17.164895465079212 20.3084872613176 36.38032837969977 10-11 25.016028582813597 26.450777028085838 27.202329517217294 21.330864871883268 12-13 26.16814385434668 23.750543011004837 27.614900904759033 22.46641222988945 14-15 23.08946627553378 25.174288295742308 26.082364903345045 25.653880525378863 16-17 22.391316903602938 28.223779411079946 26.52224389213042 22.862659793186694 18-19 22.299499622119782 27.256200101006844 28.636156868644534 21.808143408228833 20-21 23.307673640586998 26.040558522546142 28.583447058022877 22.068320778843983 22-23 22.907208468098673 25.9740713280251 28.567923521342177 22.550796682534056 24-25 22.95500660250312 26.163732450925046 28.538428448667542 22.34283249790429 26-27 22.71578176560984 26.142337181112076 28.786266780047548 22.355614273230533 28-29 22.510219615393225 26.69171013454521 28.53485638809913 22.263213861962434 30-31 22.77495778973838 26.13612828964087 28.42120299637443 22.66771092424632 32-33 22.680893678984006 26.22404701325533 28.491421135302243 22.603638172458425 34-35 23.110111418804415 26.246471290125434 28.328147071926356 22.315270219143795 36-37 23.10241429804961 26.106479581682564 28.476618966353673 22.314487153914158 38-39 22.787973463606484 26.473654565716615 28.358196091178666 22.380175879498232 40-41 22.903671817667725 26.363560857645485 28.21433480626323 22.518432518423566 42-43 22.750181500615525 26.40617364017814 27.93386818275741 22.90977667644893 44-45 22.999187149666767 26.260617591639306 27.901171637231904 22.839023621462022 46-47 23.380756847270018 26.632658143828632 27.545134981002423 22.44145002789893 48-49 23.239433939666647 26.636892869175497 27.444423177446236 22.679250013711616 50-51 22.95855982671214 26.81212612082602 27.553907326990846 22.675406725471 52-53 23.29153397993049 26.94514744318023 27.00041974714432 22.762898829744962 54-55 23.07855301930035 26.711115961617573 27.152373996434875 23.0579570226472 56-57 23.22963336400638 26.863779325993665 27.067987715707986 22.83859959429197 58-59 23.499465857886857 26.63395773785218 27.077871025051397 22.78870537920956 60-61 23.2453641508661 26.741528562579514 27.486304508570775 22.526802777983608 62-63 22.902238716425074 26.771873175519094 27.57898771083388 22.746900397221953 64-65 22.990612634008688 26.937387375841293 27.187979801722157 22.884020188427865 66-67 23.096575648897144 26.834357076633726 26.963346829649243 23.105720444819887 68-69 22.96340057311093 26.75811980841381 27.27463824625055 23.003841372224702 70-71 23.32231404588656 26.607650386745874 27.038844275462637 23.031191291904932 72-73 23.19629014628872 26.609089163978915 27.16841027776332 23.026210411969046 74-75 23.010613724044518 27.227941261242584 26.758655334526004 23.00278968018689 76-77 23.20016807725375 26.82923089097846 26.77675711863024 23.19384391313755 78-79 23.088191146970022 26.963107114306585 26.876337722769104 23.072364015954285 80-81 23.272727720477558 26.854293438297937 26.781366110743644 23.09161273048086 82-83 23.24697746243403 26.81032124437703 27.00157064945101 22.94113064373793 84-85 23.056429458755115 26.991948946790245 26.80122251178622 23.150399082668418 86-87 23.078390268757172 26.940291457313432 26.83951759283553 23.14180068109387 88-89 23.11599704632098 26.846634235297895 26.59218891367451 23.445179804706612 90-91 23.079136738963268 26.648074832970114 26.857756242143804 23.415032185922815 92-93 23.17750465019081 26.768230997794728 26.864066678889575 23.190197673124885 94-95 23.074690472867175 26.691792316368723 26.88139318074898 23.352124030015116 96-97 22.79049109252099 26.74163357826937 27.141604609947933 23.326270719261707 98-99 22.75835992144902 26.908176752241147 26.662223623141557 23.671239703168276 100-101 22.653082400274098 26.82141172959861 26.664930221895926 23.86057564823136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 572.0 1 416.5 2 308.5 3 365.5 4 352.5 5 361.0 6 580.5 7 1238.5 8 2158.0 9 2688.5 10 2567.5 11 2355.5 12 2528.0 13 2876.5 14 3240.0 15 3599.5 16 3975.5 17 4400.0 18 4777.5 19 5335.5 20 5955.0 21 6815.0 22 8278.5 23 10269.0 24 12570.5 25 15736.5 26 20732.5 27 26539.5 28 31534.5 29 37682.5 30 45293.5 31 54175.0 32 63542.0 33 71216.5 34 78569.0 35 86179.0 36 96023.5 37 105921.5 38 112836.0 39 118148.0 40 123250.5 41 128209.0 42 132562.0 43 138789.0 44 145888.0 45 152422.0 46 159149.5 47 173011.0 48 194290.0 49 195119.5 50 177596.0 51 169061.0 52 168913.0 53 168527.0 54 166169.0 55 157415.0 56 143396.0 57 129925.0 58 119295.0 59 106874.0 60 89001.0 61 72498.5 62 57687.0 63 43155.5 64 30519.0 65 21214.0 66 14865.0 67 10877.5 68 8086.5 69 6081.0 70 4383.5 71 2544.0 72 1482.5 73 977.0 74 575.0 75 337.5 76 206.5 77 94.5 78 46.5 79 32.5 80 22.5 81 13.5 82 9.0 83 10.0 84 5.5 85 2.0 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01685686040639137 2 0.0025520346431986934 3 0.0024401032991987507 4 1.1193134399994269E-4 5 0.0019028328479990255 6 3.8056656959980514E-4 7 2.238626879998854E-5 8 0.0 9 0.0 10-11 8.954507519995416E-5 12-13 7.49940004799616E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0021490818047988996 22-23 0.0 24-25 0.003525837335998195 26-27 0.0016677770255991461 28-29 0.002775897331198579 30-31 0.015513684278392056 32-33 6.380086607996734E-4 34-35 0.0021043092671989227 36-37 0.0039623695775979715 38-39 0.0022833994175988307 40-41 0.004253391071997822 42-43 0.002171468073598888 44-45 6.044292575996905E-4 46-47 1.455107471999255E-4 48-49 2.0147641919989688E-4 50-51 1.3431761279993122E-4 52-53 0.0010969271711994382 54-55 0.0030557256911984353 56-57 4.14145972799788E-4 58-59 0.0010745409023994498 60-61 9.626095583995072E-4 62-63 0.0 64-65 2.126695535998911E-4 66-67 5.5965671999971345E-5 68-69 1.119313439999427E-5 70-71 1.7909015039990832E-4 72-73 3.3579403199982806E-5 74-75 5.484635855997191E-4 76-77 6.827811983996504E-4 78-79 2.4624895679987394E-4 80-81 0.005562987796797152 82-83 0.0018356740415990602 84-85 0.0034698716639982234 86-87 0.0020595367295989453 88-89 0.0011752791119993982 90-91 2.0147641919989688E-4 92-93 6.715880639996561E-5 94-95 1.9028328479990257E-4 96-97 0.0016901632943991346 98-99 0.0025968071807986703 100-101 9.514164239995128E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4467024.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.78323997197892 #Duplication Level Percentage of deduplicated Percentage of total 1 69.44899428473785 33.184979597202215 2 15.759230880850916 15.060542219070406 3 5.980501395850592 8.573032000520513 4 2.844786006540246 5.437323696777607 5 1.5650679843787443 3.739200953501545 6 0.9872238158687877 2.8303651499826614 7 0.6889170671511384 2.3043082678332336 8 0.4635225864242076 1.7718888783632203 9 0.35008889572334356 1.5055543544286267 >10 1.748739095442558 14.951032250517498 >50 0.11041583786390559 3.6284784929950566 >100 0.049173511141658424 4.155241531612468 >500 0.0019749692406349174 0.6237210916164592 >1k 0.0010815304161486943 1.0353556104822301 >5k 1.8809224628665473E-4 0.6163285747416781 >10k+ 9.404612314332736E-5 0.5826473303545919 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13394 0.2998416843070465 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12537 0.2806566519454563 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9833 0.22012418111028728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6308 0.14121258359032768 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5982 0.13391465996153143 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5307 0.11880392852153918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.238626879998854E-5 2 0.0 0.0 0.0 2.238626879998854E-5 2.238626879998854E-5 3 0.0 0.0 0.0 2.238626879998854E-5 2.238626879998854E-5 4 0.0 0.0 0.0 4.477253759997708E-5 2.238626879998854E-5 5 0.0 0.0 0.0 4.477253759997708E-5 2.238626879998854E-5 6 0.0 0.0 0.0 4.477253759997708E-5 4.477253759997708E-5 7 2.238626879998854E-5 0.0 0.0 4.477253759997708E-5 4.477253759997708E-5 8 2.238626879998854E-5 0.0 0.0 6.715880639996561E-5 4.477253759997708E-5 9 2.238626879998854E-5 0.0 0.0 8.954507519995416E-5 4.477253759997708E-5 10-11 2.238626879998854E-5 0.0 0.0 8.954507519995416E-5 4.477253759997708E-5 12-13 1.0073820959994842E-4 0.0 0.0 1.1193134399994269E-4 4.477253759997708E-5 14-15 1.1193134399994269E-4 0.0 0.0 1.1193134399994269E-4 5.5965671999971345E-5 16-17 1.3431761279993122E-4 0.0 0.0 1.1193134399994269E-4 6.715880639996561E-5 18-19 1.6789701599991404E-4 0.0 0.0 2.5744209119986817E-4 6.715880639996561E-5 20-21 1.902832847999026E-4 0.0 0.0 3.022146287998453E-4 6.715880639996561E-5 22-23 2.0147641919989685E-4 0.0 0.0 4.0295283839979364E-4 6.715880639996561E-5 24-25 2.2386268799988538E-4 0.0 0.0 4.924979135997479E-4 1.0073820959994842E-4 26-27 2.2386268799988538E-4 0.0 0.0 6.156223919996848E-4 1.1193134399994269E-4 28-29 2.4624895679987394E-4 0.0 0.0 0.0010633477679994556 1.1193134399994269E-4 30-31 2.4624895679987394E-4 0.0 0.0 0.0019699916543989915 1.1193134399994269E-4 32-33 2.5744209119986817E-4 0.0 0.0 0.0032012364383983607 1.1193134399994269E-4 34-35 2.9102149439985095E-4 0.0 0.0 0.004701116447997594 1.3431761279993122E-4 36-37 3.3579403199982807E-4 2.238626879998854E-5 0.0 0.006402472876796721 1.3431761279993122E-4 38-39 3.5818030079981663E-4 2.238626879998854E-5 0.0 0.007924739155195943 1.3431761279993122E-4 40-41 3.5818030079981663E-4 2.238626879998854E-5 0.0 0.009928310212794916 1.3431761279993122E-4 42-43 4.2533910719978226E-4 2.238626879998854E-5 0.0 0.013420568145593129 1.4551074719992548E-4 44-45 5.148841823997363E-4 2.238626879998854E-5 0.0 0.01877088638879039 1.5670388159991976E-4 46-47 6.492017951996676E-4 2.238626879998854E-5 0.0 0.031105720497584075 1.5670388159991976E-4 48-49 6.939743327996447E-4 2.238626879998854E-5 0.0 0.055059028113571815 1.5670388159991976E-4 50-51 6.939743327996447E-4 4.477253759997708E-5 0.0 0.07271060106236277 1.5670388159991976E-4 52-53 6.939743327996447E-4 6.715880639996561E-5 0.0 0.09084347879035348 2.0147641919989685E-4 54-55 6.939743327996447E-4 6.715880639996561E-5 0.0 0.11723688970553997 2.4624895679987394E-4 56-57 7.387468703996217E-4 6.715880639996561E-5 0.0 0.13725021401272972 2.4624895679987394E-4 58-59 7.387468703996217E-4 1.1193134399994269E-4 0.0 0.16466220015831567 2.4624895679987394E-4 60-61 7.387468703996217E-4 1.3431761279993122E-4 0.0 0.2072184971470939 2.4624895679987394E-4 62-63 7.387468703996217E-4 1.5670388159991976E-4 0.0 0.2366564406190788 2.4624895679987394E-4 64-65 7.387468703996217E-4 1.5670388159991976E-4 0.0 0.2730229342846602 2.4624895679987394E-4 66-67 8.059056767995874E-4 1.5670388159991976E-4 0.0 0.3431591144350243 2.4624895679987394E-4 68-69 8.28291945599576E-4 1.5670388159991976E-4 0.0 0.42107452299338444 2.4624895679987394E-4 70-71 8.28291945599576E-4 1.5670388159991976E-4 0.0 0.48675583565255076 2.4624895679987394E-4 72-73 8.28291945599576E-4 1.5670388159991976E-4 0.0 0.5609551235901128 2.91021494399851E-4 74-75 8.394850799995702E-4 1.5670388159991976E-4 0.0 0.6276885908828786 2.91021494399851E-4 76-77 8.506782143995644E-4 1.5670388159991976E-4 0.0 0.6826468807868504 2.91021494399851E-4 78-79 8.618713487995586E-4 1.5670388159991976E-4 0.0 0.7524808463084147 2.91021494399851E-4 80-81 0.0010857340367994442 1.5670388159991976E-4 0.0 0.8489320854331653 2.91021494399851E-4 82-83 0.0010969271711994385 1.5670388159991976E-4 0.0 0.9725378686123021 2.91021494399851E-4 84-85 0.0011416997087994154 1.5670388159991976E-4 0.0 1.1166158946090283 2.91021494399851E-4 86-87 0.001164085977599404 1.5670388159991976E-4 0.0 1.3549513053881062 2.91021494399851E-4 88-89 0.001164085977599404 1.5670388159991976E-4 0.0 1.6403202669159602 2.91021494399851E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16395 0.0 22.456032 1 GTATCAA 30395 0.0 22.271807 1 TACATGG 16295 0.0 21.599968 2 AGAGTAC 22490 0.0 21.00342 10-11 GAGTACT 14415 0.0 20.939714 12-13 ACATGGG 16735 0.0 20.180103 3 GTACTTT 15085 0.0 19.757563 14-15 AGTACTT 15160 0.0 19.754028 12-13 TCAACGC 34355 0.0 19.480303 4 CAACGCA 34545 0.0 19.373161 5 ATCAACG 34630 0.0 19.339539 3 TATCAAC 34755 0.0 19.325085 2 AACGCAG 35315 0.0 18.950752 6 GGTATCA 23775 0.0 17.363688 1 TACTTTT 16940 0.0 17.313644 14-15 GAGTACA 13220 0.0 17.284481 1 ACGCAGA 38550 0.0 17.24957 7 CGCAGAG 38695 0.0 16.97626 8 ACTTTTT 17965 0.0 16.748825 16-17 AGTACAT 12625 0.0 16.704782 2 >>END_MODULE