FastQCFastQC Report
Thu 26 May 2016
SRR937114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3942811
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108360.2748293032559765No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100860.25580734151345325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75760.19214717621514193No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62670.15894751232052462No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT49730.12612828766075776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48520.12305941116629733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG140200.023.9659581
TACATGG142100.023.1441332
GTATCAA252400.022.7646161
GAGTACT107000.021.94025812-13
ACATGGG145250.021.398313
TACGTCG1150.003711821320.6646795
AGAGTAC174750.020.60089510-11
GTACTTT112800.020.51643614-15
CAACGCA283850.020.0094645
TATCAAC284650.019.9352652
TCAACGC284900.019.9339494
ATCAACG285100.019.8866253
AACGCAG290650.019.6374456
AGTACTT116100.019.09492712-13
GGTATCA194450.018.3061581
ACGCAGA314200.017.8940017
CATGGGG83800.017.8075224
CGCAGAG314650.017.7029368
ACTTTTT141600.016.76213816-17
GAGTACA110450.016.6951141
Produced by FastQC (version 0.11.5)