FastQCFastQC Report
Thu 26 May 2016
SRR937114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3942811
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117590.29823899750710847No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT115680.29339473791667925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88260.2238504457860141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58560.14852347728562185No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54630.13855596933253966No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT49220.1248347942622662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG144050.022.9243451
TACATGG145100.021.9345172
GTATCAA274450.021.1386781
GAGTACT126100.021.07310912-13
ACATGGG143250.020.9560833
AGAGTAC198600.020.37374510-11
GTACTTT131900.019.98633214-15
AGTACTT131050.019.69727312-13
CAACGCA312450.018.4082585
TCAACGC313300.018.3279954
ATCAACG313400.018.3237743
TATCAAC314450.018.2639792
AACGCAG318400.018.0924876
CATGGGG85250.017.9395084
GGTATCA217200.017.5007041
TACTTTT147600.017.29724714-15
GTATAGC13800.017.2153681
ACTTTTT156650.016.96535116-17
ACGCAGA348100.016.357837
CGCAGAG350150.016.1399948
Produced by FastQC (version 0.11.5)