FastQCFastQC Report
Thu 26 May 2016
SRR937113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110464
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110240.2681935664684084No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102590.24958252888238408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75200.18294771587830475No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63790.15518929249836516No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51620.12558192943667673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47730.11611827764456761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA259750.024.2761971
GTACATG147400.022.8244921
GAGTACT112100.022.39719812-13
TACATGG148850.021.9642052
AGAGTAC184550.021.53452510-11
GTACTTT116050.021.44882214-15
CAACGCA293100.021.1578045
ACATGGG148550.021.1448553
TATCAAC295650.021.1360452
TCAACGC293600.021.1055874
ATCAACG294550.021.1020493
AACGCAG299350.020.7314286
AGTACTT118850.020.22579812-13
ACGCAGA324400.018.880397
CGCAGAG325800.018.6971668
GGTATCA196650.018.1714341
GCAGAGT337100.017.4619719
TACTTTT138050.017.29087414-15
CATGGGA65150.016.9948524
ACTTTTT148150.016.9456416-17
Produced by FastQC (version 0.11.5)