FastQCFastQC Report
Thu 26 May 2016
SRR937113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110464
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121170.29478423847040136No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT119690.29118367172173265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89430.21756667860368073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58470.14224671472612338No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54880.1335129075452309No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT49400.1201810793136736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG152500.022.1206341
TACATGG151150.021.650452
GTATCAA286850.021.085441
GAGTACT128500.020.88238112-13
AGAGTAC202800.020.67171710-11
ACATGGG154400.020.2398853
GTACTTT136450.019.30059214-15
AGTACTT136000.019.18949512-13
CAACGCA327450.018.3806725
TCAACGC328050.018.3138624
ATCAACG329650.018.1961573
TATCAAC331000.018.1947922
AACGCAG335350.017.961846
CATGGGG90000.017.4140934
GAGTACA125000.017.29461
TACTTTT153050.016.8813914-15
AGTACAT119150.016.5029722
GGTATCA227750.016.3138871
ACGCAGA367800.016.209217
ACTTTTT164850.016.1780916-17
Produced by FastQC (version 0.11.5)