FastQCFastQC Report
Thu 26 May 2016
SRR937112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4176643
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110900.26552424997779317No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105470.25252337822504817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77190.18481349734703206No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66660.1596018620696095No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52910.1266806859001356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49110.1175824699405719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG152700.023.0041731
GTATCAA275400.022.109881
TACATGG154600.022.107592
GAGTACT113250.021.23226512-13
AGAGTAC188500.020.80897910-11
ACATGGG157750.020.5210573
GTACTTT122250.019.41683814-15
CAACGCA307600.019.3327225
TCAACGC309300.019.2422924
TATCAAC312550.019.193842
ATCAACG311200.019.1701773
AGTACTT122800.019.03929112-13
AACGCAG315950.018.8332236
CATGGGG94600.018.2409694
ACGCAGA340700.017.2666937
CGCAGAG342500.017.1204538
GGTATCA217350.016.774011
TACTTTT139400.016.51668514-15
ACTTTTT151000.016.17586916-17
GCAGAGT353850.015.8860459
Produced by FastQC (version 0.11.5)