##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937112_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4176643 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55414312403526 31.0 31.0 34.0 30.0 34.0 2 31.64146564597453 33.0 31.0 34.0 30.0 34.0 3 31.66659850985588 33.0 31.0 34.0 30.0 34.0 4 35.26529511859165 37.0 35.0 37.0 33.0 37.0 5 35.25877528914968 37.0 35.0 37.0 33.0 37.0 6 35.31259746164563 37.0 35.0 37.0 33.0 37.0 7 35.247843782674266 37.0 35.0 37.0 32.0 37.0 8 35.204062688623374 37.0 35.0 37.0 32.0 37.0 9 36.79607067206845 39.0 37.0 39.0 33.0 39.0 10-11 36.66244924452485 39.0 37.0 39.0 32.0 39.0 12-13 36.5725143374715 39.0 37.0 39.0 32.0 39.0 14-15 37.68275646254659 40.0 37.0 41.0 32.0 41.0 16-17 37.60716034384552 40.0 37.0 41.0 32.0 41.0 18-19 37.54330954309478 40.0 37.0 41.0 32.0 41.0 20-21 37.45875287880722 40.0 37.0 41.0 32.0 41.0 22-23 37.34496268893463 40.0 37.0 41.0 31.5 41.0 24-25 37.296349843642375 40.0 37.0 41.0 31.0 41.0 26-27 37.31629684414014 40.0 37.0 41.0 31.0 41.0 28-29 37.24474871326086 40.0 37.0 41.0 31.0 41.0 30-31 37.10314108723202 39.5 36.5 41.0 31.0 41.0 32-33 36.93046472968841 39.0 36.0 41.0 30.0 41.0 34-35 36.76522676225859 39.0 36.0 41.0 30.0 41.0 36-37 36.589458088709044 39.0 35.5 41.0 30.0 41.0 38-39 36.42856056885877 39.0 35.0 41.0 30.0 41.0 40-41 36.255822678644066 39.0 35.0 40.5 30.0 41.0 42-43 36.04353304795262 39.0 35.0 40.0 29.0 41.0 44-45 35.81588191760704 39.0 35.0 40.0 28.0 41.0 46-47 35.520684315130595 38.0 35.0 40.0 27.0 41.0 48-49 35.31564332886484 38.5 34.5 40.0 26.0 41.0 50-51 34.633314721895076 38.0 34.0 39.5 25.0 40.5 52-53 34.82168226970799 38.0 34.0 39.5 26.0 40.5 54-55 35.21791089159404 38.5 35.0 40.0 26.0 41.0 56-57 35.081136692793706 38.0 34.0 40.0 26.0 41.0 58-59 34.852086711744334 38.0 34.0 40.0 26.0 41.0 60-61 34.536378139094005 38.0 34.0 40.0 25.5 41.0 62-63 34.21905798508515 37.0 34.0 40.0 25.0 41.0 64-65 33.8733881492864 37.0 33.0 40.0 24.0 41.0 66-67 33.47912390405405 36.0 33.0 39.0 23.0 41.0 68-69 33.069028763052046 36.0 32.5 39.0 22.0 41.0 70-71 32.636281099437994 35.0 32.0 38.5 21.5 40.0 72-73 32.23578158343914 35.0 32.0 37.5 20.5 40.0 74-75 31.846311260023903 35.0 31.0 37.0 20.0 39.0 76-77 31.401713050409143 35.0 31.0 36.5 18.5 39.0 78-79 30.950136988006875 35.0 31.0 36.0 17.5 38.5 80-81 30.524967060866825 34.0 30.5 35.5 13.0 37.0 82-83 30.132672938529822 34.0 30.0 35.0 9.0 37.0 84-85 29.765080592236394 34.0 30.0 35.0 7.0 36.5 86-87 29.433272127878777 34.0 29.0 35.0 4.0 36.0 88-89 29.065180457127887 34.0 29.0 35.0 2.0 36.0 90-91 28.692871404139638 34.0 29.0 35.0 2.0 35.0 92-93 28.305879506579807 34.0 29.0 35.0 2.0 35.0 94-95 27.917982216818626 34.0 27.5 35.0 2.0 35.0 96-97 27.401235633498004 34.0 27.0 35.0 2.0 35.0 98-99 26.816017313426116 33.0 25.5 35.0 2.0 35.0 100-101 24.629277029422912 31.0 20.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3736302479948357 1101 2 0.35851105512008274 1101 3 0.43170040518650765 1101 4 0.35058626442599916 1101 5 0.3509223956834262 1101 6 0.2910001068334793 1101 7 0.25364322246052495 1101 8 0.3471403888884552 1101 9 0.32835808091269314 1101 10-11 0.4081395500012306 1101 12-13 0.4446181637655897 1101 14-15 0.650673870827994 1101 16-17 0.6779252031896235 1101 18-19 0.6767506101663656 1101 20-21 0.6169803236581046 1101 22-23 0.634429220204531 1101 24-25 0.7303382092321442 1101 26-27 0.6734760710602146 1101 28-29 0.6610894529986453 1101 30-31 0.602408581237114 1101 32-33 0.6040409609086765 1101 34-35 0.6177944927687378 1101 36-37 0.5882870739484858 1101 38-39 0.523547303496386 1101 40-41 0.5906165055776 1101 42-43 0.5907238579352239 1101 44-45 0.5563667861489705 1101 46-47 0.5079206351580225 1101 48-49 0.6640043032748721 1101 50-51 0.72232974059974 1101 52-53 0.7138120513619128 1101 54-55 0.659126226248901 1101 56-57 0.6483267871128859 1101 58-59 0.644825650853484 1101 60-61 0.7538197832541371 1101 62-63 0.7909923455483963 1101 64-65 0.6889250012835717 1101 66-67 0.6248980880418813 1101 68-69 0.5953801091889517 1101 70-71 0.669680881685899 1101 72-73 0.6496350808116489 1101 74-75 0.6198622202494342 1101 76-77 0.6929483673622876 1101 78-79 0.7398324910812129 1101 80-81 0.7459990848314 1101 82-83 0.6265242179425847 1101 84-85 0.6188665478794242 1101 86-87 0.7319881977970901 1101 88-89 0.7442187139602829 1101 90-91 0.7166149300271698 1101 92-93 0.7426993213079847 1101 94-95 0.8758401662481248 1101 96-97 0.9088085698037283 1101 98-99 0.9660434700323712 1101 100-101 1.191667613865274 1102 1 0.11065329249702671 1102 2 0.21549821737304597 1102 3 0.22619940130704208 1102 4 0.203692962125686 1102 5 0.27251142925557303 1102 6 0.15592831101468363 1102 7 0.09002703139945112 1102 8 0.040997660522890556 1102 9 0.216275412927736 1102 10-11 0.15501185932321704 1102 12-13 0.0902188548509244 1102 14-15 0.017357371132128208 1102 16-17 0.2556745379247758 1102 18-19 0.05696432929276796 1102 20-21 -0.07602392814036563 1102 22-23 -0.018727940823183076 1102 24-25 -0.026446005584958243 1102 26-27 0.030393138397521113 1102 28-29 -0.1127719879991389 1102 30-31 -0.04179666352464295 1102 32-33 -0.014922137970629024 1102 34-35 0.005438419116330806 1102 36-37 0.043713055179168236 1102 38-39 0.13627544187983176 1102 40-41 -0.03833433577436551 1102 42-43 -0.004488861863599425 1102 44-45 0.04351095683695405 1102 46-47 0.06447253241461226 1102 48-49 0.28862427273796243 1102 50-51 0.2908211176096671 1102 52-53 0.3896527163686869 1102 54-55 0.15301798783915643 1102 56-57 0.2703397769514808 1102 58-59 -0.023174322586868357 1102 60-61 0.008304834725144872 1102 62-63 -0.150166386674492 1102 64-65 0.0876360942205281 1102 66-67 -0.12590661495320177 1102 68-69 0.11119842483798692 1102 70-71 0.09960968596478637 1102 72-73 -0.004039262511533082 1102 74-75 0.12113267754557455 1102 76-77 0.06059901881277696 1102 78-79 0.04433919116960183 1102 80-81 0.14423458888128593 1102 82-83 0.1395006723149912 1102 84-85 -0.00191597029409607 1102 86-87 0.06896723931290794 1102 88-89 0.26428387947239784 1102 90-91 -0.007079076621597125 1102 92-93 0.08391187022304436 1102 94-95 0.23411710000544517 1102 96-97 0.003164135482016661 1102 98-99 0.06803117558879279 1102 100-101 0.0878127359398384 1103 1 0.2702712197747026 1103 2 0.2734091336938036 1103 3 0.2382111226863337 1103 4 0.20836309559491895 1103 5 0.08302440817751489 1103 6 0.20414058075323993 1103 7 0.24998805715839723 1103 8 0.2651625712707997 1103 9 0.15525332469462683 1103 10-11 0.1962936569765361 1103 12-13 0.1408484185316965 1103 14-15 0.35385958500065584 1103 16-17 0.4237899032630068 1103 18-19 0.4011698661026202 1103 20-21 0.284869891308567 1103 22-23 0.36489723441496835 1103 24-25 0.3768457427732699 1103 26-27 0.46847918411697975 1103 28-29 0.436912569189559 1103 30-31 0.4072296434412479 1103 32-33 0.372270319538643 1103 34-35 0.45453735203032863 1103 36-37 0.41383522120067084 1103 38-39 0.4898017601246707 1103 40-41 0.3820179411067173 1103 42-43 0.38697209327050075 1103 44-45 0.33105739964510406 1103 46-47 0.2880343425742282 1103 48-49 0.2644223274055477 1103 50-51 0.2659213729506149 1103 52-53 0.3496119269842666 1103 54-55 0.38756274947687785 1103 56-57 0.41445704685619233 1103 58-59 0.3665934959156161 1103 60-61 0.44020696187104846 1103 62-63 0.4557369204358821 1103 64-65 0.3627702106770556 1103 66-67 0.23848454917141026 1103 68-69 0.3280936257363578 1103 70-71 0.34615737624941545 1103 72-73 0.18954575803596896 1103 74-75 0.20677985377675157 1103 76-77 0.27099952352774537 1103 78-79 0.3292043357192931 1103 80-81 0.21061106708109278 1103 82-83 0.3119081442698146 1103 84-85 0.2892801841245536 1103 86-87 0.31655528164964863 1103 88-89 0.2505945249689141 1103 90-91 0.2581163011259129 1103 92-93 0.2265197817093778 1103 94-95 0.37132791108022545 1103 96-97 0.5306070011155626 1103 98-99 0.5255319180188174 1103 100-101 0.6575448240120529 1104 1 0.17886291828378376 1104 2 0.07199296218680828 1104 3 0.05466687374761747 1104 4 0.14605611879350988 1104 5 0.09736423014331308 1104 6 -0.05383745182422928 1104 7 0.029714323755463568 1104 8 0.03194439295963036 1104 9 0.11445450308404048 1104 10-11 0.2744106464850873 1104 12-13 0.046226004551755295 1104 14-15 -0.08417293574476759 1104 16-17 0.14680198525353205 1104 18-19 0.11016576620889396 1104 20-21 0.13672453770766424 1104 22-23 0.0017171623517739931 1104 24-25 0.050854881865888046 1104 26-27 -0.029345027851867655 1104 28-29 0.030924151210577122 1104 30-31 0.21792949409747564 1104 32-33 -0.12135414061352634 1104 34-35 -0.004337649881946959 1104 36-37 -0.036530153572549295 1104 38-39 0.0213724156974493 1104 40-41 0.025565388699888558 1104 42-43 0.01773343094875912 1104 44-45 0.015169641420563096 1104 46-47 -0.02022813178870564 1104 48-49 -0.06289393000736254 1104 50-51 -0.09502261676448143 1104 52-53 0.006111009268408907 1104 54-55 0.16584826349861004 1104 56-57 0.20955293199573788 1104 58-59 0.08532829626553706 1104 60-61 0.0806861297351773 1104 62-63 -0.10292439713894197 1104 64-65 -0.10821284472257986 1104 66-67 -0.27291820660041566 1104 68-69 -0.22989924175949739 1104 70-71 -0.09495283085747275 1104 72-73 -0.024239895447578874 1104 74-75 -0.09585925184303434 1104 76-77 0.05190870910231027 1104 78-79 0.08994230620419685 1104 80-81 -0.07291012662096463 1104 82-83 -0.13197072834189782 1104 84-85 -0.030388521071863295 1104 86-87 0.1278832796582421 1104 88-89 -0.028237332654850178 1104 90-91 -0.0961809940738263 1104 92-93 -0.07236843422472461 1104 94-95 -0.03919506293186359 1104 96-97 -0.07187031042654723 1104 98-99 -0.11961903087073367 1104 100-101 -0.14289356899640993 1105 1 0.10610681269694311 1105 2 0.19056737092921594 1105 3 0.1762182720827994 1105 4 0.11112161276886212 1105 5 0.1887044655050758 1105 6 0.1412103052508087 1105 7 0.21077617514265512 1105 8 0.1356797824500262 1105 9 0.13237250308967674 1105 10-11 0.1841378450555382 1105 12-13 0.1875979303160804 1105 14-15 0.32862527551987597 1105 16-17 0.41371401775471384 1105 18-19 0.3040957066989307 1105 20-21 0.30297202996526806 1105 22-23 0.23878689173890422 1105 24-25 0.2058761138839742 1105 26-27 0.31712766955158855 1105 28-29 0.2952139770733382 1105 30-31 0.3294013176704169 1105 32-33 0.2457076546294985 1105 34-35 0.034403136845014615 1105 36-37 0.05378545063783946 1105 38-39 0.30710449382578986 1105 40-41 0.31028023167048246 1105 42-43 0.3619205824406393 1105 44-45 0.3170318300323771 1105 46-47 0.24007010491242653 1105 48-49 0.32535307607602704 1105 50-51 0.2084468315912389 1105 52-53 0.33550026895355956 1105 54-55 0.2581273610026855 1105 56-57 0.24026839140810097 1105 58-59 0.28541006898632304 1105 60-61 0.4179562088261335 1105 62-63 0.2896463844610935 1105 64-65 0.37742799708206576 1105 66-67 0.3497538890797145 1105 68-69 0.2701589250744192 1105 70-71 0.28484386431019715 1105 72-73 0.37773822229982557 1105 74-75 0.3353380872010412 1105 76-77 0.3656549723378184 1105 78-79 0.4697067205156671 1105 80-81 0.18442793513591127 1105 82-83 0.18780049110095476 1105 84-85 0.3360927703944796 1105 86-87 0.33878978371547674 1105 88-89 0.38438453932507244 1105 90-91 0.38551657769887626 1105 92-93 0.44561945996386143 1105 94-95 0.4478927580363816 1105 96-97 0.26850655590239114 1105 98-99 0.3415156967967228 1105 100-101 0.40446214058195906 1106 1 0.2956042538487438 1106 2 0.20451981811536868 1106 3 0.2164879686663994 1106 4 0.28578441461915816 1106 5 0.14766437157447143 1106 6 0.25168773571419223 1106 7 0.23528015498331456 1106 8 0.37192734481973844 1106 9 0.438983436060596 1106 10-11 0.4626871630499423 1106 12-13 0.49731920874919666 1106 14-15 0.2938724981255021 1106 16-17 0.35676064418057507 1106 18-19 0.5233988850856974 1106 20-21 0.3552005814731487 1106 22-23 0.23960309972406435 1106 24-25 0.1464417989509883 1106 26-27 0.039527058684093674 1106 28-29 0.0036881930500456406 1106 30-31 0.21935693156393654 1106 32-33 0.24855216124604596 1106 34-35 0.3119303754832714 1106 36-37 0.16595550786684754 1106 38-39 0.008160085068368517 1106 40-41 0.19992430668047945 1106 42-43 0.10565927909149053 1106 44-45 0.06274379061265734 1106 46-47 0.17166351879816233 1106 48-49 0.26182389618156776 1106 50-51 0.291042438901826 1106 52-53 0.3799977941615751 1106 54-55 0.5342620535692859 1106 56-57 0.40034007366498514 1106 58-59 0.4812974194989792 1106 60-61 0.5515840043589435 1106 62-63 0.6158096119012839 1106 64-65 0.27311102462773107 1106 66-67 0.2232466726323068 1106 68-69 0.36993407469448414 1106 70-71 0.3684694699573541 1106 72-73 0.45625588649258475 1106 74-75 0.6370371951433818 1106 76-77 0.5326700520231107 1106 78-79 0.530341866985836 1106 80-81 0.5515302342061581 1106 82-83 0.46729530881398773 1106 84-85 0.5590171127209445 1106 86-87 0.8023760997173 1106 88-89 0.7921769709033164 1106 90-91 0.7893823452294662 1106 92-93 0.9864793860297816 1106 94-95 1.1723634693112395 1106 96-97 1.1715667109551404 1106 98-99 1.1794214671232872 1106 100-101 1.4016751903538562 1107 1 0.18019997241978203 1107 2 0.25251644292813324 1107 3 0.23835599054583412 1107 4 0.1671287983313121 1107 5 0.2091098231737334 1107 6 0.28819721311348445 1107 7 0.2780726086337779 1107 8 0.2460046520210568 1107 9 0.2853925075679271 1107 10-11 0.31728261978707195 1107 12-13 0.40945244367251377 1107 14-15 0.31455554867822144 1107 16-17 0.46986678966932516 1107 18-19 0.45417639839475754 1107 20-21 0.48359058949613143 1107 22-23 0.4757201473837682 1107 24-25 0.5155964558398836 1107 26-27 0.45285084743057524 1107 28-29 0.4413751078759631 1107 30-31 0.5445482926259757 1107 32-33 0.5173864668230124 1107 34-35 0.32167086844240345 1107 36-37 0.4962831361852267 1107 38-39 0.4990452269329424 1107 40-41 0.651674024097467 1107 42-43 0.7618471552924646 1107 44-45 0.645764677053819 1107 46-47 0.6510200891494762 1107 48-49 0.6790848517073442 1107 50-51 0.610683267008362 1107 52-53 0.6436339309949872 1107 54-55 0.7643401739377893 1107 56-57 0.8382220794510147 1107 58-59 0.8656046761515341 1107 60-61 0.8659396162993644 1107 62-63 0.9566866023329794 1107 64-65 0.9724564151653468 1107 66-67 0.8962908721714768 1107 68-69 0.8823491268834118 1107 70-71 0.9048804138241238 1107 72-73 0.8424130243812371 1107 74-75 0.8937812707807744 1107 76-77 0.9277182047602146 1107 78-79 0.8709164392579218 1107 80-81 0.9053717328051007 1107 82-83 0.8842406309783755 1107 84-85 0.8584311976065067 1107 86-87 0.897855759669163 1107 88-89 0.7537791523255564 1107 90-91 0.7906304284076775 1107 92-93 0.932816700226514 1107 94-95 1.0528394537079144 1107 96-97 0.9590649571620062 1107 98-99 1.1595346880557713 1107 100-101 1.4304715843575657 1108 1 0.029886058921825054 1108 2 0.019211435078073436 1108 3 0.060716027917891324 1108 4 0.029963043008315537 1108 5 0.04901375218664583 1108 6 0.05817628741723979 1108 7 0.018430512196403015 1108 8 0.05841032530956625 1108 9 -0.005673388129395107 1108 10-11 -0.07946657512488997 1108 12-13 0.026353695651387454 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58-59 -1.7372606945361113 2316 60-61 -2.002226302658599 2316 62-63 -2.009875695116264 2316 64-65 -2.565567703442447 2316 66-67 -2.2194342484203275 2316 68-69 -2.160027548112531 2316 70-71 -1.9289811879373815 2316 72-73 -2.249418455612677 2316 74-75 -2.656411269768899 2316 76-77 -2.7853672286786413 2316 78-79 -2.993726115470306 2316 80-81 -2.9202077043903323 2316 82-83 -3.574892191186013 2316 84-85 -3.3491791592088838 2316 86-87 -3.6439506546686644 2316 88-89 -3.3088372834826494 2316 90-91 -3.6768457249459736 2316 92-93 -3.7555162280053054 2316 94-95 -3.9233602148992865 2316 96-97 -4.150198639922053 2316 98-99 -4.428171734631096 2316 100-101 -4.448544107891756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8797.0 3 3474.0 4 3432.0 5 3938.0 6 4464.0 7 5144.0 8 6544.0 9 8133.0 10 10082.0 11 12360.0 12 14897.0 13 17636.0 14 19254.0 15 22489.0 16 26980.0 17 33443.0 18 30523.0 19 22883.0 20 23700.0 21 26178.0 22 28677.0 23 32270.0 24 37560.0 25 42826.0 26 50415.0 27 59761.0 28 71494.0 29 85618.0 30 105006.0 31 129403.0 32 162246.0 33 209398.0 34 279809.0 35 391646.0 36 579787.0 37 820710.0 38 677091.0 39 108536.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81505980334368 18.4251830075488 14.54403138989931 23.21572579920821 2 15.299782682522492 20.063212227257466 40.249716318545175 24.387288771674868 3 19.525931013639354 24.21743077762746 29.40689049359415 26.84974771513903 4 13.000627832101264 16.56020349329518 37.37859856664566 33.0605701079579 5 14.240587629113044 36.91540249718408 33.961272078031016 14.882737795671867 6 31.94029371354827 35.61005916649169 17.761524809945264 14.688122310014776 7 28.509600734404916 32.23670088906691 21.00631392743727 18.247384449090905 8 26.162631870544008 33.985337279944176 20.099414526416286 19.752616323095534 9 26.087393009776385 17.207542884142313 20.507908105871415 36.19715600020989 10-11 24.861484370966114 26.207412186979358 27.490253380363576 21.440850061690952 12-13 26.04486550244518 23.608644951900214 27.742380356703457 22.60410918895115 14-15 23.115991194957704 24.99081210894563 26.129164258888178 25.76403243720849 16-17 22.390652744798015 28.089040870286475 26.580771891160293 22.93953449375522 18-19 22.301983506950684 27.22846864562176 28.49420788371782 21.975339963709732 20-21 23.335033021747748 26.047635115347035 28.351198028792652 22.266133834112566 22-23 22.884503485446103 25.96498375382503 28.381677873671308 22.768834887057558 24-25 22.93420594373248 26.1011380578632 28.36677795785557 22.597878040548753 26-27 22.671454221609697 26.15125069345961 28.57947867161273 22.597816413317968 28-29 22.45562706115345 26.693524964185343 28.31905416267695 22.531793811984254 30-31 22.74679349886039 26.13569892231105 28.227362222870834 22.89014535595772 32-33 22.617392053903014 26.23228434126123 28.300074945108094 22.850248659727658 34-35 23.106071444434452 26.208759574832598 28.11046699909982 22.57470198163313 36-37 22.992883106770638 26.060416612293697 28.289141775418596 22.65755850551707 38-39 22.713336525799114 26.42272237519454 28.188135999042256 22.675805099964087 40-41 22.851820063722673 26.330910859731706 28.099237174025294 22.71803190252033 42-43 22.645478841465547 26.32574551336783 27.82636157679675 23.202414068369873 44-45 22.882286266483675 26.186611648449105 27.801315246341723 23.129786838725494 46-47 23.25619063786262 26.541694669980693 27.525368189208944 22.67674650294774 48-49 23.06030406303258 26.507110749948403 27.486966777553405 22.945618409465613 50-51 22.778120297421445 26.68457213044691 27.62207699389331 22.915230578238337 52-53 23.07378824379004 26.84621457203422 27.073419983780276 23.006577200395462 54-55 22.907792154459287 26.572957963741167 27.217567969300088 23.301681912499458 56-57 23.003077001173168 26.720045571367905 27.129821530100816 23.147055897358108 58-59 23.331226273125285 26.508924477455054 27.14771090655068 23.01213834286898 60-61 23.04989305774403 26.618374051520476 27.534847516600415 22.79688537413508 62-63 22.764328190417583 26.697419602781892 27.61630823493072 22.921943971869805 64-65 22.800117273924762 26.86023009680366 27.24716461315587 23.092488016115706 66-67 22.83543217357996 26.73492731919243 27.036752724085932 23.392887783141674 68-69 22.815006600931618 26.630228901361374 27.342839680898884 23.21192481680813 70-71 23.138717843094533 26.49880295560425 27.098996116538114 23.263483084763102 72-73 23.011179911260815 26.468349523489593 27.28097796508998 23.239492600159608 74-75 22.792042712870217 27.10664795849401 26.92488622381106 23.176423104824714 76-77 22.99253091006798 26.642061065539714 26.913713320000777 23.451694704391528 78-79 22.914194477127573 26.79037612884515 27.01551650929671 23.27991288473057 80-81 23.07152834849614 26.76605751882672 26.858016719419016 23.30439741325813 82-83 23.052785067974355 26.655723488528405 27.178114952945254 23.11337649055199 84-85 22.922306770231458 26.846477917453072 26.964755468735408 23.266459843580066 86-87 22.92758906395327 26.86537012339822 27.00117797534574 23.205862837302767 88-89 22.954790899421194 26.674925141354418 26.814815382608792 23.5554685766156 90-91 22.940084640045068 26.473811312294366 27.05583138675921 23.530272660901357 92-93 23.077322906466712 26.62037924182295 27.02696811578651 23.275329735923826 94-95 22.952529813661993 26.53637114110553 27.09836039076685 23.412738654465628 96-97 22.59820449331654 26.59535007351568 27.40144828279829 23.404997150369493 98-99 22.68814483331563 26.78907843632962 26.91886095570089 23.603915774653856 100-101 22.737603018653815 26.532988012984738 26.94978286892874 23.779626099432708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 346.0 1 260.5 2 225.5 3 281.5 4 288.0 5 337.0 6 509.0 7 1031.0 8 1847.0 9 2351.5 10 2240.5 11 2105.5 12 2293.0 13 2528.5 14 2766.0 15 3038.5 16 3327.5 17 3724.5 18 4118.5 19 4450.0 20 4985.5 21 5766.5 22 6938.5 23 8590.0 24 10585.0 25 13724.0 26 18438.5 27 23855.5 28 28768.0 29 34546.5 30 41574.5 31 50091.0 32 59275.5 33 66631.0 34 73736.5 35 80983.5 36 90028.0 37 99492.0 38 105988.0 39 110959.0 40 116091.0 41 121069.5 42 124897.5 43 130913.0 44 138045.0 45 144474.0 46 150719.5 47 162732.0 48 181707.5 49 182941.5 50 167244.0 51 159336.0 52 158791.0 53 157608.0 54 154931.0 55 146307.0 56 133314.5 57 121317.0 58 111044.0 59 98699.5 60 82518.0 61 67680.0 62 53655.0 63 40360.0 64 28912.0 65 20143.0 66 14262.0 67 10413.0 68 7560.5 69 5575.0 70 4020.5 71 2409.5 72 1491.5 73 958.5 74 589.5 75 367.5 76 227.0 77 117.0 78 75.0 79 62.5 80 46.5 81 31.0 82 22.5 83 15.0 84 13.0 85 13.0 86 11.5 87 10.0 88 8.0 89 7.0 90 9.0 91 7.0 92 3.0 93 3.0 94 3.0 95 1.5 96 0.5 97 2.0 98 2.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03691960265696637 2 0.028372068189692054 3 0.024086329619266 4 0.04692764021248644 5 0.09440596191726226 6 0.11221931105914486 7 0.05695962044158431 8 0.08645699428943293 9 0.0721632181634868 10-11 0.07222307484743129 12-13 0.07000837754148488 14-15 0.06395088112630168 16-17 0.05160843289694618 18-19 0.060359480089631795 20-21 0.056229368897461426 22-23 0.032382466013973425 24-25 0.008655276498374413 26-27 0.0028731208293359045 28-29 0.006703948601783777 30-31 0.004932190757026636 32-33 0.0060574964151831985 34-35 0.00633283716132789 36-37 0.005794127005827408 38-39 0.003423802321625286 40-41 0.00633283716132789 42-43 0.0029928341972249007 44-45 0.0020830126012685306 46-47 0.0012929043732011571 48-49 2.154840622001928E-4 50-51 0.001867528539068338 52-53 0.005889897700138604 54-55 0.011360798612665722 56-57 0.008056709658929432 58-59 0.004980076104182235 60-61 0.002154840622001928 62-63 0.008332050405074122 64-65 0.0023104680002576233 66-67 0.021177294779563396 68-69 0.044377745476450826 70-71 0.057546215944240385 72-73 0.0032083182594250933 74-75 0.013683237949712245 76-77 0.021548406220019283 78-79 0.024565183090821983 80-81 0.07309698243302097 82-83 0.027162963174013195 84-85 0.010750260436431843 86-87 0.030131854697660297 88-89 0.04648470075129715 90-91 0.042785557683527174 92-93 0.042785557683527174 94-95 0.011863594757799505 96-97 0.015873992582080873 98-99 0.03786533826328944 100-101 0.07486874027777811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4176643.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.97157830569036 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37337201617406 36.889849989692756 2 14.53855831365887 14.821065270730172 3 5.285134511149072 8.081749427734243 4 2.6010009892985524 5.30308502396837 5 1.4023297483047572 3.5739480288057486 6 0.8511788958170934 2.6031559044175223 7 0.5659301520149461 2.0192447141286753 8 0.40962484053932 1.67033797084047 9 0.29296051904815773 1.3439394033424976 >10 1.5376022391848816 13.974026929098022 >50 0.09693373781423338 3.378836615793927 >100 0.04288251381998121 3.87661425759426 >500 0.001316276484951222 0.4459507203470069 >1k 9.401973528579333E-4 1.028267208950762 >5k 1.41029602928685E-4 0.4714698483749644 >10k+ 9.401973528578999E-5 0.5184586861805807 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11090 0.26552424997779317 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10547 0.25252337822504817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7719 0.18481349734703206 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6666 0.1596018620696095 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5291 0.1266806859001356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4911 0.1175824699405719 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.788534715559841E-5 2 0.0 0.0 0.0 0.0 4.788534715559841E-5 3 0.0 0.0 0.0 0.0 4.788534715559841E-5 4 0.0 0.0 0.0 2.3942673577799205E-5 4.788534715559841E-5 5 0.0 4.788534715559841E-5 0.0 2.3942673577799205E-5 4.788534715559841E-5 6 0.0 4.788534715559841E-5 0.0 9.577069431119682E-5 4.788534715559841E-5 7 7.182802073339761E-5 4.788534715559841E-5 0.0 1.1971336788899602E-4 4.788534715559841E-5 8 7.182802073339761E-5 4.788534715559841E-5 0.0 1.6759871504459442E-4 4.788534715559841E-5 9 7.182802073339761E-5 4.788534715559841E-5 0.0 1.6759871504459442E-4 7.182802073339761E-5 10-11 7.182802073339761E-5 4.788534715559841E-5 0.0 1.6759871504459442E-4 7.182802073339761E-5 12-13 9.577069431119682E-5 4.788534715559841E-5 0.0 2.6336940935579124E-4 7.182802073339761E-5 14-15 1.4365604146679523E-4 5.985668394449801E-5 0.0 2.6336940935579124E-4 7.182802073339761E-5 16-17 1.6759871504459442E-4 7.182802073339761E-5 0.0 2.6336940935579124E-4 7.182802073339761E-5 18-19 2.0351272541129325E-4 7.182802073339761E-5 0.0 3.9505411403368683E-4 7.182802073339761E-5 20-21 2.1548406220019283E-4 7.182802073339761E-5 0.0 4.4293946118928527E-4 7.182802073339761E-5 22-23 2.1548406220019283E-4 7.182802073339761E-5 0.0 5.626528290782814E-4 8.379935752229721E-5 24-25 2.1548406220019283E-4 7.182802073339761E-5 0.0 6.823661969672773E-4 9.577069431119682E-5 26-27 2.1548406220019283E-4 7.182802073339761E-5 0.0 8.619362488007714E-4 9.577069431119682E-5 28-29 3.3519743008918884E-4 8.379935752229721E-5 0.0 0.0015682451193458479 9.577069431119682E-5 30-31 3.5914010366698806E-4 1.3168470467789562E-4 0.0 0.0025379233992467156 9.577069431119682E-5 32-33 3.7111144045588767E-4 1.6759871504459442E-4 0.0 0.003747028414925575 9.577069431119682E-5 34-35 4.1899678761148605E-4 1.7957005183349403E-4 0.0 0.005183588829593528 1.1971336788899602E-4 36-37 4.3096812440038566E-4 1.9154138862239364E-4 0.0 0.006895489990406171 1.1971336788899602E-4 38-39 4.788534715559841E-4 1.9154138862239364E-4 0.0 0.008344021741863022 1.1971336788899602E-4 40-41 4.788534715559841E-4 1.9154138862239364E-4 0.0 0.010750260436431843 1.1971336788899602E-4 42-43 4.788534715559841E-4 1.9154138862239364E-4 0.0 0.014186034094846027 1.1971336788899602E-4 44-45 4.788534715559841E-4 1.9154138862239364E-4 0.0 0.019357651587650654 1.1971336788899602E-4 46-47 5.746241658671808E-4 1.9154138862239364E-4 0.0 0.03250217938186242 1.1971336788899602E-4 48-49 6.225095130227792E-4 1.9154138862239364E-4 0.0 0.058096897436529765 1.1971336788899602E-4 50-51 6.225095130227792E-4 2.3942673577799205E-4 0.0 0.07626938668207936 1.1971336788899602E-4 52-53 6.225095130227792E-4 2.8731208293359046E-4 0.0 0.09571083762725231 1.3168470467789562E-4 54-55 6.464521866005785E-4 2.8731208293359046E-4 0.0 0.12281394411732101 1.4365604146679523E-4 56-57 7.182802073339761E-4 2.9928341972249007E-4 0.0 0.1437158981507397 1.4365604146679523E-4 58-59 7.182802073339761E-4 3.112547565113896E-4 0.0 0.1715612275217202 1.4365604146679523E-4 60-61 7.182802073339761E-4 3.3519743008918884E-4 0.0 0.21730370539210558 1.4365604146679523E-4 62-63 7.182802073339761E-4 3.3519743008918884E-4 0.0 0.24723204736435458 1.4365604146679523E-4 64-65 7.182802073339761E-4 3.5914010366698806E-4 0.0 0.28509738562764403 1.4365604146679523E-4 66-67 7.901082280673738E-4 3.5914010366698806E-4 0.0 0.3574042598325976 1.556273782556948E-4 68-69 8.379935752229721E-4 3.5914010366698806E-4 0.0 0.44030576709572733 1.6759871504459442E-4 70-71 8.619362488007713E-4 3.5914010366698806E-4 0.0 0.5080036766369547 1.6759871504459442E-4 72-73 8.858789223785705E-4 3.5914010366698806E-4 0.0 0.5843209486661896 1.9154138862239364E-4 74-75 9.098215959563698E-4 3.5914010366698806E-4 0.0 0.6515639474094386 1.9154138862239364E-4 76-77 9.098215959563698E-4 3.5914010366698806E-4 0.0 0.7048723101304086 1.9154138862239364E-4 78-79 9.098215959563698E-4 3.5914010366698806E-4 0.0 0.7729652737856696 1.9154138862239364E-4 80-81 9.457356063230686E-4 3.5914010366698806E-4 0.0 0.8692746782523668 1.9154138862239364E-4 82-83 0.0010055922902675665 3.5914010366698806E-4 0.0 0.9910351447322646 2.1548406220019283E-4 84-85 0.0010175636270564662 3.5914010366698806E-4 0.0 1.1365467434013392 2.1548406220019283E-4 86-87 0.0010295349638453659 3.5914010366698806E-4 0.0 1.3824260297085482 2.1548406220019283E-4 88-89 0.0010295349638453659 3.5914010366698806E-4 0.0 1.6807158284775596 2.1548406220019283E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15270 0.0 23.004173 1 GTATCAA 27540 0.0 22.10988 1 TACATGG 15460 0.0 22.10759 2 GAGTACT 11325 0.0 21.232265 12-13 AGAGTAC 18850 0.0 20.808979 10-11 ACATGGG 15775 0.0 20.521057 3 GTACTTT 12225 0.0 19.416838 14-15 CAACGCA 30760 0.0 19.332722 5 TCAACGC 30930 0.0 19.242292 4 TATCAAC 31255 0.0 19.19384 2 ATCAACG 31120 0.0 19.170177 3 AGTACTT 12280 0.0 19.039291 12-13 AACGCAG 31595 0.0 18.833223 6 CATGGGG 9460 0.0 18.240969 4 ACGCAGA 34070 0.0 17.266693 7 CGCAGAG 34250 0.0 17.120453 8 GGTATCA 21735 0.0 16.77401 1 TACTTTT 13940 0.0 16.516685 14-15 ACTTTTT 15100 0.0 16.175869 16-17 GCAGAGT 35385 0.0 15.886045 9 >>END_MODULE