FastQCFastQC Report
Thu 26 May 2016
SRR937112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4176643
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122190.2925555284471285No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120230.2878627644258798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91670.2194824886876853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58060.13901116279270218No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57370.13735911831583403No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51190.12256254604475411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG155150.022.3260421
GTATCAA286600.022.2656731
TACATGG158250.021.2217732
AGAGTAC214500.020.918110-11
GAGTACT137000.020.63126212-13
ACATGGG157050.020.4455663
AGTACTT143500.020.0939912-13
GTACTTT141350.019.709514-15
ATCAACG328550.019.141543
TCAACGC328700.019.1183554
CAACGCA330500.019.0429745
TATCAAC331850.018.9948162
AACGCAG338400.018.6276026
TATTCCG4350.018.563085
TACTTTT160100.017.6682614-15
CATGGGG97900.017.6607484
ATTCCGT5150.017.525196
ACGCAGA370150.016.95287
CATGGGA67000.016.8730094
GGTATCA223000.016.8115631
Produced by FastQC (version 0.11.5)