FastQCFastQC Report
Thu 26 May 2016
SRR937111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556565
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154900.9951399395463729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109300.7021871878141934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73720.4736069486336902No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA61890.3976062676470305No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA50180.32237651495440284No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT40810.2621798639954001No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT24390.15669117576201444No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.14319993061645353No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG22030.1415295859793841No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA21120.13568337974964104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20550.13202147035298878No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC18180.11679563654585579No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG18150.11660290447234777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17010.10927908567904328No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16750.10760874104197385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGC908.9294603E-426.3987566
GTATCAA215700.026.2481861
GTACATG128050.025.3187621
TACATGG128850.024.7188382
GAGTACT108250.024.3162112-13
ACATGGG129500.023.817753
AGTACTT110600.023.67066812-13
TATCACG1252.2443286E-422.818072
TATCAAC253650.022.4147552
CATGGGA92150.022.2283784
ATCAACG253550.022.1928923
TAGGTCG1503.0246047E-522.1756695
GTACTTT120000.022.0943414-15
TCAACGC255150.022.0537264
CAACGCA256300.021.9148655
ATGGGAG33000.021.8876725
AACGCAG259550.021.6580666
CTATACG1100.002868901521.6024574
GGTATCA157250.021.584661
ATCACGC1353.783089E-421.1224043
Produced by FastQC (version 0.11.5)