FastQCFastQC Report
Thu 26 May 2016
SRR937111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556565
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164001.0536020018438035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122160.7848050033246283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88290.5672104923340817No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA67100.431077404412922No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA51430.33040701801723665No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33480.21508899403494233No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25180.16176645369772544No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.15617722356599306No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG24270.1559202474679824No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT23500.15097345758127673No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.14101563378336274No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21580.1386386048767639No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA20590.13227844645099948No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19270.1237982352166469No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG19040.12232062265308548No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC17510.11249128690417683No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG16590.10658083664993111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG856.4108684E-427.9391945
GTATCAA230900.024.298891
GGTATCA172900.024.207021
GAGTACT128850.023.24071512-13
GTACATG136300.022.6557241
AGTACTT129350.022.54502912-13
TACATGG138150.022.1761192
GTACTTT139150.021.5566314-15
ACATGGG138400.021.003523
TATCAAC277800.020.19272
ATCAACG278600.020.1688143
TCAACGC279850.020.0611084
CAACGCA281850.019.9019035
AACGCAG286800.019.574346
ACTTTTT155500.019.55033516-17
TACTTTT150950.019.39950614-15
CATGGGA98550.019.3263824
ATAGCGT1507.728097E-418.998046
ATGGGAG36450.018.6337935
GGACCGC1801.2346526E-418.4703186
Produced by FastQC (version 0.11.5)