##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937111_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1556565 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97949908934095 31.0 31.0 34.0 30.0 34.0 2 31.943562909354895 31.0 31.0 34.0 30.0 34.0 3 32.107198221725405 33.0 31.0 34.0 30.0 34.0 4 35.89336712569022 37.0 35.0 37.0 35.0 37.0 5 35.8240786603836 37.0 35.0 37.0 35.0 37.0 6 35.88292425950731 37.0 35.0 37.0 35.0 37.0 7 35.80958906309727 37.0 35.0 37.0 35.0 37.0 8 35.80310362882373 37.0 35.0 37.0 33.0 37.0 9 37.431189189015555 39.0 37.0 39.0 34.0 39.0 10-11 37.3563413670486 39.0 37.0 39.0 34.0 39.0 12-13 37.254064558820225 39.0 37.0 39.0 33.5 39.0 14-15 38.32239610938188 40.0 38.0 41.0 33.5 41.0 16-17 38.27328733461179 40.0 38.0 41.0 33.0 41.0 18-19 38.4222380690816 40.0 38.0 41.0 33.5 41.0 20-21 38.525427784898156 40.0 38.0 41.0 34.0 41.0 22-23 38.453760363364204 40.0 38.0 41.0 34.0 41.0 24-25 38.33789112565168 40.0 38.0 41.0 34.0 41.0 26-27 38.1301982249376 40.0 37.0 41.0 33.5 41.0 28-29 37.9322071355838 40.0 37.0 41.0 33.0 41.0 30-31 37.695020124440674 39.0 37.0 41.0 33.0 41.0 32-33 37.561322848708535 40.0 36.0 41.0 33.0 41.0 34-35 37.425166632938556 40.0 36.0 41.0 33.0 41.0 36-37 37.18451237179302 40.0 35.5 41.0 32.0 41.0 38-39 36.970466700716 39.0 35.0 41.0 31.5 41.0 40-41 36.713441134806445 39.0 35.0 41.0 31.0 41.0 42-43 36.400140051973416 39.0 35.0 41.0 30.5 41.0 44-45 35.90728495115847 39.0 35.0 41.0 29.5 41.0 46-47 35.39557422915201 39.0 35.0 41.0 26.5 41.0 48-49 34.88887518349699 39.0 35.0 40.0 24.0 41.0 50-51 34.55927956750923 38.5 34.5 40.0 23.0 41.0 52-53 34.25874473600524 38.0 34.0 40.0 22.0 41.0 54-55 34.01905092302602 38.0 34.0 40.0 20.5 41.0 56-57 33.76774532383807 38.0 33.5 40.0 19.5 41.0 58-59 33.45777914831697 38.0 33.0 40.0 18.0 41.0 60-61 33.11997282477763 37.0 33.0 40.0 15.5 41.0 62-63 32.74789456270699 37.0 32.0 39.5 13.0 41.0 64-65 32.32728090378494 36.0 32.0 39.0 8.0 40.5 66-67 31.94980421633533 36.0 31.0 39.0 7.5 40.0 68-69 31.53265298911385 35.0 31.0 38.5 6.5 40.0 70-71 31.063718187162117 35.0 30.5 38.0 2.0 40.0 72-73 30.73085479886802 35.0 30.0 37.0 2.0 39.0 74-75 30.302336876391287 34.5 30.0 37.0 2.0 39.0 76-77 28.89412488395923 33.0 28.5 35.0 2.0 37.5 78-79 29.584904581562608 34.0 29.0 36.0 2.0 38.0 80-81 29.436363402749002 34.0 29.0 35.5 2.0 37.0 82-83 29.11194585513615 34.0 29.0 35.0 2.0 37.0 84-85 28.745957605368233 34.0 29.0 35.0 2.0 36.0 86-87 28.367841689874822 34.0 29.0 35.0 2.0 36.0 88-89 27.98133903820271 34.0 28.0 35.0 2.0 35.5 90-91 27.553997102594494 33.5 27.0 35.0 2.0 35.0 92-93 27.133697596952263 33.0 26.0 35.0 2.0 35.0 94-95 26.685776694195233 33.0 25.5 35.0 2.0 35.0 96-97 26.062826480102018 33.0 24.5 35.0 2.0 35.0 98-99 25.355255964254624 32.0 21.5 34.0 2.0 35.0 100-101 22.571594504566143 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35640400786661885 1101 2 0.266576517881024 1101 3 0.28168178754263806 1101 4 0.2497523141606024 1101 5 0.3134844494267526 1101 6 0.2745892417371465 1101 7 0.4273328018821587 1101 8 0.3579973073731466 1101 9 0.45021669096159656 1101 10-11 0.35959860789206033 1101 12-13 0.5214831109514222 1101 14-15 0.5702024015756493 1101 16-17 0.6703184335697259 1101 18-19 0.6273784388094867 1101 20-21 0.6514866605929015 1101 22-23 0.3484937554751326 1101 24-25 0.5506771525503282 1101 26-27 0.493335284654151 1101 28-29 0.5711713160037775 1101 30-31 0.6259965726470966 1101 32-33 0.7237836294534077 1101 34-35 0.7512024235904704 1101 36-37 0.7481033482426866 1101 38-39 0.8001543837275662 1101 40-41 0.5289819567818483 1101 42-43 0.6082647712896119 1101 44-45 0.6321703649153747 1101 46-47 0.7969221147820136 1101 48-49 0.6747231127662872 1101 50-51 0.7264704012815102 1101 52-53 0.5352718945367201 1101 54-55 0.6552693506033904 1101 56-57 0.7966477206059324 1101 58-59 0.8708972534613153 1101 60-61 0.7532669302765456 1101 62-63 0.7836791961015379 1101 64-65 0.6768480054080825 1101 66-67 0.6451133172409733 1101 68-69 0.6128412693849405 1101 70-71 0.40418702293270314 1101 72-73 0.51366979515943 1101 74-75 0.6560412029357501 1101 76-77 0.4347881149258441 1101 78-79 0.5030749666486791 1101 80-81 0.6898847575016127 1101 82-83 0.7998052660472901 1101 84-85 0.7825971518786616 1101 86-87 0.7626484586340538 1101 88-89 0.8458731917765263 1101 90-91 0.9095246816348102 1101 92-93 1.0261587156319685 1101 94-95 1.024493687517193 1101 96-97 1.0453823181552906 1101 98-99 1.2013977138409864 1101 100-101 1.5420399136113083 1102 1 0.2265736144185233 1102 2 0.13156849478370347 1102 3 0.1953759781300235 1102 4 0.19011108176684388 1102 5 0.1965298331263483 1102 6 0.23061317874864073 1102 7 0.20799742491161055 1102 8 0.2843760435771614 1102 9 0.45006140520705173 1102 10-11 0.40134675165216294 1102 12-13 0.34976797979247465 1102 14-15 0.463580039276728 1102 16-17 0.3688023844994248 1102 18-19 0.38725849494240805 1102 20-21 0.2450420142130767 1102 22-23 0.239091346820544 1102 24-25 0.3958745092417004 1102 26-27 0.3044071589532038 1102 28-29 0.40567049068874894 1102 30-31 0.5236661360640724 1102 32-33 0.6366309375460943 1102 34-35 0.7053643693784863 1102 36-37 0.6833779452186448 1102 38-39 0.8528861189596029 1102 40-41 0.9465101238923239 1102 42-43 1.0109796838982348 1102 44-45 1.205712548003028 1102 46-47 1.2044170967516195 1102 48-49 1.072606194095762 1102 50-51 1.19118602705435 1102 52-53 1.2285293295611268 1102 54-55 1.1587000155139862 1102 56-57 1.2286641923985542 1102 58-59 1.1210769823438937 1102 60-61 1.15856418747002 1102 62-63 1.3987991399686166 1102 64-65 1.1762894080410362 1102 66-67 1.1202675965137736 1102 68-69 0.811165621066916 1102 70-71 0.8961639256145446 1102 72-73 1.0042174937670332 1102 74-75 0.8549047412654538 1102 76-77 0.6794278807319181 1102 78-79 0.8607462267063077 1102 80-81 0.8384738138818442 1102 82-83 0.926276886137817 1102 84-85 0.848469944089068 1102 86-87 0.7186716767300183 1102 88-89 0.8115075915970849 1102 90-91 1.0838056223214032 1102 92-93 0.8527677923718855 1102 94-95 0.9286708743144523 1102 96-97 1.1587100056053288 1102 98-99 1.3391476807708678 1102 100-101 1.575621176947191 1103 1 -0.002525421312590481 1103 2 -0.19108000935623437 1103 3 0.02101627107872872 1103 4 0.08684096146334497 1103 5 0.08762645372040367 1103 6 0.1270095689567583 1103 7 0.1944167438099882 1103 8 0.25448151005717534 1103 9 0.1529325745427812 1103 10-11 0.20325639969882303 1103 12-13 0.23816440022414298 1103 14-15 0.21178517206118386 1103 16-17 0.26482784922097835 1103 18-19 0.1947155397103657 1103 20-21 0.2890686370464195 1103 22-23 0.3225124887489983 1103 24-25 0.2308219408574672 1103 26-27 0.22584084777969338 1103 28-29 0.2845956035806054 1103 30-31 0.33690274943194964 1103 32-33 0.3570449934239619 1103 34-35 0.31367117696019875 1103 36-37 0.3465559660976325 1103 38-39 0.29493313307812485 1103 40-41 0.41657128538366095 1103 42-43 0.29034685067320964 1103 44-45 0.3916423826389561 1103 46-47 0.3098190082542871 1103 48-49 0.452560796937604 1103 50-51 0.30926294326227577 1103 52-53 0.34893414664678346 1103 54-55 0.47674938707004344 1103 56-57 0.3977667175768431 1103 58-59 0.4131598525927913 1103 60-61 0.5943796534142578 1103 62-63 0.54022873487677 1103 64-65 0.5595849161333959 1103 66-67 0.552615939507195 1103 68-69 0.5132049556540643 1103 70-71 0.37728673766935117 1103 72-73 0.4192571827940945 1103 74-75 0.2774668335050059 1103 76-77 0.5321158540912272 1103 78-79 0.6434201255040968 1103 80-81 0.7139005499161577 1103 82-83 0.731545470535039 1103 84-85 0.6783387875846145 1103 86-87 0.4875457736294919 1103 88-89 0.7522656396497922 1103 90-91 1.0769563801771262 1103 92-93 1.0425834149291013 1103 94-95 1.2052854648745353 1103 96-97 1.2819438164250876 1103 98-99 1.2010680925256345 1103 100-101 1.2492210019253136 1104 1 0.2870869854795579 1104 2 0.18123684955446961 1104 3 0.24085700631171392 1104 4 0.12714160752367576 1104 5 0.07450319829327867 1104 6 0.14404318082652168 1104 7 0.2230375768002375 1104 8 0.20300005539796473 1104 9 0.22685038133050028 1104 10-11 0.29366764353608943 1104 12-13 0.27346954526117173 1104 14-15 0.30516613733887965 1104 16-17 0.3866198275174213 1104 18-19 0.359160660957869 1104 20-21 0.28068691615229113 1104 22-23 0.35449424416670183 1104 24-25 0.3943252697033941 1104 26-27 0.3114629383670291 1104 28-29 0.335541513017084 1104 30-31 0.4297152662762542 1104 32-33 0.342383223548687 1104 34-35 0.464332167740352 1104 36-37 0.3033355321125484 1104 38-39 0.25707840257195613 1104 40-41 0.2580573398786541 1104 42-43 0.40875211307052695 1104 44-45 0.488962970029057 1104 46-47 0.5876595060550684 1104 48-49 0.7659699961462039 1104 50-51 0.6587239754442678 1104 52-53 0.5093144813071575 1104 54-55 0.44897990398775534 1104 56-57 0.3990102605284278 1104 58-59 0.4727475670960857 1104 60-61 0.5112625057489382 1104 62-63 0.6759678716089539 1104 64-65 0.5303223878639187 1104 66-67 0.6896655830116565 1104 68-69 0.7699826036928918 1104 70-71 0.7161053173022296 1104 72-73 0.764419964401764 1104 74-75 0.68820174572949 1104 76-77 0.6032489579949392 1104 78-79 0.4944519139738226 1104 80-81 0.723675944994433 1104 82-83 0.7389648965244753 1104 84-85 0.9082794857477552 1104 86-87 0.693322783775919 1104 88-89 0.8916752204695015 1104 90-91 0.6882811692576247 1104 92-93 0.8079793893618188 1104 94-95 0.911992528086067 1104 96-97 1.0745302435696047 1104 98-99 1.0764188258704088 1104 100-101 1.086135328909151 1105 1 0.09679657633611427 1105 2 -0.08135062997160247 1105 3 0.030812355253260648 1105 4 0.04743460149091305 1105 5 0.10206090077812746 1105 6 0.08897773192771297 1105 7 0.11669979512051043 1105 8 0.11787362141700441 1105 9 0.13694331251424074 1105 10-11 0.26296093595056647 1105 12-13 0.2807571691119435 1105 14-15 0.3112651289997572 1105 16-17 0.07960061280107311 1105 18-19 0.11678863068513579 1105 20-21 0.1532968404575641 1105 22-23 0.17396750735525757 1105 24-25 0.2773560303957723 1105 26-27 0.2738647102282883 1105 28-29 0.26971955516453505 1105 30-31 0.4862773023696718 1105 32-33 0.5183625075718297 1105 34-35 0.5131574587908716 1105 36-37 0.4650969960639273 1105 38-39 0.44538813407902467 1105 40-41 0.28865865626946885 1105 42-43 0.3447179379612848 1105 44-45 0.5813863744804948 1105 46-47 0.4730077449907597 1105 48-49 0.6621682102327853 1105 50-51 0.6104701173852547 1105 52-53 0.678844920024595 1105 54-55 0.6797342471041006 1105 56-57 0.7003420571485606 1105 58-59 0.6509656888380704 1105 60-61 0.8226283820783351 1105 62-63 0.7221156784085281 1105 64-65 0.8622735495198341 1105 66-67 0.8660740057985841 1105 68-69 0.6579254964910284 1105 70-71 0.5081834769592533 1105 72-73 0.6057999315661213 1105 74-75 0.7425708714595736 1105 76-77 0.5822435582271197 1105 78-79 0.7172774228972649 1105 80-81 0.8510023669859343 1105 82-83 0.9291623229166213 1105 84-85 1.0717994033943654 1105 86-87 1.084661883651318 1105 88-89 1.090054000935453 1105 90-91 1.2781772799680091 1105 92-93 1.2684286845944648 1105 94-95 0.9836501607613357 1105 96-97 1.1779560697624447 1105 98-99 1.3814367359275685 1105 100-101 1.6113078707320554 1106 1 0.38229828219584405 1106 2 0.2638380777612106 1106 3 0.30014881605761445 1106 4 0.28205583861390693 1106 5 0.15397943001030967 1106 6 0.28817718498943634 1106 7 0.21826471192431995 1106 8 0.23975639441177776 1106 9 0.3142615430271647 1106 10-11 0.4071492737912692 1106 12-13 0.41268184844648914 1106 14-15 0.5784090007882057 1106 16-17 0.497576298854959 1106 18-19 0.5673700110102686 1106 20-21 0.5216896333940255 1106 22-23 0.4942187682000849 1106 24-25 0.4472463326515239 1106 26-27 0.5396550795962014 1106 28-29 0.576930642477528 1106 30-31 0.6920279434052503 1106 32-33 0.7429315020485561 1106 34-35 0.6600291756292336 1106 36-37 0.5970043251697064 1106 38-39 0.6973889476940016 1106 40-41 0.6432137188816469 1106 42-43 0.6815602627738073 1106 44-45 0.8486768003289384 1106 46-47 0.5998814774088927 1106 48-49 0.4385187778805886 1106 50-51 0.4203014583955067 1106 52-53 0.571624013220422 1106 54-55 0.7662681439137984 1106 56-57 0.7903554131331703 1106 58-59 0.6295573523115578 1106 60-61 0.788260532126067 1106 62-63 0.8964053711175168 1106 64-65 0.8922434856340686 1106 66-67 1.021137060959763 1106 68-69 1.1785281548413984 1106 70-71 1.0838051873374184 1106 72-73 1.0212768155241 1106 74-75 0.9354948944386585 1106 76-77 0.8511340812368466 1106 78-79 1.1026922668670274 1106 80-81 0.894790283695972 1106 82-83 1.1809800362450673 1106 84-85 1.0477366664437682 1106 86-87 0.7554368629971648 1106 88-89 1.1121829256864046 1106 90-91 1.2833144242975472 1106 92-93 1.268848412469044 1106 94-95 1.1646112010072223 1106 96-97 1.4255194700824276 1106 98-99 1.599638381024576 1106 100-101 1.8859872791114043 1107 1 0.23405022714760904 1107 2 0.11846918508259918 1107 3 0.13245220356728993 1107 4 0.16567791398450993 1107 5 0.11081888098753723 1107 6 0.1048687216885753 1107 7 0.1767807295695576 1107 8 0.16254560216201241 1107 9 0.06557850246884556 1107 10-11 0.15935310435240524 1107 12-13 0.16057000819851197 1107 14-15 0.28469545363021354 1107 16-17 0.23349822807811194 1107 18-19 0.30073464793660776 1107 20-21 0.2822601653822616 1107 22-23 0.24343723463130118 1107 24-25 0.26797103963644275 1107 26-27 0.3218504409030345 1107 28-29 0.3026452958238437 1107 30-31 0.28722831520743597 1107 32-33 0.2627764436375841 1107 34-35 0.2089161696585009 1107 36-37 0.25923106688818365 1107 38-39 0.33226422304079506 1107 40-41 0.31427748410853695 1107 42-43 0.3736260502512181 1107 44-45 0.5522544361202719 1107 46-47 0.4217066113133683 1107 48-49 0.43321579558920575 1107 50-51 0.34858915185844097 1107 52-53 0.5311592220806887 1107 54-55 0.41392392730061545 1107 56-57 0.5448576571974968 1107 58-59 0.5011582718305974 1107 60-61 0.4621487264464861 1107 62-63 0.346979878470016 1107 64-65 0.5164859098764936 1107 66-67 0.291419580687748 1107 68-69 0.5905510391122029 1107 70-71 0.4740536380016138 1107 72-73 0.5041090032995683 1107 74-75 0.45620558202304196 1107 76-77 0.46795505733280507 1107 78-79 0.5852116107590781 1107 80-81 0.6474978524438519 1107 82-83 0.7723830709651693 1107 84-85 0.604907973916113 1107 86-87 0.7340649590281352 1107 88-89 0.64165401019266 1107 90-91 0.8917080122462444 1107 92-93 0.8381182705658254 1107 94-95 0.6442081720485184 1107 96-97 0.5159142602427309 1107 98-99 0.5705777000658045 1107 100-101 0.6167777236416256 1108 1 0.013369293359790646 1108 2 -0.263425038088851 1108 3 -0.10165664136454922 1108 4 0.04743460149091305 1108 5 0.04287262569356898 1108 6 0.06755721332568498 1108 7 -0.014641805781401729 1108 8 0.02937305771914822 1108 9 -0.0056722455466413635 1108 10-11 -0.05834684307987459 1108 12-13 -0.058025243514897795 1108 14-15 -0.10418019230802855 1108 16-17 0.011393171989347195 1108 18-19 -0.006097502347557793 1108 20-21 -0.07838297915912307 1108 22-23 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>>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 9.0 5 51.0 6 113.0 7 319.0 8 657.0 9 1404.0 10 2396.0 11 4024.0 12 6241.0 13 8451.0 14 10573.0 15 15211.0 16 20237.0 17 28577.0 18 23877.0 19 12276.0 20 9670.0 21 9508.0 22 9753.0 23 10761.0 24 12184.0 25 14546.0 26 17214.0 27 21213.0 28 26056.0 29 32599.0 30 39755.0 31 48944.0 32 61375.0 33 78739.0 34 104858.0 35 146448.0 36 212794.0 37 310125.0 38 236439.0 39 19163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6309035323206 19.925220745942017 15.43261835336571 20.01125736837168 2 15.186626183559113 22.483286584167132 40.126203475156984 22.203883757116774 3 18.995827888515837 28.13226377201537 31.480232260816717 21.391676078652075 4 12.158798889859689 18.014981754638434 38.47092306110911 31.355296294392765 5 12.149663001907438 39.79185846529828 33.2708441880919 14.787634344702374 6 28.25271335160437 38.278141185510115 18.071943167056627 15.397202295828885 7 25.72774024856013 36.40310555614446 20.85174727685641 17.017406918438997 8 26.476568598163265 33.42468833617613 20.206930002923105 19.8918130627375 9 27.81098123110824 16.329867368211413 20.938669442008525 34.920481958671814 10-11 24.066445088812632 27.901270972883534 27.04557186391689 20.986712074386944 12-13 26.68197395982735 25.97535007563169 27.841649178812332 19.501026785728627 14-15 23.45240963274903 25.89650930092865 28.653766466546532 21.997314599775788 16-17 20.01538644386839 29.496230481862305 28.92002582609785 21.568357248171456 18-19 19.951303029426974 27.250901825493955 33.391088711361235 19.406706433717833 20-21 21.829067368314877 25.733083075092573 33.006526394845444 19.43132316174711 22-23 21.037059165534366 26.037589178736514 32.831940844102235 20.09341081162688 24-25 20.738549941391298 26.139898278809092 33.441620497658384 19.679931282141226 26-27 20.808214878947975 26.008493830713796 33.47189907882315 19.71139221151508 28-29 20.19329022219124 26.256914866790936 33.69874261131967 19.85105229969814 30-31 20.29905592062357 26.517848709561143 33.51224771594217 19.670847653873118 32-33 20.14778138402987 26.594545907760462 33.016158750472194 20.241513957737467 34-35 20.719125002690276 26.249758678034258 32.8995801881171 20.131536131158366 36-37 21.06528982220435 26.05756097310258 32.95544636512805 19.921702839565025 38-39 21.105749107709883 26.11985582017933 32.95445602184605 19.81993905026474 40-41 20.5655439458298 26.319602004880814 33.085223496411665 20.02963055287772 42-43 20.423223588117025 26.808036725291746 32.33218515935612 20.43655452723511 44-45 20.77068170327203 27.111952854922755 31.599999229067745 20.51736621273747 46-47 21.017163771346304 27.088294181380974 30.5626464965933 21.33189555067943 48-49 21.39915448249234 27.365960162833268 30.10664853267924 21.12823682199515 50-51 21.604146322660224 27.765778383101196 29.811571670863955 20.81850362337462 52-53 21.341868470318257 28.1469998316786 29.668862982526694 20.842268715476443 54-55 20.798783694230032 27.825210975919624 29.87639385769441 21.499611472155934 56-57 21.354745534698996 28.466751640478545 28.95712848477217 21.22137434005029 58-59 21.429590168989762 27.785080280416103 29.26933000287817 21.515999547715968 60-61 21.431824971330734 27.57009916192593 29.79390252128914 21.204173345454194 62-63 22.032552447215505 27.451953500175065 29.527196101672594 20.988297950936836 64-65 21.59665930536037 27.67482834772134 29.78283878739209 20.9456735595262 66-67 21.250833166030915 27.99829753061634 29.523710098373822 21.22715920497892 68-69 21.32969754867209 28.17779154027989 29.37186348525872 21.120647425789294 70-71 21.3367481261576 28.19272742924948 28.988969915088454 21.481554529504464 72-73 21.371738411181028 28.05844921349253 28.78263997969889 21.78717239562755 74-75 21.290714471334642 28.335527814120464 28.81851702781107 21.555240686733825 76-77 21.503377168369518 28.04323392259383 29.006386216231927 21.447002692804723 78-79 21.184810617765702 28.134907658614082 29.03590029558752 21.644381428032695 80-81 20.890537914197143 28.611188743796077 28.738013781140076 21.7602595608667 82-83 21.05023590845313 28.472614831908803 28.483536519553677 21.99361274008439 84-85 21.06553156276913 28.006831953161864 28.502299536237974 22.425336947831028 86-87 21.20146427896853 27.951172284061897 28.643222698636844 22.204140738332732 88-89 21.518670183855793 27.573004588364235 28.621511988723753 22.28681323905622 90-91 20.819055071368826 27.84023379730457 29.16125409717056 22.179457034156044 92-93 20.680196895212788 28.13173759639822 28.84147391305465 22.34659159533434 94-95 20.542754186954447 28.12668440234594 28.588311026679968 22.742250384019645 96-97 20.704101476546978 27.904283675877927 28.20559357085633 23.18602127671877 98-99 20.74737900810109 27.772796935727435 28.28245968007468 23.1973643760968 100-101 20.678534178066638 27.967330240375087 28.437312987437252 22.916822594121022 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2085.0 1 1454.0 2 925.0 3 1007.0 4 846.5 5 848.0 6 1448.5 7 3009.0 8 4921.0 9 5558.0 10 4661.0 11 3847.0 12 3928.0 13 4170.5 14 4308.5 15 4486.5 16 4713.5 17 4875.0 18 5036.0 19 5247.0 20 5515.0 21 6060.0 22 6793.5 23 7753.0 24 8912.0 25 10442.5 26 12724.5 27 15447.5 28 17293.5 29 18861.0 30 21054.5 31 23933.5 32 27701.5 33 31557.5 34 36175.5 35 39156.0 36 41246.5 37 44668.0 38 45882.0 39 44865.5 40 44911.0 41 46433.5 42 48310.5 43 50248.5 44 52493.5 45 53476.0 46 53333.5 47 56746.5 48 76252.5 49 75852.5 50 55092.5 51 48375.5 52 43565.5 53 41093.5 54 39134.5 55 35800.5 56 32508.0 57 29224.5 58 27003.0 59 24977.0 60 21770.0 61 18065.5 62 14418.0 63 10500.0 64 7030.0 65 4964.5 66 3530.5 67 2674.5 68 1918.0 69 1221.5 70 892.5 71 693.5 72 521.0 73 400.0 74 281.5 75 190.5 76 139.5 77 68.5 78 30.0 79 13.5 80 8.5 81 10.5 82 7.5 83 4.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016125250150170405 2 0.0019915647595827994 3 0.0025055169556041667 4 3.2122012251335476E-4 5 0.0017988326860747864 6 1.9273207350801287E-4 7 0.0 8 0.0 9 0.0 10-11 9.636603675400643E-5 12-13 5.46074208272703E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0025376389678555024 22-23 0.0 24-25 0.003565543359898237 26-27 0.0015097345758127672 28-29 0.002923103114871528 30-31 0.015033101733625003 32-33 5.781962205240385E-4 34-35 0.002216418845342148 36-37 0.0037903974456575855 38-39 0.002344906894347489 40-41 0.004079495555919605 42-43 0.0022806628698448187 44-45 5.139521960213676E-4 46-47 9.636603675400643E-5 48-49 2.248540857593483E-4 50-51 3.2122012251335476E-4 52-53 0.0012206364655507481 54-55 0.0031158351883795407 56-57 4.497081715186966E-4 58-59 0.0013491245145560897 60-61 0.001284880490053419 62-63 0.0 64-65 1.6061006125667738E-4 66-67 1.6061006125667738E-4 68-69 3.212201225133547E-5 70-71 2.569760980106838E-4 72-73 0.0 74-75 5.46074208272703E-4 76-77 6.103182327753741E-4 78-79 2.569760980106838E-4 80-81 0.005781962205240385 82-83 0.0018630767105774575 84-85 0.003565543359898237 86-87 0.0019915647595827994 88-89 0.0012206364655507481 90-91 1.9273207350801287E-4 92-93 6.424402450267095E-5 94-95 2.569760980106838E-4 96-97 0.0017988326860747864 98-99 0.002923103114871528 100-101 0.0011885144532994124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1556565.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.18039609320005 #Duplication Level Percentage of deduplicated Percentage of total 1 68.47638403459685 30.253137696785693 2 15.538810969757927 13.730216469225345 3 6.332016134078443 8.392529426163568 4 3.212676082264761 5.6774920733442915 5 1.8259445641280143 4.03354770437006 6 1.105577380406972 2.930690794683752 7 0.7226954750368876 2.235028063932515 8 0.5006311611125586 1.7694466395641189 9 0.3512258053944432 1.3965565680431729 >10 1.73719828972292 13.677784776737292 >50 0.12210468213405361 3.7104990550645556 >100 0.06761092122041122 5.64329740267717 >500 0.003634949053933753 1.1292934145171767 >1k 0.002762561280989652 2.253704786969319 >5k 4.361938864720503E-4 1.3285578156452928 >10k+ 2.9079592431470024E-4 1.838217312276651 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16400 1.0536020018438035 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12216 0.7848050033246283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8829 0.5672104923340817 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6710 0.431077404412922 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5143 0.33040701801723665 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3348 0.21508899403494233 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2518 0.16176645369772544 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2431 0.15617722356599306 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2427 0.1559202474679824 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2350 0.15097345758127673 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2195 0.14101563378336274 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2158 0.1386386048767639 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2059 0.13227844645099948 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1927 0.1237982352166469 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1904 0.12232062265308548 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1751 0.11249128690417683 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1659 0.10658083664993111 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.424402450267095E-5 0.0 0.0 0.0 0.0 6 1.284880490053419E-4 0.0 0.0 0.0 0.0 7 3.212201225133547E-4 0.0 0.0 0.0 0.0 8 3.212201225133547E-4 0.0 0.0 0.0 0.0 9 3.212201225133547E-4 0.0 0.0 0.0 0.0 10-11 3.854641470160257E-4 0.0 0.0 0.0 0.0 12-13 4.4970817151869665E-4 0.0 0.0 3.212201225133547E-5 0.0 14-15 5.139521960213676E-4 0.0 0.0 6.424402450267095E-5 0.0 16-17 5.139521960213676E-4 0.0 0.0 1.6061006125667735E-4 0.0 18-19 5.46074208272703E-4 0.0 0.0 1.9273207350801284E-4 0.0 20-21 5.781962205240385E-4 0.0 0.0 2.569760980106838E-4 0.0 22-23 6.424402450267094E-4 0.0 0.0 8.994163430373933E-4 0.0 24-25 7.066842695293804E-4 0.0 0.0 0.0016061006125667736 0.0 26-27 7.066842695293804E-4 0.0 0.0 0.0025697609801068376 0.0 28-29 9.636603675400642E-4 0.0 0.0 0.004625569764192308 0.0 30-31 0.0010279043920427351 0.0 0.0 0.008705065320111912 0.0 32-33 0.0010279043920427351 0.0 0.0 0.013266391059801552 0.0 34-35 0.0011242704287967415 0.0 0.0 0.01837379100776389 0.0 36-37 0.00134912451455609 0.0 0.0 0.02489455949478499 0.0 38-39 0.0015418565880641027 0.0 0.0 0.03453116317018563 0.0 40-41 0.0015418565880641027 0.0 0.0 0.053065564239206206 0.0 42-43 0.0015739786003154382 0.0 0.0 0.07224240555325348 0.0 44-45 0.0016061006125667736 0.0 0.0 0.09305746949211886 0.0 46-47 0.001895198722828793 0.0 0.0 0.13083295589968938 0.0 48-49 0.002120052808588141 0.0 0.0 0.15636995563950107 0.0 50-51 0.002120052808588141 0.0 0.0 0.19218599929974012 0.0 52-53 0.002152174820839477 0.0 0.0 0.23288458882218216 0.0 54-55 0.002184296833090812 0.0 0.0 0.27910816445185394 0.0 56-57 0.002248540857593483 0.0 0.0 0.38148101749686003 0.0 58-59 0.002248540857593483 0.0 0.0 0.4890576365265826 0.0 60-61 0.002248540857593483 0.0 0.0 0.5830145223617389 0.0 62-63 0.002312784882096154 0.0 0.0 0.6883426005338679 0.0 64-65 0.002312784882096154 0.0 0.0 0.7857686636921684 0.0 66-67 0.002441272931101496 0.0 0.0 0.9277479578430712 0.0 68-69 0.0025697609801068376 0.0 0.0 1.1084985207813358 0.0 70-71 0.0025697609801068376 0.0 0.0 1.214019331026973 0.0 72-73 0.002634005004609509 0.0 0.0 1.325097249392091 0.0 74-75 0.00269824902911218 0.0 0.0 1.5181505430226172 0.0 76-77 0.00269824902911218 0.0 0.0 1.727650306925827 0.0 78-79 0.0027303710413635156 0.0 0.0 1.9300832281337432 0.0 80-81 0.0030194691516255345 0.0 0.0 2.115748458946462 0.0 82-83 0.0030837131761282054 0.0 0.0 2.3693838676830072 0.0 84-85 0.0031479572006308763 0.0 0.0 2.7327159482578627 0.0 86-87 0.0032122012251335473 0.0 0.0 3.1333095630442673 0.0 88-89 0.003340689274138889 0.0 0.0 3.591754921895327 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGCG 85 6.4108684E-4 27.939194 5 GTATCAA 23090 0.0 24.29889 1 GGTATCA 17290 0.0 24.20702 1 GAGTACT 12885 0.0 23.240715 12-13 GTACATG 13630 0.0 22.655724 1 AGTACTT 12935 0.0 22.545029 12-13 TACATGG 13815 0.0 22.176119 2 GTACTTT 13915 0.0 21.55663 14-15 ACATGGG 13840 0.0 21.00352 3 TATCAAC 27780 0.0 20.1927 2 ATCAACG 27860 0.0 20.168814 3 TCAACGC 27985 0.0 20.061108 4 CAACGCA 28185 0.0 19.901903 5 AACGCAG 28680 0.0 19.57434 6 ACTTTTT 15550 0.0 19.550335 16-17 TACTTTT 15095 0.0 19.399506 14-15 CATGGGA 9855 0.0 19.326382 4 ATAGCGT 150 7.728097E-4 18.99804 6 ATGGGAG 3645 0.0 18.633793 5 GGACCGC 180 1.2346526E-4 18.470318 6 >>END_MODULE