FastQCFastQC Report
Thu 26 May 2016
SRR937110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330962
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135451.0176849526883562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93890.7054296065552584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63920.4802541319737152No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA55530.41721702047090753No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA42960.3227740536544244No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT35580.26732543829200234No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT21040.1580811473205095No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20300.15252125905923686No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.14485762929369886No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA18910.14207768516306252No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17100.12847849901049013No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG15480.11630685173581214No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC15470.1162317181106598No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.1128507049788048No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14310.10751621759298913No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.10188119570656412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA193450.025.3853021
GTACATG111300.025.1149831
GAGTACT91650.024.84584412-13
TACATGG113350.024.5349432
ACATGGG113950.023.31933
AGTACTT93500.023.08314312-13
GTACTTT102250.022.31579214-15
CATGGGA80650.021.8669194
TATCAAC224200.021.7127632
ATCAACG225200.021.6358283
GCGCTAT550.002889441321.57660340-41
GGAGTCG1553.880063E-521.4708778
GGTATCA142550.021.3433931
TCAACGC227850.021.3216064
CAACGCA228800.021.195515
AACGCAG231300.021.0272966
ACCGCCG704.8529822E-420.35780586-87
ACTTTTT115950.020.02455316-17
ATGGGAT31000.019.9379925
ACGCAGA246300.019.7274027
Produced by FastQC (version 0.11.5)