##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937110_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1330962 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807016278451226 33.0 31.0 34.0 30.0 34.0 2 31.883931321855922 33.0 31.0 34.0 30.0 34.0 3 31.873623739821273 33.0 31.0 34.0 30.0 34.0 4 35.49047155365818 37.0 35.0 37.0 33.0 37.0 5 35.509683972945886 37.0 35.0 37.0 33.0 37.0 6 35.59958586345816 37.0 35.0 37.0 33.0 37.0 7 35.54649268724427 37.0 35.0 37.0 33.0 37.0 8 35.50542089105474 37.0 35.0 37.0 33.0 37.0 9 37.12509222652488 39.0 37.0 39.0 33.0 39.0 10-11 36.954843564279074 39.0 37.0 39.0 33.0 39.0 12-13 36.89135527535722 39.0 37.0 39.0 33.0 39.0 14-15 37.96257180896224 40.0 38.0 41.0 33.0 41.0 16-17 37.927708679887175 40.0 38.0 41.0 33.0 41.0 18-19 37.99030588401472 40.0 38.0 41.0 33.0 41.0 20-21 37.963338923275046 40.0 38.0 41.0 33.0 41.0 22-23 37.85740915217715 40.0 37.5 41.0 33.0 41.0 24-25 37.706686592104056 40.0 37.0 41.0 32.0 41.0 26-27 37.73964846479464 40.0 37.0 41.0 32.5 41.0 28-29 37.666121572216184 40.0 37.0 41.0 32.5 41.0 30-31 37.45989141688493 40.0 37.0 41.0 32.0 41.0 32-33 37.18445492808961 39.5 36.0 41.0 31.5 41.0 34-35 36.980976541779555 39.0 35.0 41.0 31.0 41.0 36-37 36.75855396322359 39.0 35.0 41.0 31.0 41.0 38-39 36.55765115758376 39.0 35.0 41.0 30.0 41.0 40-41 36.32820546341669 39.0 35.0 41.0 30.0 41.0 42-43 36.042609030160136 39.0 35.0 41.0 30.0 41.0 44-45 35.659928683163 39.0 35.0 40.5 28.0 41.0 46-47 35.100511134051914 38.5 35.0 40.0 25.0 41.0 48-49 34.619565772726794 38.0 34.5 40.0 23.5 41.0 50-51 33.877782010305324 38.0 33.5 39.5 22.5 40.5 52-53 34.0624187617678 38.0 34.0 40.0 22.0 40.5 54-55 34.436375343548505 39.0 35.0 40.0 22.0 41.0 56-57 34.34703319854361 39.0 34.0 41.0 21.0 41.0 58-59 34.12331757029878 38.0 34.0 40.0 20.0 41.0 60-61 33.848423170608925 38.0 34.0 40.0 18.5 41.0 62-63 33.53251069527154 37.5 34.0 40.0 17.5 41.0 64-65 33.19649809686527 37.0 33.0 40.0 13.5 41.0 66-67 32.829210375653105 36.5 33.0 39.5 10.0 41.0 68-69 32.46133811483723 36.0 32.5 39.0 8.0 41.0 70-71 32.05181853426319 35.5 32.0 39.0 7.0 40.5 72-73 31.658134492194367 35.0 32.0 38.0 2.0 40.0 74-75 31.228106437298734 35.0 31.0 37.0 2.0 39.5 76-77 30.795212034603544 35.0 31.0 37.0 2.0 39.0 78-79 30.358357338526567 35.0 31.0 36.0 2.0 39.0 80-81 29.955135458412787 35.0 30.0 36.0 2.0 37.5 82-83 29.565762208087083 34.0 30.0 35.5 2.0 37.0 84-85 29.22318405784688 34.0 29.5 35.0 2.0 37.0 86-87 28.891618243045258 34.0 29.0 35.0 2.0 36.0 88-89 28.524429322550155 34.0 29.0 35.0 2.0 36.0 90-91 28.163509551737768 34.0 29.0 35.0 2.0 36.0 92-93 27.83204742133885 34.0 28.5 35.0 2.0 35.0 94-95 27.457469484478146 34.0 27.0 35.0 2.0 35.0 96-97 27.01429342084898 34.0 26.5 35.0 2.0 35.0 98-99 26.451863013369277 34.0 25.0 35.0 2.0 35.0 100-101 24.443626489711953 31.0 13.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34389210060394504 1101 2 0.3581715599155473 1101 3 0.3112430751940245 1101 4 0.2884194750011275 1101 5 0.24805157608017936 1101 6 0.2432068327077701 1101 7 0.3113024790498926 1101 8 0.1545924966757397 1101 9 0.2339387800810684 1101 10-11 0.3827028988981809 1101 12-13 0.3959017654847301 1101 14-15 0.5596565500197741 1101 16-17 0.7034278054201053 1101 18-19 0.5369316993933637 1101 20-21 0.5442334119641572 1101 22-23 0.5670789736000543 1101 24-25 0.55331024576342 1101 26-27 0.567228251712983 1101 28-29 0.6365171061228168 1101 30-31 0.5819998956527641 1101 32-33 0.6474718245767264 1101 34-35 0.5786790961860717 1101 36-37 0.5999041454341167 1101 38-39 0.5729813273507531 1101 40-41 0.6683980844087429 1101 42-43 0.6726732422765238 1101 44-45 0.588508938083983 1101 46-47 0.6774982858789897 1101 48-49 0.9842156873198249 1101 50-51 0.8184679184172197 1101 52-53 0.8106903547058124 1101 54-55 0.9686683683481405 1101 56-57 0.8703523818030803 1101 58-59 0.7612882713495068 1101 60-61 0.8093994244077081 1101 62-63 0.9014698438515367 1101 64-65 1.0097518972057173 1101 66-67 1.0097234526014915 1101 68-69 0.9600726961512152 1101 70-71 0.94869042071171 1101 72-73 0.9521083541808544 1101 74-75 0.8849360303358758 1101 76-77 0.8376987878577928 1101 78-79 0.8427614397224019 1101 80-81 0.8545518177405498 1101 82-83 1.0085479713807288 1101 84-85 1.0386704219229728 1101 86-87 0.9402414979151317 1101 88-89 0.8708116284370178 1101 90-91 0.9881097077481193 1101 92-93 1.109438124032227 1101 94-95 1.1416941541497678 1101 96-97 1.0835018239563077 1101 98-99 1.0547981014491512 1101 100-101 1.1282433639970826 1102 1 0.24011953368732009 1102 2 0.24608297271819168 1102 3 0.1525570783362724 1102 4 0.22250206545582785 1102 5 0.06690908725932587 1102 6 0.061005418121069965 1102 7 0.11725972779961324 1102 8 0.07964292513781857 1102 9 0.13296243901489646 1102 10-11 0.21335984291899734 1102 12-13 0.3026731353548371 1102 14-15 0.30379972656687215 1102 16-17 0.2942022743745909 1102 18-19 0.2590250129102074 1102 20-21 0.27547075089521655 1102 22-23 0.21778543966551211 1102 24-25 0.3398144095285929 1102 26-27 0.3331488265255089 1102 28-29 0.33613180677485843 1102 30-31 0.3925665648132721 1102 32-33 0.35345446740222286 1102 34-35 0.27347539685192856 1102 36-37 0.1129723828406668 1102 38-39 0.23806224309134194 1102 40-41 0.22719939770689734 1102 42-43 0.18594092748782742 1102 44-45 0.0514107471780747 1102 46-47 0.06430653111238627 1102 48-49 0.3442855174312953 1102 50-51 0.07200456501801256 1102 52-53 -0.01821274351530633 1102 54-55 0.08603395959129045 1102 56-57 0.13208796976585546 1102 58-59 0.24495297459132104 1102 60-61 -0.00993413576977531 1102 62-63 0.18534377845136163 1102 64-65 0.36886099677351325 1102 66-67 0.02794568025902322 1102 68-69 0.09499167250379514 1102 70-71 0.3045544019848343 1102 72-73 0.10415862006329846 1102 74-75 0.11806929240273689 1102 76-77 0.05878910731958342 1102 78-79 0.14492971971618474 1102 80-81 0.1427324940718293 1102 82-83 -0.07960813816054113 1102 84-85 -0.03703161996214632 1102 86-87 -0.10806317776007646 1102 88-89 -0.1044683538374862 1102 90-91 -0.07169316084724997 1102 92-93 -0.06608331777577447 1102 94-95 -0.025810349162252777 1102 96-97 -0.09012333502643344 1102 98-99 -0.002846630395165306 1102 100-101 0.2383207475871849 1103 1 0.36977543658993994 1103 2 0.3069704730825933 1103 3 0.2703916279781815 1103 4 0.24338013983366835 1103 5 0.12277020457884902 1103 6 0.09226665399423695 1103 7 0.24117986928659008 1103 8 0.269265594149644 1103 9 0.2529469881709261 1103 10-11 0.31847416944682294 1103 12-13 0.2025341749342644 1103 14-15 0.4082374713267001 1103 16-17 0.3618555924190332 1103 18-19 0.2812538214460716 1103 20-21 0.5336699015846733 1103 22-23 0.3911798637571664 1103 24-25 0.35260994336010754 1103 26-27 0.5144071471450999 1103 28-29 0.48253584148581297 1103 30-31 0.48782854893486416 1103 32-33 0.6713798757806515 1103 34-35 0.7519328875960056 1103 36-37 0.3460274714157876 1103 38-39 0.36931667670615553 1103 40-41 0.46649555831793776 1103 42-43 0.5325026288298105 1103 44-45 0.6384687218542453 1103 46-47 0.6865917012174307 1103 48-49 0.8657575747257482 1103 50-51 0.781867596687384 1103 52-53 0.7539577599489817 1103 54-55 0.8560046612928218 1103 56-57 0.9013359315177283 1103 58-59 0.9050392660972406 1103 60-61 0.8321514366402667 1103 62-63 0.8516322719454834 1103 64-65 0.8455851263273857 1103 66-67 0.8974832238663595 1103 68-69 0.8963754462023701 1103 70-71 0.7339250959123653 1103 72-73 0.627332456634182 1103 74-75 0.6580362042746231 1103 76-77 0.737405479383682 1103 78-79 0.7701690134474184 1103 80-81 0.731880948501562 1103 82-83 0.7537341028920643 1103 84-85 0.6998266598438931 1103 86-87 0.6823240165027045 1103 88-89 0.6509212212157145 1103 90-91 0.8195703100536598 1103 92-93 0.9559705755453791 1103 94-95 0.9857717103672243 1103 96-97 0.9208683856534066 1103 98-99 1.0218115561227599 1103 100-101 1.1885257870888708 1104 1 0.21463510323702195 1104 2 0.1536341458964543 1104 3 0.1924843031115735 1104 4 0.06830690235958059 1104 5 0.09986862958842835 1104 6 0.06305000817261686 1104 7 0.13951828097772534 1104 8 0.062023827944372556 1104 9 0.06494352425524852 1104 10-11 0.2793848043394789 1104 12-13 0.23031964719356068 1104 14-15 0.28411674071469406 1104 16-17 0.32713809181046827 1104 18-19 0.37401709162869423 1104 20-21 0.3345947782631171 1104 22-23 0.2740649585671662 1104 24-25 0.34446218693101827 1104 26-27 0.05223741527679948 1104 28-29 0.1846055171144556 1104 30-31 0.20702019468652821 1104 32-33 0.37476880524044276 1104 34-35 0.2762582914163829 1104 36-37 0.2189985765391924 1104 38-39 0.20788435596659127 1104 40-41 0.15394082257441255 1104 42-43 0.06450222871677624 1104 44-45 0.17210724630450613 1104 46-47 0.3624029094189112 1104 48-49 0.31832074641901187 1104 50-51 0.1529825830191882 1104 52-53 0.11938827318488876 1104 54-55 0.10048666879703916 1104 56-57 0.14834837419384428 1104 58-59 0.2734118692662477 1104 60-61 0.16773645506504664 1104 62-63 0.11349506395105635 1104 64-65 0.21965514331120062 1104 66-67 0.019875144380961274 1104 68-69 0.108562665528666 1104 70-71 0.07108888204495045 1104 72-73 0.1616897156080519 1104 74-75 0.38833938083639197 1104 76-77 0.09167304868297776 1104 78-79 0.1544929763012597 1104 80-81 0.22100374962858993 1104 82-83 0.11479275162918867 1104 84-85 0.2091366327313402 1104 86-87 0.23833389621697165 1104 88-89 -0.03751068542084468 1104 90-91 0.001495833068553054 1104 92-93 0.11327935368969477 1104 94-95 0.10313178971831505 1104 96-97 0.1563191703506206 1104 98-99 0.19836773003482833 1104 100-101 0.14126628160620314 1105 1 0.10199459206735284 1105 2 0.2644879111985077 1105 3 0.4148554208227644 1105 4 0.23585835183569515 1105 5 0.20227973432852764 1105 6 0.2866867375400375 1105 7 0.25053597448638243 1105 8 0.24045323823948905 1105 9 0.2685781490421917 1105 10-11 0.2412883186309429 1105 12-13 0.2766716003372949 1105 14-15 0.31640111248668745 1105 16-17 0.3337202746040191 1105 18-19 0.31891538097701044 1105 20-21 0.28770862676051934 1105 22-23 0.2700882678696317 1105 24-25 0.28241231839572123 1105 26-27 0.18147243606848207 1105 28-29 0.15074718401376686 1105 30-31 -0.07364458072108704 1105 32-33 0.018557644235031034 1105 34-35 6.68232710694383E-5 1105 36-37 -0.07455210125741019 1105 38-39 -0.11476626291607772 1105 40-41 -0.03234926735703425 1105 42-43 -0.04919254125758954 1105 44-45 -0.06882122444576311 1105 46-47 -0.018735360802061507 1105 48-49 0.15032457904616336 1105 50-51 0.0025723687886411994 1105 52-53 -0.17345568792188715 1105 54-55 -0.08281477369959589 1105 56-57 0.061707664019628794 1105 58-59 -0.08737299607997073 1105 60-61 -0.10996776977287226 1105 62-63 -0.08446059382698223 1105 64-65 -0.10960933444861354 1105 66-67 -0.06424132195684251 1105 68-69 -0.07132527629900665 1105 70-71 -0.08855446314991511 1105 72-73 -0.04203546340656317 1105 74-75 0.004564121396740006 1105 76-77 -0.010581823170262794 1105 78-79 -0.08908143171389682 1105 80-81 -0.03710944387183446 1105 82-83 -0.043281106260089075 1105 84-85 -0.16985911730463243 1105 86-87 -0.18979629141281862 1105 88-89 -0.052960382010493134 1105 90-91 -0.06999565590781387 1105 92-93 -0.07541382353244686 1105 94-95 -0.06875248269457401 1105 96-97 0.0056874024958659675 1105 98-99 0.22550250121181747 1105 100-101 0.3899340964861615 1106 1 0.14453240919225863 1106 2 0.08302048782041638 1106 3 0.07170527373376245 1106 4 0.04620789503626099 1106 5 -0.0679261977797907 1106 6 -0.056520811965796725 1106 7 0.04349033431249438 1106 8 0.06646951426332492 1106 9 0.039482852401135915 1106 10-11 0.09192160235308222 1106 12-13 0.037499178109129616 1106 14-15 0.20778129544657276 1106 16-17 0.22674937012577345 1106 18-19 0.1242630351503351 1106 20-21 0.15219277600230896 1106 22-23 0.040557575772815824 1106 24-25 0.17073460332705537 1106 26-27 0.05936079999829502 1106 28-29 0.0032985713701521036 1106 30-31 0.03656655887446192 1106 32-33 0.1927068222361683 1106 34-35 0.12219515481542231 1106 36-37 0.03946466850224084 1106 38-39 0.1135450967971039 1106 40-41 0.03625787612987352 1106 42-43 -0.051810354090449096 1106 44-45 -0.1501144896415454 1106 46-47 -0.07939076884132845 1106 48-49 0.10251718253840636 1106 50-51 0.18790207438927808 1106 52-53 0.09919341110403224 1106 54-55 0.17711556302748477 1106 56-57 -0.08132696539524176 1106 58-59 0.2670192079965048 1106 60-61 0.21593895392604168 1106 62-63 0.15371103789862417 1106 64-65 0.22870137620456177 1106 66-67 0.5011450957309052 1106 68-69 0.5255396137941233 1106 70-71 0.24507194766455243 1106 72-73 0.4379158983626006 1106 74-75 0.39565185217415433 1106 76-77 0.6258520910780305 1106 78-79 0.46877251327657987 1106 80-81 0.4562697688746553 1106 82-83 0.45586404794418556 1106 84-85 0.38911398722441604 1106 86-87 0.5033564631715919 1106 88-89 0.5087652246824028 1106 90-91 0.49760281831343534 1106 92-93 0.5028815723059914 1106 94-95 0.4786654778088142 1106 96-97 0.4471594480816421 1106 98-99 0.2749075983213203 1106 100-101 0.39750258638614966 1107 1 0.12431810550368283 1107 2 0.11700242825780194 1107 3 0.17497405846838276 1107 4 0.08418135383849545 1107 5 0.09884249039527049 1107 6 -0.03424227959298776 1107 7 0.030721845108629964 1107 8 -0.004878437601249175 1107 9 0.10167461772435615 1107 10-11 0.20969889895867055 1107 12-13 0.15990684669333177 1107 14-15 0.15027257094951807 1107 16-17 0.20290267158469533 1107 18-19 0.21089334080522093 1107 20-21 0.3948494707005068 1107 22-23 0.14665565296974137 1107 24-25 0.2592005158538555 1107 26-27 0.11016068373669441 1107 28-29 0.2276386014499181 1107 30-31 0.31753281765016084 1107 32-33 0.46341260430028086 1107 34-35 0.4322102337114089 1107 36-37 0.38253881258470557 1107 38-39 0.26193196703862753 1107 40-41 0.6643641189488676 1107 42-43 0.6024208075307413 1107 44-45 0.5862426207504043 1107 46-47 0.6290366369058162 1107 48-49 0.7926376771574084 1107 50-51 0.5916877941090775 1107 52-53 0.8473380046468364 1107 54-55 0.9927387037683957 1107 56-57 1.0300766203779261 1107 58-59 0.9068267131122028 1107 60-61 0.9566839645280822 1107 62-63 0.7798786769882682 1107 64-65 0.8944929597227329 1107 66-67 0.968979645324481 1107 68-69 1.0402043765794815 1107 70-71 0.9260811217975125 1107 72-73 1.0520703585361737 1107 74-75 1.0778792372439057 1107 76-77 1.1274645380921946 1107 78-79 1.0178858234746322 1107 80-81 0.9751378958338854 1107 82-83 0.9680085088395174 1107 84-85 1.0528089604705926 1107 86-87 0.9840160722126825 1107 88-89 0.9099407153317323 1107 90-91 1.1463517381143866 1107 92-93 1.3124575490692756 1107 94-95 1.1139274663113987 1107 96-97 0.9833091992701029 1107 98-99 1.0118091485410687 1107 100-101 0.7798749083215526 1108 1 0.04275634081550095 1108 2 0.15966209811595888 1108 3 0.07292536565595498 1108 4 0.14233751024765695 1108 5 0.09070188022061387 1108 6 -0.024752456860177574 1108 7 -0.018509769713851654 1108 8 0.06442446924371126 1108 9 -0.07159107444415014 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5 913.0 6 994.0 7 1302.0 8 1541.0 9 1976.0 10 2630.0 11 3646.0 12 5620.0 13 7519.0 14 8579.0 15 11335.0 16 15737.0 17 22157.0 18 18094.0 19 9297.0 20 8345.0 21 8126.0 22 8446.0 23 9607.0 24 10408.0 25 12134.0 26 13759.0 27 16110.0 28 19400.0 29 23586.0 30 28547.0 31 35413.0 32 44877.0 33 59049.0 34 81198.0 35 116475.0 36 172760.0 37 255198.0 38 245151.0 39 46872.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.686020134662016 19.124847231220905 14.824020889298446 20.36511174481864 2 14.903479372629075 21.571569126327287 41.07983743736099 22.445114063682645 3 18.744603286612545 28.184577555233414 31.165103921254765 21.90571523689928 4 11.754461262314933 17.36925143397046 39.25903320587764 31.617254097836963 5 12.273658490743351 39.20157879898506 33.56113147260596 14.963631237665634 6 28.048735562360307 37.82749938539241 18.73331443775247 15.390450614494815 7 25.912340437688847 36.14763446688765 21.0901548202685 16.849870275155006 8 26.06738903574855 33.114246790389664 20.553009319824252 20.265354854037536 9 27.907989080544894 16.39730156236021 21.131972486341283 34.56273687075362 10-11 23.843359779130477 27.561811286480765 27.40662518345644 21.18820375093232 12-13 26.499375949233844 25.799160914873458 27.877212372746275 19.82425076314642 14-15 23.456590098734477 25.68524884734594 28.583978813344284 22.274182240575303 16-17 20.12498524698982 29.446031045595632 28.70819260026597 21.720791107148578 18-19 19.933340424244452 27.4770425488493 32.910534625883656 19.679082401022594 20-21 22.016406844642404 26.035914639303133 32.282054896212145 19.66562361984232 22-23 21.11372152903351 26.358031093534628 32.02888845799457 20.49935891943729 24-25 20.709200778657774 26.392493264144356 32.749765827804815 20.14854012939305 26-27 20.859002658200502 26.330499798051903 32.67264683505067 20.13785070869692 28-29 20.18582712193773 26.556806559895037 32.98286759451228 20.274498723654954 30-31 20.347954286384127 26.841570565829603 32.80238982812684 20.008085319659425 32-33 20.157467949633098 26.991060010188793 32.25215159361442 20.599320446563688 34-35 20.683080300971316 26.56462215716648 32.22979438686318 20.522503154999026 36-37 21.01642293124189 26.371288836058465 32.245734598758865 20.366553633940775 38-39 21.04952912150645 26.443959128791196 32.30233603972158 20.204175709980777 40-41 20.636587414773285 26.645437318069042 32.41211335209812 20.305861915059566 42-43 20.356346133472385 27.081359413989215 31.78420268435499 20.778091768183412 44-45 20.63870239907855 27.38208878855944 31.110557621125086 20.868651191236925 46-47 20.820625986627324 27.265965048316094 30.19186684729319 21.721542117763388 48-49 21.180749260961047 27.521025371698364 29.81378359767429 21.484441769666287 50-51 21.446346939327547 27.99688026905425 29.482471800697883 21.07430099092032 52-53 21.154889405499144 28.34105243226754 29.31967739075421 21.184380771479105 54-55 20.603597873420345 27.987943220578725 29.66481687348008 21.743642032520842 56-57 21.147235824413002 28.65530695861026 28.704117029942854 21.49334018703388 58-59 21.217938915954115 27.920179646140387 29.132873748392463 21.72900768951304 60-61 21.26505883758662 27.69999481474746 29.57604172850769 21.458904619158233 62-63 21.83236416742468 27.536485085665703 29.333058307380576 21.298092439529047 64-65 21.40899986887887 27.846634113059476 29.625222746247548 21.119143271814103 66-67 20.997998877327348 28.18825131335417 29.339065927335877 21.474683881982607 68-69 21.045763078708227 28.31270159532735 29.255070507636947 21.38646481832748 70-71 21.00393586142762 28.357890979456496 28.930103234169664 21.708069924946223 72-73 21.117467722433595 28.19076547110105 28.652732386830316 22.03903441963504 74-75 21.027850920699045 28.466222999741497 28.765908585375826 21.740017494183636 76-77 21.325447753650664 28.151992932588666 28.856930388177794 21.66562892558288 78-79 20.94002977146772 28.255497969324338 28.99117867127256 21.813293587935377 80-81 20.757576497759754 28.707361003438063 28.68706857450572 21.847993924296464 82-83 20.809765526944336 28.611868103457343 28.438143996729192 22.14022237286913 84-85 20.961444597956483 28.107557288650938 28.417507987364505 22.51349012602808 86-87 21.183690002735517 28.04942569162 28.63511953610432 22.131764769540162 88-89 21.501032718987336 27.642300861081353 28.549861145606048 22.306805274325257 90-91 20.865333169013237 27.959039208377924 29.080110555361845 22.095517067247 92-93 20.86851923111611 28.341244836729416 28.725859643215745 22.064376288938725 94-95 20.657711311928157 28.391756063651908 28.521314370526234 22.4292182538937 96-97 20.77393702977143 28.14941336520177 28.202815546385864 22.87383405864093 98-99 20.896336959851457 28.041022441312613 28.235723051537402 22.826917547298528 100-101 21.321990955669246 28.075730293997136 28.358036711111446 22.244242039222172 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1167.0 1 826.0 2 593.0 3 712.0 4 685.0 5 717.0 6 1148.0 7 2355.0 8 3964.0 9 4564.5 10 3818.5 11 3273.5 12 3363.5 13 3530.0 14 3673.0 15 3744.0 16 3760.0 17 3819.5 18 3980.5 19 4145.0 20 4390.0 21 4878.0 22 5532.0 23 6412.5 24 7418.0 25 8758.5 26 10781.0 27 12962.5 28 14566.5 29 16092.5 30 18242.5 31 20666.5 32 23727.0 33 27218.0 34 31158.0 35 33609.5 36 35463.5 37 38670.0 38 39758.0 39 38892.0 40 38715.0 41 39857.0 42 41636.0 43 43158.0 44 44756.5 45 45708.5 46 45936.5 47 48826.5 48 65267.5 49 64872.0 50 47415.0 51 41686.5 52 37694.0 53 35709.0 54 33739.5 55 30823.0 56 28065.0 57 24982.5 58 22717.5 59 20830.5 60 18454.0 61 15544.5 62 12307.0 63 8915.0 64 5929.0 65 4238.5 66 3050.0 67 2221.0 68 1563.0 69 1057.0 70 756.0 71 590.0 72 433.0 73 325.5 74 238.5 75 175.0 76 130.0 77 63.0 78 30.5 79 20.5 80 16.0 81 9.5 82 7.0 83 6.0 84 7.0 85 6.5 86 4.5 87 3.0 88 5.0 89 4.5 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03906948507921338 2 0.03914461870436571 3 0.021863884919329026 4 0.04245049821106839 5 0.06288684425250309 6 0.06348791325372174 7 0.06303711150280775 8 0.06716946088618608 9 0.06453978400585442 10-11 0.07107640939410742 12-13 0.07077587489349808 14-15 0.06608002332147725 16-17 0.05447187823544173 18-19 0.04662041440702289 20-21 0.05443431142286557 22-23 0.029752915560324035 24-25 0.015815628094566186 26-27 0.013110817589082183 28-29 0.015702927656837687 30-31 0.011645711898611681 32-33 0.006611759013405342 34-35 0.010330873458445845 36-37 0.00593555638703434 38-39 0.0074006620775048425 40-41 0.007513362515233342 42-43 0.0048836856349016725 44-45 0.0010143039395565012 46-47 0.0029302113809410034 48-49 7.88903064099501E-4 50-51 0.005860422761882007 52-53 0.004808552009749339 54-55 0.0069498603265908425 56-57 0.02208928579478603 58-59 0.009279002706313178 60-61 0.019910410665368358 62-63 0.013411352089691516 64-65 0.009880071707531845 66-67 0.015702927656837687 68-69 0.024493561799660696 70-71 0.01874583947550719 72-73 0.01795693641140769 74-75 0.01758126828564602 76-77 0.026108934740435862 78-79 0.03707844401267655 80-81 0.031293154875946874 82-83 0.030353984561542705 84-85 0.020023111103096857 86-87 0.02389249279844203 88-89 0.018896106725811854 90-91 0.016980199284427355 92-93 0.030804786312456704 94-95 0.021563350418719693 96-97 0.03696574357494804 98-99 0.05368297517134224 100-101 0.06363818050402642 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1330962.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.10947772018643 #Duplication Level Percentage of deduplicated Percentage of total 1 71.6982091578714 34.49363396057881 2 14.427121273833585 13.88162538979849 3 5.7431917472268585 8.289058662179075 4 2.768013040925861 5.326706468864329 5 1.5131381292447736 3.6398142558233 6 0.9328634169027281 2.692774306287527 7 0.5800736352398334 1.9534927741446821 8 0.4176723857728286 1.6075200270140009 9 0.3036329045845207 1.3146858412402043 >10 1.4466206346834856 12.441512959355784 >50 0.10776851902953423 3.5674804215528355 >100 0.05466661103001086 4.7153269615910025 >500 0.004060936333826776 1.3312930359525799 >1k 0.002342847884900063 2.124192743316785 >5k 4.685695769800126E-4 1.603082103573536 >10k+ 1.5618985899333752E-4 1.017800088727081 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13545 1.0176849526883562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9389 0.7054296065552584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6392 0.4802541319737152 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5553 0.41721702047090753 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4296 0.3227740536544244 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3558 0.26732543829200234 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2104 0.1580811473205095 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2030 0.15252125905923686 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 0.14485762929369886 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1891 0.14207768516306252 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1710 0.12847849901049013 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1548 0.11630685173581214 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1547 0.1162317181106598 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1502 0.1128507049788048 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1431 0.10751621759298913 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1356 0.10188119570656412 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.5026725030466685E-4 0.0 0.0 0.0 0.0 7 1.5026725030466685E-4 0.0 0.0 0.0 0.0 8 1.5026725030466685E-4 0.0 0.0 0.0 0.0 9 1.5026725030466685E-4 0.0 0.0 0.0 0.0 10-11 1.5026725030466685E-4 0.0 0.0 0.0 0.0 12-13 1.5026725030466685E-4 0.0 0.0 3.756681257616671E-5 0.0 14-15 3.005345006093337E-4 0.0 0.0 7.513362515233342E-5 0.0 16-17 3.005345006093337E-4 0.0 0.0 1.1270043772850014E-4 0.0 18-19 5.25935376066334E-4 0.0 0.0 1.5026725030466685E-4 0.0 20-21 7.513362515233343E-4 0.0 0.0 2.62967688033167E-4 0.0 22-23 7.513362515233343E-4 0.0 0.0 7.137694389471675E-4 0.0 24-25 8.264698766756676E-4 0.0 0.0 0.0013524052527420015 0.0 26-27 8.264698766756676E-4 0.0 0.0 0.0024042760048746696 0.0 28-29 9.016035018280011E-4 0.0 0.0 0.0048836856349016725 0.0 30-31 9.767371269803345E-4 0.0 0.0 0.008715500517670678 0.0 32-33 9.767371269803345E-4 0.0 0.0 0.013674319777724684 0.0 34-35 0.001051870752132668 0.0 0.0 0.018933673538388023 0.0 36-37 0.0011270043772850014 0.0 0.0 0.0252824648637602 0.0 38-39 0.001427538877894335 0.0 0.0 0.03546307107190137 0.0 40-41 0.001427538877894335 0.0 0.0 0.0550729472366604 0.0 42-43 0.001427538877894335 0.0 0.0 0.07254151508457793 0.0 44-45 0.0017656401910798355 0.0 0.0 0.09192599037387994 0.0 46-47 0.0021037415042653358 0.0 0.0 0.13178437851719282 0.0 48-49 0.002254008754570003 0.0 0.0 0.15950868619840386 0.0 50-51 0.002254008754570003 0.0 0.0 0.19185371182648342 0.0 52-53 0.002254008754570003 0.0 0.0 0.22893215583915993 0.0 54-55 0.0023291423797223362 0.0 0.0 0.2770928095618057 0.0 56-57 0.0025545432551793363 0.0 0.0 0.39475206655035977 0.0 58-59 0.0025545432551793363 0.0 0.0 0.5160928711713784 0.0 60-61 0.0025921100677555034 3.756681257616671E-5 0.0 0.617635965564757 0.0 62-63 0.00262967688033167 7.513362515233342E-5 0.0 0.732815812923284 0.0 64-65 0.0027799441306363367 7.513362515233342E-5 0.0 0.8415341685187105 0.0 66-67 0.0029302113809410034 7.513362515233342E-5 0.0 1.0041233333483601 0.0 68-69 0.0029302113809410034 7.513362515233342E-5 0.0 1.2082238260746738 0.0 70-71 0.00296777819351717 7.513362515233342E-5 0.0 1.3219385677427304 0.0 72-73 0.003005345006093337 7.513362515233342E-5 0.0 1.4450450125548286 0.0 74-75 0.003042911818669504 7.513362515233342E-5 0.0 1.6559075315448526 0.0 76-77 0.0030804786312456705 7.513362515233342E-5 0.0 1.8760114864286133 0.0 78-79 0.003118045443821837 7.513362515233342E-5 0.0 2.0992710535687724 0.0 80-81 0.003268312694126504 7.513362515233342E-5 0.0 2.3005916021644497 0.0 82-83 0.0033810131318550043 7.513362515233342E-5 0.0 2.582229996047971 0.0 84-85 0.0034937135695835043 7.513362515233342E-5 0.0 2.9602272641893608 0.0 86-87 0.0036064140073120044 7.513362515233342E-5 0.0 3.3907429363122312 0.0 88-89 0.0036064140073120044 7.513362515233342E-5 0.0 3.8854227243151946 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19345 0.0 25.385302 1 GTACATG 11130 0.0 25.114983 1 GAGTACT 9165 0.0 24.845844 12-13 TACATGG 11335 0.0 24.534943 2 ACATGGG 11395 0.0 23.3193 3 AGTACTT 9350 0.0 23.083143 12-13 GTACTTT 10225 0.0 22.315792 14-15 CATGGGA 8065 0.0 21.866919 4 TATCAAC 22420 0.0 21.712763 2 ATCAACG 22520 0.0 21.635828 3 GCGCTAT 55 0.0028894413 21.576603 40-41 GGAGTCG 155 3.880063E-5 21.470877 8 GGTATCA 14255 0.0 21.343393 1 TCAACGC 22785 0.0 21.321606 4 CAACGCA 22880 0.0 21.19551 5 AACGCAG 23130 0.0 21.027296 6 ACCGCCG 70 4.8529822E-4 20.357805 86-87 ACTTTTT 11595 0.0 20.024553 16-17 ATGGGAT 3100 0.0 19.937992 5 ACGCAGA 24630 0.0 19.727402 7 >>END_MODULE