FastQCFastQC Report
Thu 26 May 2016
SRR937110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330962
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144741.087484090454874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110350.8290995535559993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77930.5855163408121344No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA61830.4645512043168776No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44370.3333678948009034No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30290.22757975058641794No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22460.16875012209214085No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21590.16221349670388785No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20140.1513191210567995No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT19970.15004184942920984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19810.14883971142677252No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19500.14651056904705018No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG18330.13771993490422715No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA17780.13358758552084882No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17070.12825309813503316No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC15380.1155555154842888No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG13980.10503680796296214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA753.0800083E-431.6643892
TCGACCG404.4941404E-429.6887142-43
ATCGGCG350.00839954727.13886350-51
GAGTACT108200.023.90013312-13
GTACATG111400.023.4995371
AGTACTT111050.023.15845712-13
TACATGG112800.022.779832
GTATCAA217950.022.6273351
CATATAG3150.022.616573
GTACTTT117300.022.0480614-15
ACATGGG113250.021.7658273
GGTATCA167350.021.2358071
GCTTATA8750.021.176271
CATGGGA79500.020.0120284
TACTTTT128150.019.92188314-15
ACTTTTT133200.019.4878816-17
ATCAACG254700.019.2440643
TATCAAC256000.019.1841662
TCAACGC256600.019.0816294
CAACGCA258200.018.9633835
Produced by FastQC (version 0.11.5)