##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937110_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1330962 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08465831481289 31.0 31.0 34.0 30.0 34.0 2 32.13880486445143 33.0 31.0 34.0 30.0 34.0 3 32.251577430460074 33.0 31.0 34.0 30.0 34.0 4 35.97454021978088 37.0 35.0 37.0 35.0 37.0 5 35.88637316467337 37.0 35.0 37.0 35.0 37.0 6 35.938113935634526 37.0 35.0 37.0 35.0 37.0 7 35.88548433388782 37.0 35.0 37.0 35.0 37.0 8 35.88257515992192 37.0 35.0 37.0 35.0 37.0 9 37.524492059127155 39.0 37.0 39.0 35.0 39.0 10-11 37.42973766343442 39.0 37.0 39.0 34.0 39.0 12-13 37.34798589291054 39.0 37.0 39.0 34.0 39.0 14-15 38.44967624920922 40.0 38.0 41.0 34.0 41.0 16-17 38.40339431178351 40.0 38.0 41.0 34.0 41.0 18-19 38.52919617539794 40.0 38.0 41.0 34.0 41.0 20-21 38.642113749303135 40.0 38.0 41.0 34.0 41.0 22-23 38.57425268339742 40.0 38.0 41.0 34.0 41.0 24-25 38.47579570265718 40.0 38.0 41.0 34.0 41.0 26-27 38.25249894437256 40.0 38.0 41.0 34.0 41.0 28-29 38.07743459242262 40.0 37.0 41.0 33.0 41.0 30-31 37.91746233175703 40.0 37.0 41.0 33.0 41.0 32-33 37.731681295183485 40.0 36.5 41.0 33.0 41.0 34-35 37.564166745556975 40.0 36.0 41.0 33.0 41.0 36-37 37.32461520313878 40.0 36.0 41.0 33.0 41.0 38-39 37.12474172816354 40.0 35.5 41.0 32.5 41.0 40-41 36.83180774507461 39.0 35.0 41.0 31.0 41.0 42-43 36.514696137079795 39.0 35.0 41.0 31.0 41.0 44-45 36.10346876920603 39.0 35.0 41.0 30.0 41.0 46-47 35.51177419039762 39.0 35.0 41.0 27.5 41.0 48-49 34.958499566478984 39.0 35.0 40.5 24.0 41.0 50-51 34.64955348086572 39.0 35.0 40.0 23.0 41.0 52-53 34.38246321082045 38.0 34.0 40.0 22.5 41.0 54-55 34.10840166736541 38.0 34.0 40.0 21.0 41.0 56-57 33.876507744022746 38.0 34.0 40.0 19.5 41.0 58-59 33.54931808721811 38.0 33.5 40.0 18.0 41.0 60-61 33.21300194896624 37.0 33.0 40.0 15.0 41.0 62-63 32.8703095956158 37.0 33.0 40.0 12.5 41.0 64-65 32.43809627923262 36.0 32.0 39.0 8.0 40.5 66-67 32.02075679095271 36.0 31.5 39.0 7.5 40.0 68-69 31.59554592843372 35.0 31.0 38.5 4.0 40.0 70-71 31.208477777727687 35.0 31.0 38.0 2.0 40.0 72-73 30.835653084009913 35.0 30.5 37.0 2.0 39.5 74-75 30.365910146195006 35.0 30.0 37.0 2.0 39.0 76-77 28.89542676650423 33.0 28.5 35.0 2.0 37.5 78-79 29.567830636787527 34.0 29.0 36.0 2.0 38.0 80-81 29.28479475747617 34.0 29.0 35.5 2.0 37.0 82-83 29.10516378378947 34.0 29.0 35.0 2.0 37.0 84-85 28.79250008640367 34.0 29.0 35.0 2.0 36.5 86-87 28.414525358349827 34.0 29.0 35.0 2.0 36.0 88-89 28.026269345030137 34.0 28.5 35.0 2.0 36.0 90-91 27.575189224034947 33.5 27.0 35.0 2.0 35.0 92-93 27.205618567622516 33.0 26.0 35.0 2.0 35.0 94-95 26.752972661879152 33.0 25.5 35.0 2.0 35.0 96-97 26.14752111630535 33.0 25.0 35.0 2.0 35.0 98-99 25.42041057520801 32.5 22.0 34.0 2.0 35.0 100-101 22.504261954886765 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25917969275849373 1101 2 0.2099911654140243 1101 3 0.20842078095208194 1101 4 0.20662078407856654 1101 5 0.21454449433145584 1101 6 0.24636221971240957 1101 7 0.166915774277534 1101 8 0.18652866079190744 1101 9 0.23769278320220621 1101 10-11 0.2562731030902441 1101 12-13 0.266113496178896 1101 14-15 0.35346268143822357 1101 16-17 0.34968416117141743 1101 18-19 0.2715140913030396 1101 20-21 0.24830355136806048 1101 22-23 0.22906314878150624 1101 24-25 0.15099016634899698 1101 26-27 0.1866712112292177 1101 28-29 0.08455472958794985 1101 30-31 0.18573983941018213 1101 32-33 0.4417543428528674 1101 34-35 0.3416526865274676 1101 36-37 0.4448630467645458 1101 38-39 0.2953104344901405 1101 40-41 0.5118272545227001 1101 42-43 0.4256425193058533 1101 44-45 0.3985031829657757 1101 46-47 0.6198973269132253 1101 48-49 0.8379239981294546 1101 50-51 0.5402315907657425 1101 52-53 0.7063233206770789 1101 54-55 0.5968491819938748 1101 56-57 0.60897655513822 1101 58-59 0.6145660809921765 1101 60-61 0.4971623632670088 1101 62-63 0.579403402159933 1101 64-65 0.4747284016464519 1101 66-67 0.49361095594192506 1101 68-69 0.5276886129202474 1101 70-71 0.4616116581504741 1101 72-73 0.5364717717002598 1101 74-75 0.5106457492310135 1101 76-77 0.5954150782721328 1101 78-79 0.7721326097079455 1101 80-81 1.039236563898001 1101 82-83 0.8388986877723816 1101 84-85 0.7265851961399648 1101 86-87 0.5877287308198511 1101 88-89 0.5178690250938089 1101 90-91 0.43270304425096384 1101 92-93 0.5335736304164627 1101 94-95 0.6827177548496799 1101 96-97 0.6682588733116894 1101 98-99 0.781222949513225 1101 100-101 1.001600106323881 1102 1 0.20790811858476843 1102 2 0.23838900785278128 1102 3 0.10757105057530225 1102 4 0.12587157971164942 1102 5 0.12432826341657943 1102 6 0.1476055222946968 1102 7 0.15090419803200206 1102 8 0.15551551630033345 1102 9 0.1271915055852446 1102 10-11 0.1850520724792304 1102 12-13 0.1959690161172034 1102 14-15 0.21544156335073694 1102 16-17 0.23741321141859828 1102 18-19 0.21744161531589867 1102 20-21 0.19560814647282854 1102 22-23 0.3261132995633318 1102 24-25 0.2904489632021452 1102 26-27 0.30294118838174455 1102 28-29 0.35650082220942636 1102 30-31 0.5334512792614632 1102 32-33 0.4329527418031418 1102 34-35 0.508456874463775 1102 36-37 0.581558349507965 1102 38-39 0.5707683551397622 1102 40-41 0.6126111617285801 1102 42-43 0.4727794631540121 1102 44-45 0.5891954269100452 1102 46-47 0.7343841435751912 1102 48-49 0.9422781829004947 1102 50-51 0.8781501254196655 1102 52-53 0.6789822458674237 1102 54-55 0.7964072315562092 1102 56-57 0.6937174132709387 1102 58-59 0.8225068732297842 1102 60-61 0.680250951903119 1102 62-63 0.6168033725215238 1102 64-65 0.649115562747042 1102 66-67 0.513717416827852 1102 68-69 0.6719855926798068 1102 70-71 0.5327089555877613 1102 72-73 0.47541754347873777 1102 74-75 0.5624613537107344 1102 76-77 0.6075979400765661 1102 78-79 0.5232199509410158 1102 80-81 0.4549118664687768 1102 82-83 0.47243933411995087 1102 84-85 0.2972943886515189 1102 86-87 0.3017615834162335 1102 88-89 0.376300649566506 1102 90-91 0.5098397075413459 1102 92-93 0.686897556671493 1102 94-95 0.8179559555966804 1102 96-97 0.6705026161538719 1102 98-99 0.7860652688484038 1102 100-101 0.790486313528028 1103 1 0.1653721394968528 1103 2 0.13235394706973835 1103 3 0.09872568534261461 1103 4 0.1330989792082775 1103 5 0.21283779143702475 1103 6 0.2509536910295722 1103 7 0.18091187486642468 1103 8 0.1551656858709194 1103 9 0.19655996448503288 1103 10-11 0.13377276203387822 1103 12-13 0.15288278291635038 1103 14-15 0.2238314202117877 1103 16-17 0.2542949590159438 1103 18-19 0.40442233577223163 1103 20-21 0.30250806581747725 1103 22-23 0.16357819505149251 1103 24-25 0.09901033414872984 1103 26-27 0.15818305725363757 1103 28-29 0.18653910920642147 1103 30-31 0.23668939717375537 1103 32-33 0.2574872729817841 1103 34-35 0.37533910793671765 1103 36-37 0.3330633491678512 1103 38-39 0.3703847563564011 1103 40-41 0.41483792958693755 1103 42-43 0.36414010917420114 1103 44-45 0.44923531729651245 1103 46-47 0.44741872820014095 1103 48-49 0.43132654695735795 1103 50-51 0.48034919836902645 1103 52-53 0.4506717324912657 1103 54-55 0.32816284584872335 1103 56-57 0.3889106459956295 1103 58-59 0.534869734842701 1103 60-61 0.23495325592519123 1103 62-63 0.43149649349482644 1103 64-65 0.39119943459249384 1103 66-67 0.28562554747008306 1103 68-69 0.34114925501569715 1103 70-71 0.29816465830963423 1103 72-73 0.5467865951467452 1103 74-75 0.5990198482737839 1103 76-77 0.5803524660077457 1103 78-79 0.564430704339717 1103 80-81 0.8173118975648137 1103 82-83 0.7315604337536001 1103 84-85 0.5528880314552147 1103 86-87 0.48263363293667183 1103 88-89 0.376542372675722 1103 90-91 0.4168091070962845 1103 92-93 0.31098532523922984 1103 94-95 0.31298423355639216 1103 96-97 0.49649720349972526 1103 98-99 0.5671795273536553 1103 100-101 0.6667289403658536 1104 1 0.0982895511292341 1104 2 0.05344284211421524 1104 3 0.05046239977102829 1104 4 0.06555192395630627 1104 5 -0.03822124901915913 1104 6 0.07527398851221534 1104 7 -0.036394273164205515 1104 8 0.012964672437753677 1104 9 0.06707722950255857 1104 10-11 0.05559613031414301 1104 12-13 0.0940371370135864 1104 14-15 -0.01624724536925015 1104 16-17 0.12501228055293723 1104 18-19 0.01545265482740632 1104 20-21 0.08675550355513195 1104 22-23 0.13944690201485344 1104 24-25 0.05024026204443999 1104 26-27 0.2121182249513609 1104 28-29 0.015368372858532098 1104 30-31 0.22221502819119365 1104 32-33 0.2768406427449932 1104 34-35 0.29252829984827855 1104 36-37 0.3141800422755594 1104 38-39 0.40560414700942715 1104 40-41 0.5657987604790193 1104 42-43 0.548111664774531 1104 44-45 0.7714413811075502 1104 46-47 0.8896660484220789 1104 48-49 0.9911679287183617 1104 50-51 0.9707646723910557 1104 52-53 1.084509544012846 1104 54-55 1.0024264140176342 1104 56-57 0.950855419204288 1104 58-59 0.9947118860518529 1104 60-61 1.0788511123825444 1104 62-63 0.8425829123152511 1104 64-65 0.8221760881365654 1104 66-67 0.9283658741078114 1104 68-69 0.9840522150127846 1104 70-71 1.0369565967313967 1104 72-73 1.0581768760372903 1104 74-75 1.0558121868085841 1104 76-77 1.084583731344246 1104 78-79 1.2768456103691115 1104 80-81 1.3599232367123761 1104 82-83 1.2864427221763428 1104 84-85 1.2015100897150326 1104 86-87 1.0691398906486427 1104 88-89 1.0072852538813208 1104 90-91 1.1562711649084179 1104 92-93 1.2483914175897937 1104 94-95 1.3160896563471631 1104 96-97 1.441172132640549 1104 98-99 1.6148227076647395 1104 100-101 1.5019390276912254 1105 1 0.07000764042786756 1105 2 0.06648515635098562 1105 3 0.05549943394468926 1105 4 0.029657731381469432 1105 5 0.12843289038281114 1105 6 0.09204513256645441 1105 7 0.0206706388263882 1105 8 0.047973366359450154 1105 9 0.08560219061435248 1105 10-11 0.09115584492158035 1105 12-13 0.032701680487853935 1105 14-15 0.1856564693584417 1105 16-17 0.09143151584574838 1105 18-19 0.11526936304880309 1105 20-21 0.13954289153453914 1105 22-23 0.015119526648618375 1105 24-25 0.12729421446803713 1105 26-27 0.15383405218391744 1105 28-29 0.09528945824937551 1105 30-31 -0.009627059552634876 1105 32-33 -0.05068415587028596 1105 34-35 -0.08187173365320888 1105 36-37 -0.04678396295582132 1105 38-39 0.014021896210721252 1105 40-41 0.06983790853911387 1105 42-43 0.02566280643785035 1105 44-45 0.04437057056488669 1105 46-47 5.24824630012688E-4 1105 48-49 -0.04464877377628795 1105 50-51 -0.17447643450373818 1105 52-53 -0.13042234703861055 1105 54-55 -0.15963415638093892 1105 56-57 -0.14975945473921826 1105 58-59 -0.03068105844950253 1105 60-61 -0.02514057524044233 1105 62-63 -0.03936157686943176 1105 64-65 -0.033275562706371886 1105 66-67 -0.21619052829083074 1105 68-69 -0.1547788694975729 1105 70-71 -0.22479254586681563 1105 72-73 -0.19293733467702623 1105 74-75 -0.15800012470081626 1105 76-77 -0.1608937471392835 1105 78-79 -0.08217487922502897 1105 80-81 0.04175809443486145 1105 82-83 0.01618040841202628 1105 84-85 -0.23654312324202564 1105 86-87 -0.2625264216753962 1105 88-89 -0.16371280403473065 1105 90-91 -0.22384362778682743 1105 92-93 -0.085262971641626 1105 94-95 -0.11305917686339839 1105 96-97 0.2149010984683173 1105 98-99 0.41887713898776724 1105 100-101 0.6362250416086574 1106 1 -0.02113351586690726 1106 2 -0.017426672036044977 1106 3 0.022700893541831135 1106 4 -0.04939740232676826 1106 5 0.009270725553832904 1106 6 0.01319634741544462 1106 7 -0.025551062813853775 1106 8 -0.025097659189128763 1106 9 -0.0782329361380647 1106 10-11 -0.025695591245614935 1106 12-13 0.004694178404676563 1106 14-15 0.08547499518183344 1106 16-17 -0.05264142199118993 1106 18-19 0.100580347207611 1106 20-21 -0.044444798085713444 1106 22-23 0.06954283308034803 1106 24-25 0.002705457763120478 1106 26-27 0.08359300330295127 1106 28-29 0.16031495355156977 1106 30-31 0.04441637794419506 1106 32-33 0.04788704920153464 1106 34-35 0.0326946736270699 1106 36-37 0.05274394906314939 1106 38-39 0.08513955128072581 1106 40-41 0.2701297604348838 1106 42-43 -0.02452078750120279 1106 44-45 0.08477249253287056 1106 46-47 0.32514430851100684 1106 48-49 0.29643882568841207 1106 50-51 0.09532693032839745 1106 52-53 0.22313921030021788 1106 54-55 0.20676078050078672 1106 56-57 0.2821241361608884 1106 58-59 0.42654363599518064 1106 60-61 0.3646640426901939 1106 62-63 0.49488946978673454 1106 64-65 0.46342117207157685 1106 66-67 0.2738415232677909 1106 68-69 0.48986321517812215 1106 70-71 0.545326344668279 1106 72-73 0.651421639778146 1106 74-75 0.6026923885608291 1106 76-77 0.35583643111210606 1106 78-79 0.4818800274997983 1106 80-81 0.8955704590655174 1106 82-83 0.6909861500043561 1106 84-85 0.4216688177362826 1106 86-87 0.4442265787264219 1106 88-89 0.4950155711054478 1106 90-91 0.3904987975140557 1106 92-93 0.3965534059056992 1106 94-95 0.5149996122157354 1106 96-97 0.5374439655051866 1106 98-99 0.6353545965334284 1106 100-101 0.43180734410588784 1107 1 0.09829290087632359 1107 2 0.02452894178036047 1107 3 0.026421264876709927 1107 4 0.06697110014183494 1107 5 0.03169243916404696 1107 6 -0.030722058786210482 1107 7 0.04368701403194564 1107 8 0.07098787501929138 1107 9 0.009301908205983977 1107 10-11 -0.005825390694347732 1107 12-13 0.043201933404795057 1107 14-15 0.11601863522342626 1107 16-17 0.1479787049212291 1107 18-19 0.0679750143892619 1107 20-21 0.13268053621516884 1107 22-23 0.03930174630744432 1107 24-25 0.10003592747170131 1107 26-27 0.05989845921035197 1107 28-29 -0.004657531852330976 1107 30-31 0.008300178762318922 1107 32-33 0.18094240806170347 1107 34-35 0.1283186726751282 1107 36-37 0.04072511312271132 1107 38-39 0.20049466610856825 1107 40-41 0.30164328755056147 1107 42-43 0.26334743114083636 1107 44-45 0.4343446069140029 1107 46-47 0.6734800704634196 1107 48-49 0.6735744986889927 1107 50-51 0.5883832255236285 1107 52-53 0.439366677299283 1107 54-55 0.6300205139964206 1107 56-57 0.6367233945054878 1107 58-59 0.6721228665228267 1107 60-61 0.5077873443636989 1107 62-63 0.7344072962409314 1107 64-65 0.5012206286070935 1107 66-67 0.6460905025650305 1107 68-69 0.764809034539585 1107 70-71 0.4723717978837101 1107 72-73 0.5794773595174583 1107 74-75 0.5176390272154698 1107 76-77 0.4991093513266023 1107 78-79 0.6300345696026888 1107 80-81 0.792108165083615 1107 82-83 0.7613737732153218 1107 84-85 0.7080962507932007 1107 86-87 0.6117536983061669 1107 88-89 0.5587111295610399 1107 90-91 0.5871870974221807 1107 92-93 0.5829742065892276 1107 94-95 0.7176835776727728 1107 96-97 0.984462030919893 1107 98-99 1.1018273638131966 1107 100-101 1.4062395446688605 1108 1 -0.059276911746415806 1108 2 -0.047924534859170365 1108 3 -0.12336580185976231 1108 4 -0.05341733506446644 1108 5 -0.014185602130758923 1108 6 -0.026604212310793685 1108 7 -0.014088811272515045 1108 8 -0.006871679295244348 1108 9 -0.05293598729523552 1108 10-11 -0.08616191150725427 1108 12-13 -0.13643731371614365 1108 14-15 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-1.1208153851250628 2316 82-83 -1.124892863362195 2316 84-85 -1.3907320880002736 2316 86-87 -1.3968315668285527 2316 88-89 -1.2807570187369777 2316 90-91 -1.368155413102535 2316 92-93 -1.3263163378947311 2316 94-95 -1.1948124990649411 2316 96-97 -1.3014134466805487 2316 98-99 -1.5641418051814888 2316 100-101 -2.1374701365158266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 13.0 5 40.0 6 90.0 7 280.0 8 534.0 9 1005.0 10 1849.0 11 3282.0 12 5069.0 13 7068.0 14 8946.0 15 12815.0 16 17676.0 17 24580.0 18 21227.0 19 10819.0 20 8214.0 21 8253.0 22 8179.0 23 8844.0 24 10168.0 25 11658.0 26 14257.0 27 17084.0 28 21111.0 29 25828.0 30 31542.0 31 40169.0 32 50414.0 33 64924.0 34 88598.0 35 123874.0 36 183565.0 37 273806.0 38 208733.0 39 16443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88374609768965 19.880401797054308 15.438343872264248 19.797508232991788 2 15.18473546746654 22.28539698664639 40.332532858057405 22.19733468782967 3 19.034193801443614 28.2340441199676 31.51591567943518 21.21584639915361 4 12.097198941216158 18.003683053072177 38.56273775114372 31.336380254567942 5 12.174739554961135 39.776391075178736 33.231321779704636 14.817547590155497 6 28.21613239145821 38.261873742450945 18.118774239985814 15.403219626105027 7 25.75069761570954 36.55183243398384 20.780307777382074 16.917162172924545 8 26.45496483741059 33.3870289114624 20.151770150868543 20.00623610025846 9 27.887047113290986 16.429169277560142 20.98166589279033 34.70211771635854 10-11 24.125707570914873 27.851433774968783 27.001108972307247 21.021749681809098 12-13 26.722656307230757 26.043034334978387 27.84090887746105 19.393400480329806 14-15 23.514194995800032 25.943490497850423 28.701232642254247 21.841081864095294 16-17 19.99854992048981 29.53455077400811 28.94328648875268 21.523612816749395 18-19 19.842467562376 27.305948115580392 33.49074555160209 19.360838770441514 20-21 21.84725289710982 25.749349049561644 33.115279180818646 19.288118872509887 22-23 21.011606642413533 26.02057759725672 32.90841511628431 20.059400644045436 24-25 20.720330816593577 26.16759405464845 33.560399687877684 19.551675440880285 26-27 20.851557340157832 25.998222243594704 33.59786789451578 19.55235252173168 28-29 20.08185665992668 26.278024109168264 33.82354156663759 19.81657766426747 30-31 20.215685080842448 26.566061729313645 33.581989717042035 19.63626347280187 32-33 20.106584961046824 26.607471165484686 33.12962026571858 20.15632360774991 34-35 20.672661323194887 26.27267484780122 33.00924369274348 20.045420136260407 36-37 21.034897225287537 26.02422467622593 33.106807023555504 19.834071074931025 38-39 21.1073428752729 26.14193888695405 33.120426129991735 19.630292107781315 40-41 20.539794009953106 26.386811046548765 33.22819931156314 19.845195631934995 42-43 20.360966208258116 26.908475455011654 32.43496277817747 20.295595558552755 44-45 20.68073084646176 27.16784786492076 31.75481667790574 20.396604610711744 46-47 20.909130184061294 27.185246545211385 30.70226549982437 21.20335777090295 48-49 21.39319107596147 27.402006620110757 30.168951447637372 21.0358508562904 50-51 21.61992091202872 27.844097667558533 29.89872441906857 20.63725700134418 52-53 21.316342611985295 28.227864507512646 29.695868842851482 20.759924037650578 54-55 20.748036347297923 27.975232505195585 29.91146093957795 21.365270207928543 56-57 21.335922470153736 28.616611273714813 28.95682054599531 21.09064571013614 58-59 21.345614827955014 27.91229994015533 29.31780452317544 21.424280708714218 60-61 21.397417807570765 27.669760669350442 29.810918718941636 21.121902804137157 62-63 21.959968008066355 27.528390211430015 29.617821114901606 20.89382066560202 64-65 21.551416131580933 27.674818153504326 29.89206615414151 20.881699560773228 66-67 21.06884676604692 28.031400163116754 29.647465698334685 21.25228737250164 68-69 21.284868538944682 28.277716856174802 29.389295053779435 21.048119551101077 70-71 21.29716558488763 28.245308940991105 29.05933874542183 21.398186728699432 72-73 21.30499095092157 28.12691027229141 28.84970940379028 21.71838937299674 74-75 21.21202330484596 28.38936885463925 28.845194389885805 21.55341345062898 76-77 21.488715448987065 28.144412198397717 29.043381890244476 21.323490462370742 78-79 21.073422108273967 28.22424695779327 29.080148795647194 21.622182138285574 80-81 21.039550399711487 28.672724950411734 28.649809160305345 21.637915489571437 82-83 20.948027972280183 28.50346647992432 28.547271786001687 22.00123376179381 84-85 20.99796762475065 28.073638853304583 28.514262315405105 22.414131206539665 86-87 21.14433019124513 28.016314515365575 28.619637525338813 22.21971776805048 88-89 21.51755217717323 27.640302461557418 28.57858245188009 22.26356290938926 90-91 20.7460034990163 27.932097895819176 29.195673301423685 22.12622530374084 92-93 20.566696219270302 28.245965318266514 28.831858207609546 22.355480254853642 94-95 20.438835602132006 28.29635734969052 28.54816920795159 22.716637840225886 96-97 20.652004045206535 28.04930734102804 28.202655530828167 23.096033082937254 98-99 20.66412263638474 27.900821888578086 28.20083113003481 23.234224345002364 100-101 20.66776262404655 28.100637739001854 28.289863195667465 22.94173644128413 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1780.0 1 1260.0 2 788.0 3 849.0 4 748.0 5 751.0 6 1294.0 7 2681.5 8 4215.5 9 4735.0 10 4096.0 11 3443.0 12 3499.0 13 3655.5 14 3794.0 15 3964.5 16 4101.5 17 4270.0 18 4455.0 19 4635.0 20 4926.0 21 5357.5 22 5925.5 23 6831.0 24 7760.5 25 8975.0 26 11016.5 27 13250.5 28 14805.5 29 16413.0 30 18552.0 31 20910.5 32 23790.5 33 27059.0 34 31114.0 35 33793.0 36 35486.5 37 38319.5 38 39142.5 39 38448.0 40 38640.0 41 39657.5 42 41186.0 43 42783.5 44 44697.5 45 45574.5 46 45566.0 47 48446.0 48 65410.0 49 64802.0 50 46794.5 51 41327.0 52 36947.0 53 34698.5 54 33021.0 55 30171.5 56 27543.5 57 24691.0 58 22558.0 59 20905.0 60 18336.0 61 15248.0 62 12209.0 63 8841.5 64 5889.0 65 4227.0 66 3038.5 67 2245.0 68 1607.5 69 1036.5 70 736.0 71 585.0 72 445.5 73 356.0 74 272.5 75 205.5 76 138.0 77 48.0 78 29.5 79 20.5 80 8.5 81 5.5 82 5.5 83 3.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025545432551793364 2 0.006461491763100674 3 0.010218173020717345 4 7.513362515233342E-5 5 0.005033952885206339 6 0.0 7 0.0 8 1.5026725030466685E-4 9 0.0 10-11 0.0 12-13 1.1270043772850015E-4 14-15 0.0 16-17 3.756681257616671E-5 18-19 0.004620717946868505 20-21 0.0017656401910798355 22-23 0.0 24-25 0.005522321448696507 26-27 0.005334487385815673 28-29 0.007438228890081009 30-31 0.030879919937609034 32-33 3.7566812576166715E-4 34-35 0.004094782570802172 36-37 0.01589076171971852 38-39 0.007550929327809508 40-41 0.009091168643432344 42-43 0.0067244594511338416 44-45 0.00296777819351717 46-47 0.0014651056904705017 48-49 0.0013148384401658348 50-51 0.0029302113809410034 52-53 0.00296777819351717 54-55 0.0018032070036560024 56-57 0.0012397048150135017 58-59 0.0012397048150135017 60-61 0.0 62-63 1.1270043772850015E-4 64-65 0.007550929327809508 66-67 0.0010143039395565012 68-69 0.0012397048150135017 70-71 0.003944515320497504 72-73 0.012284347712406513 74-75 0.006010690012186674 76-77 0.0037191144450405044 78-79 0.010781675209359846 80-81 1.5026725030466685E-4 82-83 0.00548475463612034 84-85 5.259353760663339E-4 86-87 0.0 88-89 0.0012021380024373348 90-91 0.0012397048150135017 92-93 1.5026725030466685E-4 94-95 6.010690012186674E-4 96-97 3.005345006093337E-4 98-99 2.254008754570003E-4 100-101 7.513362515233343E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1330962.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.18957778390765 #Duplication Level Percentage of deduplicated Percentage of total 1 70.15043434561387 32.40218943781639 2 15.198165435874959 14.039936891460863 3 5.984999203268547 8.293337587079936 4 2.9197043465907186 5.39439644091461 5 1.6221455973106185 3.7463110121901075 6 0.9704560034514085 2.689497183436739 7 0.6365446580567048 2.058121029736874 8 0.44566426826358674 1.646803550757538 9 0.32720271751364666 1.3602019834532293 >10 1.5661105400089506 13.006923673879292 >50 0.11161554520841918 3.57320884809778 >100 0.05996908426723483 5.205093715821156 >500 0.0034128685116086126 1.1029472258970887 >1k 0.0029253158670930965 2.517043723647674 >5k 3.250350963436774E-4 1.0491247824908263 >10k+ 3.250350963436774E-4 1.914862913319869 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14474 1.087484090454874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11035 0.8290995535559993 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7793 0.5855163408121344 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6183 0.4645512043168776 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4437 0.3333678948009034 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3029 0.22757975058641794 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2246 0.16875012209214085 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2159 0.16221349670388785 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2014 0.1513191210567995 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1997 0.15004184942920984 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1981 0.14883971142677252 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1950 0.14651056904705018 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1833 0.13771993490422715 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1778 0.13358758552084882 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1707 0.12825309813503316 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1538 0.1155555154842888 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1398 0.10503680796296214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.513362515233342E-5 0.0 0.0 0.0 0.0 6 7.513362515233342E-5 0.0 0.0 0.0 0.0 7 1.5026725030466685E-4 0.0 0.0 0.0 0.0 8 1.5026725030466685E-4 0.0 0.0 0.0 0.0 9 1.5026725030466685E-4 0.0 0.0 0.0 0.0 10-11 3.7566812576166715E-4 0.0 0.0 0.0 0.0 12-13 3.7566812576166715E-4 0.0 0.0 3.756681257616671E-5 0.0 14-15 5.259353760663339E-4 0.0 0.0 7.513362515233342E-5 0.0 16-17 6.010690012186674E-4 0.0 0.0 1.1270043772850014E-4 0.0 18-19 7.513362515233343E-4 0.0 0.0 1.5026725030466685E-4 0.0 20-21 8.264698766756676E-4 0.0 0.0 2.62967688033167E-4 0.0 22-23 9.016035018280011E-4 0.0 0.0 7.88903064099501E-4 0.0 24-25 9.767371269803345E-4 0.0 0.0 0.0015026725030466686 0.0 26-27 9.767371269803345E-4 0.0 0.0 0.00274237731806017 0.0 28-29 0.0012397048150135015 0.0 0.0 0.0053720541983918405 0.0 30-31 0.0012772716275896681 0.0 0.0 0.00935413633146551 0.0 32-33 0.0012772716275896681 0.0 0.0 0.014425656029248017 0.0 34-35 0.0013899720653181684 0.0 0.0 0.019910410665368358 0.0 36-37 0.0016529397533513353 0.0 0.0 0.026785137366806863 0.0 38-39 0.001953474253960669 0.0 0.0 0.03752924576359055 0.0 40-41 0.001953474253960669 0.0 0.0 0.058115859055329906 0.0 42-43 0.0020286078791130024 0.0 0.0 0.07599766184158525 0.0 44-45 0.0021037415042653358 0.0 0.0 0.09635887425786763 0.0 46-47 0.002254008754570003 0.0 0.0 0.13741940040361783 0.0 48-49 0.0023291423797223362 0.0 0.0 0.16604531158665686 0.0 50-51 0.0023291423797223362 0.0 0.0 0.19929194071656442 0.0 52-53 0.0023291423797223362 0.0 0.0 0.2374598222939498 0.0 54-55 0.0023291423797223362 0.0 0.0 0.2860712777675095 0.0 56-57 0.002479409630027003 0.0 0.0 0.4054210413219912 0.0 58-59 0.002479409630027003 0.0 0.0 0.5276258826322615 0.0 60-61 0.002479409630027003 0.0 0.0 0.6305213822783822 0.0 62-63 0.002479409630027003 0.0 0.0 0.7474668698279892 0.0 64-65 0.002479409630027003 0.0 0.0 0.857424930238429 0.0 66-67 0.00262967688033167 0.0 0.0 1.0211786662579398 0.0 68-69 0.0027799441306363367 0.0 0.0 1.226105628860929 0.0 70-71 0.0028926445683648367 0.0 0.0 1.339670103278681 0.0 72-73 0.00296777819351717 0.0 0.0 1.4627765480907793 0.0 74-75 0.003005345006093337 0.0 0.0 1.6723993622657898 0.0 76-77 0.003005345006093337 0.0 0.0 1.8929916857130407 0.0 78-79 0.003042911818669504 0.0 0.0 2.115011548038186 0.0 80-81 0.0036064140073120044 0.0 0.0 2.315731027632645 0.0 82-83 0.0036815476324643377 0.0 0.0 2.598646693143756 0.0 84-85 0.0037566812576166715 0.0 0.0 2.977169896661212 0.0 86-87 0.003831814882769005 0.0 0.0 3.4106157801650236 0.0 88-89 0.003906948507921338 0.0 0.0 3.9069485079213377 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATCA 75 3.0800083E-4 31.664389 2 TCGACCG 40 4.4941404E-4 29.68871 42-43 ATCGGCG 35 0.008399547 27.138863 50-51 GAGTACT 10820 0.0 23.900133 12-13 GTACATG 11140 0.0 23.499537 1 AGTACTT 11105 0.0 23.158457 12-13 TACATGG 11280 0.0 22.77983 2 GTATCAA 21795 0.0 22.627335 1 CATATAG 315 0.0 22.61657 3 GTACTTT 11730 0.0 22.04806 14-15 ACATGGG 11325 0.0 21.765827 3 GGTATCA 16735 0.0 21.235807 1 GCTTATA 875 0.0 21.17627 1 CATGGGA 7950 0.0 20.012028 4 TACTTTT 12815 0.0 19.921883 14-15 ACTTTTT 13320 0.0 19.48788 16-17 ATCAACG 25470 0.0 19.244064 3 TATCAAC 25600 0.0 19.184166 2 TCAACGC 25660 0.0 19.081629 4 CAACGCA 25820 0.0 18.963383 5 >>END_MODULE