FastQCFastQC Report
Thu 26 May 2016
SRR937109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391443
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136030.9776181992363323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97830.7030830583789633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65940.4738965232496049No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA58420.41985190913318043No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA45690.32836415146003106No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT36110.25951476273192653No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT21440.15408464450214632No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20180.1450292969241284No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA19930.143232600976109No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19300.1387049271871No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17410.12512190582007313No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG16330.11736017932462918No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC15710.112904373373541No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15080.10837669958453204No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14790.10629253228482949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG115250.025.3595281
GTATCAA211850.025.2814771
GAGTACT96500.024.88674512-13
TACATGG116450.024.771722
AGTACTT98300.024.4068712-13
ACATGGG117250.023.8739933
CATGGGG30450.022.6309784
GTACTTT107100.022.50992214-15
ATGGGAT28950.021.8336185
ATCAACG245950.021.7191143
TATCAAC246650.021.6566942
TCAACGC248350.021.528364
CAACGCA249150.021.440165
CATGGGA83750.021.3366184
AACGCAG251550.021.2522016
TACTTTT118250.020.26690314-15
ACTTTTT122500.020.14580716-17
GTATAGG2601.4822945E-820.1060311
ACGCAGA266000.020.007677
CGCAGAG267450.019.810368
Produced by FastQC (version 0.11.5)