##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937109_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1391443 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.770630201883943 33.0 31.0 34.0 30.0 34.0 2 31.8495015606101 33.0 31.0 34.0 30.0 34.0 3 31.82906234750543 33.0 31.0 34.0 30.0 34.0 4 35.439441644393625 37.0 35.0 37.0 33.0 37.0 5 35.45620409891027 37.0 35.0 37.0 33.0 37.0 6 35.56752737984955 37.0 35.0 37.0 33.0 37.0 7 35.51022859003208 37.0 35.0 37.0 33.0 37.0 8 35.48009656162703 37.0 35.0 37.0 33.0 37.0 9 37.06437489713916 39.0 37.0 39.0 33.0 39.0 10-11 36.94459923978201 39.0 37.0 39.0 33.0 39.0 12-13 36.85052639597885 39.0 37.0 39.0 33.0 39.0 14-15 37.922890481320465 40.0 37.5 41.0 33.0 41.0 16-17 37.87860012950584 40.0 37.5 41.0 33.0 41.0 18-19 37.92788493671678 40.0 38.0 41.0 33.0 41.0 20-21 37.86506238487671 40.0 37.5 41.0 32.5 41.0 22-23 37.74166135443565 40.0 37.0 41.0 32.0 41.0 24-25 37.82075694081611 40.0 37.0 41.0 33.0 41.0 26-27 37.73880712325263 40.0 37.0 41.0 32.5 41.0 28-29 37.62471621187501 40.0 37.0 41.0 32.0 41.0 30-31 37.38487670713066 39.5 37.0 41.0 32.0 41.0 32-33 37.10735006751983 39.0 36.0 41.0 31.5 41.0 34-35 36.88424930090561 39.0 35.0 41.0 31.0 41.0 36-37 36.653776690816656 39.0 35.0 41.0 30.5 41.0 38-39 36.44591442121596 39.0 35.0 41.0 30.0 41.0 40-41 36.201759612143654 39.0 35.0 41.0 30.0 41.0 42-43 35.89869832971958 39.0 35.0 40.0 29.5 41.0 44-45 35.503915000470734 39.0 35.0 40.0 27.5 41.0 46-47 34.97092550682996 38.0 35.0 40.0 25.0 41.0 48-49 34.55212610218313 38.5 34.0 40.0 23.0 41.0 50-51 33.765568550059186 38.0 33.5 39.5 21.5 40.5 52-53 33.908136014195335 38.0 33.5 39.5 22.0 40.5 54-55 34.30312021405116 38.0 34.0 40.0 21.5 41.0 56-57 34.23143779515223 38.0 34.0 40.0 20.5 41.0 58-59 33.994824078312945 38.0 34.0 40.0 19.5 41.0 60-61 33.72444792923605 38.0 34.0 40.0 18.0 41.0 62-63 33.38745532515526 37.5 33.0 40.0 14.5 41.0 64-65 33.03025240703356 37.0 33.0 40.0 12.5 41.0 66-67 32.66651742112325 36.5 32.5 39.5 8.0 41.0 68-69 32.28498364647348 36.0 32.0 39.0 7.5 41.0 70-71 31.88766733527712 35.5 32.0 39.0 4.5 40.5 72-73 31.479635529446767 35.0 31.0 38.0 2.0 40.0 74-75 31.04249185917066 35.0 31.0 37.0 2.0 39.5 76-77 30.600270007467067 35.0 31.0 37.0 2.0 39.0 78-79 30.183441218936025 35.0 30.0 36.0 2.0 39.0 80-81 29.759680777437524 34.5 30.0 36.0 2.0 37.5 82-83 29.356662831319717 34.0 29.5 35.0 2.0 37.0 84-85 28.98065713076281 34.0 29.0 35.0 2.0 37.0 86-87 28.620693409647394 34.0 29.0 35.0 2.0 36.0 88-89 28.247440606622046 34.0 29.0 35.0 2.0 36.0 90-91 27.84281713300509 34.0 27.5 35.0 2.0 35.5 92-93 27.51835899853605 34.0 27.0 35.0 2.0 35.0 94-95 27.1572730611315 34.0 26.0 35.0 2.0 35.0 96-97 26.661707306731213 34.0 25.0 35.0 2.0 35.0 98-99 26.044641785542062 33.0 24.5 35.0 2.0 35.0 100-101 23.728980633773716 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3958271549048149 1101 2 0.4472079991416784 1101 3 0.2279479574324732 1101 4 0.2990745408630815 1101 5 0.35078265733676517 1101 6 0.2611546136429652 1101 7 0.20589297753693359 1101 8 0.23791787340231707 1101 9 0.31218178093998716 1101 10-11 0.3489967382532697 1101 12-13 0.40745674740831106 1101 14-15 0.4413970078930305 1101 16-17 0.4931058989069115 1101 18-19 0.5481000587838096 1101 20-21 0.5454350615667067 1101 22-23 0.509752096226336 1101 24-25 0.597466832767843 1101 26-27 0.5940931638029809 1101 28-29 0.46482957987000617 1101 30-31 0.3567258725357618 1101 32-33 0.5331471706050976 1101 34-35 0.4609423791078413 1101 36-37 0.5397346789957922 1101 38-39 0.6853467834395346 1101 40-41 0.5820760019315259 1101 42-43 0.6908553902974504 1101 44-45 0.48525106801126583 1101 46-47 0.6728133264094893 1101 48-49 0.7343359039821635 1101 50-51 0.8203564263929621 1101 52-53 0.8431847196764934 1101 54-55 0.789034654862661 1101 56-57 0.752382309976042 1101 58-59 0.6184996484278784 1101 60-61 0.7233501811810044 1101 62-63 0.8637500029538501 1101 64-65 0.770638621299085 1101 66-67 0.6894609526165283 1101 68-69 0.7185571642196962 1101 70-71 0.6181886712662532 1101 72-73 0.556883455283927 1101 74-75 0.6213960417888735 1101 76-77 0.6329191854644911 1101 78-79 0.6323944249670248 1101 80-81 0.6107184083229136 1101 82-83 0.4477867365853747 1101 84-85 0.5273577945381724 1101 86-87 0.5936009416874519 1101 88-89 0.7878831005438549 1101 90-91 0.7559262540335219 1101 92-93 0.9086932041907367 1101 94-95 0.9916612535809257 1101 96-97 1.0511745273954247 1101 98-99 0.850186056457769 1101 100-101 1.051894890616051 1102 1 0.2184357490878419 1102 2 0.19957096188854706 1102 3 0.20437813334192256 1102 4 0.25640118399736167 1102 5 0.3646234928337222 1102 6 0.10998334260238352 1102 7 0.2242817870121243 1102 8 0.25329463468473534 1102 9 0.3766819122156946 1102 10-11 0.4088890921725792 1102 12-13 0.37537806950054886 1102 14-15 0.4171569483813755 1102 16-17 0.5913468044175758 1102 18-19 0.5910753497783006 1102 20-21 0.5608847537980992 1102 22-23 0.5650804313586377 1102 24-25 0.18992285540560516 1102 26-27 0.4799584165816455 1102 28-29 0.48992730734163814 1102 30-31 0.2742548261225082 1102 32-33 0.5785007398457438 1102 34-35 0.4834153217257722 1102 36-37 0.38743079031759464 1102 38-39 0.465789620161722 1102 40-41 0.699618083089085 1102 42-43 0.9052957181949992 1102 44-45 0.8636897622555608 1102 46-47 0.9838528841562137 1102 48-49 0.8092644316521671 1102 50-51 0.7533000653555888 1102 52-53 0.7266403339009386 1102 54-55 0.8072972937961111 1102 56-57 0.9511096649163875 1102 58-59 0.811182486316973 1102 60-61 0.6694636909099927 1102 62-63 0.8701642799164233 1102 64-65 0.9425653111091705 1102 66-67 0.8485875315715745 1102 68-69 0.9743500694969782 1102 70-71 0.8564737729319987 1102 72-73 0.6276534602432342 1102 74-75 0.6851945773354196 1102 76-77 0.7839628273443573 1102 78-79 0.7738367074140058 1102 80-81 0.6758173026897261 1102 82-83 0.7268460732054614 1102 84-85 0.8297083590237158 1102 86-87 0.8325300236326676 1102 88-89 0.9569064092751489 1102 90-91 0.7890339876313526 1102 92-93 0.7449209237858234 1102 94-95 0.8403878792117787 1102 96-97 0.907063701525356 1102 98-99 0.8449547194876104 1102 100-101 0.9330553678761824 1103 1 0.1404742554175904 1103 2 0.3499713014860042 1103 3 0.2674061361681801 1103 4 0.25414464828237726 1103 5 0.2964275205265494 1103 6 0.19370835803228914 1103 7 0.08493023382853693 1103 8 0.05108264320818279 1103 9 0.10190121229863536 1103 10-11 0.10190039440035292 1103 12-13 0.34232945623670474 1103 14-15 0.34784876648448915 1103 16-17 0.3877314599747237 1103 18-19 0.4580161939125631 1103 20-21 0.41865493066246273 1103 22-23 0.49044858460198526 1103 24-25 0.23136505541621943 1103 26-27 0.3013988571646635 1103 28-29 0.3710329400234258 1103 30-31 0.37077723368972215 1103 32-33 0.4743681097248995 1103 34-35 0.2976442037072786 1103 36-37 0.47124028619969494 1103 38-39 0.5194315654742425 1103 40-41 0.2922631171744996 1103 42-43 0.19381810621300843 1103 44-45 0.23125932248244396 1103 46-47 0.3088453437522105 1103 48-49 0.178477869769182 1103 50-51 0.31001228164789296 1103 52-53 0.32013959801336256 1103 54-55 0.3651901418625556 1103 56-57 0.43007682506060263 1103 58-59 0.2968379149889344 1103 60-61 0.36958112572041557 1103 62-63 0.34953918042498344 1103 64-65 0.34065779835332677 1103 66-67 0.2907508874423357 1103 68-69 0.38441853357253564 1103 70-71 0.344192339920113 1103 72-73 0.2794547091575552 1103 74-75 0.3606374364331977 1103 76-77 0.14735235065118601 1103 78-79 0.1653358793659372 1103 80-81 0.3247315543325584 1103 82-83 0.3849662504887412 1103 84-85 0.2882609226214399 1103 86-87 0.4251607449142014 1103 88-89 0.24644989366790782 1103 90-91 0.09954258788102166 1103 92-93 0.29788248727463085 1103 94-95 0.29478030417777745 1103 96-97 0.3473293890566005 1103 98-99 0.31028701392083846 1103 100-101 0.5295400734234086 1104 1 0.28182535330589786 1104 2 0.32432628537070585 1104 3 0.33810728633687503 1104 4 0.3167302102696823 1104 5 0.3133276973097452 1104 6 0.25489124240098704 1104 7 0.3145617648356591 1104 8 0.37686900503163656 1104 9 0.29897943886138734 1104 10-11 0.4949522894299392 1104 12-13 0.4461313054535765 1104 14-15 0.5929973344328303 1104 16-17 0.641814518431751 1104 18-19 0.60010433758125 1104 20-21 0.5641857653162887 1104 22-23 0.6037265923416371 1104 24-25 0.5853215225550272 1104 26-27 0.5177665958489186 1104 28-29 0.5157937167915279 1104 30-31 0.36713714378900164 1104 32-33 0.5116771784870977 1104 34-35 0.32261617710356205 1104 36-37 0.5325437658103453 1104 38-39 0.47337529937242095 1104 40-41 0.5894259625215454 1104 42-43 0.5400608570241658 1104 44-45 0.48660930469633 1104 46-47 0.5950138105891938 1104 48-49 0.4669103606874927 1104 50-51 0.3695977843481444 1104 52-53 0.4350092608317695 1104 54-55 0.5067015842625011 1104 56-57 0.7426367858233576 1104 58-59 0.9174025028361754 1104 60-61 0.7910389268177624 1104 62-63 0.6685826513042628 1104 64-65 0.731562222244925 1104 66-67 0.5649275385865806 1104 68-69 0.7384437197877602 1104 70-71 0.5562988221501683 1104 72-73 0.5649568704398824 1104 74-75 0.7241596663807002 1104 76-77 0.6722552681128384 1104 78-79 0.6462336604134826 1104 80-81 0.7367219552207871 1104 82-83 0.6564379301446586 1104 84-85 0.690973237618909 1104 86-87 0.5409604732717348 1104 88-89 0.7213105017374133 1104 90-91 0.5155577707546151 1104 92-93 0.5436737807023881 1104 94-95 0.7264004439874121 1104 96-97 0.6359946490033508 1104 98-99 0.5487398510754922 1104 100-101 0.5840787663057263 1105 1 0.10789408290388991 1105 2 0.1697825077022337 1105 3 0.1010974933752884 1105 4 0.14880215947148656 1105 5 0.25162141908231206 1105 6 0.11857232275693264 1105 7 -4.8708818047771274E-4 1105 8 0.13259274513806218 1105 9 0.24828744762586297 1105 10-11 0.15556775917907828 1105 12-13 0.1522180894271017 1105 14-15 0.024421450159699987 1105 16-17 0.24553355210343142 1105 18-19 0.25175270765682 1105 20-21 0.26416452487448083 1105 22-23 0.23367337944533517 1105 24-25 0.00170964132263407 1105 26-27 -0.079904501499378 1105 28-29 0.035737373724856525 1105 30-31 -0.13303892829434716 1105 32-33 0.14102028987644388 1105 34-35 -0.15789012795389823 1105 36-37 -0.007718050582802505 1105 38-39 0.15787229217303178 1105 40-41 0.15443289361308388 1105 42-43 0.22561428232489078 1105 44-45 0.035627213742515096 1105 46-47 -0.028745300442423627 1105 48-49 -0.11968496417947705 1105 50-51 -0.10373707680643918 1105 52-53 -0.15499291910831658 1105 54-55 0.0267710069696534 1105 56-57 0.11051844302497926 1105 58-59 0.2095773563889125 1105 60-61 0.2001927187955772 1105 62-63 0.22371137956076126 1105 64-65 0.2614972712097341 1105 66-67 0.20123388165325196 1105 68-69 0.3225614589104211 1105 70-71 0.1638103926013592 1105 72-73 0.0986788665942413 1105 74-75 0.19101759018341014 1105 76-77 0.05215594109946764 1105 78-79 0.283713817369172 1105 80-81 0.2895072478917129 1105 82-83 0.2930265475036897 1105 84-85 0.4143346493257418 1105 86-87 0.3324967161729582 1105 88-89 0.23504831643133173 1105 90-91 0.07169122780420523 1105 92-93 -0.031900270185236224 1105 94-95 -0.021882154501160045 1105 96-97 -0.1388035667995382 1105 98-99 -0.2402964477216294 1105 100-101 -0.032444620538615965 1106 1 -0.049597992723402484 1106 2 0.2182328525949906 1106 3 0.25662171890565233 1106 4 0.18383368516020226 1106 5 0.1783921978326859 1106 6 0.06928480045844765 1106 7 0.15778096034789257 1106 8 0.2089362377033268 1106 9 0.22510096215316366 1106 10-11 0.17235372094165058 1106 12-13 0.19616363960906824 1106 14-15 0.33195583205863244 1106 16-17 0.31078739689167634 1106 18-19 0.16293047560369445 1106 20-21 0.4287570388835107 1106 22-23 0.35539643299891566 1106 24-25 0.47283256860166034 1106 26-27 0.39470332437344524 1106 28-29 0.3616297067689871 1106 30-31 0.3470134140843655 1106 32-33 0.25871082558408176 1106 34-35 0.3481821821260169 1106 36-37 0.1769404139643953 1106 38-39 0.1635628794212991 1106 40-41 0.05137023451716516 1106 42-43 0.15114790686281054 1106 44-45 0.46647578735543505 1106 46-47 0.35236817056168235 1106 48-49 0.23627327645229457 1106 50-51 0.09527026487074863 1106 52-53 0.16883886227921607 1106 54-55 0.07925243802402804 1106 56-57 0.25337172544762154 1106 58-59 0.024100147371591163 1106 60-61 0.1013218524261319 1106 62-63 0.2395683571606284 1106 64-65 0.2481407302735832 1106 66-67 0.2232245527982215 1106 68-69 0.15588745486162026 1106 70-71 0.1817272060156654 1106 72-73 0.14414164423362408 1106 74-75 0.2646974340725521 1106 76-77 0.09676829825316702 1106 78-79 0.09422198655989789 1106 80-81 0.16918062343110307 1106 82-83 0.2885582607430486 1106 84-85 0.3579372037369062 1106 86-87 0.5385576085704251 1106 88-89 0.5581186207215154 1106 90-91 0.49914635165921695 1106 92-93 0.43719932922948956 1106 94-95 0.3407740422580794 1106 96-97 0.36739848967181743 1106 98-99 0.40461183858087324 1106 100-101 0.49508398451408553 1107 1 0.19478515897723625 1107 2 0.19766375750153387 1107 3 0.19266086642047142 1107 4 0.1504759978196475 1107 5 0.17408697895515246 1107 6 0.16502635441936775 1107 7 0.09553182579145414 1107 8 0.15261056730177103 1107 9 0.2430572045379762 1107 10-11 0.16693340254312972 1107 12-13 0.15422508984455874 1107 14-15 0.14453641114361204 1107 16-17 0.3039794112601726 1107 18-19 0.21915701387691655 1107 20-21 0.2880357878397106 1107 22-23 0.2630659005589848 1107 24-25 0.2022170110281678 1107 26-27 0.016373315526408305 1107 28-29 0.24542677034025218 1107 30-31 0.2391552851220453 1107 32-33 0.19093439025857606 1107 34-35 0.3465915039782814 1107 36-37 0.40510788628498773 1107 38-39 0.38013956040462205 1107 40-41 0.3327829787099006 1107 42-43 0.5539856416261273 1107 44-45 0.4351286748388219 1107 46-47 0.2609070068052617 1107 48-49 0.2586586985160011 1107 50-51 0.20459839732734508 1107 52-53 0.4158160626199532 1107 54-55 0.520049562571586 1107 56-57 0.5454534964914828 1107 58-59 0.6194188836359515 1107 60-61 0.5424525209856199 1107 62-63 0.5653229206159622 1107 64-65 0.5448897441553626 1107 66-67 0.584779689430782 1107 68-69 0.5822291799213062 1107 70-71 0.33677368857772194 1107 72-73 0.23806504558656982 1107 74-75 0.24588496759582412 1107 76-77 0.05524270774151674 1107 78-79 0.2119334474388559 1107 80-81 0.15731014439471735 1107 82-83 0.038667342596976795 1107 84-85 0.02145829660745946 1107 86-87 -0.031557103550238 1107 88-89 -0.022995741241835788 1107 90-91 0.09619268890050847 1107 92-93 0.058129754541010215 1107 94-95 0.3014556477530981 1107 96-97 0.16748075290760056 1107 98-99 0.12680399863526404 1107 100-101 0.014236202170994972 1108 1 -0.06410287600493092 1108 2 -0.05460730595439145 1108 3 0.014144663819262604 1108 4 0.10167727354885159 1108 5 0.24897249162822988 1108 6 0.045968457684580244 1108 7 -0.019717324732468455 1108 8 0.06901520606018607 1108 9 0.07517158292672121 1108 10-11 -0.04033132353685431 1108 12-13 0.11774121250820002 1108 14-15 0.3681682610959669 1108 16-17 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-1.5706660109761543 2316 62-63 -1.5553728031020384 2316 64-65 -1.593517182991782 2316 66-67 -1.8754778745757683 2316 68-69 -1.5236855098432542 2316 70-71 -1.5090337763038235 2316 72-73 -1.9619792567105456 2316 74-75 -2.0608495922486405 2316 76-77 -2.17343681177287 2316 78-79 -2.5572385381867697 2316 80-81 -2.8021191109991435 2316 82-83 -2.2249976676375205 2316 84-85 -2.2254245815540052 2316 86-87 -2.2914328239483375 2316 88-89 -2.454374647463645 2316 90-91 -2.3627816074396684 2316 92-93 -2.426903230684342 2316 94-95 -2.097729978316636 2316 96-97 -2.1539412953130643 2316 98-99 -2.2710328501057013 2316 100-101 -2.7201867937191047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2515.0 3 835.0 4 872.0 5 982.0 6 1125.0 7 1362.0 8 1698.0 9 2188.0 10 2811.0 11 4126.0 12 6059.0 13 8123.0 14 9141.0 15 12241.0 16 16533.0 17 23050.0 18 19480.0 19 9677.0 20 8756.0 21 8820.0 22 9297.0 23 10274.0 24 11506.0 25 13266.0 26 15283.0 27 18141.0 28 21452.0 29 25819.0 30 31531.0 31 39146.0 32 49142.0 33 64270.0 34 87309.0 35 124365.0 36 181971.0 37 263395.0 38 241891.0 39 42982.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.77635188369458 19.1867419646092 14.813140142736374 20.223766008959842 2 14.955842471872801 21.630755806389708 40.98021302853726 22.433188693200236 3 18.7535084976622 28.115995155487994 31.20095740207653 21.929538944773284 4 11.708877138199039 17.458688082442684 39.19082698594805 31.64160779341022 5 12.305574851255322 39.3110931816907 33.50303961203419 14.880292355019783 6 28.109939049774702 37.79287852304453 18.76442957218363 15.332752854997134 7 25.954571592869463 36.16094019551466 21.03924669350201 16.84524151811386 8 26.09250247348197 33.21052207726468 20.54751673914544 20.14945871010791 9 27.97244673272224 16.392281807065306 21.12618444614899 34.50908701406347 10-11 23.881639884106978 27.593818032428047 27.43448489534081 21.090057188124163 12-13 26.48236731586271 25.769173115310632 27.86414513214403 19.884314436682626 14-15 23.435951716466324 25.697090372585418 28.583643476745085 22.28331443420317 16-17 20.092029721509995 29.44415572093063 28.734866275488237 21.72894828207113 18-19 19.987960012866612 27.495305303524482 32.84954149213178 19.66719319147713 20-21 22.06381431809548 26.01180458145416 32.196828799067305 19.727552301383046 22-23 21.155091506166414 26.351641274840492 31.942706495309874 20.550560723683216 24-25 20.746690699677558 26.424022374273182 32.702466797396475 20.12682012865279 26-27 20.923106775881863 26.27083045113255 32.641305695348535 20.16475707763706 28-29 20.265744438024107 26.569053594478127 32.91645465182131 20.24874731567645 30-31 20.358577390521926 26.795096888625693 32.780178562830095 20.066147158022282 32-33 20.191046891400518 26.89364280203545 32.241309982439034 20.674000324124997 34-35 20.749114677753482 26.54540162457306 32.15217301469518 20.553310682978278 36-37 21.0476821544253 26.281205913573174 32.229670924356945 20.44144100764458 38-39 21.10344599591359 26.39019548798691 32.26967582527754 20.23668269082196 40-41 20.63825849792571 26.661468224522395 32.377478767538 20.32279451001389 42-43 20.39510823847068 27.08572369014436 31.75268238010725 20.766485691277715 44-45 20.737235449711683 27.401762848096737 30.984737770147003 20.876263932044573 46-47 20.880733166935332 27.315132563820438 30.0967053627062 21.70742890653803 48-49 21.26504788047722 27.497002192343363 29.733903919950183 21.504046007229235 50-51 21.477631494786348 27.99374462338737 29.441450350463512 21.087173531362765 52-53 21.223641898139082 28.277143800669307 29.324478695218936 21.17473560597268 54-55 20.6964059261953 27.948373436978386 29.630796839621237 21.724423797205077 56-57 21.215482676567206 28.61785532818902 28.659934068255954 21.506727926987818 58-59 21.28455175873402 27.950837521479748 29.036234024494277 21.728376695291956 60-61 21.322824447999633 27.673757397257713 29.565852662869034 21.437565491873617 62-63 21.868948375159505 27.527079477092897 29.331970690236943 21.272001457510655 64-65 21.48739363837172 27.77686544074922 29.56730190478004 21.168439016099015 66-67 21.06272075027421 28.136576586419103 29.294289135600504 21.50641352770618 68-69 21.091903893711358 28.28535602116256 29.204039158140944 21.41870092698514 70-71 21.125024127223526 28.303057337066207 28.922727107712916 21.649191427997355 72-73 21.193799296576977 28.155897403459036 28.592823302522934 22.05747999744105 74-75 21.0504372778613 28.43360970870786 28.766784062405314 21.749168951025528 76-77 21.351297334875667 28.144233472109693 28.8700235160144 21.634445677000244 78-79 21.012925650999875 28.207340912921946 28.95208527342696 21.82764816265122 80-81 20.826942947062797 28.613651087426234 28.668009816208535 21.891396149302434 82-83 20.909873589724697 28.603807226576894 28.39092463014255 22.09539455355586 84-85 21.06044781108235 28.02143811634405 28.398711297780928 22.519402774792674 86-87 21.227609901957965 28.046850291825997 28.575623185072306 22.149916621143728 88-89 21.53451825102928 27.6401890654829 28.51568789244888 22.30960479103894 90-91 20.880489083923727 28.001017906455406 29.028305491267464 22.090187518353407 92-93 20.90131715872153 28.30944081772181 28.71533979661197 22.07390222694469 94-95 20.690817597947767 28.352624536887127 28.57008857769317 22.386469287471936 96-97 20.859352143312577 28.10232751388968 28.181938423207995 22.856381919589747 98-99 20.97327160449451 27.984551016153496 28.2045678382206 22.837609541131393 100-101 21.39751146414659 27.987472481015313 28.32003698436505 22.294979070473044 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1139.0 1 836.5 2 628.5 3 722.5 4 664.5 5 720.5 6 1189.5 7 2354.0 8 4006.0 9 4663.5 10 3918.0 11 3385.0 12 3507.5 13 3632.5 14 3752.5 15 3860.5 16 3865.0 17 3991.5 18 4214.5 19 4349.5 20 4489.5 21 4966.5 22 5721.0 23 6677.5 24 7680.5 25 8908.0 26 11020.0 27 13449.0 28 15171.5 29 16674.5 30 18817.5 31 21550.0 32 24753.5 33 28319.0 34 32409.5 35 35238.5 36 37216.5 37 40308.0 38 41492.0 39 40507.5 40 40512.0 41 41838.5 42 43683.5 43 45283.5 44 47053.0 45 48073.0 46 47988.5 47 51015.5 48 68180.5 49 67836.5 50 49881.0 51 43809.5 52 39508.0 53 37512.0 54 35590.5 55 32320.0 56 29211.5 57 26136.5 58 23915.0 59 22103.5 60 19360.5 61 16177.5 62 12984.5 63 9300.5 64 6204.0 65 4442.0 66 3216.0 67 2419.0 68 1641.5 69 1067.0 70 781.0 71 612.5 72 450.0 73 340.0 74 249.0 75 181.0 76 140.0 77 80.5 78 44.0 79 24.0 80 12.0 81 10.5 82 7.0 83 7.0 84 6.5 85 5.5 86 6.5 87 4.5 88 2.0 89 1.0 90 2.0 91 2.0 92 1.5 93 2.5 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02493813975850969 2 0.012505003798215234 3 0.012792475149898341 4 0.02292584029672793 5 0.05785360952622566 6 0.045707944917614304 7 0.017463884614748862 8 0.049660676003257055 9 0.028172192464944665 10-11 0.02389605610865842 12-13 0.0371916061240022 14-15 0.020518267726381892 16-17 0.018829373535243627 18-19 0.017643554209550803 20-21 0.014625105016878161 22-23 0.008336669198810156 24-25 0.0016888941911382643 26-27 3.59339189603886E-5 28-29 0.0022279029755440933 30-31 0.0 32-33 3.2340527064349744E-4 34-35 3.59339189603886E-5 36-37 0.0 38-39 1.437356758415544E-4 40-41 3.59339189603886E-5 42-43 1.0780175688116582E-4 44-45 1.0780175688116582E-4 46-47 0.0 48-49 6.827444602473834E-4 50-51 0.0 52-53 4.671409464850519E-4 54-55 0.0019763655428213732 56-57 2.874713516831088E-4 58-59 0.0011498854067324353 60-61 0.003521524058118083 62-63 0.0021201012186629277 64-65 0.001617026353217487 66-67 0.01185819325692824 68-69 0.015271915558165157 70-71 0.02727384449093495 72-73 0.01767948812851119 74-75 0.02008706069885723 76-77 0.034209090850289954 78-79 0.021165078267668887 80-81 0.049013865461970056 82-83 0.03848522720657619 84-85 0.01983552326613451 86-87 0.014589171097917773 88-89 0.01286434298781912 90-91 0.025333412867073968 92-93 0.032807668010834797 94-95 0.01282840906885873 96-97 0.021704087052074716 98-99 0.029681417061280985 100-101 0.042042685183654664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1391443.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.545256909739756 #Duplication Level Percentage of deduplicated Percentage of total 1 71.11066858542046 33.809750069171756 2 14.765571871215682 14.040658160723526 3 5.764699953458215 8.222524208842069 4 2.81214399224773 5.348164343143981 5 1.5724650747302842 3.738162797982224 6 0.9470610061085859 2.70169553067776 7 0.6387242377594489 2.1257815585125486 8 0.42928644035584995 1.6328427275669248 9 0.3030853684062989 1.2969244535814541 >10 1.4823566161123616 12.642857638766522 >50 0.11122807153364288 3.66864975611814 >100 0.05680145790432537 5.041472989743085 >500 0.0030293973063256884 1.0646234172385776 >1k 0.0022720479797442663 2.086108809250482 >5k 4.544095959488533E-4 1.6001398706368095 >10k+ 1.5146986531628442E-4 0.9796436680441297 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13603 0.9776181992363323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9783 0.7030830583789633 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6594 0.4738965232496049 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5842 0.41985190913318043 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4569 0.32836415146003106 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3611 0.25951476273192653 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2144 0.15408464450214632 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2018 0.1450292969241284 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1993 0.143232600976109 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1930 0.1387049271871 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1741 0.12512190582007313 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1633 0.11736017932462918 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1571 0.112904373373541 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1508 0.10837669958453204 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1479 0.10629253228482949 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.18678379207772E-5 0.0 0.0 0.0 0.0 3 7.18678379207772E-5 0.0 0.0 0.0 0.0 4 7.18678379207772E-5 0.0 0.0 0.0 0.0 5 7.18678379207772E-5 0.0 0.0 0.0 0.0 6 2.1560351376233162E-4 0.0 0.0 0.0 0.0 7 2.1560351376233162E-4 0.0 0.0 7.18678379207772E-5 0.0 8 2.1560351376233162E-4 0.0 0.0 7.18678379207772E-5 0.0 9 2.1560351376233162E-4 0.0 0.0 7.18678379207772E-5 0.0 10-11 2.1560351376233162E-4 0.0 0.0 7.18678379207772E-5 0.0 12-13 2.515374327227202E-4 0.0 0.0 1.437356758415544E-4 0.0 14-15 2.874713516831088E-4 0.0 0.0 1.7966959480194303E-4 0.0 16-17 2.874713516831088E-4 0.0 0.0 2.515374327227202E-4 0.0 18-19 3.5933918960388605E-4 0.0 0.0 2.874713516831088E-4 0.0 20-21 4.3120702752466323E-4 0.0 0.0 4.3120702752466323E-4 0.0 22-23 4.3120702752466323E-4 0.0 0.0 0.0010420836498512694 0.0 24-25 5.749427033662176E-4 0.0 0.0 0.0014732906773759328 0.0 26-27 5.749427033662176E-4 0.0 0.0 0.0019763655428213732 0.0 28-29 8.26480136088938E-4 0.0 0.0 0.003521524058118083 0.0 30-31 9.342818929701037E-4 0.0 0.0 0.007617990819602384 0.0 32-33 9.342818929701037E-4 0.0 0.0 0.012864342987819119 0.0 34-35 0.0011139514877720467 0.0 0.0 0.017643554209550806 0.0 36-37 0.0014732906773759328 0.0 0.0 0.023716386513856477 0.0 38-39 0.0016529602721778757 0.0 0.0 0.03370601598484451 0.0 40-41 0.0016888941911382643 0.0 0.0 0.05127770235647454 0.0 42-43 0.0018685637859402072 0.0 0.0 0.06888532264706496 0.0 44-45 0.002084167299702539 0.0 0.0 0.08692414996518003 0.0 46-47 0.0023357047324252594 0.0 0.0 0.12494223622527117 0.0 48-49 0.0025872421651479795 0.0 0.0 0.15099432747155292 0.0 50-51 0.0025872421651479795 0.0 0.0 0.18717978386466422 0.0 52-53 0.0026231760841083683 0.0 0.0 0.22570094499020082 0.0 54-55 0.002730977840989534 0.0 0.0 0.27449920693840857 0.0 56-57 0.0028747135168310884 0.0 0.0 0.38359458490214837 0.0 58-59 0.0028747135168310884 0.0 0.0 0.502284319228312 0.0 60-61 0.0028747135168310884 0.0 0.0 0.5973295348785397 0.0 62-63 0.0028747135168310884 0.0 0.0 0.7066405163560419 0.0 64-65 0.0028747135168310884 0.0 0.0 0.807722630391615 0.0 66-67 0.003162184868514197 0.0 0.0 0.9636039708417807 0.0 68-69 0.003162184868514197 0.0 0.0 1.1567128513349092 0.0 70-71 0.003234052706434974 0.0 0.0 1.2668143790295399 0.0 72-73 0.00334185446331614 0.0 0.0 1.3871570736278813 0.0 74-75 0.0033777883822765286 0.0 0.0 1.5861591168305134 0.0 76-77 0.003449656220197306 0.0 0.0 1.8149144449323473 0.0 78-79 0.003521524058118083 0.0 0.0 2.0341113505907176 0.0 80-81 0.003916797166682358 0.0 0.0 2.23038241595236 0.0 82-83 0.004096466761484301 0.0 0.0 2.4962574823402752 0.0 84-85 0.004204268518365467 0.0 0.0 2.869215627230149 0.0 86-87 0.004312070275246632 3.59339189603886E-5 0.0 3.2696632201247198 0.0 88-89 0.004312070275246632 7.18678379207772E-5 0.0 3.7410443690470974 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11525 0.0 25.359528 1 GTATCAA 21185 0.0 25.281477 1 GAGTACT 9650 0.0 24.886745 12-13 TACATGG 11645 0.0 24.77172 2 AGTACTT 9830 0.0 24.40687 12-13 ACATGGG 11725 0.0 23.873993 3 CATGGGG 3045 0.0 22.630978 4 GTACTTT 10710 0.0 22.509922 14-15 ATGGGAT 2895 0.0 21.833618 5 ATCAACG 24595 0.0 21.719114 3 TATCAAC 24665 0.0 21.656694 2 TCAACGC 24835 0.0 21.52836 4 CAACGCA 24915 0.0 21.44016 5 CATGGGA 8375 0.0 21.336618 4 AACGCAG 25155 0.0 21.252201 6 TACTTTT 11825 0.0 20.266903 14-15 ACTTTTT 12250 0.0 20.145807 16-17 GTATAGG 260 1.4822945E-8 20.106031 1 ACGCAGA 26600 0.0 20.00767 7 CGCAGAG 26745 0.0 19.81036 8 >>END_MODULE