FastQCFastQC Report
Thu 26 May 2016
SRR937109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391443
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149361.0734180271847285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110930.7972299260551815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79540.5716367828218618No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60860.4373876615858501No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46660.33533533173834645No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31700.22782104620886373No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.17291401803738995No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG21440.15408464450214632No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT21330.15329409828501778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.14955697071313737No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20720.14891016017185035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19030.13676449556323902No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA18190.13072759717789373No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17520.12591245203720167No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG17380.12490630230631079No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC16640.11958808230017329No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG15220.10938284931542291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118850.024.2243161
CACGTCT1201.712566E-423.7469354
AGTACTT112500.023.68361312-13
TACATGG120900.023.5710092
GTATCAA216050.023.1773831
GAGTACT111200.023.14899312-13
ACATGGG121350.022.5825843
CATGGGG33050.021.6991674
GTACTTT121650.021.18072514-15
TACTTTT131200.020.23630314-15
GTATAGG3302.7284841E-1120.1554361
GGTATCA164950.020.0751381
ATCAACG255900.019.5246413
TATCAAC257450.019.4631332
TCAACGC258400.019.3541224
CCTATAC2702.3032044E-819.3493563
CATGGGA87150.019.1828384
CAACGCA261250.019.1639185
ACAGCGT2251.6679132E-619.0002788
ACTTTTT136300.018.9222516-17
Produced by FastQC (version 0.11.5)