##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937109_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1391443 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08629530638337 31.0 31.0 34.0 30.0 34.0 2 32.1875621207624 33.0 31.0 34.0 30.0 34.0 3 32.26910840041597 34.0 31.0 34.0 30.0 34.0 4 35.9629708151897 37.0 35.0 37.0 35.0 37.0 5 35.87455109551739 37.0 35.0 37.0 35.0 37.0 6 35.936179203891214 37.0 35.0 37.0 35.0 37.0 7 35.87566935907544 37.0 35.0 37.0 35.0 37.0 8 35.8517136526613 37.0 35.0 37.0 35.0 37.0 9 37.482959776289796 39.0 37.0 39.0 35.0 39.0 10-11 37.40842312620783 39.0 37.0 39.0 34.0 39.0 12-13 37.30444330094729 39.0 37.0 39.0 34.0 39.0 14-15 38.392763124324894 40.0 38.0 41.0 34.0 41.0 16-17 38.335407558915456 40.0 38.0 41.0 33.5 41.0 18-19 38.57164971903269 40.0 38.0 41.0 34.0 41.0 20-21 38.626362344702585 40.0 38.0 41.0 34.0 41.0 22-23 38.547177642203096 40.0 38.0 41.0 34.0 41.0 24-25 38.42515503689336 40.0 38.0 41.0 34.0 41.0 26-27 38.19704508197605 40.0 37.5 41.0 33.5 41.0 28-29 38.07771716124915 40.0 37.0 41.0 33.0 41.0 30-31 37.97252025415342 40.0 37.0 41.0 33.0 41.0 32-33 37.705746480452305 40.0 36.5 41.0 33.0 41.0 34-35 37.504265356180596 40.0 36.0 41.0 33.0 41.0 36-37 37.27355666024408 40.0 36.0 41.0 32.5 41.0 38-39 37.06403639962255 39.5 35.0 41.0 32.0 41.0 40-41 36.80528702936448 39.0 35.0 41.0 31.0 41.0 42-43 36.46977813679756 39.0 35.0 41.0 30.5 41.0 44-45 36.0382527347509 39.0 35.0 41.0 30.0 41.0 46-47 35.44356254622001 39.0 35.0 40.5 27.0 41.0 48-49 34.89798072935794 39.0 35.0 40.0 24.0 41.0 50-51 34.541668972426464 38.5 34.5 40.0 23.0 41.0 52-53 34.26293710917371 38.0 34.0 40.0 22.0 41.0 54-55 33.974602265417985 38.0 34.0 40.0 20.0 41.0 56-57 33.70783855321418 38.0 33.5 40.0 18.5 41.0 58-59 33.36741857194294 38.0 33.0 40.0 17.5 41.0 60-61 33.017203363702286 37.0 33.0 40.0 13.5 41.0 62-63 32.64178590139876 36.5 32.0 39.5 11.5 41.0 64-65 32.22560536076577 36.0 31.5 39.0 8.0 40.0 66-67 31.835762586034782 35.5 31.0 39.0 7.0 40.0 68-69 31.544945067818084 35.0 31.0 38.5 4.5 40.0 70-71 31.095444800829068 35.0 31.0 38.0 2.0 40.0 72-73 30.595228478636926 35.0 30.0 37.0 2.0 39.0 74-75 30.08091707673257 34.0 29.5 36.5 2.0 39.0 76-77 28.655172004889888 32.5 28.0 35.0 2.0 37.5 78-79 29.37521120160869 34.0 29.0 36.0 2.0 38.0 80-81 29.15850882860455 34.0 29.0 35.0 2.0 37.0 82-83 28.857117395394567 34.0 29.0 35.0 2.0 37.0 84-85 28.52117334306903 34.0 29.0 35.0 2.0 36.0 86-87 28.156351356110168 34.0 28.5 35.0 2.0 36.0 88-89 27.740350125732782 33.5 27.0 35.0 2.0 35.5 90-91 27.270663979767768 33.0 26.0 35.0 2.0 35.0 92-93 26.82354900631934 33.0 25.5 35.0 2.0 35.0 94-95 26.264920302161137 32.5 25.0 34.5 2.0 35.0 96-97 25.592659203431257 32.0 23.5 34.0 2.0 35.0 98-99 24.78862195576822 32.0 10.5 34.0 2.0 35.0 100-101 21.524548616076977 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10738884544645089 1101 2 -0.010282000096722754 1101 3 -0.0264390019484253 1101 4 0.08149934957474159 1101 5 0.18141852961968397 1101 6 0.046299813990636096 1101 7 0.18081246398373452 1101 8 0.09850937620868194 1101 9 0.15426585998958586 1101 10-11 0.23671338472615133 1101 12-13 0.24495631709060461 1101 14-15 0.28362315305189867 1101 16-17 0.2761540314568407 1101 18-19 0.15374745228456987 1101 20-21 0.2096811995749519 1101 22-23 0.2790191252467764 1101 24-25 0.19794043836100883 1101 26-27 0.2961211475595178 1101 28-29 0.3997501898815443 1101 30-31 0.40337057990434744 1101 32-33 0.45771330408374666 1101 34-35 0.30833916252728955 1101 36-37 0.27098399175930155 1101 38-39 0.3055198759490523 1101 40-41 0.5060499095755091 1101 42-43 0.48294116265828535 1101 44-45 0.4528103507822365 1101 46-47 0.4470613799398464 1101 48-49 0.519870605045945 1101 50-51 0.44730477093765586 1101 52-53 0.4950499865321376 1101 54-55 0.5602145490500092 1101 56-57 0.45403871107100713 1101 58-59 0.47610557406076737 1101 60-61 0.6710690903548837 1101 62-63 0.6352630802605361 1101 64-65 0.5165025026650909 1101 66-67 0.2215566143473886 1101 68-69 0.22948144223162004 1101 70-71 0.27224606895276793 1101 72-73 0.3393516362059579 1101 74-75 0.6329562224762881 1101 76-77 0.4420238696415346 1101 78-79 0.5078631181067905 1101 80-81 0.8007350381014575 1101 82-83 0.4932215834646634 1101 84-85 0.46602192049845215 1101 86-87 0.11741383107047554 1101 88-89 0.1507359116358451 1101 90-91 0.3462542211386257 1101 92-93 0.507434795704949 1101 94-95 0.2636801570329439 1101 96-97 0.12927001174400843 1101 98-99 0.43185064772852044 1101 100-101 0.9241070303359393 1102 1 0.2934094411464372 1102 2 0.2761003331101932 1102 3 0.2572755171448975 1102 4 0.15545716631385176 1102 5 0.18578709346343913 1102 6 0.24403749595335 1102 7 0.1676411345983979 1102 8 0.22825791029660536 1102 9 0.19233873259380374 1102 10-11 0.3301576217225701 1102 12-13 0.21234888980275457 1102 14-15 0.3669154019506422 1102 16-17 0.43781204365090076 1102 18-19 0.27121806111212976 1102 20-21 0.3183614411222564 1102 22-23 0.26019637662516715 1102 24-25 0.3436871054166346 1102 26-27 0.3858356958252216 1102 28-29 0.25610247553748167 1102 30-31 0.4170670121223239 1102 32-33 0.354446726879047 1102 34-35 0.37130482663651065 1102 36-37 0.3132985283560501 1102 38-39 0.30918028027822686 1102 40-41 0.47428921060529206 1102 42-43 0.5314373264903693 1102 44-45 0.41281137181552907 1102 46-47 0.345335104377547 1102 48-49 0.3711184535829446 1102 50-51 0.3802914391601817 1102 52-53 0.3615542602857502 1102 54-55 0.481339727730834 1102 56-57 0.17754070937900224 1102 58-59 0.4616127358799531 1102 60-61 0.5399137182611824 1102 62-63 0.6253080057251026 1102 64-65 0.5291567516221747 1102 66-67 0.35689676429342043 1102 68-69 0.26382608472410496 1102 70-71 0.2143097230850337 1102 72-73 0.4523683519794659 1102 74-75 0.4122358105622901 1102 76-77 0.3507952748750647 1102 78-79 0.3956552065574428 1102 80-81 0.6518158747653047 1102 82-83 0.48359615681861 1102 84-85 0.5873294265517188 1102 86-87 0.6041411276660611 1102 88-89 0.4971841744206422 1102 90-91 0.6564434040202691 1102 92-93 0.5100712495104851 1102 94-95 0.43254520560495635 1102 96-97 0.6015708373223525 1102 98-99 0.755060192931122 1102 100-101 0.9173703985788855 1103 1 0.22441265000725252 1103 2 0.19028983742637706 1103 3 0.17500761496906847 1103 4 0.12783764392862906 1103 5 0.0983700728166923 1103 6 0.1005792153330276 1103 7 0.04803915621980792 1103 8 0.17870698324582435 1103 9 0.19100659455413904 1103 10-11 0.20064280482871055 1103 12-13 0.18946271639022427 1103 14-15 0.33197156399672423 1103 16-17 0.2803806264180082 1103 18-19 0.2956486209731679 1103 20-21 0.2670671447396913 1103 22-23 0.3123267502102962 1103 24-25 0.2901233568863191 1103 26-27 0.29854155583622344 1103 28-29 0.2252004337636464 1103 30-31 0.3132349508830714 1103 32-33 0.24037856721238882 1103 34-35 0.3243839645728386 1103 36-37 0.14462388003211402 1103 38-39 0.12901489408387334 1103 40-41 0.2613614672247948 1103 42-43 0.47554792187508355 1103 44-45 0.47521484766249245 1103 46-47 0.3613371682140496 1103 48-49 0.415462661854491 1103 50-51 0.2647965373111063 1103 52-53 0.16024502273230468 1103 54-55 0.19705776968341127 1103 56-57 0.38125379979962304 1103 58-59 0.45157562033583076 1103 60-61 0.23382009437094808 1103 62-63 0.3083446800159564 1103 64-65 0.12809732486044823 1103 66-67 0.156240679327027 1103 68-69 0.007410806748509913 1103 70-71 0.0841407063257904 1103 72-73 0.265817360695614 1103 74-75 0.3976353542282105 1103 76-77 0.24187344093710195 1103 78-79 0.36355752202002023 1103 80-81 0.4301374227264638 1103 82-83 0.37533585369437006 1103 84-85 0.3939630969690384 1103 86-87 0.1684202790075524 1103 88-89 0.12416387956291786 1103 90-91 0.1251608511187534 1103 92-93 0.14171239051324847 1103 94-95 0.04969410903472493 1103 96-97 0.05748286314963025 1103 98-99 0.10387195093442259 1103 100-101 0.6706857326886002 1104 1 0.2099068614356412 1104 2 0.1952241365995917 1104 3 0.16980719780385556 1104 4 0.11470084715384132 1104 5 0.11283813326792114 1104 6 0.031809610184758697 1104 7 0.1279116082242453 1104 8 0.1366286758371018 1104 9 0.1438531812371906 1104 10-11 0.21763965992038692 1104 12-13 0.11098350997428952 1104 14-15 0.044346185368084434 1104 16-17 0.2594287189920337 1104 18-19 0.1925036452808584 1104 20-21 0.046124871458751215 1104 22-23 0.16065970626242176 1104 24-25 0.07175602782165669 1104 26-27 0.07322113629953009 1104 28-29 0.13601722062131927 1104 30-31 0.15186807455584983 1104 32-33 0.1301002819015551 1104 34-35 0.0673197108889596 1104 36-37 -0.0011383762172840761 1104 38-39 0.11681927916940538 1104 40-41 0.1475781721585534 1104 42-43 -0.06072227614365033 1104 44-45 -0.09464348324545568 1104 46-47 0.22368915271318457 1104 48-49 0.09729502234129228 1104 50-51 0.41610684840641454 1104 52-53 0.3005272694957668 1104 54-55 0.3671408523942361 1104 56-57 0.3867405464499001 1104 58-59 0.5053836677439136 1104 60-61 0.5963590351809174 1104 62-63 0.4237546634155862 1104 64-65 0.3084818687274691 1104 66-67 0.28929462245458026 1104 68-69 0.3767987488412352 1104 70-71 0.23756177101334686 1104 72-73 0.3095189878555473 1104 74-75 0.317153525707905 1104 76-77 0.19720065153546784 1104 78-79 0.453913675476457 1104 80-81 0.620299276362914 1104 82-83 0.6447156438182269 1104 84-85 0.49370883638630403 1104 86-87 0.2148275513722453 1104 88-89 0.3346369362952295 1104 90-91 0.4472697317793184 1104 92-93 0.5579253091606411 1104 94-95 0.5186317390619948 1104 96-97 0.3827359784144271 1104 98-99 0.2039744232042544 1104 100-101 0.6160392451766121 1105 1 0.13552339320686713 1105 2 0.14123987688488882 1105 3 0.14189558040010297 1105 4 -0.014952486477604054 1105 5 0.09330531119574914 1105 6 0.08069336907220759 1105 7 0.06995035526176707 1105 8 0.014169144468326067 1105 9 0.1181746050175434 1105 10-11 0.053516722479081125 1105 12-13 0.13628478887247297 1105 14-15 0.1734866741218255 1105 16-17 0.08516207319316749 1105 18-19 0.07698778202458101 1105 20-21 0.11679265976979991 1105 22-23 0.11866791178046299 1105 24-25 -0.10299272011020122 1105 26-27 0.11845209815616897 1105 28-29 -0.05392263539084752 1105 30-31 0.03677981493057558 1105 32-33 -0.13639879022650803 1105 34-35 0.04247258617402139 1105 36-37 0.010603954865310072 1105 38-39 0.016153559006639284 1105 40-41 0.0739045818531352 1105 42-43 0.20951203946695074 1105 44-45 0.05201338300683034 1105 46-47 -0.027922046589239358 1105 48-49 -0.0815900221518504 1105 50-51 -0.07329389165529676 1105 52-53 0.17166626023940523 1105 54-55 0.22047280696204297 1105 56-57 0.37892629970304625 1105 58-59 0.17372115600576876 1105 60-61 0.163445293732984 1105 62-63 0.20922759862105522 1105 64-65 0.20782593034710573 1105 66-67 -0.02492836864679404 1105 68-69 -0.07112024682124485 1105 70-71 -0.20080042323608538 1105 72-73 0.020538584957904504 1105 74-75 0.3758959238655777 1105 76-77 0.047176057339701316 1105 78-79 0.24787580041497037 1105 80-81 0.10337958759128085 1105 82-83 0.2861951523814774 1105 84-85 0.426488427675487 1105 86-87 0.12108593575716498 1105 88-89 0.18991127336986224 1105 90-91 0.172869774260203 1105 92-93 0.19955014706253493 1105 94-95 0.05692322499858804 1105 96-97 0.12247488866796985 1105 98-99 0.130766166261985 1105 100-101 0.6235348852964506 1106 1 0.12007838276988991 1106 2 0.1575710999155362 1106 3 0.16187187170778117 1106 4 0.1256374819967121 1106 5 0.14054588283866565 1106 6 -0.002495006223796281 1106 7 0.13393554878253866 1106 8 0.14752266455055718 1106 9 0.13467727224543324 1106 10-11 0.11092278967088731 1106 12-13 0.1392218892535837 1106 14-15 0.16841618514436618 1106 16-17 0.19699863067958745 1106 18-19 -0.05429981758485525 1106 20-21 -0.003659416029300644 1106 22-23 -0.1948328355587492 1106 24-25 -0.0543417604521963 1106 26-27 -0.026798718619730266 1106 28-29 0.09778776207335227 1106 30-31 0.09450675837045708 1106 32-33 0.23315168237247974 1106 34-35 0.20135142702513775 1106 36-37 0.23276288470991346 1106 38-39 0.3524964717397552 1106 40-41 0.36135028522532053 1106 42-43 0.2263379094299367 1106 44-45 0.4353329005864808 1106 46-47 0.5521683428002646 1106 48-49 0.6118532573787903 1106 50-51 0.5998905178751386 1106 52-53 0.5578779591046512 1106 54-55 0.1927185326685077 1106 56-57 0.40766091983424246 1106 58-59 0.40738653272487113 1106 60-61 0.3520127933535875 1106 62-63 0.5726436618327782 1106 64-65 0.18291086501935183 1106 66-67 0.29809003708681914 1106 68-69 0.4207508285741177 1106 70-71 0.24133787603747692 1106 72-73 0.3377253825377835 1106 74-75 0.38448459809726643 1106 76-77 0.49186722871517574 1106 78-79 0.5806146268347021 1106 80-81 0.6959444214156854 1106 82-83 0.6390110248928558 1106 84-85 0.6448519623895343 1106 86-87 0.4988240320178967 1106 88-89 0.3893910708724313 1106 90-91 0.33344319246377907 1106 92-93 0.45773329671076723 1106 94-95 0.5310263821488483 1106 96-97 0.5037725100676447 1106 98-99 0.458005356329533 1106 100-101 0.4451960542953337 1107 1 0.11924260582046742 1107 2 0.13425551543213743 1107 3 0.15504544713527935 1107 4 0.06287096170967743 1107 5 0.08841625817882459 1107 6 0.13422809820742998 1107 7 0.09456019438061247 1107 8 0.04010761786352646 1107 9 0.013846709317341777 1107 10-11 0.10601383239848161 1107 12-13 0.1072796188393994 1107 14-15 0.14718894765190527 1107 16-17 0.16783644138943998 1107 18-19 0.15960394720874405 1107 20-21 -0.0342682406508672 1107 22-23 -0.008160465599836186 1107 24-25 0.11803221486298554 1107 26-27 0.11200561281529531 1107 28-29 0.13629484317439733 1107 30-31 0.11441059107777818 1107 32-33 0.042443009972586765 1107 34-35 0.17795514935440337 1107 36-37 0.28364231265729245 1107 38-39 0.36023973157656286 1107 40-41 0.42937646779128613 1107 42-43 0.5400971202286513 1107 44-45 0.36463581003230416 1107 46-47 0.3817328296189757 1107 48-49 0.381218253112273 1107 50-51 0.30462428695167887 1107 52-53 0.4229607755086633 1107 54-55 0.5749853467006503 1107 56-57 0.5500752673020344 1107 58-59 0.5663446266714516 1107 60-61 0.6013206028110503 1107 62-63 0.5505039706065418 1107 64-65 0.30825823823703047 1107 66-67 0.4245554308458388 1107 68-69 0.5348875082283691 1107 70-71 0.6923247975339599 1107 72-73 0.4620465647915637 1107 74-75 0.4101273229857121 1107 76-77 0.5792720080156535 1107 78-79 0.6836397450538705 1107 80-81 0.8296167026486039 1107 82-83 0.7702254500597796 1107 84-85 0.7872086197052859 1107 86-87 0.6293909034846017 1107 88-89 0.66941979910764 1107 90-91 0.8382144966519043 1107 92-93 0.9728835339158799 1107 94-95 1.0111455524768331 1107 96-97 0.8884265279859171 1107 98-99 0.33299353532527576 1107 100-101 0.6170442459463992 1108 1 0.06540335320325141 1108 2 0.0028935540393817405 1108 3 -0.04226537875363334 1108 4 0.07139788338089659 1108 5 -0.012051878521106119 1108 6 0.053124186434708065 1108 7 0.0647687645057502 1108 8 0.06526341246338774 1108 9 0.055319434433613424 1108 10-11 0.07892299097564859 1108 12-13 -0.026942062533926503 1108 14-15 -0.03684629328922284 1108 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-1.034501282413288 2316 86-87 -1.0046596311131069 2316 88-89 -1.3428321732606072 2316 90-91 -1.1735195052500984 2316 92-93 -1.3496066549018444 2316 94-95 -1.3841930464269367 2316 96-97 -0.9284892326950747 2316 98-99 -1.1163117013949986 2316 100-101 -0.8895669558727732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 14.0 5 45.0 6 97.0 7 229.0 8 544.0 9 1185.0 10 2123.0 11 3405.0 12 5353.0 13 7400.0 14 9585.0 15 13344.0 16 18438.0 17 25708.0 18 22021.0 19 11405.0 20 9061.0 21 8654.0 22 8925.0 23 9732.0 24 11352.0 25 13026.0 26 15760.0 27 19008.0 28 23481.0 29 28961.0 30 35466.0 31 43865.0 32 55340.0 33 71537.0 34 95933.0 35 133597.0 36 197864.0 37 284659.0 38 192964.0 39 11358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.677116041544465 19.95545932392487 15.441438643709096 19.925985990821573 2 15.264743995635936 22.299572433649594 40.098953328498744 22.336730242215726 3 18.956863331316235 28.14404953724133 31.510710502372362 21.38837662907007 4 12.138440851521338 17.966004977580763 38.45069744343805 31.444856727459847 5 12.14041825644313 39.80888904540107 33.26503493136262 14.785657766793179 6 28.261596055318112 38.23174934222961 18.11026394900833 15.396390653443943 7 25.805656430051393 36.43929359664751 20.786406629664313 16.968643343636785 8 26.550997777127773 33.35652268903577 20.11932935808366 19.973150175752796 9 27.817832148231837 16.468958102457737 20.948555527287517 34.764654222022905 10-11 24.13073213647802 27.91705630232229 26.964680141771076 20.987531419428617 12-13 26.63033987019231 26.013606019075162 27.84882312822013 19.5072309825124 14-15 23.519935778900035 25.877919541080736 28.692839016761734 21.9093056632575 16-17 20.048647339488575 29.540017090171855 28.911999988501147 21.499335581838423 18-19 19.931790112476964 27.24856003544528 33.41925148793642 19.40039836414133 20-21 21.8439381944971 25.777914008578946 33.01414121232876 19.364006584595195 22-23 21.036094114062056 26.0503313885547 32.828560504463795 20.085013992919453 24-25 20.728086760611568 26.13118240694786 33.4875562118673 19.653174620573267 26-27 20.853132684732756 25.940154001011244 33.52531651710618 19.681396797149826 28-29 20.1030661107905 26.304963861101864 33.72398462884054 19.867985399267088 30-31 20.274960767889286 26.56528665224233 33.48842908771283 19.671323492155555 32-33 20.153811379282217 26.596158704364985 33.05356302863453 20.196466887718266 34-35 20.68891790752478 26.204307326997945 32.93907835247294 20.16769641300434 36-37 21.019077607763002 26.045681897080247 33.011923235323074 19.923317259833667 38-39 21.115654971825787 26.165342142648957 32.99946199634934 19.71954088917592 40-41 20.54929200407495 26.332741015971006 33.17269494817373 19.945272031780313 42-43 20.38940869298994 26.85410038355865 32.39166103103038 20.36482989242103 44-45 20.761360688148923 27.150195875792253 31.61635079554103 20.472092640517793 46-47 20.999689530717056 27.161318977003713 30.60159891680717 21.237392575472064 48-49 21.393483212123694 27.435530343375802 30.104118827849252 21.06686761665125 50-51 21.636147884504673 27.87065460433873 29.78547848855303 20.70771902260357 52-53 21.323618718122123 28.191848318615996 29.694137668592962 20.790395294668915 54-55 20.797892181277586 27.932191598192023 29.832663859404008 21.437252361126383 56-57 21.355496837147694 28.64211346020678 28.87278265241182 21.129607050233705 58-59 21.37245016578167 27.871479767161826 29.237871454613334 21.518198612443165 60-61 21.457688902176788 27.60186084886676 29.774049020847947 21.166401228108505 62-63 21.935000936135438 27.55375411872641 29.543967280898485 20.96727766423966 64-65 21.587612558630745 27.694588669376323 29.807936589500887 20.90986218249204 66-67 21.19284045618752 28.091855585896663 29.47661045356067 21.23869350435514 68-69 21.31654919784136 28.2739449186558 29.369362376772724 21.04014350673011 70-71 21.264696180125096 28.2602539971382 29.042756640096933 21.432293182639768 72-73 21.401487520509285 28.1103861243328 28.765820806170282 21.722305548987634 74-75 21.26713823963261 28.377924348293227 28.810425152314956 21.54451225975921 76-77 21.457041359222046 28.11915400055913 29.027563471877755 21.396241168341067 78-79 21.162987419937217 28.225581569173098 28.95624432229799 21.655186688591698 80-81 20.956928961426595 28.649007222227414 28.707401687078978 21.686662129267017 82-83 21.020133562478303 28.50339153241776 28.469034162582236 22.007440742521702 84-85 20.960056522534444 28.108065565852453 28.46359547000349 22.468282441609613 86-87 21.144449615216683 27.999863443698374 28.5974410067793 22.258245934305638 88-89 21.47530615764963 27.64804806531363 28.596489967228194 22.280155809808544 90-91 20.78537160138433 27.938549571825543 29.125924722794277 22.15015410399585 92-93 20.671211119415858 28.19295118725924 28.79078508595412 22.34505260737078 94-95 20.497110013529124 28.23756641039784 28.51774327721052 22.74758029886251 96-97 20.63515677863626 28.028405830224063 28.19216122989397 23.144276161245706 98-99 20.667094291212315 27.92578737717024 28.171653523500485 23.23546480811696 100-101 20.659640372563675 28.044651583970566 28.259105674696716 23.036602368769046 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1697.0 1 1223.5 2 830.0 3 885.0 4 751.0 5 764.0 6 1275.0 7 2580.5 8 4177.5 9 4767.5 10 4108.5 11 3472.5 12 3502.0 13 3714.0 14 3909.5 15 4091.0 16 4277.5 17 4462.0 18 4582.5 19 4777.0 20 5113.5 21 5530.5 22 6157.5 23 7080.0 24 8083.5 25 9553.0 26 11617.5 27 13719.0 28 15523.5 29 17139.5 30 19177.0 31 21672.5 32 24697.5 33 28290.5 34 32686.0 35 35421.0 36 37015.0 37 39758.5 38 40699.5 39 39913.0 40 40271.0 41 41742.5 42 43279.5 43 44880.0 44 46808.0 45 47832.0 46 47898.0 47 50813.0 48 68190.5 49 67657.5 50 49102.0 51 43181.0 52 39028.0 53 36814.0 54 34772.0 55 31716.5 56 28798.0 57 25849.0 58 23694.0 59 21939.5 60 19362.0 61 16093.5 62 12716.5 63 9319.0 64 6259.0 65 4345.0 66 3147.0 67 2374.0 68 1666.0 69 1094.0 70 782.5 71 621.0 72 490.0 73 366.0 74 243.0 75 174.0 76 138.5 77 67.5 78 26.0 79 16.5 80 11.0 81 7.0 82 4.5 83 3.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02551308246187591 2 0.005749427033662176 3 3.59339189603886E-4 4 2.874713516831088E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 7.18678379207772E-5 10-11 0.013367417853264561 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.79669594801943E-4 20-21 9.702158119304923E-4 22-23 0.0017966959480194302 24-25 0.011965995013809405 26-27 0.006360303655988783 28-29 4.312070275246633E-4 30-31 0.0031981187874745853 32-33 0.004563607707969352 34-35 0.0 36-37 0.009055347578017927 38-39 0.013547087448066505 40-41 0.0011139514877720467 42-43 0.0 44-45 0.0 46-47 7.18678379207772E-5 48-49 0.0037371275718804152 50-51 0.008192933522968602 52-53 0.0 54-55 0.0037371275718804152 56-57 0.004060532842523912 58-59 3.2340527064349744E-4 60-61 0.0025153743272272023 62-63 0.006072832304305674 64-65 1.0780175688116582E-4 66-67 0.002982515273712254 68-69 7.18678379207772E-4 70-71 7.18678379207772E-4 72-73 0.0 74-75 3.59339189603886E-5 76-77 0.0 78-79 0.0025153743272272023 80-81 0.0031621848685141974 82-83 0.013223682177423006 84-85 0.01002556338994842 86-87 0.005426021763018679 88-89 2.1560351376233164E-4 90-91 8.983479740097151E-4 92-93 2.1560351376233164E-4 94-95 3.59339189603886E-5 96-97 0.002766911759949923 98-99 0.0025513082461875907 100-101 2.1560351376233164E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1391443.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.52848672564563 #Duplication Level Percentage of deduplicated Percentage of total 1 69.73071014180155 31.747337110608548 2 15.254031956928626 13.889859829271984 3 6.051385333748048 8.265312505179438 4 2.952986038654777 5.377799426476434 5 1.6966842092947791 3.862373225024495 6 1.0203943897709058 2.787420745776477 7 0.6770801479306034 2.1578504169079658 8 0.43880488190900346 1.5982497792914048 9 0.3402858027688248 1.3943427888857494 >10 1.6481072144311177 13.31142072160008 >50 0.11831480381791501 3.66944087782157 >100 0.06426728897353462 5.469225109259041 >500 0.0037897036202066835 1.2448722830248213 >1k 0.002526469080137789 2.3438768216365355 >5k 3.1580863501722364E-4 1.0093559051552605 >10k+ 3.1580863501722364E-4 1.8712624540802187 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14936 1.0734180271847285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11093 0.7972299260551815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7954 0.5716367828218618 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6086 0.4373876615858501 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4666 0.33533533173834645 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3170 0.22782104620886373 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2406 0.17291401803738995 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2144 0.15408464450214632 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2133 0.15329409828501778 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2081 0.14955697071313737 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2072 0.14891016017185035 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1903 0.13676449556323902 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1819 0.13072759717789373 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1752 0.12591245203720167 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1738 0.12490630230631079 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1664 0.11958808230017329 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1522 0.10938284931542291 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.18678379207772E-5 0.0 0.0 0.0 0.0 6 7.18678379207772E-5 0.0 0.0 0.0 0.0 7 1.437356758415544E-4 0.0 0.0 0.0 0.0 8 1.437356758415544E-4 0.0 0.0 0.0 0.0 9 1.437356758415544E-4 0.0 0.0 0.0 0.0 10-11 2.1560351376233162E-4 0.0 0.0 0.0 0.0 12-13 2.515374327227202E-4 0.0 0.0 7.18678379207772E-5 0.0 14-15 4.3120702752466323E-4 0.0 0.0 1.0780175688116581E-4 0.0 16-17 4.671409464850518E-4 0.0 0.0 1.7966959480194303E-4 0.0 18-19 5.749427033662176E-4 0.0 0.0 2.1560351376233162E-4 0.0 20-21 6.468105412869949E-4 0.0 0.0 3.952731085642746E-4 0.0 22-23 7.186783792077721E-4 0.0 0.0 0.0010420836498512694 0.0 24-25 8.624140550493265E-4 0.0 0.0 0.0015451585152967099 0.0 26-27 8.624140550493265E-4 0.0 0.0 0.002084167299702539 0.0 28-29 0.0010061497308908808 0.0 0.0 0.0036652597339596376 0.0 30-31 0.001078017568811658 0.0 0.0 0.007869528252325104 0.0 32-33 0.001078017568811658 0.0 0.0 0.013223682177423007 0.0 34-35 0.0011498854067324353 0.0 0.0 0.01825443083187741 0.0 36-37 0.0015092245963363215 0.0 0.0 0.02447099881202464 0.0 38-39 0.0015810924342570985 0.0 0.0 0.03510743882429967 0.0 40-41 0.0015810924342570985 0.0 0.0 0.05346967141305824 0.0 42-43 0.001724828110098653 0.0 0.0 0.07183190400181683 0.0 44-45 0.0018326298669798188 0.0 0.0 0.09040974010433772 0.0 46-47 0.002156035137623316 0.0 0.0 0.12968551352804247 0.0 48-49 0.0022279029755440933 0.0 0.0 0.15624067963976968 0.0 50-51 0.0022279029755440933 0.0 0.0 0.19264173954664332 0.0 52-53 0.0022279029755440933 0.0 0.0 0.23119883459114027 0.0 54-55 0.002371638651385648 0.0 0.0 0.2809673123512785 0.0 56-57 0.0025153743272272023 0.0 0.0 0.3907813686942261 0.0 58-59 0.0025153743272272023 0.0 0.0 0.5108725258598448 0.0 60-61 0.0025513082461875907 0.0 0.0 0.6074988339443297 0.0 62-63 0.0025872421651479795 0.0 0.0 0.7176003616389603 0.0 64-65 0.002730977840989534 0.0 0.0 0.8201917002708699 0.0 66-67 0.0028747135168310884 0.0 0.0 0.9772229261277681 0.0 68-69 0.003018449192672643 0.0 0.0 1.1703677405398567 0.0 70-71 0.003018449192672643 0.0 0.0 1.2802177308017648 0.0 72-73 0.0031262509495538085 0.0 0.0 1.4000214166157003 0.0 74-75 0.003234052706434974 0.0 0.0 1.5987719223856098 0.0 76-77 0.0033059205443557514 0.0 0.0 1.8274194487305624 0.0 78-79 0.0033777883822765286 0.0 0.0 2.046867891821656 0.0 80-81 0.003808995409801192 0.0 0.0 2.244504446103793 0.0 82-83 0.0039527310856427465 0.0 0.0 2.51235587803453 0.0 84-85 0.00413240068044469 0.0 0.0 2.886499842250096 0.0 86-87 0.004312070275246632 0.0 0.0 3.28820512230828 0.0 88-89 0.004312070275246632 0.0 0.0 3.762928125693974 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11885 0.0 24.224316 1 CACGTCT 120 1.712566E-4 23.746935 4 AGTACTT 11250 0.0 23.683613 12-13 TACATGG 12090 0.0 23.571009 2 GTATCAA 21605 0.0 23.177383 1 GAGTACT 11120 0.0 23.148993 12-13 ACATGGG 12135 0.0 22.582584 3 CATGGGG 3305 0.0 21.699167 4 GTACTTT 12165 0.0 21.180725 14-15 TACTTTT 13120 0.0 20.236303 14-15 GTATAGG 330 2.7284841E-11 20.155436 1 GGTATCA 16495 0.0 20.075138 1 ATCAACG 25590 0.0 19.524641 3 TATCAAC 25745 0.0 19.463133 2 TCAACGC 25840 0.0 19.354122 4 CCTATAC 270 2.3032044E-8 19.349356 3 CATGGGA 8715 0.0 19.182838 4 CAACGCA 26125 0.0 19.163918 5 ACAGCGT 225 1.6679132E-6 19.000278 8 ACTTTTT 13630 0.0 18.92225 16-17 >>END_MODULE