FastQCFastQC Report
Thu 26 May 2016
SRR937108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429154
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142130.9945044410889239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96460.6749447575278802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65720.45985247216185243No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA59540.4166101063986106No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46430.32487751494940365No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT36990.2588244513887237No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT21110.14770976395825783No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20830.14575056292044103No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA19850.13889335928808233No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19570.13693415825026553No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18510.1295171828928163No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG17710.12391946564191123No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC16540.11573280416246254No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.11118465889610217No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15420.10789600001119544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG124850.026.305571
TACATGG125400.025.9646022
ATAGCGC950.001222601225.014366
GTATCAA202450.024.9089721
ACATGGG125700.024.7682133
GAGTACT92700.024.4549312-13
AGTACTT94150.023.97734512-13
TAGCCCG1000.001647314623.766145
CATGGGG33050.023.7301854
GTACTTT102800.022.05264314-15
CATGGGA90700.021.434074
TATCAAC236950.021.243592
ATCAACG237700.021.0965733
TCAACGC238500.020.9859494
CAACGCA239050.020.89795
TTGCGCA1150.003712098320.6633139
AACGCAG242200.020.5846926
GGTATCA149250.020.3490771
ATCACGC1200.004745370719.8051153
ACTTTTT116850.019.54408316-17
Produced by FastQC (version 0.11.5)