FastQCFastQC Report
Thu 26 May 2016
SRR937108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937108_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429154
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150391.052300871704519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114460.8008933956732445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79540.5565530376712376No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA62840.43970069005859413No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46790.32739648771231095No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31260.21873080157911604No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24810.17359920624369382No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT21890.15316753827789026No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG21680.1516981374995277No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.14945905059916567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20790.1454706770578958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19930.13945313101317283No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA18780.13140641246499676No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18260.12776789625190849No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG17570.12293986512300285No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC16650.116502490284462No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG15330.1072662568204686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118550.024.9244161
GAGTACT116650.024.63764612-13
TACATGG120350.024.1946952
GTATCAA227100.024.1183931
ACATGGG120400.023.1983243
AGTACTT118000.023.16817712-13
GTACTTT127250.022.73225214-15
CATGGGA87550.021.1056754
ATGGGAT30200.020.919465
GGTATCA171150.020.5395531
TATCAAC265350.020.532892
ATCAACG266200.020.4851723
TCAACGC267900.020.3374484
ACCGTGG2351.1303564E-720.2161628
ACTTTTT144900.020.17599716-17
CAACGCA270950.020.1260475
AACGCAG274000.019.8520936
TACTTTT138750.019.6499714-15
CATGGGG30900.019.0620214
ACGCAGA292450.018.5346937
Produced by FastQC (version 0.11.5)