##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937108_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1429154 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00444878578516 31.0 31.0 34.0 30.0 34.0 2 32.10965508265729 33.0 31.0 34.0 30.0 34.0 3 32.2164910149641 33.0 31.0 34.0 30.0 34.0 4 35.934731316569106 37.0 35.0 37.0 35.0 37.0 5 35.774113916344916 37.0 35.0 37.0 35.0 37.0 6 35.88092395920943 37.0 35.0 37.0 35.0 37.0 7 35.824475179021995 37.0 35.0 37.0 35.0 37.0 8 35.79101832272799 37.0 35.0 37.0 33.0 37.0 9 37.427429094415295 39.0 37.0 39.0 34.0 39.0 10-11 37.34348222794745 39.0 37.0 39.0 34.0 39.0 12-13 37.231848352241954 39.0 37.0 39.0 33.5 39.0 14-15 38.298399612637965 40.0 38.0 41.0 33.0 41.0 16-17 38.320538934222625 40.0 38.0 41.0 33.5 41.0 18-19 38.530672341819006 40.0 38.0 41.0 34.0 41.0 20-21 38.5589548082292 40.0 38.0 41.0 34.0 41.0 22-23 38.461559426066046 40.0 38.0 41.0 34.0 41.0 24-25 38.35784492084128 40.0 38.0 41.0 34.0 41.0 26-27 38.113199137391774 40.0 37.5 41.0 33.5 41.0 28-29 37.92909056686683 40.0 37.0 41.0 33.0 41.0 30-31 37.8039728398759 40.0 37.0 41.0 33.0 41.0 32-33 37.56731185022748 40.0 36.0 41.0 33.0 41.0 34-35 37.39302587404856 40.0 36.0 41.0 33.0 41.0 36-37 37.165052891430875 39.0 35.5 41.0 32.0 41.0 38-39 36.94643404419678 39.0 35.0 41.0 31.5 41.0 40-41 36.529312096527036 39.0 35.0 41.0 30.5 41.0 42-43 36.24824616521383 39.0 35.0 41.0 30.0 41.0 44-45 35.85588397051683 39.0 35.0 40.0 29.5 41.0 46-47 35.25609486451425 39.0 35.0 40.0 26.5 41.0 48-49 34.700739738334704 38.5 35.0 40.0 23.5 41.0 50-51 34.346442720658516 38.0 34.0 40.0 22.5 41.0 52-53 34.041523166852556 38.0 34.0 40.0 20.5 41.0 54-55 33.64642893627979 38.0 33.0 40.0 18.0 41.0 56-57 33.4675685755349 38.0 33.0 40.0 18.0 41.0 58-59 33.16124679355759 37.5 33.0 40.0 15.0 41.0 60-61 32.77303600591679 37.0 32.5 40.0 12.5 41.0 62-63 32.38823492779645 36.0 32.0 39.0 8.5 40.0 64-65 32.0320602258399 36.0 31.0 39.0 8.0 40.0 66-67 31.71552505888099 35.5 31.0 39.0 6.5 40.0 68-69 31.27607731567067 35.0 31.0 38.0 2.0 40.0 70-71 30.84272898511987 35.0 30.0 37.5 2.0 40.0 72-73 30.428485663546404 35.0 30.0 37.0 2.0 39.0 74-75 29.94969261535146 34.0 29.0 36.5 2.0 39.0 76-77 28.50489660316523 32.5 27.5 35.0 2.0 37.0 78-79 29.1523992515852 34.0 29.0 35.0 2.0 37.5 80-81 29.024813980858603 34.0 29.0 35.0 2.0 37.0 82-83 28.694954847413225 34.0 29.0 35.0 2.0 37.0 84-85 28.264486892245344 34.0 28.5 35.0 2.0 36.0 86-87 27.863035404162183 33.0 27.0 35.0 2.0 36.0 88-89 27.464502425910716 33.0 26.5 35.0 2.0 35.0 90-91 26.99637722736668 33.0 26.0 35.0 2.0 35.0 92-93 26.536464929601706 33.0 25.0 34.5 2.0 35.0 94-95 26.0042283756684 32.0 24.5 34.0 2.0 35.0 96-97 25.228404706560667 32.0 21.0 34.0 2.0 35.0 98-99 24.38001502987082 31.5 10.0 34.0 2.0 35.0 100-101 21.456810112835985 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16440486055861925 1101 2 0.32048758111719877 1101 3 0.22724905708026455 1101 4 0.11395955979729422 1101 5 0.006333854521130888 1101 6 0.06353045109839428 1101 7 -0.016221779607136 1101 8 0.030672333939442353 1101 9 0.14717125641244166 1101 10-11 0.17458854910455557 1101 12-13 0.2330636780987092 1101 14-15 0.31422127161309277 1101 16-17 0.27533946426780176 1101 18-19 0.06931735186815757 1101 20-21 0.03729451805762096 1101 22-23 0.09096774757217929 1101 24-25 -0.023912098182385932 1101 26-27 -0.05368282420674575 1101 28-29 0.118390274713704 1101 30-31 0.2006977966644854 1101 32-33 0.19516431707709359 1101 34-35 -0.004961806451831308 1101 36-37 0.043526199205082605 1101 38-39 0.12019930020088765 1101 40-41 0.2254107446327822 1101 42-43 0.2058421291178547 1101 44-45 0.09904828086870765 1101 46-47 -0.13640762383919736 1101 48-49 -0.1206396158171188 1101 50-51 -0.015485195901845827 1101 52-53 0.07970699933710534 1101 54-55 -0.09743491905188506 1101 56-57 -0.04311431373524499 1101 58-59 -0.022428932400259782 1101 60-61 0.06699527070722411 1101 62-63 0.02635810503218039 1101 64-65 0.08844885252235812 1101 66-67 0.09426339330097377 1101 68-69 -0.17855627863903223 1101 70-71 -0.11409502101760083 1101 72-73 -0.12256939138653422 1101 74-75 -0.015700722633116726 1101 76-77 0.055931016501236996 1101 78-79 -0.04010229660984166 1101 80-81 -0.025148414303604483 1101 82-83 0.30447151877879364 1101 84-85 0.1937284735326692 1101 86-87 -0.132496144810883 1101 88-89 0.009475634113503162 1101 90-91 0.15242005159028693 1101 92-93 -0.13748465856017233 1101 94-95 0.02565081500025812 1101 96-97 -0.19493854744606764 1101 98-99 -0.14095394219600976 1101 100-101 0.4136236377860385 1102 1 -0.14397410173976155 1102 2 -0.055327418168133136 1102 3 -0.03526592351368407 1102 4 -0.016841640849207806 1102 5 -0.15033704519409952 1102 6 0.07682607181724421 1102 7 0.08830153514476535 1102 8 0.14109855245271063 1102 9 0.14022108544126155 1102 10-11 0.12054365130244094 1102 12-13 0.15749276628904596 1102 14-15 0.13041900445824695 1102 16-17 0.060688855698359134 1102 18-19 0.04952235236292779 1102 20-21 0.03217843907003726 1102 22-23 -0.11086002232589465 1102 24-25 -0.12942495675458332 1102 26-27 -0.057140042928907064 1102 28-29 -0.31261103368312604 1102 30-31 -0.09753284401016771 1102 32-33 -0.07694030519204631 1102 34-35 0.15983434752118342 1102 36-37 0.10372069954043184 1102 38-39 0.22702467870139031 1102 40-41 0.35589034353768767 1102 42-43 0.3942540212355894 1102 44-45 0.44763708143164394 1102 46-47 0.3774562973931168 1102 48-49 0.6929694978814709 1102 50-51 0.3603146853528969 1102 52-53 0.5407004188706992 1102 54-55 0.6465508535069588 1102 56-57 0.61518177426818 1102 58-59 0.3942851499077946 1102 60-61 0.6258971794273052 1102 62-63 0.5038425472043357 1102 64-65 0.5699395728003118 1102 66-67 0.60550021312703 1102 68-69 0.8322194412540966 1102 70-71 0.5999893485880108 1102 72-73 0.4726075445460012 1102 74-75 0.5161577780980444 1102 76-77 0.5070722903739835 1102 78-79 0.2612364737170374 1102 80-81 0.6685431064053482 1102 82-83 0.7595805884976556 1102 84-85 0.6780785631413586 1102 86-87 0.3495498194995754 1102 88-89 0.40666203118445665 1102 90-91 0.291207008303239 1102 92-93 0.3807243220997414 1102 94-95 0.1864750500279122 1102 96-97 0.6977172507309746 1102 98-99 0.7140290981224027 1102 100-101 0.7046251111068393 1103 1 0.16571709820965452 1103 2 0.29478165377192056 1103 3 0.21379352859741374 1103 4 0.10827889154831638 1103 5 0.02254790762042802 1103 6 0.033995575935101385 1103 7 0.15802458726218305 1103 8 0.17905835391016467 1103 9 0.3183818005738033 1103 10-11 0.1738654056218536 1103 12-13 0.1713618957685057 1103 14-15 0.2678871918090664 1103 16-17 0.29927088095878673 1103 18-19 0.22813753391238123 1103 20-21 0.20748490751030602 1103 22-23 0.10803767925960273 1103 24-25 0.2536902326986379 1103 26-27 0.23222366456860044 1103 28-29 0.23126078307602427 1103 30-31 0.3075567846710143 1103 32-33 0.2757345260625996 1103 34-35 0.13769590519495267 1103 36-37 0.3521280911403082 1103 38-39 0.3205005168955424 1103 40-41 0.4669994787078551 1103 42-43 0.12882389841109188 1103 44-45 0.22970857887873564 1103 46-47 0.3743688896695616 1103 48-49 0.39481285318726833 1103 50-51 0.21320983402269178 1103 52-53 0.26623530608399193 1103 54-55 0.4811804040989571 1103 56-57 0.3037566861412202 1103 58-59 0.20512733545655237 1103 60-61 0.04619068569499518 1103 62-63 0.3880474680876631 1103 64-65 0.30631447202732076 1103 66-67 0.3995498645710498 1103 68-69 0.3997580906589455 1103 70-71 0.4237581397863792 1103 72-73 0.6228808171784266 1103 74-75 0.36908750573434546 1103 76-77 0.45700985064017985 1103 78-79 0.5619440581687556 1103 80-81 0.6582514180033314 1103 82-83 0.9184679756714296 1103 84-85 0.5210841273910525 1103 86-87 0.5251823181954514 1103 88-89 0.8783566771748745 1103 90-91 0.4900350777299032 1103 92-93 0.4590249338510617 1103 94-95 0.5416030119633852 1103 96-97 0.9746285784052127 1103 98-99 0.983493104382184 1103 100-101 1.4035055429684888 1104 1 0.060083529845528005 1104 2 0.1179054569299538 1104 3 0.056206374813264404 1104 4 0.08070742995898428 1104 5 -0.15574193125127067 1104 6 -0.011841510784279308 1104 7 0.08591059700786019 1104 8 0.11132230034030499 1104 9 0.10081345693586741 1104 10-11 0.10330542923377095 1104 12-13 0.1432572772949996 1104 14-15 0.281485653448982 1104 16-17 0.17114788769983136 1104 18-19 0.12121132820313818 1104 20-21 0.14672134874332698 1104 22-23 0.10182818621800749 1104 24-25 0.015695145872975047 1104 26-27 0.11618305309158927 1104 28-29 0.03719807960088417 1104 30-31 0.06782921807522513 1104 32-33 0.09084064845147566 1104 34-35 0.14947785603375507 1104 36-37 0.08378371692980835 1104 38-39 0.21042034649021701 1104 40-41 0.32043703612905716 1104 42-43 0.2001484771515365 1104 44-45 0.2834052079699205 1104 46-47 0.04876272982685492 1104 48-49 0.3022002532063439 1104 50-51 0.5337397192987225 1104 52-53 0.41748860090069684 1104 54-55 0.5864171859377549 1104 56-57 0.6169478613784065 1104 58-59 0.3900588269577341 1104 60-61 0.36860702760173325 1104 62-63 0.42381859765038854 1104 64-65 0.5245286541605019 1104 66-67 0.20990239155221246 1104 68-69 0.2361924091240688 1104 70-71 0.4350365446757358 1104 72-73 0.5128174529792986 1104 74-75 0.21163672230904496 1104 76-77 0.465726669587081 1104 78-79 0.6839198213785913 1104 80-81 0.6012398047547904 1104 82-83 0.6248132948709006 1104 84-85 0.3186621770132163 1104 86-87 0.49353287068747775 1104 88-89 0.5926540529097899 1104 90-91 0.5606225842843209 1104 92-93 0.6012112003955679 1104 94-95 0.6723276059414509 1104 96-97 0.8941449374062636 1104 98-99 0.9843170886630972 1104 100-101 1.536128918128469 1105 1 -0.010703174060608944 1105 2 0.05988173126492313 1105 3 0.0022344451555937894 1105 4 0.015596563476691472 1105 5 -0.06311358066813 1105 6 0.03537557940914837 1105 7 0.07206601044124028 1105 8 0.050068410052524825 1105 9 0.20360371320862214 1105 10-11 0.09463106077737393 1105 12-13 0.16020985886927974 1105 14-15 0.08193396706847977 1105 16-17 -0.09675085682003726 1105 18-19 0.04710421705191692 1105 20-21 0.09952543461650976 1105 22-23 0.05217910622037181 1105 24-25 -0.0044748946840798 1105 26-27 -0.002952194536447905 1105 28-29 0.04854845130600438 1105 30-31 0.13587134085535268 1105 32-33 0.10323825809707188 1105 34-35 0.10148539601661355 1105 36-37 0.06286208890076495 1105 38-39 0.014929578115008724 1105 40-41 0.25082462656308024 1105 42-43 0.3744863096931539 1105 44-45 0.17610444950837945 1105 46-47 0.2357601036688024 1105 48-49 0.1912868577804403 1105 50-51 0.026618045203605334 1105 52-53 0.3267929736281445 1105 54-55 0.4115134637390767 1105 56-57 0.29766653146930366 1105 58-59 0.28175806947354687 1105 60-61 0.22785183042486068 1105 62-63 0.278108526671474 1105 64-65 0.08069784506860245 1105 66-67 0.17481104288762594 1105 68-69 0.17666094751611183 1105 70-71 0.11735987193751285 1105 72-73 0.01459445075385446 1105 74-75 -0.12325869887152052 1105 76-77 0.06777804402782905 1105 78-79 0.23042963094711055 1105 80-81 0.028154683156927263 1105 82-83 0.1827194119198161 1105 84-85 -0.0991469927448847 1105 86-87 -0.11414547333108516 1105 88-89 -0.08706087162359566 1105 90-91 0.19586695446411895 1105 92-93 0.338784840167218 1105 94-95 0.4173679268012087 1105 96-97 0.3361614462677984 1105 98-99 0.46172776394180204 1105 100-101 0.6310336958726168 1106 1 0.038890118280676234 1106 2 0.012945415468806232 1106 3 0.03639104246727243 1106 4 0.013346744986307613 1106 5 -0.04334551663261976 1106 6 0.11552662423797244 1106 7 0.0371300477556602 1106 8 0.0157593987079494 1106 9 0.19784945291211642 1106 10-11 0.13683173204769616 1106 12-13 0.22619324465873802 1106 14-15 0.1721101079667875 1106 16-17 0.14299713756622623 1106 18-19 0.10135622626882679 1106 20-21 0.045293062668619655 1106 22-23 0.12354094181859665 1106 24-25 0.2859221204510263 1106 26-27 0.27177373944990535 1106 28-29 0.31572052242535165 1106 30-31 0.3195059291122746 1106 32-33 0.34067662139376154 1106 34-35 0.2457409543546163 1106 36-37 0.23253025508611103 1106 38-39 0.36747764888844614 1106 40-41 0.4687829991559411 1106 42-43 0.5883540049443567 1106 44-45 0.3346829634535382 1106 46-47 0.14896571668489145 1106 48-49 0.17249309054152917 1106 50-51 0.1908474085056895 1106 52-53 0.2298698994767321 1106 54-55 0.53970483523117 1106 56-57 0.5704651305573378 1106 58-59 0.6337126983742039 1106 60-61 0.41981370169445853 1106 62-63 0.4104936261279306 1106 64-65 0.33846510559751763 1106 66-67 0.3730721537084847 1106 68-69 0.34052963363868827 1106 70-71 0.32547456800122987 1106 72-73 0.291283030691055 1106 74-75 0.16005430658547937 1106 76-77 0.3740070217908773 1106 78-79 0.4074837523414665 1106 80-81 0.5020902621050567 1106 82-83 0.4996166513521736 1106 84-85 0.5927669563819755 1106 86-87 0.5085098097248348 1106 88-89 0.5919032894596619 1106 90-91 0.41285157073566836 1106 92-93 0.5884548800002491 1106 94-95 0.9344793746084115 1106 96-97 0.8654737635195886 1106 98-99 0.9372961187530251 1106 100-101 1.477122186090913 1107 1 0.13198263289511658 1107 2 0.22043545498698336 1107 3 0.18066414481589987 1107 4 0.13658598068499828 1107 5 0.021709936288552 1107 6 0.003348430256956192 1107 7 0.07022009374890104 1107 8 0.04667220174637521 1107 9 0.1499582017461023 1107 10-11 0.13132630209279483 1107 12-13 0.14893086462462435 1107 14-15 0.2271204984338624 1107 16-17 0.28673604647612194 1107 18-19 0.1642706561690872 1107 20-21 0.11235032456301752 1107 22-23 0.13726930850192076 1107 24-25 0.09887610899419741 1107 26-27 0.24205578953863238 1107 28-29 0.20001179544473757 1107 30-31 0.19403218654090182 1107 32-33 0.20065258268902397 1107 34-35 0.38368224568630893 1107 36-37 0.3413361506391581 1107 38-39 0.3236207722608313 1107 40-41 0.2665826887571754 1107 42-43 0.45432284770735265 1107 44-45 0.3778812038527235 1107 46-47 0.5538576335692511 1107 48-49 0.7134847478159756 1107 50-51 0.4717801426250787 1107 52-53 0.6975526128556453 1107 54-55 0.6451904352781099 1107 56-57 0.5746283994974277 1107 58-59 0.4095849613916229 1107 60-61 0.29076996865357785 1107 62-63 0.3934026736246068 1107 64-65 0.5326547402213428 1107 66-67 0.5404602513120977 1107 68-69 0.24084913797192797 1107 70-71 0.2009571126235805 1107 72-73 0.5361129892303573 1107 74-75 0.34089740142707115 1107 76-77 0.23969703176653567 1107 78-79 0.3978721150183411 1107 80-81 0.37462745835841815 1107 82-83 0.4360257787755195 1107 84-85 0.5302503387167654 1107 86-87 0.594980146813036 1107 88-89 0.8158479033126689 1107 90-91 0.6817582750656719 1107 92-93 0.7501335828814533 1107 94-95 0.9153445251639525 1107 96-97 0.9109364579993873 1107 98-99 0.9662099456622073 1107 100-101 1.052031282150633 1108 1 -0.06013310394576621 1108 2 0.042978481486827036 1108 3 0.02851030817362954 1108 4 -0.019548241901304664 1108 5 -0.13198334871805883 1108 6 -0.06546421609497344 1108 7 0.017087099939153916 1108 8 -0.007282294880283757 1108 9 -0.0016944640807707856 1108 10-11 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-1.869729518111317 2316 90-91 -1.676223508128686 2316 92-93 -1.7529104196842944 2316 94-95 -1.9480733067796017 2316 96-97 -1.9745085708652717 2316 98-99 -2.023929117840037 2316 100-101 -2.7806039968743868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 9.0 5 54.0 6 129.0 7 268.0 8 643.0 9 1358.0 10 2253.0 11 3722.0 12 5739.0 13 8101.0 14 10302.0 15 14469.0 16 19353.0 17 26609.0 18 22678.0 19 11705.0 20 9454.0 21 9053.0 22 9669.0 23 10405.0 24 12051.0 25 13986.0 26 17037.0 27 20541.0 28 25194.0 29 31380.0 30 38381.0 31 47485.0 32 58923.0 33 75757.0 34 101981.0 35 141151.0 36 206895.0 37 288340.0 38 175624.0 39 8449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63194316623847 19.963481606781308 15.490433342734708 19.914141884245513 2 15.264868571588512 22.35252133328202 40.12112475990218 22.26148533522729 3 18.960387587569656 28.141941912244217 31.535152412486323 21.362518087699804 4 12.121087020712954 18.028008178264905 38.44001416222464 31.4108906387975 5 12.144884375752216 39.7897435610365 33.28759397444007 14.77777808877121 6 28.273650005527745 38.28831602472512 18.082515949995592 15.355518019751546 7 25.70197270967169 36.48466817012338 20.812426398610086 17.00093272159485 8 26.53660837110626 33.40689666753897 20.088737812719973 19.96775714863479 9 27.830680130119028 16.380121652475278 20.986836258958977 34.80236195844672 10-11 24.07886064063075 27.889086830390568 26.9754344178444 21.056618111134277 12-13 26.718478681862027 26.02114022587314 27.822093498843486 19.438287593421347 14-15 23.4734490981762 25.89199246520897 28.710681657877945 21.923876778736886 16-17 19.959185644094337 29.617871831866964 28.900069551636843 21.522872972401856 18-19 19.890193394810478 27.23008587628022 33.49479305516779 19.384927673741505 20-21 21.84933337624953 25.703638966596742 33.088454471284166 19.358573185869563 22-23 20.978271099236007 26.03017842629817 32.908682079036005 20.082868395429816 24-25 20.716828447827982 26.127870383814074 33.5408470463932 19.614454121964744 26-27 20.86389051961619 25.948062870893278 33.56860121848566 19.61944539100487 28-29 20.10907155879337 26.28807892224313 33.786573660562006 19.816275858401493 30-31 20.245026164089442 26.59011085933037 33.55251902612281 19.612343950457387 32-33 20.1470942723707 26.632375923756157 33.09141822962786 20.12911157424528 34-35 20.69119213184863 26.23356195343539 33.03324904104106 20.04199687367492 36-37 21.013334658145883 26.02182582027005 33.07379369169979 19.89104582988427 38-39 21.0629202876264 26.12962222355182 33.06377542719365 19.74368206162813 40-41 20.596937538506428 26.309900783300776 33.078355617643986 20.01480606054881 42-43 20.464449597454156 26.840949260891406 32.390910986499705 20.303690155154726 44-45 20.732795295645506 27.087368382752192 31.68470077193061 20.49513554967169 46-47 20.92250477118432 27.16060742293072 30.652292180155722 21.264595625729235 48-49 21.35811258770495 27.427233972581654 30.106549161128708 21.108104278584687 50-51 21.628730456224638 27.84845201438776 29.810062964814644 20.71275456457296 52-53 21.341059156636497 28.211488465318286 29.665922982703314 20.781529395341906 54-55 20.805840378293734 27.926801450368536 29.831214830592085 21.436143340745645 56-57 21.357092902392015 28.569944173129425 28.913784797332404 21.159178127146163 58-59 21.33529673746747 27.89373827319096 29.28603390615614 21.48493108318543 60-61 21.46785877347813 27.65791595891885 29.738773268211787 21.135451999391236 62-63 21.94473093872319 27.534366485347277 29.52414505364712 20.996757522282415 64-65 21.586402286110925 27.66913526342894 29.84010798011688 20.904354470343254 66-67 21.12376312271565 27.98450271436903 29.605357297169267 21.28637686574605 68-69 21.285754569314832 28.253343064038628 29.380584164186757 21.08031820245978 70-71 21.264767539820927 28.265092208784537 29.055738104101188 21.41440214729335 72-73 21.344842750770827 28.144031218589248 28.75932766958787 21.751798361052053 74-75 21.278418036400275 28.288060401228737 28.802891309976136 21.630630252394848 76-77 21.431827604122024 28.117923034805592 29.020520903122655 21.42972845794973 78-79 21.144811748505408 28.17037655896644 29.07227502742885 21.612536665099302 80-81 20.9328931569269 28.647724060333463 28.75618210876033 21.663200673979304 82-83 21.012963268116405 28.54385480635915 28.4308858355663 22.01229608995815 84-85 21.00242420977024 28.09476844405897 28.47073262635087 22.432074719819916 86-87 21.13625700898122 28.005487183452242 28.603815488733325 22.254440318833208 88-89 21.495528193738735 27.615201097458193 28.5969237723481 22.292346936454972 90-91 20.79983990327023 27.884400085086376 29.161640319745636 22.154119691897762 92-93 20.62475422522695 28.272530462077565 28.80329901466182 22.299416298033663 94-95 20.456927664898256 28.226384280490418 28.540906018525646 22.775782036085683 96-97 20.68031999417836 27.963455226214723 28.162769364468836 23.19345541513808 98-99 20.71482574727346 27.847794363834893 28.20846442856938 23.22891546032227 100-101 20.643717812360055 28.05866547245858 28.320854791759963 22.976761923421407 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1781.0 1 1288.0 2 879.0 3 933.0 4 787.0 5 798.5 6 1333.0 7 2609.0 8 4147.5 9 4799.0 10 4199.0 11 3529.5 12 3572.5 13 3785.0 14 3975.0 15 4228.5 16 4392.5 17 4575.5 18 4799.5 19 4959.0 20 5282.0 21 5744.5 22 6399.5 23 7387.0 24 8400.0 25 9723.0 26 11853.5 27 14328.0 28 16027.0 29 17388.0 30 19617.5 31 22365.5 32 25638.5 33 29144.5 34 33276.0 35 36225.0 36 38313.0 37 41023.5 38 41809.5 39 41030.0 40 41141.0 41 42684.5 42 44526.0 43 46384.0 44 48499.5 45 49355.0 46 49229.0 47 52360.0 48 70127.5 49 69412.5 50 50221.5 51 44053.0 52 39749.5 53 37506.5 54 35603.0 55 32656.0 56 29708.0 57 26648.5 58 24247.5 59 22334.5 60 19816.5 61 16422.0 62 12951.0 63 9523.0 64 6455.5 65 4556.5 66 3252.0 67 2381.0 68 1700.0 69 1136.5 70 827.0 71 645.5 72 475.5 73 379.0 74 282.0 75 191.5 76 136.0 77 62.5 78 26.5 79 15.0 80 7.0 81 2.5 82 0.5 83 1.5 84 1.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01994186770634935 2 0.003428601816179362 3 4.198287938178811E-4 4 0.0 5 0.0029388015567251673 6 0.0 7 2.0991439690894054E-4 8 0.0 9 6.997146563631352E-5 10-11 0.0 12-13 0.005422788586814297 14-15 0.0034985732818156754 16-17 0.0 18-19 3.4985732818156756E-4 20-21 8.396575876357622E-4 22-23 0.001434415045544427 24-25 8.046718548176053E-4 26-27 2.0991439690894054E-4 28-29 3.498573281815676E-5 30-31 3.498573281815676E-5 32-33 1.7492866409078378E-4 34-35 0.0 36-37 0.0014694007783625836 38-39 0.010320791181356244 40-41 6.647289235449784E-4 42-43 0.0 44-45 6.997146563631351E-4 46-47 1.0495719845447027E-4 48-49 1.0495719845447027E-4 50-51 0.001574357976817054 52-53 5.247859922723513E-4 54-55 0.0 56-57 2.4490012972709727E-4 58-59 2.4490012972709727E-4 60-61 0.002204101167543876 62-63 0.0 64-65 1.3994293127262704E-4 66-67 3.498573281815676E-5 68-69 6.997146563631352E-5 70-71 4.198287938178811E-4 72-73 3.848430609997243E-4 74-75 0.0030787444879977945 76-77 1.0495719845447027E-4 78-79 1.3994293127262704E-4 80-81 0.002099143969089405 82-83 3.498573281815676E-5 84-85 0.001994186770634935 86-87 3.848430609997243E-4 88-89 0.0023440440988165027 90-91 0.0012594863814536432 92-93 0.0 94-95 0.0 96-97 2.0991439690894054E-4 98-99 5.947574579086649E-4 100-101 0.0023790298316346595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1429154.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.58670632155498 #Duplication Level Percentage of deduplicated Percentage of total 1 69.71906746629465 31.782626535986523 2 15.25669102384192 13.91004586285171 3 6.049351867539026 8.273100810637553 4 3.0379616820223543 5.539626680579611 5 1.6305281028790297 3.7165202887494253 6 1.0039369210983753 2.745970655248665 7 0.6608353375986897 2.1087714523411973 8 0.48363595013967725 1.7637896020450936 9 0.34183779304050976 1.4024933172851588 >10 1.6335692979729848 13.330972935289928 >50 0.11373166817902293 3.606062968012614 >100 0.061767388636837076 5.293429222773334 >500 0.003542750378470744 1.1250392122810449 >1k 0.0029266198778671363 2.5420465337035565 >5k 3.080652503018038E-4 0.9997695858480538 >10k+ 3.080652503018038E-4 1.8597343363664631 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15039 1.052300871704519 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11446 0.8008933956732445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7954 0.5565530376712376 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6284 0.43970069005859413 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4679 0.32739648771231095 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3126 0.21873080157911604 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2481 0.17359920624369382 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2189 0.15316753827789026 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2168 0.1516981374995277 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2136 0.14945905059916567 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2079 0.1454706770578958 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1993 0.13945313101317283 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1878 0.13140641246499676 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1826 0.12776789625190849 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1757 0.12293986512300285 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1665 0.116502490284462 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1533 0.1072662568204686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.3994293127262702E-4 0.0 0.0 0.0 0.0 6 1.3994293127262702E-4 0.0 0.0 0.0 0.0 7 1.3994293127262702E-4 0.0 0.0 0.0 0.0 8 1.3994293127262702E-4 0.0 0.0 0.0 0.0 9 1.3994293127262702E-4 0.0 0.0 6.997146563631351E-5 0.0 10-11 2.0991439690894054E-4 0.0 0.0 1.0495719845447026E-4 0.0 12-13 2.0991439690894054E-4 0.0 0.0 1.3994293127262702E-4 0.0 14-15 2.7988586254525403E-4 0.0 0.0 2.0991439690894054E-4 0.0 16-17 3.148715953634108E-4 0.0 0.0 2.0991439690894054E-4 0.0 18-19 4.898002594541945E-4 0.0 0.0 2.0991439690894054E-4 0.0 20-21 5.597717250905081E-4 0.0 0.0 2.4490012972709727E-4 0.0 22-23 6.647289235449784E-4 0.0 0.0 9.096290532720757E-4 0.0 24-25 9.096290532720757E-4 0.0 0.0 0.0017842723737259945 0.0 26-27 9.096290532720757E-4 0.0 0.0 0.0022390869003620323 0.0 28-29 0.0010845577173628595 0.0 0.0 0.004233273670996967 0.0 30-31 0.0011895149158173298 0.0 0.0 0.008886376135811816 0.0 32-33 0.0011895149158173298 0.0 0.0 0.013714407264717447 0.0 34-35 0.0012594863814536432 0.0 0.0 0.019032238653077273 0.0 36-37 0.0013294578470899568 0.0 0.0 0.025714513621345216 0.0 38-39 0.0015393722439988973 0.0 0.0 0.03589536187142883 0.0 40-41 0.001609343709635211 0.0 0.0 0.055417400783960305 0.0 42-43 0.0017492866409078377 0.0 0.0 0.07591904021540016 0.0 44-45 0.0022390869003620323 0.0 0.0 0.09830990921902048 0.0 46-47 0.0027288871598162272 0.0 0.0 0.13952310247880914 0.0 48-49 0.003008773022361481 0.0 0.0 0.16730177433642562 0.0 50-51 0.003008773022361481 0.0 0.0 0.2034770220703997 0.0 52-53 0.003008773022361481 0.0 0.0 0.23993215566691903 0.0 54-55 0.0030787444879977945 0.0 0.0 0.28565850846024987 6.997146563631351E-5 56-57 0.003288658884906735 0.0 0.0 0.39694812455480655 6.997146563631351E-5 58-59 0.003288658884906735 0.0 0.0 0.5103718703512707 6.997146563631351E-5 60-61 0.003288658884906735 0.0 0.0 0.6077721505170192 6.997146563631351E-5 62-63 0.0033586303505430486 0.0 0.0 0.7142337354826702 6.997146563631351E-5 64-65 0.0034286018161793622 0.0 0.0 0.8152725318615068 6.997146563631351E-5 66-67 0.003568544747451989 0.0 0.0 0.9655012685826719 6.997146563631351E-5 68-69 0.0036385162130883027 0.0 0.0 1.1606167005095323 6.997146563631351E-5 70-71 0.0037784591443609295 0.0 0.0 1.2630899119339132 6.997146563631351E-5 72-73 0.003848430609997243 0.0 0.0 1.3803620883403749 6.997146563631351E-5 74-75 0.003848430609997243 0.0 0.0 1.5805854372586858 6.997146563631351E-5 76-77 0.003848430609997243 6.997146563631351E-5 0.0 1.812505860110247 6.997146563631351E-5 78-79 0.003848430609997243 6.997146563631351E-5 0.0 2.0239596292631865 6.997146563631351E-5 80-81 0.00409333073972434 6.997146563631351E-5 0.0 2.212427771954597 6.997146563631351E-5 82-83 0.004128316472542497 6.997146563631351E-5 0.0 2.4725117097247744 6.997146563631351E-5 84-85 0.004338230869451438 6.997146563631351E-5 0.0 2.844584978245871 6.997146563631351E-5 86-87 0.004548145266360378 6.997146563631351E-5 0.0 3.2691368459942036 6.997146563631351E-5 88-89 0.004548145266360378 6.997146563631351E-5 0.0 3.742353868092592 6.997146563631351E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11855 0.0 24.924416 1 GAGTACT 11665 0.0 24.637646 12-13 TACATGG 12035 0.0 24.194695 2 GTATCAA 22710 0.0 24.118393 1 ACATGGG 12040 0.0 23.198324 3 AGTACTT 11800 0.0 23.168177 12-13 GTACTTT 12725 0.0 22.732252 14-15 CATGGGA 8755 0.0 21.105675 4 ATGGGAT 3020 0.0 20.91946 5 GGTATCA 17115 0.0 20.539553 1 TATCAAC 26535 0.0 20.53289 2 ATCAACG 26620 0.0 20.485172 3 TCAACGC 26790 0.0 20.337448 4 ACCGTGG 235 1.1303564E-7 20.216162 8 ACTTTTT 14490 0.0 20.175997 16-17 CAACGCA 27095 0.0 20.126047 5 AACGCAG 27400 0.0 19.852093 6 TACTTTT 13875 0.0 19.64997 14-15 CATGGGG 3090 0.0 19.062021 4 ACGCAGA 29245 0.0 18.534693 7 >>END_MODULE