FastQCFastQC Report
Thu 26 May 2016
SRR937107_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937107_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160490
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106080.3356441564440957No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94760.2998269255716677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76210.24113349512259177No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57300.18130100079418066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50660.1602916003531098No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46140.1459900205347905No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35200.11137513486832737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG134700.024.5552311
TACATGG136650.023.4759982
GTATCAA236800.022.2161251
ACATGGG139000.021.8126413
GAGTACT94850.021.13783612-13
AGAGTAC166750.020.04476210-11
CATGGGG68900.019.795754
GTACTTT100800.019.7719714-15
ATCAACG266950.019.528963
TCAACGC266650.019.5155964
TATCAAC269800.019.3943542
CAACGCA268250.019.3808615
AGTACTT101650.019.09282112-13
AACGCAG273050.019.091776
ACGCAGA297350.017.1956757
CGCAGAG297600.017.037528
CATGGGA80800.016.5862034
GGTATCA182450.016.3835951
GTATAGG9000.016.3689791
ACTTTTT126300.016.30637416-17
Produced by FastQC (version 0.11.5)